Durability Of Resin-dentin Bonds Research Articles

Synergistic effect of collagen cross-linking and remineralization for improving resin-dentin bond durability.

In this study, the synergistic effect of glutaraldehyde-cross-linking and remineralization on the strength and durability of resin-dentin bonds was investigated. Dentin surfaces were etched with 35% phosphoric acid. The control specimens were bonded with Adper Single Bond 2 using wet bonding without pretreatment. The experimental specimens were pretreated with 5% (v/v) glutaraldehyde solution for 3min and placed in a remineralizing solution for 0, 12, and 24 h, followed by dry bonding. After performing composite build-ups on the specimens, they were longitudinally sectioned, immediately, and after aging for 3h with sodium hypochlorite (NaOCl), to evaluate microtensile bond strength (µTBS). The cross-linked specimens exhibited µTBS values comparable with those of the control group, but the µTBS decreased significantly after NaOCl aging. The cross-linked dentin remineralized for 24 h exhibited an increase in µTBS. After aging in NaOCl, the µTBS of the specimens remineralized for 24 h did not decrease and was significantly higher than for the other experimental groups. Cross-linking with dry bonding maintained µTBS in specimens before aging in NaOCl, but the bonding durability was compromised. Remineralization of cross-linked dentin for 24 h followed by dry bonding increased the immediate µTBS and improved bond durability. Therefore, combining cross-linking with remineralization of collagen fibrils progressively increased resistance to degradation, improving bond durability.

Polyphenols Can Improve Resin-Dentin Bond Durability by Promoting Amorphous Calcium Phosphate Nanoparticles to Backfill the Dentin Matrix.

To investigate the effects of proanthocyanidins (PA), myricetin, resveratrol, and kaempferol on the modification of dentin collagen and the inhibition of matrix metalloproteinase (MMP) activity, and to evaluate their contributions to the biomimetic remineralization and resin-dentin bonding performance. Attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) and in situ zymography were applied to verify the collagen modification and MMP activity inhibition induced by these four polyphenols. Scanning electron microscopy/energy dispersive spectrometer (SEM/EDS) analysis, X-ray diffraction (XRD), ATR-FTIR, Vickers hardness numbers (VHN), and micro-computed tomography (micro-CT) were performed to characterize the remineralized dentin. Microtensile bond strength (μTBS) and nanoleakage were investigated to evaluate the effects of the four polyphenols on resin-dentin bonding durability. ATR-FTIR and in situ zymography confirmed that these four polyphenols could modify dentin collagen and inhibit MMP activity, respectively. Chemoanalytic characterization exhibited the efficacies of the four polyphenols in promoting dentin biomimetic remineralization. The surface hardness of PA-pretreated dentin was the greatest. Micro-CT results demonstrated that the PAs group possessed the highest amount of dentin surface minerals and the lowest amount of deep-layer minerals. The surface and deep-layer mineral contents of the Myr group were higher than Res and Kae groups. Treatment with these four polyphenols significantly increased the initial μTBS compared with the control group without primer conditioning. μTBS decreased significantly during aging, and the decrease was more severe in the PAs and Kae groups than in the Myr and Res groups. With or without aging, the polyphenol groups exhibited relatively less fluorescence. However, the Myr and Res groups showed less serious nanoleakage after aging. PA, myricetin, resveratrol, and kaempferol can modify dentin collagen, inhibit MMP activity, promote biomimetic remineralization, and improve resin-dentin bond durability. Compared with PA and kaempferol, myricetin and resveratrol are more effective in improving resin-dentin bonding.

The durability of resin-dentine bonds are enhanced by epigallocatechin-3-gallate-encapsulated nanohydroxyapatite/mesoporous silica.

Biomimetic nanohydroxyapatite (nHAp) has long been used as a biocompatible material for bone repair, bone regeneration, and bone reconstruction due to its low toxicity to local or systemic tissues. Various cross-linkers have been employed to maintain the structure of collagen; these include epigallocatechin-3-gallate (EGCG), which can fortify the mechanical properties of collagen and withstand the degradation of collagenase. We hypothesized that EGCG combined with nHAp may promote resin-dentin bonding durability. Here, we examined the effect of epigallocatechin-3-gallate-encapsulated nanohydroxyapatite/mesoporous silica (EGCG@nHAp@MSN) on thermal stability and remineralization capability of dentin collagen. Dentin slices (2 × 2 × 1 mm3 ) were obtained and completely demineralized in a 10% phosphoric acid water solution. The resulting dentin collagen matrix was incubated with deionized water, EGCG, nHAp@MSN, and EGCG@nHAp@MSN. The collagen thermal degradation temperature was assessed utilizing differential scanning calorimetry analysis, which indicated that EGCG, nHAp@MSN, and EGCG@nHAp@MSN reinforced collagen's capability to resist thermal degradation. EGCG@nHAp@MSN resulted in the highest increase in denaturation temperature. Thermogravimetric analysis showed that both nHAp@MSN and EGCG@nHAp@MSN achieved a higher residual mass than the EGCG and control groups. Fourier transform infrared spectroscopy was performed to examine the interaction between EGCG@nHAp@MSN and dentin collagen. The EGCG@nHAp@MSN sample exhibited stronger dentin microhardness and uppermost bond strength after thermocycling. EGCG significantly enhanced collagen's capability to resist thermal degradation. In summary, EGCG and nHAp@MSN may work together to assist the exposed collagen to improve resistance to thermal cycling and promote remineralization while also strengthening the durability of resin-dentin bonds.

Role of Natural Cross Linkers in Resin-Dentin Bond Durability: A Systematic Review and Meta-Analysis.

Background: The role of endogenous Matrix Metallo Proteinases in resin dentin bond deterioration over time has been well documented. The present study aimed to systematically review the literature; in vitro and ex vivo studies that assessed the outcomes of natural cross-linkers for immediate and long-term tensile bond strength were included. Methods: The manuscript search was carried out in six electronic databases—PubMed/MEDLINE, LILACS, SciELO, Cochrane, Web of Science and DOAJ, without publication year limits. Only manuscripts in English (including the translated articles) were selected, and the last search was performed in December 2020. The Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement was followed. Results: From the 128 potentially eligible studies, 48 full-text articles were assessed for eligibility. After eligibility assessment and exclusions, 14 studies were considered for systematic review and seven studies for meta-analysis. Amongst the selected studies for meta-analysis, three had a medium and four had a low risk of bias. Conclusions: It was evidenced by the available data that Proanthocyanidin is the most efficient natural cross-linker to date, in preserving the bond strength even after ageing.

Retention of Intrafibrillar Minerals Improves Resin–Dentin Bond Durability

The concept of extrafibrillar demineralization involves selective removal of apatite crystallites from the extrafibrillar spaces of mineralized dentin without disturbing the intrafibrillar minerals within collagen. This helps avoiding activation of endogenous proteases and enables air-drying of partially demineralized dentin without causing collapse of completely demineralized collagen matrix that adversely affects resin infiltration. The objective of the present study was to evaluate the potential of quaternized carboxymethyl chitosan (QCMC)–based extrafibrillar demineralization in improving resin–dentin bond durability. Isothermal titration calorimetry indicated that QCMC synthesized by quaternization of O-carboxymethyl chitosan had moderate affinity for Ca2+ (binding constant: 8.9 × 104 M−1). Wet and dry bonding with the QCMC-based demineralization produced tensile bond strengths equivalent to the phosphoric acid (H3PO4)–based etch-and-rinse technique. Those bond strengths were maintained after thermocycling. Amide I and PO43− mappings of QCMC-conditioned dentin were performed with atomic force microscope–infrared spectroscopy (AFM-IR). Whereas H3PO4-etched dentin exhibited an extensive reduction in PO43− signals corresponding to apatite depletion, QCMC-conditioned dentin showed scattered dark areas and bright PO43− streak signals. The latter were consistent with areas identified as collagen fibrils in the amide I mapping and were suggestive of the presence of intrafibrillar minerals in QCMC-conditioned dentin. Young’s modulus mapping of QCMC-demineralized dentin obtained by AFM-based amplitude modulation–frequency modulation recorded moduli that were the same order of magnitude as those in mineralized dentin and at least 1 order higher than H3PO4-etched dentin. In situ zymography of the gelatinolytic activity within hybrid layers created with QCMC conditioning revealed extremely low signals before and after thermocycling, compared with H3PO4-etched dentin for both wet and dry bonding. Confocal laser scanning microscopy identified the antibacterial potential of QCMC against Streptococcus mutans and Enterococcus faecalis biofilms. Taken together, the QCMC-based demineralization retains intrafibrillar minerals, preserves the elastic modulus of collagen fibrils, reduces endogenous proteolytic activity, and inhibits bacteria biofilms to extend dentin bond durability.

Effect of grape seed extract on the bond strength and adhesion durability of universal adhesive to dentin

The effects of grape seed extract (GSE) on the microtensile bond strength (µTBS) and resin-dentin bond durability of universal adhesive to dentin were investigated, while the resin-dentin interface was evaluated using a scanning electron microscope (SEM). Flat occlusal dentin surfaces of 80 sound human molars assigned to, control and GSE-pretreated groups & further divided into etch-and-rinse (E&R) and self-etch (SE) adhesion subgroups. In each subgroup, molars tested immediately (t0; n = 10) & after 6 months (t6; n = 10). In GSE-pretreated group, 10% GSE was prepared in hot water & then used for 1 min pretreatment after etching (15s) in the E&R subgroup or before application of adhesion in the SE subgroup. Composite build-ups made incrementally, and dentin beams sectioned and tested for µTBS at the baseline (24 h) and 6 months. Sections were obtained for SEM analysis. In the 6-month treatment, beams stored in artificial saliva, subjected to thermal cycling, and then tested. The failure modes of fracture surfaces were identified with a stereomicroscope. Comparing the t0 and t6 timepoints, significant reduction in µTBS in all groups compared with their respective controls after aging, but the percentage of bond loss due to aging in the GSE-pretreated groups was less than controls. Most failure modes were mixed. GSE (10%) pretreatment for 1 min before adhesion enhanced the durability of the resin-dentin bond but did not completely eliminate loss of bond strength.

Effect of Cavity Cleanser With Long-Term Antibacterial and Anti-Proteolytic Activities on Resin-Dentin Bond Stability.

ObjectiveSecondary caries caused by oral microbiome dysbiosis and hybrid layer degradation are two important contributors to the poor resin–dentin bond durability. Cavity cleansers with long-term antimicrobial and anti-proteolytic activities are in demand for eliminating bacteria-induced secondary caries and preventing hybrid layers from degradation. The objectives of the present study were to examine the long-term antimicrobial effect and anti-proteolytic potential of poly(amidoamine) dendrimers with amino terminal groups (PAMAM-NH2) cavity cleanser.MethodsAdsorption tests by attenuated total reflectance–infrared (ATR-IR) spectroscopy and confocal laser scanning microscopy (CLSM) were first performed to evaluate whether the PAMAM-NH2 cavity cleanser had binding capacity to dentin surface to fulfill its relatively long-term antimicrobial and anti-proteolytic effects. For antibacterial testing, Streptococcus mutans, Actinomyces naeslundii, and Enterococcus faecalis were grown on dentin surfaces, prior to the application of cavity cleanser. Colony-forming unit (CFU) counts and live/dead bacterial staining were performed to assess antibacterial effects. Gelatinolytic activity within the hybrid layers was directly detected by in situ zymography. Adhesive permeability of bonded interface and microtensile bond strength were employed to assess whether the PAMAM-NH2 cavity cleanser adversely affected resin–dentin bonding. Finally, the cytotoxicity of PAMAM-NH2 was evaluated by the Cell Counting Kit-8 (CCK-8) assay.ResultsAdsorption tests demonstrated that the binding capacity of PAMAM-NH2 on dentin surface was much stronger than that of 2% chlorhexidine (CHX) because its binding was strong enough to resist phosphate-buffered saline (PBS) washing. Antibacterial testing indicated that PAMAM-NH2 significantly inhibited bacteria grown on the dentin discs as compared with the control group (p < 0.05), which was comparable with the antibacterial activity of 2% CHX (p > 0.05). Hybrid layers conditioned with PAMAM-NH2 showed significant decrease in gelatin activity as compared with the control group. Furthermore, PAMAM-NH2 pretreatment did not adversely affect resin–dentin bonding because it did not decrease adhesive permeability and microtensile strength. CCK-8 assay showed that PAMAM-NH2 had low cytotoxicity on human dental pulp cells (HDPCs) and L929.ConclusionsPAMAM-NH2 cavity cleanser developed in this study could provide simultaneous long-term antimicrobial and anti-proteolytic activities for eliminating secondary caries that result from a dysbiosis in the oral microbiome and for preventing hybrid layers from degradation due to its good binding capacity to dentin collagen matrix, which are crucial for the maintenance of resin–dentin bond durability.

Effect of Dimethyl Sulfoxide wet bonding technique on the durability of resin-dentin bond of an etch-and rinse adhesive system under in-vivo simulating conditions

Purpose: This study assessed the influence of new dimethyl sulfoxide (DMSO) containing pretreatments on the aged resin-dentin micro-tensile bond strength (μTBS) of a two-step etch-and rinse adhesive system. Methods: Occlusal enamel of forty-eight sound molar teeth were ground to expose mid-coronal dentin. Prepared specimens were restored with AdperTM Single Bond2 (SB2) adhesive systems. Prior to the adhesive system application, specimens were randomly assigned into three groups according to dentin pretreatment [no treatment (control group), ethanol wet-bonding (EtOH), or 50 vol% DMSO dissolved in ethanol (DMSO/EtOH)]. Resin composite block was build-up for each specimen. Specimens were stored under simulated IPP 20 mmHg, immersed in artificial saliva at 37 oC for either 24h or 6m. Specimens were sectioned into sticks of 1mm2 for μTBS testing using a universal testing machine. Mode of failure was analyzed using a scanning electron microscope. Three extra sticks from each subgroup were prepared and assessed for the amount of silver nitrate penetration (wt%) using energy dispersive analytical x-ray (EDAX). Data were statistically analyzed by Two-way ANOVA of Variance. Results: (DMSO/EtOH) group revealed the highest statistically significant mean bond strength and the least silver nitrate penetration compared to the (control) and (EtOH) groups at 24h and 6m. Adhesive failures was most predominant for control group at 24h, with increased percentage of mixed failure for EtOH and DMSO/EtOH groups at 24h and 6m. Conclusions: DMSO/EtOH dentin pretreatment improved the immediate and delayed resin-dentin bond.

Enhancing resin-dentin bond durability using a novel mussel-inspired monomer.

Numerous approaches have been developed to improve the resin-dentin bond performance, among which the bio-application of mussel-derived compounds have drawn great attention recently. To assess the performance of N-(3,4-dihydroxyphenethyl)methacrylamide (DMA), a mussel-derived compound, as a functional monomer in dental adhesive, its potential property to cross-link with dentin collagen and polymerize with adhesive will first be evaluated by transmission electron microscopy (TEM), attenuated total reflectance technique of Fourier transform infrared (ATR-FTIR), and atomic force microscopy (AFM) via Peakforce QNM mode. After validating the influence of DMA on collagen and adhesive separately, the overall performance of DMA/ethanol solution as a primer in dentin bonding was examined using micro-tensile bond strength (μTBS) testing, fracture pattern observation, and nanoleakage evaluation both immediately and after 10,000 times thermocycling aging. The inhibitory effect of DMA on endogenous metalloproteinases (MMPs) was evaluated by in situ zymography using confocal laser scanning microscopy (CLSM) and the cytotoxicity of DMA was evaluated using cell counting kit-8. Results demonstrated that DMA successfully cross-linked with dentin collagen via non-covalent bonds and had no influence on the polymerization and mechanical properties of the adhesive. Furthermore, even after 10,000 times thermocycling aging, the μTBS and nanoleakage expression of the DMA-treated groups showed no significant change compared with their immediate values. In situ zymography revealed reduced endogenous proteolytic activities after the application of DMA, and no cytotoxicity effect was observed for DMA concentration up to 25 ​μmol/L. Thus, DMA could be used as a novel, biocompatible functional monomer in dentin bonding.

Synergistic effects of graphene quantum dots and carbodiimide in promoting resin–dentin bond durability

ObjectiveResin-based dental adhesion is mostly utilized in minimally invasive operative dentistry. However, improving the durability and stability of resin–dentin bond interfaces remain a challenge. Graphene quantum dots (GQDs) reinforced by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) were introduced to modify the resin–dentin bond interfaces, thereby promoting their durability and stability. MethodsGQDs, EDC, and EDC+GQDs groups were designed to evaluate the effects of GQDs and EDC on collagenase activity, the interaction of GQDs with collagen, and the resin–dentin interface. First, the effects of GQDs and EDC on collagenase activity was evaluated by Collagenase (EC 3.4.24.3) reacting with its substrate. The interaction of GQDs and EDC with collagen were evaluated by cross-linking degree analysis, sodium dodecyl sulfate–polyacrylamide gel electrophoresis, attenuated total reflection Fourier transform infrared spectroscopy and enzymatic hydrolysis. Second, the acid-etched and rinse adhesive system was used to evaluate the resin–dentin bond on the basis of microtensile bond strength, in situ zymography and fluorescence confocal laser scanning microscopy. ResultsGQDs could inhibit collagenase activity. GQDs with the aid of EDC could cross-link collagen via covalent bonds and improve the anti-enzymatic hydrolysis of collagen. In the resin–dentin adhesion model, the μTBS of the EDC+GQDs group was significantly higher than the other control groups after thermocycling. The addition of EDC to GQDs could inhibit matrix metalloproteinase activity and promote the integrity of the bonding interfaces after thermocycling. SignificanceThis study presents a novel strategy to modify the resin–dentin interface and provides a new application for GQDs. This strategy has the potential to improve the durability of resin-based restoration in dentistry.

The pursuit of resin-dentin bond durability: Simultaneous enhancement of collagen structure and polymer network formation in hybrid layers

ObjectiveImperfect polymer formation as well as collagen’s susceptibility to enzymatic degradation increase the vulnerability of hybrid layers over time. This study investigated the effect of new dimethyl sulfoxide (DMSO)-containing pretreatments on long-term bond strength, hybrid layer quality, monomer conversion and collagen structure. MethodsH3PO4-etched mid-coronal dentin surfaces from extracted human molars (n = 8) were randomly treated with aqueous and ethanolic DMSO solutions or following the ethanol-wet bonding technique. Dentin bonding was performed with a three-step etch-and-rinse adhesive. Resin-dentin beams (0.8 mm2) were stored in artificial saliva at 37 °C for 24 h and 2.5 years, submitted to microtensile bond strength testing at 0.5 mm/min and semi-quantitative SEM nanoleakage analysis (n = 8). Micro-Raman spectroscopy was used to determine the degree of conversion at different depths in the hybrid layer (n = 6). Changes in the apparent modulus of elasticity of demineralized collagen beams measuring 0.5 × 1.7 × 7 mm (n = 10) and loss of dry mass (n = 10) after 30 days were calculated via three-point bending and precision weighing, respectively. ResultsDMSO-containing pretreatments produced higher bond strengths, which did not change significantly over time presenting lower incidence of water-filled zones. Higher uniformity in monomer conversion across the hybrid layer occurred for all pretreatments. DMSO-induced collagen stiffening was reversible in water, but with lower peptide solubilization. SignificanceImproved polymer formation and higher stability of the collagen-structure can be attributed to DMSO’s unique ability to simultaneously modify both biological and resin components within the hybrid layer. Pretreatments composed of DMSO/ethanol may be a viable-effective alternative to extend the longevity of resin-dentin bonds.

Effect of dentin biomodification techniques on the stability of the bonded interface.

Aim:The aim of this study is to evaluate the effect of different bonding techniques ethanol wet bonding and dimethyl sulfoxide (DMSO) wet bonding and a novel collagen cross-linker Quercetin application on the durability of resin-dentin bond and observe the bonded interface under the scanning electron microscope (SEM).Materials and Methods:For shear bond strength testing, flat coronal dentin surfaces were prepared on 110 extracted human molars. Teeth were randomly divided into five experimental groups according to different surface pretreatments techniques. Group A was control group without any surface pretreatment. In Group B, ethanol wet bonding pretreatment was done before the application of adhesive. In Group C, DMSO wet bonding was done before the application of adhesive and in Groups D and E, Quercetin along with ethanol and Quercetin along with DMSO pretreatment, respectively, were done before adhesive application. Composite restorations were placed in all the samples. Twenty samples from each group were subjected to immediate and delayed (9 months) shear bond strength evaluation. In addition, two samples per group were subjected to the scanning electron microscopic analysis for the observation of resin-dentin interface.Statistical Analysis:Data collected were subjected to the statistical analysis using the one-way analysis of variance and post hoc Tukey's test at a significance level of P < 0.05.Results:Dentin pretreatment with all the techniques resulted in significantly higher resin-dentin bond strength after 9 months storage with DMSO group showing the highest bond strength values.Conclusion:It can be concluded that these biomodification techniques can improve the durability of the resin-dentin bond.

Effect of remineralizing agents on resin-dentin bond durability of adhesive restorations: An in vitro study

Aim: To prolong the clinical success and durability of bonded restorations, hybrid layer stabilization is crucial for better adhesion between the resin and tooth substrate. The aim of this in vitro study was to assess the ability of self-assembling peptide (curodont protect) and a remineralizing agent (MI paste plus) in promoting biomimetic remineralization for stabilization of the hybrid layer to improve the resin-dentin bond durability. Materials and Methods: Standardized mesio-occlusal (MO) and disto-occlusal (DO) cavities were prepared on 60 mandibular molars. Teeth were randomly allocated to different groups and after acid etching, half of the MO cavities (n = 30) of dentin were treated with curodont protect and another half (n = 30) with MI paste plus. Half of the DO cavities (n = 30) were pretreated with chlorhexidine, whereas the other half did not receive any dentin pretreatment before placing adhesive restoration. Half of the samples from each group (n = 15) were subjected to thermomechanical load cycles. Immediate and after aging bond strengths were estimated using a universal testing machine, and the type of bond failure was assessed under a scanning electron microscope. Data obtained in megapascals were analyzed by one-way analysis of variance and Tukey’s multiple post hoc test with the significance level set as P ≤ 0.05. Results: Immediate bond strength values were significantly high for the control group and low for the MI paste plus group (P = 0.002). Significant differences were not observed between the curodont protect and chlorhexidine (P = 0.0514) treated groups. In all groups, bond strength values were decreased significantly after thermomechanical cyclic (TMC) loading (P = 0.0001). Adhesive type fractures were reported more in failure mode analysis. Conclusion: All groups exhibited reduced microtensile bond strength values after aging. The dentin treated with chlorhexidine, curodont protect, and MI paste plus produced more durable bonding than the control group.

Assessment of Hydroxyapatite Nanospheres Incorporated Dentin Adhesive. A SEM/EDX, Micro-Raman, Microtensile and Micro-Indentation Study

Hydroxyapatite (HA) delivery with resin adhesives has potential for re-mineralization of resin–dentin interface. The study prepared an adhesive containing HA and confirmed its presence in adhesive and interaction with the dentin using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and Micro-Raman spectroscopy. The aim was to assess the influence of HA incorporation in dentin adhesive on its microtensile bond strength (μ-tbs) and Knoop microhardness (KHN). Thirty teeth each were bonded with CA and HA adhesive using a 10-s smear and photo-polymerized. The specimens in each adhesive group (CA and HA) were divided into sub-groups of 24 h, 8 weeks, and 16 weeks (n = 10) aging durations. μ-tbs was assessed at a crosshead speed of 0.5 mm/minute and bonded interface was analyzed using SEM (n = 20) and Raman spectroscopy (n = 10). Softening of HA adhesive and CA was assessed using KHN. HA adhesive presented higher μ-tbs compared to CA. With an increase in storage time, HA adhesive presented with 100% adhesive failure. Softening was less and KHN was higher for HA adhesive compared to CA (p &lt; 0.05). KHN reduction was higher in CA [19.6 (5.1)%] compared to the HA adhesives [9.7 (4.5)%]. HA adhesive showed superior μTBS and microhardness compared to CA. In the absence of nanoleakage, HA modified adhesive exhibited enhanced bond integrity and better durability of resin dentin bond compared to control adhesive.

Thermal and bioactive optimization of a unidose 3-step etch-and-rinse dentin adhesive

Statement of problemThe limited durability of resin-dentin bonds is considered a major disadvantage of adhesive restorations. Therefore, clinical strategies have been developed to improve hybrid layer stability over time. These strategies require testing. PurposeThe purpose of this in vitro study was to evaluate the influence of preheating and the inclusion of a bioactive glass in a unidose 3-step etch-and-rinse adhesive system on the adhesion of direct composite resin restorations. Material and methodsDentin disks from 80 molars were assigned to 8 groups (n=10): CG-T1/CG-T2: control group; PG-T1/PG-T2: adhesive preheated to 68 °C; BG-T1/BG-T2: 0.05 mg of Bioglass 45S5 (BAG) (particle size: 3 μm) added to primer; PBG-T1/PBG-T2: adhesive and BAG-modified primer preheated to 68 °C. Sticks were fabricated for microtensile bond strength (μTBS) testing and were tested at 1 week (T1) and after 6 months (T2) of storage. μTBS data were analyzed by using 2-way ANOVA and the Tukey-Kramer post hoc test (α=.05). Scanning electron microscopy was used to analyze the failure mode. Attenuated total reflection Fourier transform infrared spectroscopy was used to quantitatively analyze the modifications to the chemical structure of the adhesive system from preheating and BAG inclusion. ResultsThe mean bond strength values at 1 week were statistically different, with PG-T1 (69.8 ±7.8 MPa) superior to all other groups. CG-T1 (58.2 ±6.7 MPa), BG-T1 (60.7 ±4.4 MPa), and PBG-T1 (61.0 ±4.6 MPa) were not statistically different (P>.05). PG-T2 maintained the highest bond strength at 6 months (68.3 ±3.7 MPa), with no decrease in μTBS observed over time. Failure modes were mostly adhesive. Attenuated total reflection Fourier transform infrared spectroscopy analysis reported that primer preheating caused solvent evaporation and revealed that preheating the bonding agent promoted the condensation reaction between the silane and adhesive fillers. ConclusionsNo decrease in μTBS was observed for any group after 6 months. Preheating the adhesive system (primer and bonding resin) significantly increased the 1-week and 6-month μTBS. Inclusion of BAG did not affect the bond strength.

The application of mussel-inspired molecule in dentin bonding

ObjectivesTo evaluate the effect of dopamine methacrylamide (DMA) pretreatment on resin-dentin bond durability. MethodsForty acid-etched dentin surfaces were randomly allocated into five groups, and pretreated with one of the primers: 0.1, 1.0, and 10 mmol/L DMA/ dimethyl sulfoxide (DMSO) solutions, pure DMSO, and deionized water (control) before bonding. The bonded teeth of each group were randomly distributed into an immediate subgroup (water storage at 37 °C for 24 h) and an aged subgroup (thermocycling, 5−55 °C, 10,000 times). The teeth were sectioned into 0.9 mm slabs; two middle slabs were used for nanoleakage evaluation, while the remaining slabs were sectioned into beams for micro-tensile bond strength (MTBS) test. Fractured mode and nanoleakage were observed under SEM. FTIR was used to assess the effect of primers on the degree of conversion (DC) of adhesive. ResultsThe MTBS was significantly affected by primers (p < 0.001), aging conditions (p < 0.001) and their interactions (p < 0.001) as revealed by two-way ANOVA. Significant increase in nanoleakage was observed in both the aged control and DMSO groups when compared to their immediate values (p < 0.05); while no differences were observed in the aged DMA-treated groups (p > 0.05). Among the three DMA-treated groups, 1.0 mM DMA/DMSO primer manifested the highest MTBS with the lowest nanoleakage after thermocycling aging. Application of 10 mM DMA/DMSO significantly decreased the DC of adhesive (p < 0.05). ConclusionsThe 1.0 mM DMA/DMSO solution is a potential functional primer to enhance the durability of resin-dentin bond. Clinical significanceSixty seconds pretreatment of 1.0 mM DMA/DMSO solution on dentin surfaces provides dentists an alternative choice to preserve dentin bond strength. Dopamine methacrylamide might strengthen the integrity of hybrid layer by serving as a bridge connecting the demineralized collagen fibrils and adhesive polymer network.

Improving Resin-dentin Bond Durability and a Novel Ultra-high-speed Videography

Improving Resin-dentin Bond Durability and a Novel Ultra-high-speed Videography

Effect of polyvinylphosphonic acid on resin-dentin bonds and the cytotoxicity of mouse dental papilla cell-23

Statement of problemPolyvinylphosphonic acid (PVPA) could be used as a biomimetic remineralization analog and a matrix metalloproteinases (MMPs) inhibitor. However, studies are lacking regarding the performance of PVPA in dental bonding systems for maintaining the durability of the resin-dentin bond. PurposeThe purpose of this in vitro study was to investigate the effect of PVPA on the durability of resin-dentin bonds and the viability of mouse dental papilla cell-23 (MDPC-23). The mechanical properties of resin-dentin interfaces during long-term storage were analyzed, and the potential application of PVPA as a biomimetic remineralization analog in adhesive dentistry was evaluated. Material and methodsSeventy-five extracted noncarious human third molars were collected and randomly divided into 5 groups, and then the microtensile bond strength (μTBS) data and scanning electron microscope (SEM) images were used to evaluate the preservation condition of resin-dentin bonds after 1 day, 6 months, and 1 year of storage. The cytotoxicity of PVPA was detected by cell proliferation assay and cell apoptosis assay. ResultsCompared with the control and chlorhexidine (CHX) groups, the combined group (treated with both 200-μg/mL PVPA and biomimetic remineralization) had excellent bond durability. The exposed collagen fibril from the PVPA-treated groups (included 200-μg/mL and 500-μg/mL PVPA groups and a combined group) still showed integrity after 1 year of storage when compared with the control group. PVPA up to 500 μg/mL showed no cytotoxicity to MDPC-23 and did not inhibit cell growth. ConclusionsThis study offered evidence that PVPA did not result in cytotoxicity at low concentrations as an MMP inhibitor and a biomimetic remineralization analog. In addition, the application of PVPA improved bond strength and preserved collagen integrity after 1 year of in vitro storage.

New silyl-functionalized BisGMA provides autonomous strengthening without leaching for dental adhesives

Resin-based composite has overtaken dental amalgam as the most popular material for direct restorative dentistry. In spite of this popularity the clinical lifetime of composite restorations is threatened by recurrent decay. Degradation of the adhesive leads to gaps at the composite/tooth interface–bacteria, bacterial by-products and fluids infiltrate the gaps leading to recurrent decay and composite restoration failure. The durability of resin-dentin bonds is a major problem. We address this problem by synthesizing silyl-functionalized BisGMA (e.g., silyl-BisGMA), formulating dental adhesives with the new monomer and determining the physicochemical properties and leaching characteristics of the silyl-BisGMA adhesives. Silyl-BisGMA was synthesized by stoichiometric amounts of BisGMA and 3-isocyanatopropyl trimethoxysilane (IPTMS). The control adhesive was a mixture based on HEMA/BisGMA (45/55, w/w). In the experimental formulations, BisGMA was partially or completely replaced by silyl-BisGMA. Water miscibility, polymerization behavior (Fourier transform infrared spectroscopy, FTIR), thermal property (modulated differential scanning calorimetry, MDSC), mechanical properties in dry and wet conditions (dynamic mechanical analysis, DMA), and leached species (HPLC) were investigated. Data from all tests were submitted to appropriate statistical analysis (α = 0.05). Silyl-BisGMA-containing adhesives exhibited comparable water miscibility, lower viscosities, and significantly improved degree of conversion of CC bond as compared to the control. After 4 weeks aqueous aging, the glass transition temperature and rubbery moduli of the experimental copolymers were significantly greater than the control (p < 0.05). HPLC results indicated a substantial reduction of leached HEMA (up to 99 wt%) and BisGMA (up to 90 wt%). By introducing silyl-functional group, the new BisGMA derivative exhibited potential as a monomer that can lead to dental adhesives with improved mechanical properties and reduced leaching under conditions relevant to the oral environment. Statement of SignificanceThe low-viscosity adhesive that bonds the composite to the tooth (enamel and dentin) is intended to seal and stabilize the composite/tooth interface, but it degrades leading to a breach at the composite/tooth margin. As the most popular crosslinking monomer in adhesives, Bisphenol A-glycerolate dimethacrylate (BisGMA) has limitations, e.g. susceptible to hydrolysis and concomitant property degradation. A methoxysilyl-functionalized BisGMA derivative (silyl-BisGMA) was introduced in this work to respond to these limitations. Our results indicated that by introducing silyl-BisGMA, higher crosslinked networks were obtained without sacrificing the homogeneity, and the leached amount of HEMA was reduced up to 99%. This novel resin offers potential benefits including prolonging the functional lifetime of dental resin materials.

Chitosan-Based Extrafibrillar Demineralization for Dentin Bonding

Instability of resin-dentin bonds is the Achilles’ heel of adhesive dentistry. To address this problem, a chelate-and-rinse extrafibrillar dentin demineralization strategy has been developed that keeps intrafibrillar minerals within collagen fibrils intact to prevent activation of endogenous proteases that are responsible for collagen degradation within hybrid layers. The objective of the present study was to evaluate the potential of using chitosan >40 kDa as an antimicrobial extrafibrillar dentin-chelating agent to enhance bond durability. Transmission electron microscopy provided evidence for retention of intrafibrillar minerals and smear plugs in dentin conditioned with 1 wt% chitosan. Analyzed by Kruskal-Wallis analysis of variance, Dunn’s statistic, and separate Mann-Whitney tests, tensile bond strengths to wet- and dry-bonded dentin indicated that chelating dentin with chitosan for 60 s prior to bonding did not result in a significant decline in resin-dentin bond strength when compared with that of phosphoric acid etching (P > 0.05). Gelatinolytic activity within the hybrid layers was examined via in situ zymography after 24-h storage or after thermomechanical cycling and analyzed with 3-factor analysis of variance. After 24 h, enzymatic activity was detected only within completely demineralized phosphoric acid–etched dentin, with values derived from dry bonding significantly higher than those derived from wet bonding (P < 0.05). Negligible fluorescence was detected within hybrid layers when dentin was conditioned with chitosan, even after thermomechanical cycling, as compared with the controls. Reduction in water permeability in chitosan-conditioned dentin, attributed to smear plug retention, also fostered long-term bond stability. Antibacterial testing performed with live/dead staining indicated that the acetic acid–solubilized chitosan possessed antibacterial activities against 3 single-species biofilms: Streptococcus mutans, Actinomyces naeslundii, and Enterococcus faecalis. Taken together, the new chitosan-based extrafibrillar demineralization strategy retains intrafibrillar minerals, reduces endogenous protease-initiated collagen degradation, prevents water permeation within hybrid layers, and kills bacteria on dentin surfaces, which are crucial factors for enhancing resin-dentin bond durability.