Residual Peroxide Research Articles

Catalytic Decomposition of Residual Hydrogen Peroxide in N-Methylmorpholine-N-oxide by Heteroatom Doping Modified Activated Carbon

N-methyloxymoroline-N-oxide (NMMO) has been used in large quantities for the production of new solvent-treated cellulose. But, excess H2O2 used in the synthesis of NMMO from N-methylmorpholine (NMM) can adversely affect the quality of the product. This study introduces a novel approach using nitrogen and sulfur co-doped activated carbon (N,S co-doped AC) to catalyze the decomposition of residual H2O2 in NMMO products. Characterization techniques including N2 adsorption/desorption, Raman, TEM, FT-IR, and XPS were used to study the surface properties and chemistry of activated carbon doped with heteroatoms. The results revealed that AC800NS800 (800 is the calcinate temperature, NS is denoted by the co-doped of N and S) exhibited superior catalytic performance for H2O2 decomposition compared to singly doped activated carbons. Notably, over 95% of H2O2 in NMMO was decomposed, which the catalytic efficiency of AC800NS800 is no significant loss after ten times reuse. The exceptional catalytic activity of AC800NS800 is attributed to the presence of various functional groups on the surface (graphitic carbon, graphitic nitrogen, and pyrrole nitrogen). EPR tests identified HO• and O2•− as the primary reactive species in the H2O2 decomposition process. This study provides valuable insights into the catalytic decomposition of H2O2 in NMMO solutions and offers a significant reference for the production of high-quality NMMO.

An improved method for measuring catalase activity in biological samples.

Catalase (CAT) is an important enzyme that protects biomolecules against oxidative damage by breaking down hydrogen peroxide (H2O2) into water and oxygen. CAT is present in all aerobic microbes, animals, and plants. It is, however, absent from normal human urine but can be detected in pathological urine. CAT testing can thus help to detect such urine. This study presents a novel spectrophotometric method for determining CAT activity characterized by its simplicity, sensitivity, specificity, and rapidity. The method involves incubating enzyme-containing samples with a carefully chosen concentration of H2O2 for a specified incubation period. Subsequently, a solution containing ferrous ammonium sulfate (FAS) and sulfosalicylic acid (SSA) is added to terminate the enzyme activity. A distinctive maroon-colored ferrisulfosalicylate complex is formed. The formation of this complex is a direct result of the reaction between FAS and any residual peroxide present. This leads to the generation of ferric ions when coordinated with SSA. The complex has a maximum absorbance of 490 nm. This advanced method eliminates the need for concentrated acids to stop CAT activity, making it safer and easier to handle. A comparative analysis against the standard ferrithiocyanate method showed a correlation coefficient of 0.99, demonstrating the new method's comparable effectiveness and reliability. In conclusion, a simple and reliable protocol for assessing CAT activity, which utilizes a cuvette or microplate, has been demonstrated in this study. This interference-free protocol can easily be used in research and clinical analysis with considerable accuracy and precision.

Comparative understanding of peroxide quantitation assays: a case study with peptide drug product degradation.

Peroxide-mediated oxidation of drug molecules is a known challenge faced throughout the pharmaceutical development pathway-from early-stage stability studies to manufacturing processes. During the initial development stage, the major source of peroxide is the formulation excipients, whether they are pre-loaded or generated in situ due to slow degradation, and in the late phase, peroxides can be introduced during sanitization processes or generated via cavitation. In essence, a control strategy for peroxide mitigation often becomes a critical quality attribute for successful drug development. To this end, quantitation of peroxide is essential to monitor the peroxide level to ensure product quality and proposed shelf-life. However, methods for reliable and robust quantitation to detect trace levels of peroxide in a complex drug product matrix become increasingly challenging. This article discusses three high-throughput assays based on absorbance, fluorescence and chemiluminescence measurements to detect peroxide at a low level and compares the methods through validation studies in water. Selected methods have also been tested to understand the forced degradation of model peptide drug products with spiked hydrogen peroxide. Peptide degradation profiles and residual peroxide levels are presented to provide an understanding of the suitability of the quantitation methods and their performance.

Effect of bleaching treatments on the adhesion of orthodontic brackets: a systematic review

BackgroundNowadays bleaching procedures have gained popularity in orthodontic patients. Peroxide and Carbamide acids are the common agents which are used in in-office and at home bleaching techniques.Consequently, the Bonding adhesion to the enamel can be influenced by the orthodontic phase and the residual peroxide might interfere with the polymerization and the adhesion of the brackets.Frequent debonding of the brackets from teeth after the bleaching procedure could cause the lengthening of the therapy and promote irregularities on enamel surface derived from an additional bonding phase of the brackets.The aim of this systematic review is to appraise the influence regarding the effect of the bleaching procedure on the bond strength of orthodontic brackets.MethodsAn electronic database search was performed. Search terms included: bleaching, brackets, adhesion; data were extracted and summarized. Risk of bias was assessed using the Chocrane risk of bias tool, adapted for in vitro studies.ResultsA total of 8689 articles were screened and 11 studies met the inclusion criteria of this systematic review.1000 teeth of human and bovine origin were analyzed for the shear bond strength (SBS) of stainless and ceramic brackets after the bleaching treatments. All the authors divided the groups in different subgroups with different bleaching agents and in different concentration.The SBS value allowed to demonstrate the necessity to delay the bonding of the brackets for two weeks after a bleaching treatment and its improvement when tooth mousse or antioxidants agents are used.ConclusionsThe SBS values and the delay of the bonding procedure must be considered in dental practice and clinical strategies are necessary in order to avoid drawbacks which could cause the debonding of the brackets after bleaching due to the alterations of the dental substrate, thus interfering with the orthodontic treatments.

Sodyum borhidrürün Pinus brutia Ten ve buğday sapı hamurlarının peroksit ağartması üzerindeki etkileri

The addition of sodium borohydride (NaBH4) to peroxide bleaching has been investigated for its effects on the bleaching yield, mechanical and optical properties of Pinus brutia and wheat straw pulps. The pulps were subjected to four different bleaching conditions by adding charges of NaBH4 at 0%, 0.5%, 1.0%, and 1.5%, while keeping a constant hydrogen peroxide charge of 7%. The use of NaBH4 in the peroxide bleaching processes of the two pulps increased the bleaching yields. The results show that the addition of NaBH4 significantly improved the mechanical properties of the pulps, including tensile and burst indices. The optical properties of the pulps were also improved with the addition of NaBH4. The increase in mechanical and optical properties can be attributed to the reduction of residual hydrogen peroxide and the removal of metal ions, while the decrease in yellowness is due to the removal of metal ions that can cause oxidative degradation of the pulp. The findings of this study suggest that the addition of NaBH4 as an additive in peroxide bleaching is a promising approach to improve the mechanical and optical properties of P. brutia and wheat straw pulps, which can be further explored in future research.

Inactivation of Salmonella enterica in black peppercorn by fluidization with hydrogen peroxide vapor

Salmonella is the most common bacterial pathogen associated with product recalls and outbreaks in spices. Spices are in the top three food categories for greatest number of recalls due to microbiological contamination. Current validated microbial reduction techniques for spices are tied to human health and environment concerns or negatively affect the quality characteristics of the spice which has led to the emerging of alternative technologies such as hydrogen peroxide vapor (HPV). hydrogen peroxide vapor treatment was conducted at two different temperatures (45°C and 60°C) and two dwell times (30 and 60 min). Microbial reduction and residual hydrogen peroxide were measured at three storage times: 0 h (immediately after treatment), 24 and 48 h post-treatment. The effect of HPV on the quality of whole black peppercorn was evaluated 48 h post-treatment based on changes in piperine content, total phenolics, antioxidant activity, total volatile compounds, and color. Reduction in Salmonella population ranged from 1.41–2.83 log CFU/g. Residual hydrogen peroxide of up to 500 ppm was still detected on samples after 48 h of storage. All quality parameters except for color remained unaffected between treated and untreated whole black peppercorn. The study highlights the need to explore further process design modifications before conclusions can be made on the efficacy of HPV treatment as a means for low-moisture foods pasteurization.

Colorimetric and fluorescent dual-signals probes for naked-eye detection of hydrogen peroxide and applications in milk samples and in vivo

Excessive residual hydrogen peroxide (H2O2) disinfectant in food is harmful to human health. Therefore, it is necessary to develop efficient detection methods for H2O2 detection. In this work, we designed and synthesized five D-A molecules 3a-3e by introducing electron-donor substituents (–OCH3 and –CH3) to the electron-acceptor dicyanoisophorone skeleton in order to find out the suitable probes for H2O2 detection. Among them, two promising probes, 3a and 3c, are screened out according to structure-property relationships. Based on the principle of intramolecular charge transfer (ICT), 3a and 3c express colorimetric and fluorescent dual-signals towards H2O2 with low detection limits (0.20 μM and 0.14 μM) and rapid response (within 20 mins). The reaction mechanism between probes and H2O2 is determined by 1H NMR and HRMS. Density functional theory (DFT) calculations are measured to study the regulation mechanism of structure adjustment on probs performance. Furthermore, a smartphone RGB analysis is utilized as a portable platform for the quantitative detection of H2O2 without complicated instruments, indicating a high efficiency and on-site detection method for H2O2. In addition, probes are applied to detect H2O2 in milk samples, HepG-2 cells and zebrafish, suggesting the promising applications in food samples and physiological systems.

Effect of Residual Hydrogen Peroxide Content in Ammonia Hydrogen Peroxide Wastewater on Subcritical Hydrothermal Reaction

生物亜硝酸化と亜臨界水熱反応の組合せで，高濃度排窒素の消滅型除去ができることを我々は報告した．本稿では過酸化水素（H2O2）を含有する半導体の基盤洗浄水などのアンモニア過水に対する水素還元と水熱反応を併用した高濃度窒素除去を検討した．既報の方法を適用するためには，源排水からH2O2のみを水素還元除去する工程を前段に付与する必要があることを見出した．

Ageing tests closer to real service conditions using hyper-sensitive microcalorimetry, a case study on EPDM rubber

Accelerated thermal ageing (ATA) coupled to mechanical testing is widely used to predict the lifetime of polymeric products. ATA implies that the mechanisms of ageing are the same at accelerated and service conditions, which may often not be the case. Hence, ageing closer to service conditions is of high importance, but require very sensitive tools. Therefore, a high sensitivity microcalorimetry (MC) method was applied here to assess if it can be a possible tool for lifetime/ageing prediction closer to service conditions. We chose to focus on a complex, yet commonly used, ethylene-propylene-diene terpolymer (EPDM) rubber. Arrhenius extrapolation of the heat flow data indicated two regimes at low and high temperature, with the former having the lower activation energy. The heat flow values measured by the MC revealed contributions from processes such as the melting of the antioxidant, its consumption at low temperature and the breakdown of residual peroxide. MC tests on the EPDM indicated a very low degree of oxidation appearing above 100 °C, too low to be observed with infra-red spectroscopy (FTIR), but noticeable with MC. The high sensitivity of the MC techniques enabled detection of early signs of polymer degradation/ageing and other thermally activated processes that take place at or close to service temperatures (such as those in nuclear power plants). The MC tests were combined with other techniques, such as scanning electron microscopy/energy dispersive X-ray spectroscopy, gas chromatography techniques, differential scanning calorimetry and FTIR to further understand the degradation mechanisms.

Filtration performance, fit test and side effects of respiratory personal protective equipment following decontamination: Observations for user safety and comfort.

While facing personal protective equipment (PPE) shortages during the COVID-19 pandemic, several institutions looked to PPE decontamination and reuse options. This study documents the effect of two hydrogen peroxide treatments on filtration efficiency and fit tests as well as the side effects for volunteers after the decontamination of N95 filtering facepiece respirators (FFRs). We also propose an efficient and large-scale treatment protocol that allows for the traceability of this protective equipment in hospitals during PPE shortages. The effects of low-temperature hydrogen peroxide sterilization and hydrogen peroxide vapor (HPV) on two FFR models (filtration, decontamination level, residual emanation) were evaluated. Ten volunteers reported comfort issues and side effects after wearing 1h FFRs worn and decontaminated up to five times. The decontamination process does not negatively affect FFR efficiency, but repeated use and handling tend to lead to damage, limiting the number of times FFRs can be reused. Moreover, the recommended 24-h post-treatment aeration does not sufficiently eliminate residual hydrogen peroxide. Prolonged aeration time increased user comfort when using decontaminated FFRs. HPV and low-temperature hydrogen peroxide sterilization seem to be appropriate treatments for FFR decontamination when the PPE is reused by the same user. PPE decontamination and reuse methods should be carefully considered as they are critical for the comfort and safety of healthcare workers.

Decolorization and detoxication of plant-based proteins using hydrogen peroxide and catalase

The gap between the current supply of meat and its predicted future demand is widening, increasing the need to produce plant-based meat analogs. Despite ongoing technical developments, one of the unresolved challenges of plant-based meat analogs is to safely and effectively decolor plant proteins that originally exhibit yellow–brown or strong brown color. This study aimed to develop an effective and safe decoloring system for soy-based protein products using food-grade hydrogen peroxide and catalase. First, soy-based protein isolate (PI) and textured vegetable protein (TVP) were treated with hydrogen peroxide, and then the residual hydrogen peroxide was degraded using catalase. This process caused notable decolorization of PI and TVP, and residual hydrogen peroxide was not detected in these products. These findings indicate that this process could safely and effectively decolorize soy-based proteins. Interestingly, this decoloring process enhanced the solubility, water- and oil-holding capacities, foaming capacity, and emulsifying stability of decolored soy-based PI. Additionally, cooking loss and juiciness of decolored TVP-based foods were improved compared to those of non-treated foods. These findings indicate that the decoloring process also enhances the physical properties of soy-based protein products.

The Potential of Trigona spp. Propolis as an Antioxidant Agent to Reduce Residual Peroxide after Intra-Coronal Bleaching Treatments

The present study aimed to determine the effectiveness of Trigona spp. propolis as an antioxidant to reduce residual peroxide after intra-coronal bleaching treatments. Thirty-five maxillary central incisors were divided into seven groups: five samples without antioxidants; sodium ascorbate 10% combined with Tween 80 0.2%; and Trigona spp. propolis 10%. The lengths of the application time were 1 h, 24 h, and 48 h. Each application time consisted of five samples. Root resection followed by artificial discoloration was performed in the samples. Then, intra-coronal bleaching using 35% hydrogen peroxide was applied. After the tooth color changed, the bleaching material was cleared, and this was followed by the applications of sodium ascorbate 10% combined with Tween 80 0.2% and Trigona spp. propolis 10%. The peroxide residue was measured by assessing dissolved oxygen using a titration analysis with either the Winkler or iodometric method. Data were analyzed using the ANOVA test and Tukey’s HSD test. The lowest peroxide residue amount was found with the application of antioxidants for 48 h after the intra-coronal bleaching treatment using 35% hydrogen peroxide. However, there was no significant difference between sodium ascorbate 10% combined with Tween 80 0.2% and Trigona spp. propolis 10% to reduce peroxide residues after the intra-coronal bleaching treatment (p > 0.05). Therefore, these findings indicate that Trigona spp. propolis 10% effectively reduces peroxide residues after intra-coronal bleaching treatments, which can interfere with the bond of the composite to the tooth surface and shorten the wait time for composite restorations after bleaching treatments.

Optimal Time for Recirculation with Ultrafiltration to Remove Disinfectant in Reused Dialyzer.

The practice of dialyzer reuse is common in developing countries. One essential technique for safe dialyzer reprocessing is to remove disinfectants properly to the recommended standards before treatment initiation, using dialysis machine recirculation with ultrafiltration. This study was conducted to identify the most effective time for carrying out this procedure, and the factors affecting it. We studied 420 high-flux dialyzers with three different membrane types: FB210U (cellulose triacetate), F80S (polysulfone), and Elisio-210HR (polyethersulfone) at four reused cycles (5th, 10th, 15th, and 19th). Peracetic acid was used as the disinfectant at a concentration of 0.16%. The total cell volume (TCV) and clot inspection grade of the reused dialyzer were recorded before the procedure. The optimal time for disinfectant removal was independently determined by two observers using the residual peroxide strip test of the ultrafiltrate. We observed that disinfectant removal was dependent on the recirculation time and became undetectable at 5 minutes. The type of dialyzer had a significant effect on the effective recirculation time (shortest for FB210U followed by Elisio-210HR and F80S; p < 0.001), but the reused number, TCV, and clot inspection grade did not. It is conceivable that the dialyzer membrane type might affect peracetic acid removal. In conclusion, the interval for carrying out dialysis machine recirculation with ultrafiltration depends on the dialyzer type, and 5 minutes is the optimal time for the operation. This result can be implemented to improve further practice in dialyzer reprocessing.

Study of bleaching of old newsprint recycled paper: reproduction of newspaper material

With the utilization of used newspaper into the paper making industry, bleaching has been an area of concern to obtain a balance between desired brightness and the amount of treatment it requires. Motivation of this study was to optimize bleaching of recycled newsprint fibers in terms of five variables namely hydrogen peroxide charge, amount of sodium hydroxide employed, pulp consistency, bleaching temperature, and bleaching time. Experimental work was conducted in the laboratory to simulate bleaching plant and it was found that higher peroxide charge is better for improvement in brightness, but it is uneconomical to increase peroxide charge indefinitely. The amount of hydrogen peroxide is added so that the residual peroxide at the end of the reaction is not less than 10% of the initially added peroxide. Sodium hydroxide provides alkalinity which is very important for making the fibers flexible and softening of ink particles but it also contributes to pulp yellowing if residual hydrogen peroxide contents are lesser than the minimum required value. So, for 16 kg t−1 of hydrogen peroxide, 6 kg t−1 of sodium hydroxide prevented the brightness reversion in this study. Higher pulp consistency is better but unavailability of very high consistency pulp mixer in the industry is a limiting factor for higher than 30% pulp consistency. Temperature and time are quite interrelated and, with the given bleaching conditions, a temperature of 95 °C with 10 min residence time was found to be optimum for this study. It was also observed that multiple pre-washings increase brightness and save bleaching chemicals.

Accurate and Precise Protocol to Estimate the Activity of Peroxiredoxin Enzyme.

Accurate estimation of Prx activity poses many complications and interferences. The present protocol is free of interference and provides an effective alternative for the assessment of peroxide with high sensitivity. The assay can be used in clinical pathology laboratories since it is simple, rapid, and inexpensive. The systematic reagent consisted of AFS/ASA which acted as a sensitive probe for peroxide. Prx activity was estimated by incubating samples in suitable concentrations of 1,4-dithio-DL-threitol (DTT) and hydrogen peroxide (H2O2) or t-Butyl hydroperoxide (t-BOOH), as the substrates. The enzymatic reaction was inhibited after incubation with a working reagent containing ammonium ferrous sulfate (AFS) and aminosalicylic acid (ASA). Residual peroxide reacted with the working solution to form a brown-colored ferriaminosalicylate (FAS) complex with a maximum absorbance (λmax) of 425 nm. This protocol used sodium azide (NaN3) to eliminate catalase interference and avoided using high concentrations of strong acid to inhibit the Prx reaction. We concluded that the new protocol produced the same efficacy as the reference method since a strong correlation coefficient of comparison (r> 0.99) was found between both the FAS and ferrithiocyanate method.

Implications of COD analysis use in the peracetic acid-based wastewater treatment.

Peracetic acid (PAA) stands out as a safe and environmental-friendly oxidant and disinfectant which has been effectively used in wastewater treatment. Chemical oxygen demand (COD) is a very popular analysis in wastewater treatment; however, the interference of residual PAA on the COD measurement is still unknown. In this context, this study investigated the implications of applying the COD analysis in PAA-based treatment. Each 1 mg·L-1 of PAA increased the COD concentration around 13.5 mg O2·L-1. Residual PAA and hydrogen peroxide (H2O2) were efficiently neutralized by sodium metabisulfite (SMBS) at the optimal SMBS/PAA ratio of 10.2:1 in a wide pH range (5 to 9). The effect of PAA addition on the COD concentration was evaluated in different water matrices (potassium hydrogen phthalate and wastewater solutions). The COD results with the SMBS addition at optimal SMBS/PAA ratio were lower than the ones without it. It may happen due to the neutralization of residual H2O2/PAA and the complexity of the water matrices which can interfere in the COD results. This study discussed the impact of the residual H2O2/PAA neutralization before the COD analysis, and this investigation can be used as a practical guideline for the correct COD measurement in PAA-based treatment.

When treatment increases the contaminant's ecotoxicity: A study of the Fenton process in the degradation of methylene blue

The degradation of dyes can generate harmful by-products, thereby requiring the need to evaluate the toxicity to aquatic organisms. This study aims to evaluate the chronic ecotoxicity of methylene blue dye degraded by the Fenton process using the non-target planarian Girardia tigrina as a sensitive bioindicator of environmental contamination. The bioassays evaluated the lethality of several concentrations of the untreated and degraded dye methylene blue (MB), as well as, their sub-lethal effects on locomotion, feeding, regeneration, and reproduction. In both acute and chronic tests, the degraded dye had a stronger toxic effect when compared to the untreated dye. This negative effect after treatment was mainly associated with the presence of residual hydrogen peroxide and iron (and consequently the hydroxyl radical formed). We conclude that the utilization of the Fenton process using less oxidizing agents should be considered as important alternatives for the protection of aquatic ecosystems, without compromising the efficient removal of MB.

Evaluation of hydrogen peroxide and ozone residue levels on N95 masks following chemical decontamination

BackgroundHydrogen peroxide and ozone have been used as chemical decontamination agents for N95 masks during supply shortages. If left behind on the masks, the residues of both chemicals represent a potential health hazard by skin contact and respiratory exposure.AimCharacterization of hydrogen peroxide and ozone residues on mask surfaces after chemical decontamination.MethodsVarious N95 masks were decontaminated using two commercial systems employing either aerosol spray or vaporization of hydrogen peroxide in the presence of ozone. Following the decontamination, the masks were aired out to eliminate moisture and potential chemical residues. The residual hydrogen peroxide and ozone were monitored in the gas phase above the mask surface, and hydrogen peroxide residue directly on mask surfaces using a colorimetric assay.FindingsAfter decontamination, hydrogen peroxide and ozone were detectable in the gas phase in the vicinity of masks even after 5 h of aeration. Hydrogen peroxide was also detected on all studied masks, and levels up to 56 mg per mask were observed after 0.5 h of aeration. All residues gradually decreased with aeration, likely due to decomposition and vaporization.ConclusionHydrogen peroxide and ozone were present on N95 masks after decontamination. With appropriate aeration, the gaseous residue levels in the vicinity of the masks decreased to permissible levels as defined by the US Occupational Safety and Health Administration. Reliable assays to monitor these residues are necessary to ensure the safety of the mask users.

Photo-Fenton process under sunlight irradiation for textile wastewater degradation: monitoring of residual hydrogen peroxide by spectrophotometric method and modeling artificial neural network models to predict treatment

In this work, a procedure was elaborated to quantify hydrogen peroxide (H2O2) after degradation tests of dyes present in a synthetic textile matrix. For this purpose, based on the intensity of radiation absorption of the peroxovanadium cation, which was formed by the reaction between the oxidant and vanadate ions, ultraviolet/visible spectrophotometry technique was used. The most suitable experimental condition was composed of concentrations of 0.05 mol L−1 (NH4VO3) and 0.3 mol L−1 (H2SO4). The system for dye treatment involved the photo-Fenton process under simulated sunlight. In this case, concentrations of 900 mg L−1 (H2O2) and 4 mg L−1 (iron) in pH 3 were the most efficient for degrading contaminants. An efficiency of 94.49% was obtained after 180 min of reaction, the time in which the presence of the oxidant was no longer verified. The kinetic monitoring showed a two-stage degradation, described with accuracy greater than 96% by the linear and non-linear kinetic models of pseudo-first order. Additionally, the degradation under natural solar radiation was also studied, which resulted in an efficiency of 92.45% after 360 min and in the presence of the residual oxidant. Finally, via mathematical modeling and employing a Multilayer Perceptron neural network, with a 3-10-2 topology and BFGS 387 training algorithm, it was possible to predict the degradation and H2O2 residual concentration with an accuracy greater than 98%. Therefore, the degradation study developed and the proposed methodology for determining residual H2O2 proved to be adequate and capable of contributing positively to related research.

Advanced treatment of dye wastewater using a novel integrative Fenton-like/MnO2-filled upward flow biological filter bed system equipped with modified ceramsite

The deposal of residual hydrogen peroxide (H2O2) in Fenton-like system and the requirement of oxygen in bioreactor are essential parts for the treatment of integrative Fenton-like/bioreactor. A novel low-cost integrative Fenton-like and MnO2-filled upward flow biological filter bed (Fenton-like/MBFB) equipped with the modified ceramsite was constructed to evaluate the main properties and catalytic activity of modified ceramsite, and the optimal conditions of integrative system and compare integrative and traditional systems. In this study, the Fenton-like reactor with modified ceramsite had higher catalytic ability whose Acid Orange 7 (AO7) degradation efficiency reached to 79.3% due to large surface area and high porosity, compared with that with raw ceramsite (44.3%). Furthermore, total utilization efficiency of H2O2 in integrative system (from 32.41% to 53.51%) and removal efficiencies of COD and AO7 were remarkably improved, which would effectively decrease the waste of H2O2 and the setting of regulation pool and aeration tank. Thus, the integrative system can save 0.51 CNY/m3 in construction cost and 0.21 CNY/m3 in operating cost. The average COD removal efficiency, AO7 degradation efficiency and effluent DO concentration were achieved to 64.8%, 79.5% and 9.3 mg/L respectively in integrative system were achieved in integrative system during sixty successive runs. Also, the potential degradation pathway of contaminants was also proposed according to the OH-enhanced at Fenton-like reactor due to catalyst and adsorption of modified ceramsite and the removal of microorganisms and modified ceramsite for contaminants at MBFB. This study demonstrated the feasibility of integrative Fenton-like/MBFB filled with modified ceramsite for simultaneously decreasing operational cost and complexity and enhancing removal efficiency, thus provided a one-step alternative for refractory dye wastewater.