Resacetophenone Oxime Research Articles

Synthesis, characterization, electrochemical studies, DNA binding, and cleavage activity of mixed copper(II)oxime DMSO complexes

Mixed ligand complexes having molecular formulae Cu(L)2(DMSO)2 [where L ¼ salicylaldoxime (SAO), 2-hydroxyacetophenone oxime (HAO), and resacetophenone oxime (RPO)] have been synthesized and characterized by molar conductivity, magnetic susceptibility, UV–vis, IR, and ESR methods. The electronic and ESR spectra suggest distorted octahedral geometry for mixed ligand complexes. From ESR data all the complexes follow the order gjj 4g? 4 2.0037 showing covalent bonding nature and copper(II) (d 9 ) ion in axial symmetry with unpaired electron in dx27y2 orbital. Cyclic voltammetric studies show quasireversible reduction attributable to Cu 2þ /Cu þ . Absorption titration studies revealed that each complex is an avid binder of CT DNA. The apparent binding constants are in the range 3.2 6 10 5 to 2.4 6 10 6 . The cleavage activity of complexes was carried out on double-stranded pBR322 circular plasmid DNA using gel electrophoresis. All complexes show increased nuclease activity in the presence of oxidant (H2O2) and cleavage is strongly dependent on time of incubation and concentration of complex. Control cleavage experiment carried out in the presence of potential inhibitors, viz. dimethyl sulfoxide, tert-Butyl alcohol, ethylenediaminetetraacetic acid and NaN3, inhibits cleavage activity suggesting an oxidative mechanism with major active species involving . OH in cleavage of plasmid DNA.

Synthesis, characterization, DNA interaction and cleavage activity of new mixed ligand copper(II) complexes with heterocyclic bases

Mixed ligand complexes having the formulae Cu(RPO) 2Py 2, Cu(RPO) 2Im 2 and Cu(DBO) 2Py 2 [RPO = resacetophenone oxime, DBO = 2,4-dihydroxybenzophenone oxime, Py = pyridine and Im = imidazole] have been synthesized and characterized by UV–Vis, IR, ESR, cyclic voltammetry and magnetic susceptibility methods. Absorption studies revealed that each of these octahedral complexes is an avid binder of calf thymus DNA. The apparent binding constants for mixed ligand complexes are in order of 10 4–10 5 M −1. Based on the data obtained in the DNA binding studies a partial intercalative mode of binding is suggested for these complexes. The nucleolytic cleavage activity of the adducts was carried out on double stranded pBR322 circular plasmid DNA by using a gel electrophoresis experiment in the presence and absence of oxidant (H 2O 2). All the metal complexes cleaved supercoiled DNA by hydrolytic and oxidative paths. The oxidative path dominates the hydrolytic cleavage. The hydrolytic cleavage of DNA is evidenced from the control experiments showing discernable cleavage inhibition in the presence of the hydroxyl radical inhibitor DMSO or the singlet oxygen quencher azide ion.

Simultaneous determination of ruthenium, osmium and palladium by reversed-phase HPLC using 4-(2′-thiazolylazo) resacetophenone oxime as chelating reagent

The chelates of Ru, Os and Pd with 4-(2′-thiazolylazo) resacetophenone oxime were separated simultaneously by HPLC using a pre-column derivatization method, at the wavelength 560 nm, on an octadecly-bonded silica stationary phase with a mobile phase methanol—water mixture (40–60 v/v) containing 40 m M of acetate buffer pH 5.0. The detection limits for Ru, Os and Pd were 2.0, 4.0 and 0.2 ng, respectively. The method has been applied successfully for the determination of metal ions in an anode slime.

Simultaneous determination of uranium and thorium using 4-(2′-thiazolylazo)resacetophenone oxime as analytical reagent. A derivative spectrophotometric method

A first derivative spectrophotometric method has been developed for the simultaneous determination of uranium(VI) and thorium(IV) when present in the same solution using 4-(2′-tiazolylazo)resacetophenone oxime (TARPO) as analytical reagent without prior separation of any of the metals or using masking agents, etc. The method allows the determination of uranium and thorium in their mixtures in the range of 0–10 ppm each. Many common anions and cations even in large quantities do not interfere.

Catalytic method for the determination of trace amounts of mercury in environmental samples

A catalytic method based on the abstraction of CN– from hexacyanoferrate(II) by mercury(II) in the presence of 4-(2′-thiazolylazo) resacetophenone oxime was studied spectrophotometrically. The method allows the determination of mercury(II) in the range 0.01–0.9 ppm at a wavelength of 420 nm. The method was applied to the determination of methyl- and ethylmercury chloride and the analysis of environmental water samples.

Homogeneous isotope exchange between copper(II) and bis(resacetophenone oxime)copper(II) complex

Isotope exchange behavior of bis(resacetophenone oxime)copper(II) complex with copper(II) in tri-n-butyl-phosphate and methanol medium has been studied. The studies were carried out at different temperatures varying the concentration of both metal ion and complex. The results show that the complex is labile in the kinetic sense. Increase in temperature increases the isotope exchange rate. The increase in concentration also results in enhancement of the rate of reaction.

Rapid and selective spectrophotometric determination of manganese in steels and alloys using resacetophenone oxime

A sensitive spectrophotometric method is developed for the determination of manganese in aqueous medium. The metal ion forms a yellowish brown coloured complex with resacetophenone oxime (RPO) in ammonium chloride and ammonium hydroxide buffer of pH 10.5. The 1:1 complex shows maximum absorbance at 380 nm with a Beer's law range of 0.09–1.7 ppm. The molar absorptivity and the Sandell sensitivity are found as 2.5 × 10 4 l.mole −1.cm −1 and 0.002 μg/cm 2, respectively. The stability constant of the complex calculated by Job's method is 7.5 × 10 5. The interfering effects of various cations and anions are studied. The present method is applied to the determination of manganese in some steel and alloy samples.

Simultaneous determination of uranium and thorium by second-derivative spectrophotometry

A second-derivative spectrophotometric method has been developed for the simultaneous determination of uranium and thorium using 4-(2′-thiazolylazo) resacetophenone oxime as the spectrophotometric reagent. The method allows the determination of uranium and thorium in the range 0–10.0 ppm. The relative error is less than 4%. Common anions and cations do not interfere in the determination even when present in large amounts. However, for transition metal ions, viz., Cu, Ni, Pd and Pt, the tolerance limit is less than 100 ppm.

Homogeneous isotopic exchange between nickel(II) and bis(resacetophenone oxime) nickel(II) complex

Isotopic exchange behaviour of bis(resacetophenone oxime) nickel(II) complex with nickel(II) in tri-n-butyl phosphate (TBP) and ethanol medium has been studied. The studies were carried out at different temperatures by varying the concentrations of both metal ion and the complex. Experimental observations showed that the complex is kinetically labile. Increase in temperature increases the isotopic exchange rate. Increase in concentration of either metal ion or complex results in significant increase of the reaction rate.

4-(21-thiazolylazo) resacetophenone oxime. A new analytical reagent for the spectrophotometric determination of uranium (VI)

4-(21-Thiazolylazo) resacetophenone oxime forms a pink colored soluble complex with uranium(VI) in buffer solutions of pH 6.0. The colored complex has a maximum absorbance at the wavelength 572 nm and the color is stable for about 48 h. The system obeys Beer's law over the concentration range 0.2–6.0 μg of uranium cm−3. The molar absorptivity and the Sandell sensitivity of the complex are 6.2×104 dm3.mol−1.cm−1 and 0.0038 μg cm−2, respectively. Effect of various diversions has been studied and the method was successfully applied for the determination of uranium in rock samples.

Formation constant of cobalt/II/ with resacetophenone oxime

Formation constant of cobalt/II/ with resacetophenone oxime

Synergistic extraction of cobalt(II) with resacetophenone oxime in the presence of heterocyclic nitrogen and organophosphorous compunds

Equilibrium distribution coefficients have been determined, for the extraction of cobalt(II) with resacetophenone oxime as a function of pH and reagent concentration at ambient temperature. RAPOX forms simple 1∶2 chelate with cobalt(II). Synergistic extraction was observed with pyridine and its methyl derivatives and some organophosphorous compounds. From the extraction equilibrium data, the adduct formation constants of 1∶2 chelate to adducting ligand adducts were evaluated.

Reactions of cobalt resacetophenone oximate with heterocyclic nitrogen bases

Adduct formation constants of the pyridine adducts of the cobalt(II) chelate of resacetophenone oxime in cyclohexanone have been determined spectrophotometrically. Spectral changes in the absorbance were observed in visible range. The stability of the cobalt adducts increases in the following order of the bases: pyridine<4-picoline<3,5-lutidine<1,10-phenanthroline<2,9-neocuproin<2,2′-bipyridyl.

ChemInform Abstract: Metal Chelates of Resacetophenone Oxime

Chemischer InformationsdienstVolume 17, Issue 34 Reviews ChemInform Abstract: Metal Chelates of Resacetophenone Oxime K. S. BHATKI, K. S. BHATKISearch for more papers by this author K. S. BHATKI, K. S. BHATKISearch for more papers by this author First published: August 26, 1986 https://doi.org/10.1002/chin.198634372Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat No abstract is available for this article. Volume17, Issue34August 26, 1986 RelatedInformation

Resacetophenone oxime–formaldehyde resin and its metal chelates. III

AbstractIn this paper the synthesis and properties of resacetophenone oxime–formaldehyde resin are described. The number‐average molecular weights of the resin determined by vapor‐pressure osmometry and nonaqueous conductometric titration are 2200 ± 10% and 2500 g/mol, respectively. Cu(II), Ni(II), and Co(II) chelates of the resin were prepared. Elemental analysis of the chelates indicates a metal to ligand ratio of 1:2. The diffuse reflectance spectra and magnetic moments of Ni(II) and Co(II) chelates show tetrahedral structure, whereas Cu(II) chelate shows a square planar structure. Infrared spectral studies show that the metals are coordinated through the nitrogen of the oximino group and oxygen of the phenolic group. The presence of methylene bridges is also indicated by IR spectral studies. The thermal behavior of the resin and its metal chelates are also discussed.

Potentiometric titration of permanganate with resacetophenone oxime

Milligram amounts of permanganate can be titrated with resacetophenone oxime (4-acetylresorcinol oxime) as a reducing titrant in the presence of phosphoric acid (0.5 M). The stoichiometry between permanganate and the oxime is 3:1 (MnO 4 -:oxime). The titration is successful in the presence of large amounts of dichromate or vanadate or moderate amounts of cerium(IV).

Amperometric determination of palladium

Palladium (II) can be determined within + 0.5% in presence of nickel by amperometric titration with resacetophenone oxime either by (i) adding an excess of the oxime and titrating the excess with copper (II) in sodium acetate-acetic acid buffer; or by (ii) a direct titration using chloroform to prevent the palladium complex coming in contact with the mercury pool. However, this method cannot be adopted for the simultaneous determination of Pd and Ni.

Gallacetophenone oxime as a reagent for gravimetric determination of copper and its separation from other elements

Gallacetophenone oxime is a more useful analytical reagent for the determination of copper in presence of nickel, cadmium, zinc, manganese, selenium and iron than either salicylaldioxime or resacetophenone oxime. It is more soluble in water and its gravimetric factor (0.1486) is more favourable than with either salicylaldioxime (0.1895) or resacetophenone oxime (0.1607).

Preparation of carrier-free copper-64,67 nuclides by liquid-liquid extraction

A simple procedure is described for the preparation of carrier-free copper-64,67 nuclides from a zinc target exposed to fast neutrons. The irradiated target is dissolved in water, and thepH of the solution is adjusted to about six. Radioactive copper nuclides are then extracted with a 1% solution of resacetophenone oxime in cyclohexanone. Zinc is not extracted under these conditions. Radiocopper is back-extracted by shaking the organic phase with dilute mineral acid. About 5 mCi of carrier-free64Cu can be obtained from 1 g of zinc.

Stability constants of complexes of resacetophenone oxime.

Stability constants of complexes of resacetophenone oxime.