This study explores the pollen diversity and viability of angiosperms within Akot, Akola District, encompassing a total of 44 plant species from 28 families. Through comprehensive analyses of pollen morphology and viability, we aimed to shed light on the reproductive strategies of these plants. Utilizing standardized methodologies, we conducted in-depth examinations of pollen grains from various angiosperm species. Our findings reveal a wide array of morphological variations among pollen grains, with diameters ranging from 13.63 µm to 122.67 µm. additionally; we assessed pollen viability, with percentages ranging from 20% to 92.85%, providing insights into the reproductive health and success of these plant species. This research contributes to a deeper understanding of angiosperm reproductive biology and underscores the importance of conservation efforts to preserve floral ecosystems.