Renewable Carbon Source Research Articles

Cascade Catalytic Systems for Converting CO2 into C2+ Products.

The excessive emission and continuous accumulation of CO2 have precipitated serious social and environmental issues. However, CO2 can also serve as an abundant, inexpensive, and non-toxic renewable C1 carbon source for synthetic reactions. To achieve carbon neutrality and recycling, it is crucial to convert CO2 into value-added products through chemical pathways. Multi-carbon (C2+) products, compared to C1 products, offer a broader range of applications and higher economic returns. Despite this, converting CO2 into C2+ products is difficult due to its stability and the high energy required for C-C coupling. Cascade catalytic reactions offer a solution by coordinating active components, promoting intermediate transfers, and facilitating further transformations. This method lowers energy consumption. Recent advancements in cascade catalytic systems have allowed for significant progress in synthesizing C2+ products from CO2. This review highlights the features and advantages of cascade catalysis strategies, explores the synergistic effects among active sites, and examines the mechanisms within these systems. It also outlines future prospects for CO2 cascade catalytic synthesis, offering a framework for efficient CO2 utilization and the development of next-generation catalytic systems.

Utilizing Pistachio Shell Biochar to Replace Carbon Black in Natural Rubber Composites

Biochar is a promising source of renewable carbon that potentially can serve the same purpose as carbon black (sourced from fossil fuels) to reinforce rubber composites. Pistachio shells are a prolific agricultural waste product that is a suitable feedstock for biochar. Unlike many other agricultural residues, pistachio shells are a feedstock that yields biochar with a high concentration of carbon (>80%) and low concentration of ash (<5%), which is necessary to replace carbon black without detrimental effects to the final composite. Filler blends of pistachio shell biochar and carbon black were explored to see how much carbon black could be replaced before composite properties were affected. Pistachio shell biochar was able to replace up to 40% of the carbon black while improving the tensile strength, elongation, and toughness of the rubber composites, but a reduction in modulus was observed. Based on the results obtained, pistachio shell biochar would be suitable for partially replacing carbon black in applications like hoses, seals, belts, and gloves, thereby enabling a new application for this sustainable, agricultural waste product that will help reduce dependence on fossil fuels.

Industrial–scale production of various bio–commodities by engineered microbial cell factories: Strategies of engineering in microbial robustness

The utilization of renewable, non–edible biomass for synthesis of valuable bio–products including bio–fuels, bio-chemicals and bio–polymeric materials, in an environmentally sustainable manner is crucial for addressing the urgent environmental challenges caused by our substantial dependence on fossil fuel resources. In this context, engineered microbial cell factories (MCFs), which are the modified microorganisms, have gained attention and provide biosynthetically optimized pathways for the production of desired bio–commodities using renewable carbon sources. Biosynthetic routes for the production of such bio–commodities can be categorized into three groups based on the chosen microbial host for genetic modification: native, non–native, and artificial pathways. Engineered MCFs are increasingly essential in the pharmaceutical, food, and bio–chemical industries and are being developed to address the growing world population and socioeconomic crisis. Mainly, microorganisms have been utilized in the manufacturing of a range of bio–products including amino acids, carboxylic acids, carotenoids, enzymes, vitamins, plant natural products, biogas, and other biofuels. Furthermore, the implementation of advanced metabolic engineering and synthetic biology tools & techniques enhances the speed, concentration, and efficiency of commercially important substances by modifying the metabolism, carbon–energy balance and eliminating an undesired ATP sink, physiology, and stress response. All these has resulted in rapid growth of industrial biotechnology for production of several bio–commodities. Further, the scale and numbers of bio–commodities is increasing with time. This review summarizes the design of MFCs, selection of microbial strains, metabolic pathways, engineered MCFs for industrial–scale applications, strategies for engineering microbial robustness, commercial restrictions, and their future prospects.

Sustainable supercapacitors of sulfur-doped carbon from environmentally friendly sodium lignosulfonate impregnated with bacterial cellulose

The use of sustainable natural sources to fabricate porous carbon materials has garnered significant interest in energy storage. This study proposes a method for synthesizing sulfur-doped porous carbon (S‑carbon) materials via the carbonization of bacterial cellulose (BC) impregnated with sodium lignosulfonate (LS), which functions as a renewable source of both carbon and sulfur, eliminating the need for external activation processes. The carbonization process yielded S‑carbon with a notable sulfur content of 1.4 % and a high specific surface area of 650 m2/g. Transmission electron microscopy (TEM) images reveal fibrous carbon structures with mesopores and micropores in the S‑carbon material. Electrochemically, S‑carbon exhibited an impressive specific capacitance of 272.6 F g−1 at a current density of 1 A/g and demonstrated outstanding cycling stability, retaining 86 % of its capacitance after 5000 cycles at a current density of 6 A/g in a 3 M KOH electrolyte. The development of sulfur-doped fibrous carbon from BC-LS offers promising potential as a sustainable electrode material for supercapacitors.

Concatenating Microbial, Enzymatic, and Organometallic Catalysis for Integrated Conversion of Renewable Carbon Sources

Concatenating Microbial, Enzymatic, and Organometallic Catalysis for Integrated Conversion of Renewable Carbon Sources

2D-3D electron transfer functions and stability of sustainable graphitic biocarbon for bipolar plate application

Biocarbon being a highly demanding renewable source of carbon is important for many applications such as soil enrichment, electronic applications, etc. In this research, sustainable waste biomass-to-energy materials conversion, kinetic, thermodynamic and electronic properties of carbonized forest biomaterials were investigated to evaluate their high-potential in bipolar plate for fuel cell application. In thermogravimetric analysis, the lignin biocarbon showed the least activation energy of 95 KJ/mol compared to 127 and 145 KJ/mol for hardwood and softwood biocarbons respectively. The crystallographic nature of carbonized ligneous and cellulosic biomaterials was also investigated, showing its intrinsic properties and exotic functionality through semi-metallic properties determined from density function theory, transmission electron microscopy and UV–Vis absorption. Finally, the electrochemical properties of bio-carbon composites were examined to prove stability and corrosion resistance comparable to metallic plates. Biocarbon composites showed high polarization resistance up to 5.96 kΩ-cm2 with non-reactive properties, favorable to use in bipolar plates as an alternative to metallic plate which is expensive and prone to corrosion. Overall, sustainable biocarbon shows its ability as a high-performance functional material alternative to expensive nanofillers as well as to enhance the attributes of the bipolar plate composite by increasing connectivity between primary filler and insulating resin.

CO2 Conversion by Oxygen-Enriched Gasification of Wood Chips

With increasing efforts to lower CO2 emissions globally, the demand for carbon-based resources in industries remains on a high level, leading to new technologies being able to provide those essential carbon sources. To the best of our knowledge, we were able to show for the first time the adaption of a readily available gasifier for the gasification of wood chips using only O2 (18.4–23.1 Vol.-%) and CO2 as gasification agents, creating a nitrogen-free product gas. It was found that the setup used was able to convert up to 27.2% of the CO2 from the gasification agent to CO, creating a promising route for the production of renewable carbon sources for future carbon-based applications. Furthermore, no decrease in gasification performance was observed as the cold gas efficiency was at 83.5–95.5% with only minor formation of tar.

Alkoxyhydrosilane-facilitated cross-etherification reaction of secondary benzyl alcohol with aliphatic alcohol: synthesis of unsymmetrical dialkyl ethers.

Alkoxyhydorosilane is found to be an effective mediator for the cross-etherification reaction between two distinct alcohols, namely, a secondary benzyl alcohol and an aliphatic alcohol, providing the unsymmetrical dialkyl ethers in good-to-high yields. The reaction is also successfully applied to the lignin model compound, which is an important renewable non-fossil organic carbon source. Initial mechanistic studies indicated that the carbocation derived from benzyl alcohol was formed under the present reaction conditions.

Enterobacter spp. isolates from an underground coal mine reveal ligninolytic activity

Lignin, the second most abundant renewable carbon source on earth, holds significant potential for producing biobased specialty chemicals. However, its complex, highly branched structure, consisting of phenylpropanoic units and strong carbon-carbon and ether bonds, makes it highly resistant to depolymerisation. This recalcitrancy highlights the need to search for robust lignin-degrading microorganisms with potential for use as industrial strains. Bioprospecting for microorganisms from lignin-rich niches is an attractive approach among others. Here, we explored the ligninolytic potential of bacteria isolated from a lignin-rich underground coalmine, the Morupule Coal Mine, in Botswana. Using a culture-dependent approach, we screened for the presence of bacteria that could grow on 2.5% kraft lignin-supplemented media and identified them using 16 S rRNA sequencing. The potential ligninolytic isolates were evaluated for their ability to tolerate industry-associated stressors. We report the isolation of twelve isolates with ligninolytic abilities. Of these, 25% (3) isolates exhibited varying robust ligninolytic ability and tolerance to various industrial stressors. The molecular identification revealed that the isolates belonged to the Enterobacter genus. Two of three isolates had a 16 S rRNA sequence lower than the identity threshold indicating potentially novel species pending further taxonomic review. ATR-FTIR analysis revealed the ligninolytic properties of the isolates by demonstrating structural alterations in lignin, indicating potential KL degradation, while Py-GC/MS identified the resulting biochemicals. These isolates produced chemicals of diverse functional groups and monomers as revealed by both methods. The use of coalmine-associated ligninolytic bacteria in biorefineries has potential.

A case study of the regional bioeconomy in Central Germany: construction with renewable resources

In response to global challenges like climate change and resource depletion, national and international policies are increasingly emphasising sustainability. The bioeconomy represents a promising field to reconcile environmental integrity with economic development. The objective of this study is to examine the role of renewable resources in the construction industry within the framework of the bioeconomy, with a particular focus on Sustainable Development Goal 11: ‘Sustainable cities and communities’. The case study analyses the practical challenges and opportunities for innovation in Central Germany through a comprehensive regional analysis of construction practices using renewable resources. The methodology involved monitoring biomass flows by assessing harvest yields, area shares and biomass potentials. It also analysed the importance of the bio-based economy by examining employment data and identifying potential growth sectors. Stakeholder engagement was facilitated through knowledge transfer workshops to encourage collaboration and innovation. The results of the study highlight key trends within the industry, including the increasing prevalence of timber buildings, wood as a renewable carbon source in the chemical industry and the growing demand for wood-based packaging solutions. Policy initiatives are playing a supportive role in driving these developments, taking advantage of the region’s extensive wood resources (14.7 million m3 harvested in 2021) and the significant employment contribution of the forestry, wood and construction industries (2% of total employment, representing no less than 81,506 employees). These figures demonstrate the potential for sustainable construction practices to drive local economic growth and contribute to wider environmental objectives.

Resolving the metabolism of monolignols and other lignin-related aromatic compounds in Xanthomonas citri

Lignin, a major plant cell wall component, has an important role in plant-defense mechanisms against pathogens and is a promising renewable carbon source to produce bio-based chemicals. However, our understanding of microbial metabolism is incomplete regarding certain lignin-related compounds like p-coumaryl and sinapyl alcohols. Here, we reveal peripheral pathways for the catabolism of the three main lignin precursors (p-coumaryl, coniferyl, and sinapyl alcohols) in the plant pathogen Xanthomonas citri. Our study demonstrates all the necessary enzymatic steps for funneling these monolignols into the tricarboxylic acid cycle, concurrently uncovering aryl aldehyde reductases that likely protect the pathogen from aldehydes toxicity. It also shows that lignin-related aromatic compounds activate transcriptional responses related to chemotaxis and flagellar-dependent motility, which might play an important role during plant infection. Together our findings provide foundational knowledge to support biotechnological advances for both plant diseases treatments and conversion of lignin-derived compounds into bio-based chemicals.

Ru-doped Mo2C grown on coconut shell derived 3D hierarchical carbon as robust electrocatalysts for hydrogen evolution reaction

Biomass, as a low-cost, environmental friendly and renewable carbon source with natural porous structure can be used as carriers. Molybdenum carbide (Mo2C), possessing metallic characteristics and a platinum-like d-band electronic structure, has recently emerged as promising candidate for hydrogen evolution reaction (HER). In this study, we fabricate a new electrocatalyst by embedding ruthenium (Ru) into Mo2C on carbonized coconut shell (Ru-Mo2C@CSC). Doping of Ru into Mo2C significantly activates the inherent HER activity by altering the surface state, which enhances electron accumulation and transfer between the intermediate and electrocatalyst surface. The obtained Ru-Mo2C@CSC exhibit a remarkably low overpotential of 40 mV at 10 mA cm−2 in 1 M KOH, matching the benchmark Pt/C. Both experimental and density functional theory (DFT) reveal that this outstanding electrocatalytic activity stems from its unique three-dimensional (3D) architecture and the altered electronic structure by Ru-doping. The distinct 3D hierarchical porous structure aids electrolyte penetration, increases exposure of active sites, and facilitates the release of gas products. Substituting Ru atoms notably diminish the proton adsorption strength at the Mo and C atom coordination sites and electronic modification of Mo by charge transfer from Ru to Mo which was observed by X-ray photoelectron spectroscopy. A synergetic interaction at the Ru-Mo interface is generated to optimize the adsorption–desorption energetics toward H intermediate and accelerate water dissociation.

Enzymatic promiscuity and underground reactions accounted for the capability of Escherichia coli to use the non-natural chemical synthon 2,4-dihydroxybutyric acid as a carbon source for growth

2,4-dihydroxybutyric acid (DHB) and 2-keto-4-hydroxybutyrate (OHB) are non-natural molecules obtained through synthetic pathways from renewable carbon source. As they are structurally similar to lactate and pyruvate respectively, they could possibly interfere with the metabolic network of Escherichia coli. In fact, we showed that DHB can be easily oxidized by the membrane associated L and D-lactate dehydrogenases encoded by lldD, dld and ykgF into OHB, and the latter being cleaved into pyruvate and formaldehyde by several pyruvate-dependent aldolases, with YagE being the most effective. While formaldehyde was readily detoxified into formate, Escherichia coli K12 MG1655 strain failed to grow on DHB despite of the production of pyruvate. To find out the reason for this failure, we constructed a mutant strain whose growth was rendered dependent on DHB and subjected this strain to adaptive evolution. Genome sequencing of the adapted strain revealed an essential role for ygbI encoding a transcriptional repressor of the threonate operon in this DHB-dependent growth. This critical function was attributed to the derepression of ygbN encoding a putative threonate transporter, which was found to exclusively transport the D form of DHB. A subsequent laboratory evolution was carried out with E. coli K12 MG1655 deleted for ΔygbI to adapt for growth on DHB as sole carbon source. Remarkably, only two additional mutations were disclosed in the adapted strain, which were demonstrated by reverse engineering to be necessary and sufficient for robust growth on DHB. One mutation was in nanR encoding the transcription repressor of sialic acid metabolic genes, causing 140-fold increase in expression of nanA encoding N-acetyl neuraminic acid lyase, a pyruvate-dependent aldolase, and the other was in the promoter of dld leading to 14-fold increase in D-lactate dehydrogenase activity on DHB. Taken together, this work illustrates the importance of promiscuous enzymes in underground metabolism and moreover, in the frame of synthetic pathways aiming at producing non-natural products, these underground reactions could potentially penalize yield and title of these bio-based products.

Orthophosphate uptake onto woodchips in denitrifying bioreactors is enhanced by anoxic-(anaerobic-)oxic cycling

Woodchips are widely used as a low-cost and renewable organic carbon source for denitrifying biofilms in passive nutrient removal systems. One limitation of wood-based biofiltration systems is their relatively poor removal of phosphorus (P) from subsurface drainage and stormwaters, necessitating the use of additional filter media when co-treatment of nitrogen (N) and P is required. Here, we show that anoxic-oxic cycling of woodchip media, which enhances nitrate (NO3−) removal by increasing the mobilization of organic carbon from wood, also improves orthophosphate (Pi) uptake onto woodchips. Orthophosphate removal rates in flow-through woodchip columns ranged from 0 to 34.9 μg PO43− L−1 h−1 under continuously-saturated (anoxic) conditions, and increased to 17.5 to 71.9 μg PO43− L−1 h−1 in columns undergoing drying-rewetting (oxic-anoxic) cycles. The highest Pi removal efficiencies were observed in the first 20 h after reactors were re-flooded, and were concurrent with maxima in polyphosphate kinase (ppk) gene expression by the polyphosphate accumulating organisms (PAOs) Accumulibacter spp. and Pseudomonas spp. Batch experiments confirmed that anoxic-anaerobic-oxic pre-incubation conditions led to orthophosphate uptake onto woodchips as high as 74.9 ± 0.8 mg PO43−/kg woodchip, and batch tests with autoclaved woodchips demonstrated that Pi uptake was due to biological processes and not adsorption. NO3− removal in batch tests was also greatest under oxic incubation conditions, attributed to greater carbon availability in hypoxic to anoxic zones in woodchip biofilms. While further research is needed to elucidate the mechanisms controlling enhanced Pi uptake by woodchip biofilms under anoxic-(anaerobic-)oxic cycling, these results suggest a role for enhanced Pi uptake by PAOs in a nature-based system for treatment of nonpoint source nutrients.

A cradle-to-grave assessment of carbon and energy footprints of ships using synthetic fuel for net-zeros operations

PurposeAchieving net-zero emissions in the maritime sector requires adopting carbon-neutral practices in terms of shipbuilding, operation and recycling. However, research integrating life cycle and energy analysis is missing to inform decision-makers. MethodConsequently, the paper presents models combining a model-based life cycle assessment (MBLCA) and ship energy simulations to examine the footprints of a generic New Panamax Containership (DTC). Analyses were conducted to assess the energy and carbon effects of using synthetic methanol as marine fuel. ResultsThe life-cycle analysis confirms the overwhelming prevalence of operations in the total greenhouse gases emission during the ship's lifetime. The analysis highlights the potential of carbon savings if the vessel consumes methanol produced from neutral energy and renewable carbon sources. However, the magnitude of shipping fuel requirements may profoundly impact energy infrastructures while still requiring large volumes of carbon offset credits. ConclusionThe paper confirms the usefulness of integrating life-cycle analysis and energy modelling to discuss future energy scenarios. The completeness of the models also allows for future research analysing other energy solutions.

Fischer-Tropsch Synthesis for the Production of Sustainable Aviation Fuel: Formation of Tertiary Amines from Ammonia Contaminants.

Fischer-Tropsch synthesis combined with product workup is a promising route toward synthetic aviation fuel from renewable hydrogen and carbon sources like biomass, CO2, and waste. Cost savings can be achieved by reducing the number of gas treatment steps in new plants, but the consequence of contaminants in the feed needs investigation. While feeding 2.6 ppmV ammonia to a Fischer-Tropsch reactor, it was shown that ammonia was predominantly chemically converted into organic amines, with most nitrogen found in the water phase (89%), followed by heavy wax (7%) and light wax (1%). The concentration difference between water and light wax was shown to be due to the post-condensation separation of amines on polarity. Amines up to a chain length of 120 were detected in the heavy wax with MALDI-FT-ICR-MS, which, in combination with the high nitrogen content, suggests that amines have a similar chain growth probability compared to the main hydrocarbon products. Detailed product analysis with three independent analytical techniques showed that tertiary N,N-dimethylalkylamines were by far the most abundant amine class. This suggests that ammonia is decomposed on the cobalt surface and, potentially as a dimethylamine fragment, incorporated in the growing chain. Further evidence was obtained from the abundance of trimethylamine and from the reconciled nitrogen product analysis up to C100, which showed that the amine product distribution followed from naphtha onward the same ASF kinetics as alkanes and oxygenates while being distinctively different from the alkene distribution. The presented findings provide further avenues for studies of the Fischer-Tropsch reaction mechanism and indicate the opportunity of cost saving on gas treatment, while further validation is required to assess the impact on hydrocracking and product quality.

Synthesis and Characterization of Nanocellulose/Polyacrylonitrile‐based Carbon Nanofibers: An Approach toward Low Cost and Sustainable Agro Waste based Carbon Nanomaterials

AbstractThe cost of polymer precursors and high energy need in carbonization for the preparation of carbon materials are two limiting factors in the commercialization of carbon‐based materials. In this study, carbon nanofiber films were prepared by adding a very small concentration of polymer polyacrylonitrile in nanocellulose (extracted from agro waste i.e., rice straws) with an overall aim of producing carbon nanomaterials with cost effective, renewable and sustainable carbon source. Polyacrylonitrile (PAN) added nanocellulose solution was drop‐casted to prepare Nanocellulose/Polyacrylonitrile‐based nanofibers film (NC/PAN). It was further stabilized in air atmosphere at 280 °C followed by carbonized in nitrogen atmosphere at 700 °C. It was found that addition of NC/PAN have 18 % lower activation energies than PAN. Results of tunneling electron microscopy showed that polyacrylonitrile/nanocellulose films carbonized at 700 °C temperature exhibited amorphous carbon nanofibers structure similar to bare polyacrylonitrile based carbon film carbonized at higher temperature of 1000 °C‐1200 °C. Furthermore, polyacrylonitrile/nanocellulose based carbon nanofibers have showed high degree of carbonization ((ID)/(IG)=0.87) as compared to bare polyacrylonitrile based carbon nanofibers film ((ID)/(IG)=1.02). This may be attributed to chiral nature of nanocellulose which served as a template to orientation of graphene planes and showed efficient carbonization.

Sustainable production of yeast oil from rice straw hydrolysate by Pseudozyma parantarctica through fed-batch cultivation for bio-polyurethane foam formation

Rice straw serves as an alternative feedstock for yeast oil (YO) production and bio-polyurethane (BPU) foam formation. This study optimized yeast growth and YO production by Pseudozyma parantarctica, utilizing rice straw hydrolysate (RSH) as the primary carbon source. Fed-batch strategies were explored to enhance YO production efficiency and its conversion into BPU foam. The optimized conditions proved highly effective in yielding biomass and YO from RSH-OPM. The fed-batch approach significantly improved biomass, YO production, and oil content to 7.35 ± 0.18 g/L, 3.77 ± 0.09 g/L, and 51.21 ± 0.15 % (w/w), respectively. The main fatty acid (FA) compositions of YO consisted of C:16 to C:18 FAs for over 92 %. RSH-based YO was successfully transformed into both rigid and semi-rigid BPU foams. This research demonstrated the feasibility and potential of using RSH as a low-cost and renewable carbon source for YO production and BPU foam formation.

Efficient production of ginsenoside Rh2 from xylose by remodeling metabolism in Saccharomyces cerevisiae

Ginsenoside Rh2, a promising candidate for cancer prevention and therapy, has applications in the fields of health products and pharmaceuticals. However, the current production of Rh2 through the extraction of Panax ginseng is not sustainable and cannot meet the increasing market demand. Here, we increased the Rh2 production in Saccharomyces cerevisiae by engineering the heterologous xylose metabolic pathway. The Candida tropicalis derived xylose reductase-xylitol dehydrogenase pathway was successfully constructed in in the previously engineered protopanaxadiol (PPD)-producing strain. A PPD high-yield strain with strong xylose utilization capacity was optimized to reduce the generation of byproduct xylitol with the expression of a soluble pyridine nucleotide transhydrogenase. The new yeast chassis produced 494.0 mg/L PPD in xylose medium, which could provide sufficient precursors for Rh2. The Rh2 production strain was then constructed by integrating the glycosyltransferases UGTPg45 overexpression module and further optimized through systematic enhancement of its mevalonic acid (MVA) pathway, resulting in a Rh2 titer of 209.5 mg/L in xylose medium. In addition, we confirmed that the engineered strain also exhibited good fermentation performance in the lignocellulose hydrolysate mimic glucose-xylose mixed medium, and we further elucidated the reasons of higher Rh2 production in xylose fermentation stage through transcriptomic and metabolic analysis. Taken all strategies together, the resulting strain produced 1.47 g/L Rh2 in a 5 L bioreactor with mixed sugar medium, making it possible for a green and sustainable biotechnology to produce ginsenoside Rh2 using xylose efficiently. This is the first report on the use of xylose for high-value chemical ginsenoside Rh2 production, which will pave the way for the future industrial production of ginsenoside Rh2 from a renewable carbon source such as lignocellulose.

EPS Production by Lacticaseibacillus casei Using Glycerol, Glucose, and Molasses as Carbon Sources.

This study demonstrates that Lactobacillus can produce exopolysaccharides (EPSs) using alternative carbon sources, such as sugarcane molasses and glycerol. After screening 22 strains of Lactobacillus to determine which achieved the highest production of EPS based on dry weight at 37 °C, the strain Ke8 (L. casei) was selected for new experiments. The EPS obtained using glycerol and glucose as carbon sources was classified as a heteropolysaccharide composed of glucose and mannose, containing 1730 g.mol-1, consisting of 39.4% carbohydrates and 18% proteins. The EPS obtained using molasses as the carbon source was characterized as a heteropolysaccharide composed of glucose, galactose, and arabinose, containing 1182 g.mol-1, consisting of 52.9% carbohydrates and 11.69% proteins. This molecule was characterized using Size Exclusion Chromatography (HPLC), Gas chromatography-mass spectrometry (GC-MS), Fourier-transform infrared spectroscopy (FTIR), and proton nuclear magnetic resonance spectroscopy (1H-NMR). The existence of polysaccharides was confirmed via FT-IR and NMR analyses. The results obtained suggest that Lacticaseibacillus casei can grow in media that use alternative carbon sources such as glycerol and molasses. These agro-industry residues are inexpensive, and their use contributes to sustainability. The lack of studies regarding the use of Lacticaseibacillus casei for the production of EPS using renewable carbon sources from agroindustry should be noted.