This study demonstrates robot technology for harvesting edible bird’s nests within swiftlet houses. A comprehensive manipulator’s movement analysis of harvesting operation with a separating tool is provided for precisely collecting swiftlet nests. A robotic manipulator mounted on a mobile platform with a vision system is also analyzed and evaluated in this study. The actual harvesting or separating the swiftlet nests is performed with visual servo feedback. The manipulator performs the gross motions of separating tools and removing the nests under computer control with velocity and position feedback. The separating principle between the objective nest and wooden frame has been applied to a demonstration removal of nests using a four-degrees-of-freedom manipulator to perform the gross movements of tool. The actual separations using this system are accomplished as fast as the manipulator can be controlled to perform the necessary deceleration and topping at the end of separating. This is typically 2.0 s. This efficiency underscores the system’s capability for swift and precise operation in harvesting an edible bird nest task.