Removal Of Doxycycline Research Articles

Seeding-competent TDP-43 persists in human patient and mouse muscle.

TAR DNA binding protein 43 (TDP-43) is an RNA binding protein that accumulates as aggregates in the central nervous systems of some patients with neurodegenerative diseases. However, TDP-43 aggregation is also a sensitive and specific pathologic feature found in a family of degenerative muscle diseases termed inclusion body myopathy. TDP-43 aggregates from amyotrophic lateral sclerosis (ALS) and frontotemporal dementia brain lysates may serve as self-templating aggregate seeds in vitro and in vivo, supporting a prion-like spread from cell to cell. Whether a similar process occurs in patient muscle is not clear. We developed a mouse model of inducible, muscle-specific cytoplasmic localized TDP-43. These mice develop muscle weakness with robust accumulation of insoluble and phosphorylated sarcoplasmic TDP-43, leading to eosinophilic inclusions, altered proteostasis, and changes in TDP-43-related RNA processing that resolve with the removal of doxycycline. Skeletal muscle lysates from these mice also have seeding-competent TDP-43, as determined by a FRET-based biosensor, that persists for weeks upon resolution of TDP-43 aggregate pathology. Human muscle biopsies with TDP-43 pathology also contain TDP-43 aggregate seeds. Using lysates from muscle biopsies of patients with sporadic inclusion body myositis (IBM), immune-mediated necrotizing myopathy (IMNM), and ALS, we found that TDP-43 seeding capacity was specific to IBM. TDP-43 seeding capacity anticorrelated with TDP-43 aggregate and vacuole abundance. These data support that TDP-43 aggregate seeds are present in IBM skeletal muscle and represent a unique TDP-43 pathogenic species not previously appreciated in human muscle disease.

Biphenyl derived hyper-crosslinked polymer as a metal-free adsorbent for the removal of pharmaceuticals from water

The global concern of emergent aquatic pollutants, especially pharmaceutical contaminants, emphasizes the necessity for metal-free adsorbents to tackle water contamination issues. In this direction, a biphenyl-derived hyper-crosslinked polymer (poly-biph) was utilized as an adsorbent for the removal of ciprofloxacin (CPX) and doxycycline (DOX) from water. Micro-mesoporous hyper-crosslinked polymeric adsorbent (poly-biph) was synthesized by using biphenyl as precursor and formaldehyde dimethyl acetal (FDA) as crosslinker via microwave assisted method. It exhibits specific surface area (SABET) and pore volume of 1088 m2/g and 1.3 cm3/g, respectively. The spectral analysis confirmed the successful crosslinking of biphenyl with the linker FDA. The sorption efficiency of metal-free poly-biph for CPX and DOX was evaluated via batch and continuous flow modes under various operational parameters. poly-biph exhibited swift removal efficiency (>80 %) for CPX and DOX within a min. The batch mode sorption modeling revealed a chemisorption process and remarkable maximum sorption capacity of 470.8 and 425.1 mg/g for CPX and DOX, respectively. The continuous-flow studies of poly-biph revealed the better applicability of the Clark kinetic model with a maximum bed capacity of 313.5 and 372.4 mg/g for CPX and DOX, respectively. Synergistic mechanisms, including π-π interaction, electrostatic interaction, and pore-filling effect, were found to be the main driving forces for the sorptive removal of CPX and DOX onto poly-biph. The findings indicated that poly-biph possesses the ability to effectively eliminate DOX and CPX from the aquatic environment.

Characterization of immortalized bone marrow erythroid progenitor adult (imBMEP-A)—The first inducible immortalized red blood cell progenitor cell line derived from bone marrow CD71-positive cells

Background aimsEx vivo production of red blood cells (RBCs) represents a promising alternative for transfusion medicine. Several strategies have been described to generate erythroid cell lines from different sources, including embryonic, induced pluripotent, and hematopoietic stem cells. All these approaches have in common that they require elaborate differentiation cultures whereas the yield of enucleated RBCs is inefficient. MethodsWe generated a human immortalized adult erythroid progenitor cell line derived from bone marrow CD71-positive erythroid progenitor cells (immortalized bone marrow erythroid progenitor adult, or imBMEP-A) by an inducible expression system, to shorten differentiation culture necessary for terminal erythroid differentiation. It is the first erythroid cell line that is generated from direct reticulocyte progenitors and demonstrates robust hemoglobin production in the immortalized state. ResultsMorphologic analysis of the immortalized cells showed that the preferred cell type of the imBMEP-A line corresponds to hemoglobin-producing basophilic erythroblasts. In addition, we were able to generate a stable cell line from a single cell clone with the triple knockout of RhAG, RhDCE and KELL. After removal of doxycycline, part of the cells differentiated into normoblasts and reticulocytes within 5–7 days. ConclusionsOur results demonstrate that the imBMEP-A cell line can serve as a stable and straightforward modifiable platform for RBC engineering in the future.

Synthesis of reusable graphene oxide based nickel-iron superparamagnetic nanoadsorbent from electronic waste for the removal of doxycycline in aqueous media

Graphene oxide-based nickel-iron superparamagnetic nanoadsorbent (GO/Ni-Fe) was synthesized from electronic waste to effectively remove doxycycline (DXC) in aqueous media. The GO/Ni-Fe nanoadsorbent has been characterized using a number of instrumental techniques, including X-ray diffractometer, Zeta potential, Fourier transform infrared spectroscopy, elemental analyzer, vibrating sample magnetometer, transmission electron microscopy, energy dispersive x-ray, and X-ray photoelectron spectroscopy. These techniques showed that nickel-iron (Ni-Fe) nanoparticles with an average size of 4.26 nm were successfully fabricated on GO surfaces. The batch experiments were conducted under different conditions, including contact time, adsorption dosage, pH, concentration, and temperature, to determine the optimal conditions of the adsorption process. The maximum adsorption (90% removal) was established within 20 min, while the adsorbent dose was only 0.1 g/L at pH 5. The adsorption process was best fitted with the pseudo-second-order model, which suggests the interaction of doxycycline with the GO/Ni-Fe nanoadsorbent is mainly controlled by the chemisorption process. This may be due to hydrogen bonding as well as electrostatic interaction and π-π interaction between adsorbates and adsorbents. The isotherm data of the adsorption process was best fitted with Langmuir isotherm model with a maximum adsorption capacity of 13.02 mg g−1 at 25 °C, indicating that the adsorption is a monolayer adsorption to heterogeneous surfaces with electrostatic interaction. The superparamagnetic properties of GO/Ni-Fe nanoadsorbent can be easily separated by external magnetic field and regenerated with methanol washing. The findings unambiguously demonstrated that magnetically separable GO/Ni-Fe nanoadsorbent could be a good choice to remove DXC from wastewater sources.

Nitrogen-doped activated carbon derived from biomass waste for effective removal of doxycycline from aqueous solution: characterization and adsorption mechanism

Nitrogen-doped activated carbon derived from biomass waste for effective removal of doxycycline from aqueous solution: characterization and adsorption mechanism

Zn/Ce-layered double hydroxide for adsorptive removal of doxycycline from water

Since antibiotic overuse created various environmental issues, antibiotic management and separation are critical in ecological research. In this regard, the main objective of this work was to design a simple and reliable solvothermal-synthesis-based sorbent for removing antibiotics such as doxycycline (DOX). The used sorbent was Zn/Ce-layered double hydroxide (Zn/Ce-LDH), and its layered structure was confirmed by the X-ray diffraction (XRD), field emission scanning electron microscopy (SEM), transmission electron microscopy (TEM) analysis. In addition, the Brunauer-Emmett-Teller (BET) test showed the specific surface area of the Zn/Ce-LDH achieved 45.17 m2 g−1. The sorbent was shown to have a high capability to remove DOX (the maximum experimental adsorption capacity of 1297 mg g−1) at the low equilibrium contact time of 15 min. Also, the obtained negative value of Gibbs free energy (ΔG°), the enthalpy (ΔH°), and the entropy (ΔS°) at the investigated temperatures reveal the fact that the involved adsorption process had spontaneous and exothermic features. Besides, the pseudo-second-order kinetic model favourably described DOX adsorption on the Zn/Ce LDH surface. Furthermore, a combination of classical molecular dynamics and Monte Carlo simulations were utilized to investigate the adsorption properties of DOX on the (001) and (010) Zn/Ce-LDH surfaces. The theoretical findings supported the possibility of DOX coordination with Zn/Ce atoms.

Hydroxyapatite@cellulose@nZVI composite: Fabrication and adsorptive removal of doxycycline, Cr(VI) and As(III) from wastewater

In this study, a biogenic nanocomposite (HAP@CL@nZVI) was successfully developed using hydroxyapatite derived from eggshell waste, cellulose, and green-synthesized nano zero-valent iron for the removal of doxycycline (DOX), Cr(VI), and As(III) from aqueous solutions. Under optimized conditions, HAP@CL@nZVI demonstrated high adsorption capacity, with pH playing a critical role in the process with peak adsorption occurring at pH 8 for DOX, pH 2 for Cr(VI), and pH 7 for As(III). Mechanisms involved electrostatic attraction, hydrogen bonding, and π-π interactions for DOX, while electrostatic interactions and surface complexation governed Cr(VI) and As(III) adsorption. The Langmuir isotherm model indicated monolayer adsorption processes, with maximum adsorption capacities of 74.07, 111.11 and 79.37 mg/g for DOX, Cr(VI), and As(III) respectively. Kinetic studies unveiled pseudo-first-order kinetics for DOX and pseudo-second-order kinetics for Cr(VI) and As(III). The study highlights strong regeneration potential of HAP@CL@nZVI, rendering it a sustainable, eco-friendly adsorbent for pharmaceutical wastewater treatment and toxic metal ion remediation.

SOX4 reversibly induces phenotypic changes by suppressing the epithelial marker genes in human keratinocytes.

SOX4 is a transcription factor belonging to the SOX (Sry-related High Mobility Group [HMG] box) family and plays a pivotal role in various biological processes at various stages of life. SOX4 is also expressed in the skin in adults and has been reported to be involved in wound healing, tumor formation, and metastasis. In this study, we investigated the role of SOX4 in keratinocyte phenotypic changes. We generated a SOX4-overexpressing keratinocyte cell line that expresses SOX4 in a doxycycline (DOX)-inducible manner. DOX treatment induced a change from a paving stone-like morphology to a spindle-like morphology under microscopic observation. Comprehensive gene analysis by RNA sequencing revealed increased expression of genes related to anatomical morphogenesis and cell differentiation as well as decreased expression of genes related to epithelial formation and keratinization, suggesting that SOX4 induced EMT-like phenotype in keratinocytes. Differentially expressed genes (DEGs) obtained by RNA-seq were confirmed using qRT-PCR. DOX-treated TY-1 SOX4 showed a decrease in the epithelial markers (KRT15, KRT13, KRT5, and CLDN1) and an increase in the mesenchymal marker FN1. Protein expression changes by Western blotting also showed a decrease in the epithelial marker proteins keratin 15, keratin 13, and claudin 1, and an increase in the mesenchymal marker fibronectin. Removal of DOX from DOX-treated cells also restored the epithelial and mesenchymal markers altered by SOX4. Our results indicate that SOX4 reversibly induces an EMT-like phenotype in human keratinocytes via suppression of epithelial marker genes.

Ultrafast and simultaneous removal of four tetracyclines from aqueous solutions using waste material-derived graphene oxide-supported cobalt-iron magnetic nanocomposites.

In this work, a graphene oxide-supported cobalt-iron oxide (GO/Co-Fe) magnetic nanocomposite was successfully synthesized using waste dry cells for the efficient and simultaneous removal of tetracycline (TC), chlortetracycline (CTC), oxytetracycline (OTC), and doxycycline (DTC) from aqueous solutions. The GO/Co-Fe nanocomposite was thoroughly characterized using Fourier transform infrared spectroscopy, vibrating sample magnetometry, X-ray diffraction, field emission scanning electron microscopy, energy-dispersive X-ray spectroscopy, transmission electron microscopy, X-ray photoelectron spectroscopy, and zeta potential analysis. This multi-faceted characterization provided clean insights into the composition and properties of the synthesized nanocomposite. The adsorption of tetracyclines (TCs) was systematically investigated by assessing the influence of critical factors, such as adsorbent dosage, contact duration, initial pH of the solution, initial concentration, and temperature. The GO/Co-Fe adsorbent showed high removal efficiencies of 94.1% TC, 94.32% CTC, 94.22% OTC, and 96.94% DTC within 30 s contact period. The maximum removal efficiency of TCs was found at a low adsorbent dose of 0.15 g L-1. Notably, this superior removal efficiency was achieved at neutral pH and room temperature, demonstrating the adsorbent's efficacy under environmentally viable conditions. The kinetic studies demonstrated that the adsorption process was fitted satisfactorily with the pseudo-second-order model. Additionally, the adsorption behaviour of TCs on the GO/Co-Fe adsorbent was assessed by isotherm models, Langmuir and Freundlich. The experimental data followed the Langmuir isotherm, signifying a monolayer adsorption mechanism on the surface of the adsorbent. The adsorption capacities (qm) of GO/Co-Fe for TC, CTC, OTC and DTC were determined to be 64.10, 71.43, 72.46 and 99.01 mg g-1, respectively. Importantly, the GO/Co-Fe adsorbent showed reusability capabilities. The super magnetic properties of GO/Co-Fe made it easy to use for several cycles. These results clearly establish GO/Co-Fe as an exceptionally effective adsorbent for the removal of TCs from aqueous systems, highlighting its great potentiality in water treatment applications.

Serum biomarkers indicate sex differences in response to pathology inhibition in aged mice with tau pathology

AbstractBackgroundThe failure of some clinical trials using drugs that remove brain amyloid to prevent progression of dementia suggest that neurodegenerative disease may not progress in a linear fashion and threshold levels of one pathology may initiate alterations that are unable to be reversed upon removal of the initiating pathological process. We used the inducible Tg4510 mouse tauopathy model to determine whether neurodegeneration could be halted following removal of transgene expression in mice aged to 18 monthsMethodPathology was induced in mice from 8 weeks until 18 months of age (n = 20 equal male/female; both tau transgenic and non‐transgenic) by removal of doxycycline (dox) diet. Dox diet was returned to half the animals from 18‐23 months to inhibit pathology. Levels of serum Nfl were measured using SIMOA assay throughout disease progression and p‐tau181 along with other serum biomarkers were measured at endpoint. Immunohistochemical assays were performed to quantitate numbers of neurons (NeuN) and tau load (PHF‐tau) in hippocampus.ResultSerum Nfl increased over aging in all mice and was significantly higher in tau transgenics relative to non‐transgenics at 18 months (p<0.05). Between 18 and 23 months, the rate of serum Nfl increase was significantly(p<0.05) reduced in male transgenic mice on dox compared to female transgenic mice, so that by 23 months levels were not different from non‐transgenic mice. Similarly, the numbers of neurons in the hippocampus of male mice on dox at 23 months was significantly higher than their female counterparts (p<0.05). When looking at levels of serum p‐tau181 at 23 months, there was significantly (p<0.05) higher levels in male mice on dox than female miceConclusionThese data suggest that aged female mice have ongoing neurodegeneration following removal of pathology induction that is similar to mice with continued pathology induction. The differences in serum p‐tau‐181 may indicate that female mice fail to clear pathological tau from the brain. Further studies will examine the relationship between histopathology and blood biomarkers and determine whether female mice on dox have a higher tau pathology load than their male counterparts.

Doxycycline Removal by Solar Photo-Fenton on a Pilot-Scale Composite Parabolic Collector (CPC) Reactor

In this study, the solar photo-Fenton (SPF) process was investigated for the degradation of doxycycline (DOX) using a solar compound parabolic collector (CPC) reactor and a borosilicate serpentine tube with an irradiated volume of 1.8 L. The influence of the operating parameters, such as H2O2, Fe2+ dosage, and DOX concentration, was investigated. The optimum H2O2, Fe2+ dosage, and DOX concentration were found to be 4, 0.1, and 0.06 mM, respectively. The results of photo-Fenton experiments fitted the pseudo-first-order kinetic equation (R2 = 0.99). The efficiency of the treatment under optimized conditions was analyzed by an HPLC analysis of the samples, chemical oxygen demand (COD), and total organic carbon (TOC). The results obtained showed that the solar photo-Fenton process achieved a DOX degradation of 95.07%, a COD elimination of 81.43%, and a TOC elimination of 73.05%. The phytotoxicity tests revealed a 73.32% decrease in the germination index of watercress seeds, demonstrating that the SPF process minimizes the toxicity of the chemical and did not have any negative impact on plant growth. Overall, the results of this study suggest that SPF is a promising technology for the removal of doxycycline from wastewater.

Simultaneous removal of microplastics and doxycycline and preparation of novel hollow carbon nanocakes by pyrolysis

Microplastics (MPs) pose a significant challenge in terms of their safe disposal and effective utilization. This study aimed to address this issue by employing coagulation and pyrolysis to achieve the simultaneous removal of MPs and doxycycline (DC). Through the addition of nano micro-electrolysis material (nMET) prior to poly ferric sulfate (PS) and polyacrylamide (PAM) coagulation, 92% of DC and 96% of MPs in wastewater were successfully removed. The resulting product from this process is a new type of hollow carbon nanocakes (HCNCs) obtained through pyrolysis. Characterization of the HCNCs reveals that they consist of amorphous carbon, ferric oxide, and zero-valent iron, with a diameter, thickness, specific surface area, and ID/IG of approximately 350 nm, 50 nm, 54.37 m2g−1, and 0.92, respectively. The cavity structure of the HCNCs is formed during the pyrolysis process as CO2 escapes between interstitial compounds. This research not only expands the potential applications of nMET in the environmental field but also presents a method for producing new iron-carbon materials by utilizing waste MPs.

Efficient removal of doxycycline by MnNb2O6/sulfite process: Response surface methodology optimization and mechanism analysis

Sulfate radical-based advanced oxidation process could efficiently remove many emerging contaminants from wastewater. Considering the unique properties of MnNb2O6, an efficient MnNb2O6/sulfite system was developed to remove doxycycline residue from the aqueous environment. In this research, MnNb2O6 nanomaterial was successfully synthesized through a one-step hydrothermal route at 260 °C for 24 h. The paramagnetic behavior of MnNb2O6 was observed at 300 K. Scanning electron microscopy and transmission electron microscopy images showed that the size of petal-like MnNb2O6 crystals was 250–700 nm. The specific surface area of MnNb2O6 was 24.84 m2/g. Optimization of MnNb2O6/sulfite process was achieved by Box-Behnken Design based on response surface methodology. Under the predicted optimal conditions (pH of 8.7, MnNb2O6 dosage of 0.29 g/L, doxycycline concentration of 10.8 mg/L, and sodium sulfite concentration of 4.3 mM), the degradation efficiency of doxycycline could reach 85.1%. Mn species was found to be the dominant factor in MnNb2O6/sulfite system. Both SO4•− and •OH were identified as primary active radicals in MnNb2O6/sulfite system. Furthermore, six transformation products of doxycycline were elucidated. This work offers a simple water treatment process for efficiently removing doxycycline residue in the environment.

Enhanced Removal of Doxycycline by Simultaneous Potassium Ferrate(VI) and Montmorillonite: Reaction Mechanism and Synergistic Effect

Doxycycline (DOX), a typical antibiotic, is harmful to aquatic ecosystems and human health. This study presents DOX removal by potassium ferrate (Fe(VI)) and montmorillonite and investigates the effect of Fe(VI) dosage, reaction time, initial pH value, montmorillonite dosage, adsorption pH, time and temperature on DOX removal. The results show that DOX removal increases when increasing the Fe(VI) dosage, with the optimal condition for DOX removal (~97%) by Fe(VI) observed under a molar ratio ([Fe(VI)]:[DOX]) of 30:1 at pH 7. The reaction of DOX with Fe(VI) obeyed second-order kinetics with a rate constant of 10.7 ± 0.45 M−1 s−1 at pH 7. The limited promotion (~4%) of DOX adsorption by montmorillonite was observed when the temperature increased and the pH decreased. Moreover, the synergetic effect of Fe(VI) and montmorillonite on DOX removal was obtained when comparing the various types of dosing sequences (Fe(VI) oxidation first and then adsorption; adsorption first and then Fe(VI) oxidation; simultaneous oxidation and adsorption). The best synergistic effect of DOX removal (97%) was observed under the simultaneous addition of Fe(VI) and montmorillonite, maintaining the Fe(VI) dosage (from 30:1 to 5:1). Five intermediates were detected during DOX degradation, and a plausible DOX degradation pathway was proposed.

The Use of a Novel Graphitic Carbon Nitride/Cobalt Molybdate (G-C3 n4 /ComoO4 ) Nanocomposites for the Doxycycline Removal by Photocatalytic Degradation in Pharmaceutical Industry Wastewaters and the Evaluation of Microtox (Aliivibrio Fischeri) and Daphnia Magna Acute Toxicity Assays

In this study, a novel graphitic carbon nitride/cobalt molybdate (g-C3 N4 /CoMoO4 ) nanocomposites (NCs) as a photocatalys was examined during photocatalytic degradation process in the efficient removal of Doxycycline (DOX) from pharmaceutical industry wastewater plant, İzmir, Turkey. Different pH values (3.0, 4.0, 6.0, 7.0, 9.0 and 11.0), increasing DOX concentrations (5 mg/l, 15 mg/l, 30 mg/l and 45 mg/l), increasing g-C3 N4 /CoMoO4 NCs concentrations (1 mg/l, 2 mg/l, 3 mg/l, 4 mg/l, 6 mg/l, 8 mg/l and 10 mg/l), different g-C3 N4 /CoMoO4 NCs mass ratios (5/5, 6/4, 7/3, 8/2, 9/1, 1/9, 2/8, 3/7 and 4/6), increasing recycle times (1., 2., 3., 4., 5., 6. and 7.) was operated during photocatalytic degradation process in the efficient removal of DOX in pharmaceutical industry wastewater. The characteristics of the synthesized nanoparticles (NPs) were assessed using X-Ray Difraction (XRD), Field Emission Scanning Electron Microscopy (FESEM), Energy-Dispersive X-Ray (EDX), Fourier Transform Infrared Spectroscopy (FTIR), Transmission Electron Microscopy (TEM), and Diffuse reflectance UV-Vis spectra (DRS) analyses, respectively. The acute toxicity assays were operated with Microtox (Aliivibrio fischeri also called Vibrio fischeri) and Daphnia magna acute toxicity tests. ANOVA statistical analysis was used for all experimental samples. The maximum 99% DOX removal efficiency was obtained during photocatalytic degradation process in pharmaceutical industry wastewater, at 150 W UV-vis light irradiation power, after 180 min photocatalytic degradation time, at pH=9.0 and at 25°C, respectively

Bio-Electrochemical Cell Assisted Production of Biogenic Palladium Nanoparticles with Shewanella oneidensis MR-1 Bacteria for the Catalytic Removal of Ofloxacin (OFX) and Doxycycline (DOX) Micropollutants in Pharmaceutical Industry Wastewaters

In this study, biogenic-palladium nanoparticles (bio-Pd NPs) with Shewanella oneidensis MR-1 bacteria as a heterostructure bio-electrochemical cell catalyts was examined during catalytic degradation process in the efficient removal of Ofloxacin (OFX) and Doxycycline (DOX) micropollutants from pharmaceutical industry wastewater plant, İzmir, Turkey. Different pH values (3.0, 4.0, 6.0, 7.0, 9.0 and 11.0), increasing micropollutants (OFX and DOX) concentrations (5 mg/l, 15 mg/l, 30 mg/l and 45 mg/l), increasing Bio-Pd NPs concentrations (5 mg/l, 10 mg/l, 20 mg/l, 30 mg/l, 40 mg/l and 60 mg/l), different Bio-Pd NPs/cell dry weight (CDW) mass ratios (5/5, 6/4, 7/3, 8/2, 9/1, 1/9, 2/8, 3/7 and 4/6), increasing recycle times (1., 2., 3., 4., 5., 6. and 7.) was operated during catalytic degradation process in the efficient removals of OFX and DOX micropollutants in pharmaceutical industry wastewater. The characteristics of the synthesized NPs were assessed using Diffuse reflectance UV-Vis spectra (DRS), Energy-dispersive X-ray (EDX), Field emission scanning electron microscopy (FESEM), Fourier transform infrared spectroscopy (FTIR), Inductively coupled plasma mass spectrometry (ICP-MS), Transmission Electron Microscopy (TEM), X-Ray Diffraction (XRD) and X-Ray Photoelectron Spectroscopy (XPS) analyses, respectively. The catalytic activity was first assessed by the degradation of methyl orange. The Bio-NPs showing the highest catalytic activity were selected for the removal of micropollutants (OFX and DOX) from secondary treated municipal wastewater. The catalytic degradation mechanisms of bio-Pd NPs with Shewanella oneidensis MR-1 bacteria as a heterostructure bio-electrochemical cell catalysts and the reaction kinetics of OFX and DOX micropollutants were evaluated in pharmaceutical industry wastewater during catalytic degradation process. ANOVA statistical analysis was used for all experimental samples. The maximum 99% OFX removal efficiency was obtained catalytic removals with bio-electrochemical cell assisted production of bio-Pd NPs with Shewanella oneidensis MR-1 bacteria bio-electrochemical cell catalyts in pharmaceutical industry wastewater, at 30 mg/l OFX concentration, 40 mg/l Bio-Pd NPs concentration, Bio-Pd NPs/CDW mass ratio=6/4, after 24 h catalytic degradation time, at pH=6.0, at 25oC, respectively. The maximum 99% DOX removal efficiency was observed catalytic removals with bio-electrochemical cell assisted production of bio-Pd NPs with Shewanella oneidensis MR-1 bacteria bio-electrochemical cell catalyts in pharmaceutical industry wastewater, at 30 mg/l DOX concentration, 40 mg/l Bio-Pd NPs concentration, Bio-Pd NPs/CDW mass ratio=6/4, after 24 h catalytic degradation time, at pH=9.0, at 25oC, respectively. Finally, the combination of a simple, easy operation preparation process, excellent performance and cost effective, makes this Bio-Pd NPs with Shewanella oneidensis MR-1 bacteria bio-electrochemical cell catalyts a promising option during catalytic degradation process in pharmaceutical industry wastewater treatment.

Peripheral vs. core body temperature as hypocretin/orexin neurons degenerate: Exercise mitigates increased heat loss

Hypocretins/Orexins (Hcrt/Ox) are hypothalamic neuropeptides implicated in diverse functions, including body temperature regulation through modulation of sympathetic vasoconstrictor tone. In the current study, we measured subcutaneous (Tsc) and core (Tb) body temperature as well as activity in a conditional transgenic mouse strain that allows the inducible ablation of Hcrt/Ox-containing neurons by removal of doxycycline (DOX) from their diet (orexin-DTA mice). Measurements were made during a baseline, when mice were being maintained on food containing DOX, and over 42 days while the mice were fed normal chow which resulted in Hcrt/Ox neuron degeneration. The home cages of the orexin-DTA mice were equipped with running wheels that were either locked or unlocked. In the presence of a locked running wheel, Tsc progressively decreased on days 28 and 42 in the DOX(-) condition, primarily during the dark phase (the major active period for rodents). This nocturnal reduction in Tsc was mitigated when mice had access to unlocked running wheels. In contrast to Tsc, Tb was largely maintained until day 42 in the DOX(-) condition even when the running wheel was locked. Acute changes in both Tsc and Tb were observed preceding, during, and following cataplexy. Our results suggest that ablation of Hcrt/Ox-containing neurons results in elevated heat loss, likely through reduced sympathetic vasoconstrictor tone, and that exercise may have some therapeutic benefit to patients with narcolepsy, a disorder caused by Hcrt/Ox deficiency. Acute changes in body temperature may facilitate prediction of cataplexy onset and lead to interventions to mitigate its occurrence.

Efficient removal of doxycycline using Schwertmannite as a heterogeneous Fenton-like catalyst over a wide pH range

Residual doxycycline (DOX) in the aquatic environment has received a considerable amount of critical attention due to its toxicity and widespread distribution in the environment. This study explored a simplified and rapid method to remove DOX over a wide pH range (pH = 2–10). Schwertmannite (SCH) was synthesized using biological methods to remove DOX in combination with H2O2. The results showed that 98.52% of DOX and 57.35% of total organic carbon (TOC) was removed over 30 min at 50 ºC without any external energy input, where the addition of SCH and H2O2 was 1.6 g/L and 71 mg/L, respectively. Meanwhile, the DOX removal efficiency was 98.18% at 50 ºC over 30 min and 97.70% of DOX could even be removed at 10 ºC when the reaction time was prolonged to 120 min. Hydroxyl radicals (·OH) were the main oxidative species. Moreover, the removal efficiencies of DOX after 3 h in the SCH/H2O2 system were 26.97%, 31.54%, 39.23%, 47.07%, 68.43%, 88.92%, and 97.64% in actual aquaculture wastewater when the COD concentrations was 7000, 5000, 3000, 1000, 500, 200, and 100 mg/L, respectively. The results of this study provided a simple and rapid method for removing DOX from DOX-containing wastewater.

The effect of Fe–Zn mole ratio (2:1) bimetallic nanoparticles supported by hydroxyethyl cellulose/graphene oxide for high-efficiency removal of doxycycline

In this research, Hydroxyethyl cellulose – graphene oxide HEC-GO and HEC-GO/Fe–Zn mole ratio (2:1) nanocomposite as adsorbents were fabricated by crosslinking ethylene glycol dimethacrylate (EGDMA) to study the thermodynamic, kinetic and isotherm of doxycycline antibiotic adsorption. The morphology and structure of the adsorbents were analyzed by Fourier transform infrared spectroscopy (FT-IR), Field Emission Scanning Electron Microscopy with Energy Dispersive X-Ray Spectroscopy (FE-SEM- EDX), and Transmission electron microscopy (TEM). The adsorption behavior of doxycycline (DOX) was studied with different parameters including doxycycline concentration, pH, the dose of adsorbent (HEC-GO and HEC-GO/Fe–Zn, mole ratio (2:1)), contact time, and temperature. The optimal conditions for the removal of DOX are pH = 3.0, contact time 100 min, and 20 min for HEC-GO and HEC-GO/Fe–Zn mole ratio (2:1). The removal percentage for HEC-GO and HEC-GO/Fe–Zn mole ratio (2:1) was 97% and 95.5%, respectively. Equilibrium adsorption isotherms such as the Langmuir, Freundlich, and Temkin models were analyzed according to the experimental data. Also, four adsorption kinetics were investigated for removing DOX. The Langmuir isotherm and pseudo-second-order kinetic models provided the best fit for experimental data for HEC-GO and HEC-GO/Fe–Zn mole ratio (2:1). Thermodynamic data showed that negative values of Gibbs free energy (ΔG°) and the negative value of enthalpy (ΔH°) of the adsorption process for adsorbents. It means that DOX removal was a spontaneous and exothermic reaction.

Synergetic effect of green synthesized reduced graphene oxide and nano-zero valent iron composite for the removal of doxycycline antibiotic from water

In this work, the synthesis of an rGO/nZVI composite was achieved for the first time using a simple and green procedure via Atriplex halimus leaves extract as a reducing and stabilizing agent to uphold the green chemistry principles such as less hazardous chemical synthesis. Several tools have been used to confirm the successful synthesis of the composite such as SEM, EDX, XPS, XRD, FTIR, and zeta potential which indicated the successful fabrication of the composite. The novel composite was compared with pristine nZVI for the removal aptitude of a doxycycline antibiotic with different initial concentrations to study the synergistic effect between rGO and nZVI. The adsorptive removal of bare nZVI was 90% using the removal conditions of 25 mg L−1, 25 °C, and 0.05 g, whereas the adsorptive removal of doxycycline by the rGO/nZVI composite reached 94.6% confirming the synergistic effect between nZVI and rGO. The adsorption process followed the pseudo-second order and was well-fitted to Freundlich models with a maximum adsorption capacity of 31.61 mg g−1 at 25 °C and pH 7. A plausible mechanism for the removal of DC was suggested. Besides, the reusability of the rGO/nZVI composite was confirmed by having an efficacy of 60% after six successive cycles of regeneration.