Release calls are a non-receptive response to amplexus between different species or conspecific amplexus between males. This call differs between closely related species and can be an important tool for the taxonomy of this group. Here, we describe, for the first time, the release call of Dendropsophus sanborni (Schmidt, 1944) from a record during a field trip in a palm grove in southern Brazil. We recorded two release calls from a male individual of D. sanborni while handling it. These release calls are simply formed by one note with no modulation. Release calls of Dendropsophus are poorly reported in the literature, and comparisons among them are limited. Release calls are possibly much more common than reported in the literature and more description studies are needed.