Release Behavior Of Nanoparticles Research Articles

Fabrication and evaluation of poly (vinyl alcohol)/gelatin fibrous scaffold containing ZnO nanoparticles for skin tissue engineering applications

Skin engineering offers innovative constructs for the treatment of a wide range of skin diseases. Hybrid scaffolds were fabricated using poly (vinyl alcohol), gelatin and ZnO nanoparticles by electrospinning. Different concentrations of ZnO nanoparticles (0%, 0.5% and 1%) were incorporated into the PVA/gelatin scaffolds and the constructs were characterized by SEM, FTIR, XRD, contact angle, swelling, degradation, and biomechanical tests; the release behavior of nanoparticles from the scaffolds was also evaluated. In cellular studies, human mesenchymal stem cells (hu-MSCs) were extracted from the umbilical cord of a newborn baby and characterized by flow cytometry. Cell-seeded scaffolds were assessed by in vitro biocompatibility, SEM images and live/dead assay. Finally, the antibacterial activity of scaffolds against E. coli and S. aureus strain was determined. Due to the presence of the hydrophilic biopolymer of gelatin in the contact angle test, the scaffolds showed hydrophilic properties. With the addition of nanoparticles, the hydrophilicity, water absorption and degradation rates of scaffolds were somewhat reduced. No difference in tensile modulus was observed between scaffolds. The release rate of nanoparticles from scaffolds increased over time. The immunoprofile evaluation of extracted cells by flow cytometry technique confirmed the mesenchymal nature of the cells. Fluorescent staining showed that most cells were alive and had appropriate shape during six days of cell culture on PVA/gelatin/ZnO1wt% scaffold. All scaffolds had acceptable biocompatibility, but control and PVA/gelatin groups showed a higher cell survival rate. Scaffolds containing nanoparticles had a direct effect on inhibiting bacterial growth in a dose-dependent manner. Our findings suggest that composite scaffolds containing ZnO nanoparticles seeded with hu-MSCs might be suitable for promoting skin healing.

Donepezil loaded PLGA Nanoparticles, from Modified Nano-Precipitation, an Advanced Drug Delivery System to treat Alzheimer Disease

In present works we synthesized Donepezil loaded PLGA nanoparticles (NPs). The approach of our research group was to prove the improvement of drug transport through the Blood Brain Barrier when donepezil was loaded with PLGA. It promoted the uptake of drug into brain endothelium compared with the free drug and play a significant role in the treatment of Alzheimer’s disease (AD). The NPs were synthesized by modified Nano precipitation method. These synthesized polymeric nanoparticles were characterized for particle size, Polydispersity index (PDI) and Zeta potential. The average size and PDI of drug loaded polymeric nanoparticle for preferred formulation were found to be 40.8 nm and 0.188 respectively. The Entrapment Efficiency was 74% and Process yield was 78%. The electron microscopic images of polymeric nanoparticles suggested that the particles were spherical in shape. The pharmacokinetics showed that the release behavior of NPs were very much similar to sustained release and follow Hixson Crowell model.

Comparison of two nanocarriers for quercetin in morphology, loading behavior, release kinetics and cell inhibitory activity

Recently, nanocarrier loading drugs have caused widespread concern. However, there are few reports about comparison of drug loading behavior of different nanoparticles. In this study, we prepared two nanocarriers (starch nanoparticles and F127 nanoparticles) for oral and intravenous injections, respectively. We used dynamic light scattering and transmission electron microscopy to study the morphology of the nanocarriers. Moreover, the loading behavior of nanoparticles on quercetin (QC) and the release behavior of nanoparticles at different pH levels were studied. In artificial gastric (pH 2.0) and intestinal (pH 6.8) juices, QC showed a sustained release in two nanoparticles. Meanwhile starch nanoparticle-QC and F127-QC nanosystems both showed relatively higher inhibitory activities than free QC on five kinds of cells, especially for cell A549, the cell inhibition ratio achieved to 55.16% and 64.06%, respectively. This study provided a reference for oral and injection of drug-loaded nanoparticles.

Acidity and Glutathione Dual-Responsive Polydopamine-Coated Organic-Inorganic Hybrid Hollow Mesoporous Silica Nanoparticles for Controlled Drug Delivery.

Controversial biodegradability and nonspecific pre-drug leakage are major limitations for inorganic nanoparticles in cancer treatment. To solve these problems, we developed organic-inorganic hybridized hollow mesoporous silica nanoparticles with polydopamine modifications on the surface to simultaneously achieve enhanced biodegradability and controllable drug release. The morphology and chemical structure of the nanoparticles were characterized by TEM, N2 adsorption-desorption isotherms, TEM-mapping and XPS. Moreover, the release behavior of nanoparticles under various pH conditions and the degradation behavior in the presence of GSH were evaluated. With effective controlled release, HMONs-PTX@PDA were shown to significantly inhibit cancer cell proliferation and achieve antitumor effects in vivo through dual-response release in the tumor microenvironment. Overall, this nanoplatform has significant potential to achieve tumor microenvironment-responsive degradation and release to enhance tumor accumulation, which is very promising for cancer treatment.

Self-aggregation behavior of hydrophobic sodium alginate derivatives in aqueous solution and their application in the nanoencapsulation of acetamiprid

In this study, cholesteryl-grafted sodium alginate derivatives (CSAD) with different molecular weights were synthesized by esterification. The structure of CSAD was confirmed by FT-IR and 1H NMR spectrometers. The effects of pH and CSAD polymer concentration on the self-assembled behavior and particle size of CSAD were investigated by fluorescence measurement (FM) and dynamic light scattering (DLS). In the presence of Ca2+, the cholesteryl-grafted sodium alginate derivative was used for fabricating self-assembled nanoparticles that can effectively encapsulate the drug acetamiprid. The drug-loaded nanoparticles were characterized by transmission electron microscopy (TEM). The encapsulation efficiency (EE) and acetamiprid drug release behavior from the nanoparticles were also studied. The results reveal that CSAD self-assembled nanoparticles had a diameter of 100nm and were nonaggregated in aqueous media; Moreover, the encapsulation efficiency and the release behavior of nanoparticles were influenced by the MW of CSAD. The mechanism of acetamiprid release was found to vary from non-Fickian (anomalous) to Fickian transport with a decrease in the molecular weight of CSAD.