PurposeThe paper aims to investigate the dynamic measurement of the water vapour resistance. The water vapour diffusion kinetics depends on the fibre’s material. So, water vapour resistance measurement times till the equilibrium steady state can vary in the case of natural fibres compared to synthetic fibres. Devices for determining water vapour resistance according to the ISO 11092 standard allow static values to be measured.Design/methodology/approachIn this study to investigate the dynamic of the water vapour resistance, a new parameter named “holding period” was introduced and defined as the time from sample placement on the measuring head until the measuring process begins. The holding period was varied as 0, 30, 60, 90, 120, 180, 240 and 300 s. Wool and cotton knitted fabrics were tested as natural fibres and compared to 100% polyester and 90% polyester/10% elastane as synthetic fibres. Measurements were conducted under both air velocities of 1 and 2 m/s. The experimental test data were statistically analysed based on ANOVA and four-in-one residual plots.FindingsStatistical analysis of experimental tests shows that the holding period affects water vapour resistance in both air velocities of 1 and 2 m/s and on the measured values in the case of hydrophilic fibres.Research limitations/implicationsThe study of the dynamic relative water vapour permeability of natural and synthetic is an important area of interest for future research.Practical implicationsIt is recommended to hold the samples on the top of the head measurement before starting the test.Originality/valueFollowing the ISO 11092 standard, the static values of the water vapour resistance were measured without considering the dynamic behaviour of the water vapour diffusion through the textile fabrics. This paper fulfils an experimental dynamic measurement of the water vapour resistance.