Relative Abundances Of Elements Research Articles

Mineralization of Zr-REE-Y in the Ngaoumbol iron formations, central Cameroon: Insights from petrography, mineral chemistry and whole rock geochemistry

The increasing importance of rare earth elements (REE) and critical metals in contemporary society has led us to investigate the mineral potential of the Ngaoumbol area, located within Cameroon's Central African Fold Belt (CAFB). In this study, we have conducted a comprehensive analysis that includes petrography, whole rock geochemistry, and mineral chemistry, along with the application of an outlook coefficient known as Koult, with the aim to evaluate the prospectivity of the Ngaoumbol iron formations as a potential source of REE-Y resources. This coefficient is defined as the ratio of the relative abundance of critical Rare Earth Elements to the relative abundance of excess REE. The iron formations in the Ngaoumbol area are fine-to medium-grained foliated rocks with alternating magnetite and actinolite bands and quartz bands, suggesting a sedimentary parentage. These rocks have an average REE-Y content of 1438.43 ppm and a Koult of 0.77, indicating their potential as raw sources for REE. Furthermore, the investigated samples exhibit high average Zr content (7748 ppm), suggesting that the rocks may host potentially economic Zr ore. The Zr-REE-Y mineralization in the Ngaoumbol area is hosted in detrital zircon, monazite and xenotime, probably deriving from the weathering of alkaline/subalkaline rocks surrounding the deposits. Our findings suggest that the Ngaoumbol area has promising REE and Zr resources. However, further exploration and evaluation are necessary to determine the extent and economic viability of these resources.

Mantle Sources and Geochemical Evolution of the Picture Gorge Basalt, Columbia River Basalt Group

The Columbia River Basalt Group (CRBG) is the youngest continental flood basalt province, proposed to be sourced from the deep-seated plume that currently resides underneath Yellowstone National Park. If so, the earliest erupted basalts from this province, such as those in the Picture Gorge Basalt (PGB), aid in understanding and modeling plume impingement and the subsequent evolution of basaltic volcanism. Using geochemical and isotopic data, this study explores potential mantle sources and magma evolution of the PGB. Long known geochemical signatures of the PGB include overall large ion lithophile element (LILE) enrichment and relative depletion of high field strength elements (HFSE) typical of other CRBG main-phase units. Basaltic samples of the PGB have 87Sr/86Sr ratios on the low end of the range displayed by other CRBG lavas and mantle-like δ18O values. The relatively strong enrichment of LILE and depletion of HFSE coupled with depleted isotopic signatures suggest a metasomatized upper mantle as the most likely magmatic source for the PGB. Previous geochemical modeling of the PGB utilized the composition of two high-MgO primitive dikes exposed in the northern portion of the Monument Dike swarm as parental melt. However, fractionation of these dike compositions cannot generate the compositional variability illustrated by basaltic lavas and dikes of the PGB. This study identifies a second potential parental PGB composition best represented by basaltic flows in the extended spatial distribution of the PGB. This composition also better reflects the lowest stratigraphic flows identified in the previously mapped extent of the PGB. Age data reveal that PGB lavas erupted first and throughout eruptions of main-phase CRBG units (Steens, Imnaha, Grande Ronde Basalt). Combining geochemical signals with these age data indicates cyclical patterns in the amounts of contributing mantle components. Eruption of PGB material occurred in two pulses, demonstrated by a ~0.4 Ma temporal gap in reported ages, 16.62 to 16.23 Ma. Coupling ages with observed geochemical signals, including relative elemental abundances of LILE, indicates increased influence of a more primitive, potentially plume-like source with time.

Natural radioactivity of the crust as an important factor of the chemical evolution of the early Earth

The long-lived (billions of years) 40K, 235U, 238U, and 232Th isotopes have a high energy capacity and represent a potent internal source of energy. Together with the external action of the Sun, the natural radioactivity was apparently an important component of evolution of the early Earth and development of Earth's life. The decay of isotopes initiated radiation chemical reactions involving water, which were accompanied by the formation of hydrogen and oxygen and the transformation of Earth's inorganic matter into organic compounds, including prebiotic molecules. Water radiolysis in the Earth's crust continuously produces H2, which is now considered as the main electron donor (food) for microorganisms several kilometers below the Earth's surface. Here we calculate the initial radioactivity of the early Earth's crust from the relative abundances of elements and dynamics of variation of the radioactivity and energy release upon the decay of particular radioactive isotopes. The energy released upon the decay of heavy 235U, 238U, and 232Th throughout the existence of the Earth (4.6 billion years) was 4.5 × 1030 J, while the energy released upon the decay of 40K was approximately 1.0 × 1030 J. The energy release per year during the first billion years decreased from 3.0 × 1021 J to 1.5 × 1021 J for heavy isotopes and from 0.70 × 1021 J to 0.45 × 1021 J for 40K. Over the last billion years, the energy decreased approximately threefold. Analysis of the data on the radiation chemical formation of hydrogen in the wet components of the Earth's crust (cements, zeolites, geopolymers, and other) and bottom sediments indicates that hydrogen is formed upon direct action of radiation on water in yields characteristic of pure water. According to calculations, over the past 4 billion years, approximately 1.7 × 1022 M of hydrogen was formed. Furthermore, ∼93 % of H2 was due to the decay of uranium and thorium, while the contribution of 40K did not exceed 7 %. The global rate of hydrogen formation in the Earth's crust is ∼ 1.5 × 1012 mol per year. A mechanism involving ionic and radical products of water radiolysis was proposed to describe the formation of prebiotic molecules (amino acids, sugars, etc.) from carbon-, nitrogen-, and sulfur-containing inorganic compounds.

The genomic study of repetitive elements in Solea senegalensis reveals multiple impacts of transposable elements in the evolution and architecture of Pleuronectiformes chromosomes

Pleuronectiformes are flatfishes with high commercial value and a prominent example of successful marine adaptation through chromosomal evolution. Hence, the aim of this study was to analyze the 14 relative abundance of repetitive elements (satellite DNA and transposable elements (TE)) in the 15 genome of 10 fish species (8 flatfish) delving into the study of the species of special relevance, 16 Senegalese sole, Solea senegalensis. The results showed differences in the abundance of repetitive elements, with S. senegalensis exhibiting the highest frequency and coverage of these elements reaching the 40% of the genome and not at random distribution. It is noteworthy the presence of relevant peaks of Helitrons in centromeric/pericentromeric positions mainly in the bi-armed chromosomes 1, 2, 4, 6, 7, and 9. The position of the centromeres of this species determined through the genomic localization of the family of satellite DNA PvuII, and other repetitive sequences was obtained de novo. This allowed us to know the genomic position of the centromeres in 19 out of the 21 chromosomes of S. senegalensis. Helitrons showed an accumulation of tandem copies mainly in the pericentromeric positions of chromosomes 1 and 2, occupying a region, in the first case, of 600Kb of tandem repeats. That has only been previously described in mammals and plants. Divergence and copy number studies indicated the presence of active families in the species’ genome and the existence of two important events of transposon activity (burst) in the genome of S. senegalensis, mainly accentuated in Helitrons. The results showed that only the families of DNA transposons exhibited a landscape with symmetrical bell-shaped distribution. The phylogenetic analysis of Helitron families revealed the presence of two large groups of families and the presence of four groups of sequences with heterogeneous distribution among chromosomes. Finally, the phylogenomic analysis of 8615 sequences belonging to Helitron insertions from 5 families of flatfish and two external species, allowed to classify the copies into nine groups of sequences with different levels of divergence and clusters, including some branches with distant phylogenetically species. The implications of this study will help to expand the knowledge of chromosome structure and evolution of these species.

The infalling elliptical galaxy M89: the chemical composition of the AGN disturbed hot atmosphere

ABSTRACT The chemical enrichment of X-ray-emitting hot atmospheres has hitherto been primarily studied in galaxy clusters. These studies revealed relative abundances of heavy elements that are remarkably similar to Solar. Here, we present measurements of the metal content of M89 (NGC 4552), an elliptical galaxy infalling into the Virgo cluster with a ∼10 kpc ram-pressure stripped X-ray tail. We take advantage of deep Chandra and XMM–Newton observations, and with particular attention to carefully modelling the spectra, we measure the O/Fe, Ne/Fe, Mg/Fe, Si/Fe, and S/Fe ratios. Contrary to previous measurements in galaxy clusters, our results for the hot atmosphere of M89 suggest super-Solar abundance ratios with respect to iron (i.e. α/Fe > 1), similar to its stellar components. Our analysis of the active galactic nucleus (AGN) activity in this system indicates that the AGN-induced outflow could have facilitated the stripping of the original galactic atmosphere, which has been replaced with fresh stellar mass-loss material with super-Solar α/Fe abundance ratios. Additionally, we report a new fitting bias in the RGS data of low-temperature plasma. The measured O/Fe ratios are >1σ lower in multitemperature models than a single temperature fit, leading to discrepancies in the calculations of supernova fractions derived from the metal abundances.

Interpretation of High-Resolution Airborne Radiometric Data Over Part of Lower Benue Trough, Nigeria

Airborne radiometric data of part of Lower Benue Trough of Nigeria were interpreted quantitatively to determine the relative abundance of natural radioactive elements of Uranium (U), Thorium (Th) and Potassium (K) in the area. The observed count rate for Uranium, Thorium and Potassium in the study area were 0.8−6.8 ppm, 4.3−23.2 ppm and 0.09−0.63 % respectively. A ternary image of the radio-elements showed that the northern parts of Dekina and Soko have low concentrations of the three radionuclides whereas high concentration of the three radionuclides were noted at the northern part of Angba, southern part of Dekina and the western part of Ankpa. The ternary map also showed that Potassium in comparison to Uranium and Thorium in the study area is more abundant around the Angba area. The result of the radioelement maps and radiometric ratio maps revealed the presence of shale, feldspar, gabbro, granite and amphibolite in the study area. These mineral deposits could serve as raw materials for industries in Nigeria if harnessed.

A metagenomic analysis for combination therapy of multiple classes of antibiotics on the prevention of the spread of antibiotic-resistant genes

ABSTRACT Antibiotics used systemically to treat infections may have off-target effects on the gut microbiome, potentially resulting in the emergence of drug-resistant bacteria or selection of pathogenic species. These organisms may present a risk to the host and spread to the environment with a risk of transmission in the community. To investigate the risk of emergent antibiotic resistance in the gut microbiome following systemic treatment with antibiotics, this metagenomic analysis project used next-generation sequencing, a custom-built metagenomics pipeline, and differential abundance analysis to study the effect of antibiotics (ampicillin, ciprofloxacin, and fosfomycin) in monotherapy and different combinations at high and low doses, to determine the effect on resistome and taxonomic composition in the gut of Balb/c mice. The results showed that low-dose monotherapy treatments showed little change in microbiome composition but did show an increase in expression of many antibiotic-resistant genes (ARGs) posttreatment. Dual combination treatments allowed the emergence of some conditionally pathogenic bacteria and some increase in the abundance of ARGs despite a general decrease in microbiota diversity. Triple combination treatment was the most successful in inhibiting emergence of relevant opportunistic pathogens and completely suppressed all ARGs after 72 h of treatment. The relative abundances of mobile genetic elements that can enhance transmission of antibiotic resistance either decreased or remained the same for combination therapy while increasing for low-dose monotherapy. Combination therapy prevented the emergence of ARGs and decreased bacterial diversity, while low-dose monotherapy treatment increased ARGs and did not greatly change bacterial diversity.

Metagenome meta-analysis reveals an increase in the abundance of some multidrug efflux pumps and mobile genetic elements in chemically polluted environments.

Many human activities contaminate terrestrial and aquatic environments with numerous chemical pollutants that not only directly alter the environment but also affect microbial communities in ways that are potentially concerning to human health, such as selecting for the spread of antibiotic-resistance genes (ARGs) through horizontal gene transfer. In the present study, metagenomes available in the public domain from polluted (with antibiotics, with petroleum, with metal mining, or with coal-mining effluents) and unpolluted terrestrial and aquatic environments were compared to examine whether pollution has influenced the abundance and composition of ARGs and mobile elements, with specific focus on IS26 and class 1 integrons (intI1). When aggregated together, polluted environments had a greater relative abundance of ARGs than unpolluted environments and a greater relative abundance of IS26 and intI1. In general, chemical pollution, notably with petroleum, was associated with an increase in the prevalence of ARGs linked to multidrug efflux pumps. Included in the suite of efflux pumps were mexK, mexB, mexF, and mexW that are polyspecific and whose substrate ranges include multiple classes of critically important antibiotics. Also, in some instances, β-lactam resistance (TEM181 and OXA-541) genes increased, and genes associated with rifampicin resistance (RNA polymerases subunits rpoB and rpoB2) decreased in relative abundance. This meta-analysis suggests that different types of chemical pollution can enrich populations that carry efflux pump systems associated with resistance to multiple classes of medically critical antibiotics. IMPORTANCE The United Nations has identified chemical pollution as being one of the three greatest threats to environmental health, through which the evolution of antimicrobial resistance, a seminally important public health challenge, may be favored. While this is a very plausible outcome of continued chemical pollution, there is little evidence or research evaluating this risk. The objective of the present study was to examine existing metagenomes from chemically polluted environments and evaluate whether there is evidence that pollution increases the relative abundance of genes and mobile genetic elements that are associated with antibiotic resistance. The key finding is that for some types of pollution, particularly in environments exposed to petroleum, efflux pumps are enriched, and these efflux pumps can confer resistance to multiple classes of medically important antibiotics that are typically associated with Pseudomonas spp. or other Gram-negative bacteria. This finding makes clear the need for more investigation on the impact of chemical pollution on the environmental reservoir of ARGs and their association with mobile genetic elements that can contribute to horizontal gene transfer events.

Environmental selection and evolutionary process jointly shape genomic and functional profiles of mangrove rhizosphere microbiomes

AbstractMangrove reforestation with introduced species has been an important strategy to restore mangrove ecosystem functioning. However, how such activities affect microbially driven methane (CH4), nitrogen (N), and sulfur (S) cycling of rhizosphere microbiomes remains unclear. To understand the effect of environmental selection and the evolutionary process on microbially driven biogeochemical cycles in native and introduced mangrove rhizospheres, we analyzed key genomic and functional profiles of rhizosphere microbiomes from native and introduced mangrove species by metagenome sequencing technologies. Compared with the native mangrove (Kandelia obovata, KO), the introduced mangrove (Sonneratia apetala, SA) rhizosphere microbiome had significantly (p < 0.05) higher average genome size (AGS) (5.8 vs. 5.5 Mb), average 16S ribosomal RNA gene copy number (3.5 vs. 3.1), relative abundances of mobile genetic elements, and functional diversity in terms of the Shannon index (7.88 vs. 7.84) but lower functional potentials involved in CH4 cycling (e.g., mcrABCDG and pmoABC), N2 fixation (nifHDK), and inorganic S cycling (dsrAB, dsrC, dsrMKJOP, soxB, sqr, and fccAB). Similar results were also observed from the recovered Proteobacterial metagenome‐assembled genomes with a higher AGS and distinct functions in the introduced mangrove rhizosphere. Additionally, salinity and ammonium were identified as the main environmental drivers of functional profiles of mangrove rhizosphere microbiomes through deterministic processes. This study advances our understanding of microbially mediated biogeochemical cycling of CH4, N, and S in the mangrove rhizosphere and provides novel insights into the influence of environmental selection and evolutionary processes on ecosystem functions, which has important implications for future mangrove reforestation.

Pathogens and antibiotic resistance genes during the landfill leachate treatment process: Occurrence, fate, and impact on groundwater

Landfill leachate is an essential source of pathogens and antibiotic resistance genes (ARGs) in the environment. However, information on the removal behavior of pathogens and ARGs during the leachate treatment and the impact on surrounding groundwater is limited. In this study, we investigated the effects of leachate treatment on the removal of pathogens and ARGs with metagenomic sequencing, as well as the impact of landfill effluent on groundwater. It is shown that the leachate treatment could not completely remove pathogens and ARGs. Twenty-nine additional pathogens and twenty-nine ARGs were newly identified in the landfill effluent. The relative abundance of pathogens and multiple antibiotic resistance genes decreased after ultrafiltration but relative abundance increased after reverse osmosis. In addition, the relative abundances of Acinetobacter baumannii, Erwinia amylovora, Escherichia coli, Fusarium graminearum, Klebsiella pneumoniae, and Magnaporthe oryzae, as well as mdtH, VanZ, and blaOXA-53 increased significantly in the landfill effluent compared to the untreated leachate. The relative abundance of some mobile genetic elements (tniA, tniB, tnpA, istA, IS91) in leachate also increased after ultrafiltration and reverse osmosis. The size of pathogens, the size and properties of ARGs and mobile genetic elements, and the materials of ultrafiltration and reverse osmosis membranes may affect the removal effect of pathogens, ARGs and mobile genetic elements in leachate treatment process. Interestingly, the pathogens and ARGs in landfill effluent were transferred to groundwater according to SourceTracker. The ARGs, mobile genetic elements, and pathogens that are difficult to remove in the leachate treatment process, provide a reference for optimizing the leachate treatment process and improving the control of pathogens and ARGs. Furthermore, this study clarifies the effect of landfill leachate sources of pathogens and ARGs in groundwater.

A Chondritic Solar Neighborhood

A persistent question in exoplanet demographics is whether exoplanetary systems form from similar compositional building blocks to our own. Polluted white dwarf stars offer a unique way to address this question, as they provide measurements of the bulk compositions of exoplanetary material. We present a statistical analysis of the rocks polluting oxygen-bearing white dwarfs and compare their compositions to rocks in the solar system. We find that the majority of the extrasolar rocks are consistent with the composition of typical chondrites. Measurement uncertainties prevent distinguishing between chondrites and bulk Earth but do permit detecting the differences between chondritic compositions and basaltic or continental crust. We find no evidence of crust among the polluted white dwarfs. We show that the chondritic nature of extrasolar rocks is also supported by the compositions of local stars. While galactic chemical evolution results in variations in the relative abundances of rock-forming elements spatially and temporally on galaxy-wide scales, the current sample of polluted white dwarfs are sufficiently young and close to Earth that they are not affected by this process. We conclude that exotic compositions are not required to explain the majority of observed rock types around polluted white dwarfs and that variations between exoplanetary compositions in the stellar neighborhood are generally not due to significant differences in the initial composition of protoplanetary disks. Nonetheless, there is evidence from stellar observations that planets formed in the first several billion years in the Galaxy have lower metal core fractions compared with Earth on average.

Decoding the compositions of four bright r-process-enhanced stars

ABSTRACT There has been a concerted effort in recent years to identify the astrophysical sites of the r-process that can operate early in the galaxy. The discovery of many r-process-enhanced (RPE) stars (especially by the R-process Alliance collaboration) has significantly accelerated this effort. However, only limited data exist on the detailed elemental abundances covering the primary neutron-capture peaks. Subtle differences in the structure of the r-process pattern, such as the relative abundances of elements in the third peak, in particular, are expected to constrain the r-process sites further. Here, we present a detailed elemental-abundance analysis of four bright RPE stars selected from the HESP–GOMPA survey. Observations were carried out with the 10-m class telescope Gran Telescopio Canarias (GTC), Spain. The high spectral signal-to-noise ratios obtained allow us to derive abundances for 20 neutron-capture elements, including the third r-process peak element osmium (Os). We detect thorium (Th) in two stars, which we use to estimate their ages. We discuss the metallicity evolution of Mg, Sr, Ba, Eu, Os, and Th in r-II and r-I stars, based on a compilation of RPE stars from the literature. The strontium (Sr) abundance trend with respect to europium (Eu) suggests the need for an additional production site for Sr (similar to several earlier studies); this requirement could be milder for yttrium (Y) and zirconium (Zr). We also show that there could be some time delay between r-II and r-I star formation, based on the Mg/Th abundance ratios.

Composition constraints of the TRAPPIST-1 planets from their formation

ABSTRACT We study the formation of the TRAPPIST-1 (T1) planets starting shortly after Moon-sized bodies form just exterior to the ice line. Our model includes mass growth from pebble accretion and mergers, fragmentation, type-I migration, and eccentricity and inclination dampening from gas drag. We follow the composition evolution of the planets fed by a dust condensation code that tracks how various dust species condense out of the disc as it cools. We use the final planet compositions to calculate the resulting radii of the planets using a new planet interior structure code and explore various interior structure models. Our model reproduces the broader architecture of the T1 system and constrains the initial water mass fraction of the early embryos and the final relative abundances of the major refractory elements. We find that the inner two planets likely experienced giant impacts and fragments from collisions between planetary embryos often seed the small planets that subsequently grow through pebble accretion. Using our composition constraints, we find solutions for a two-layer model, a planet comprised of only a core and mantle, that match observed bulk densities for the two inner planets b and c. This, along with the high number of giant impacts the inner planets experienced, is consistent with recent observations that these planets are likely desiccated. However, two-layer models seem unlikely for most of the remaining outer planets, which suggests that these planets have a primordial hydrosphere. Our composition constraints also indicate that no planets are consistent with a core-free interior structure.

Distinct patterns of the soil and phyllosphere antibiotic resistome in natural forest ecosystems under an altitudinal gradient

Warming affects microbial functioning of soil and the phyllosphere across global ecosystems. However, little is known about the impact of increasing temperature on antibiotic resistome profiles in natural forests. To address this issue, we investigated antibiotic resistance genes (ARGs) in both soil and the plant phyllosphere using an experimental platform established in a forest ecosystem that delivers a temperature difference of 2.1 °C along an altitudinal gradient. Principal Coordinate Analysis (PCoA) showed that there were significant differences in the composition of soil and plant phyllosphere ARGs at different altitudes (P = 0.001). The relative abundance of phyllosphere ARGs and mobile genetic elements (MGEs) and soil MGEs increased with temperature. More resistance gene classes increased in abundance in the phyllosphere (10 classes) than soil (2 classes), and a Random Forest model analysis suggested that phyllosphere ARGs were more sensitive to temperature change than soil. Increasing temperature as a direct consequence of an altitudinal gradient, and the relative abundance of MGEs were the main drivers that shaped the profiles of ARGs in the phyllosphere and soil. Biotic and abiotic factors affected phyllosphere ARGs indirectly via MGEs. This study enhances our understanding of the influence of altitude gradients on resistance genes in natural environments.

The Distribution of Metallicities in the Local Galactic Interstellar Medium* *Based in part on observations made with the NASA/ESA Hubble Space Telescope, obtained from the MAST data archive at the Space Telescope Science Institute. The STScI is operated by the Association of Universities for Research in Astronomy, Inc., under NASA contract NAS5-26555. Based in part on data obtained from the

In this investigation, we present an analysis of the metallicity distribution that pertains to neutral gas in the local Galactic interstellar medium (ISM). We derive relative ISM metallicities for a sample of 84 sight lines probing diffuse atomic and molecular gas within 4 kpc of the Sun. Our analysis is based in large part on column density measurements reported in the literature for 22 different elements that are commonly studied in interstellar clouds. We supplement the literature data with new column density determinations for certain key elements and several individual sight lines important to our analysis. Our methodology involves comparing the relative gas-phase abundances of many different elements for a given sight line to simultaneously determine the strength of dust depletion in that direction and the overall metallicity offset. We find that many sight lines probe multiple distinct gas regions with different depletion properties, which complicates the metallicity analysis. Nevertheless, our results provide clear evidence that the dispersion in the metallicities of neutral interstellar clouds in the solar neighborhood is small (∼0.10 dex) and only slightly larger than the typical measurement uncertainties. We find no evidence for the existence of very low metallicity gas (as has recently been reported by De Cia et al.) along any of the 84 sight lines in our sample. Our results are consistent with a local Galactic ISM that is well mixed and chemically homogeneous.

New Chondritic Bodies Identified in Eight Oxygen-bearing White Dwarfs

We present observations and analyses of eight white dwarf stars (WDs) that have accreted rocky material from their surrounding planetary systems. The spectra of these helium-atmosphere WDs contain detectable optical lines of all four major rock-forming elements (O, Mg, Si, and Fe). This work increases the sample of oxygen-bearing WDs with parent body composition analyses by roughly 33%. To first order, the parent bodies that have been accreted by the eight WDs are similar to those of chondritic meteorites in relative elemental abundances and oxidation states. Seventy-five percent of the WDs in this study have observed oxygen excesses implying volatiles in the parent bodies with abundances similar to those of chondritic meteorites. Three WDs have oxidation states that imply more reduced material than found in CI chondrites, indicating the possible detection of Mercury-like parent bodies, but are less constrained. These results contribute to the recurring conclusion that extrasolar rocky bodies closely resemble those in our solar system, and do not, as a whole, yield unusual or unique compositions.

Quantitative correlation of refractory elemental abundances between rocky exoplanets and their host stars

Context. Stellar elemental abundances are generally used to constrain the interiors of rocky exoplanets by assuming planet’s relative abundances of major refractory elements (Fe, Mg, and Si) are similar to those of their host stars. Very recently, a non-one-to-one correlation was found among the compositions of low-mass planets and their host stars. It is therefore of great interest to further explore this correlation for larger samples of rocky exoplanets. Aims. We focus on a large sample of rocky exoplanets and compute their bulk elemental abundance ratios. We analyze the quantitative correlation between rocky exoplanets and their host stars by comparing the abundance ratios of these refractory elements. Methods. The interior of rocky exoplanets is assumed to be an iron-rich core overlaid with a silicate mantle. We constrained the bulk composition of rocky exoplanets from their measured mass and radius, using Bayesian statistical approaches. Then we used orthogonal distance regression (ODR) to characterize the compositional correlation between rocky exoplanets and their host stars. Results. Some rocky exoplanets are shown to have high iron-mass fractions and are thus likely to be iron-enriched super-Mercuries. We find the iron content of rocky exoplanets is dependent on the metallicity [Fe/H] of their host stars. The planets formed around a higher metallicity star generally span a wider range of iron masses, allowing for a higher iron content. Moreover, we directly compared the iron-mass fractions of rocky exoplanets with those deduced from the refractory elemental abundance ratios of their host stars. The results suggest that most rocky planets are more iron-enriched with respect to the initial protoplanetary disk.

Strong variation in sedimental antibiotic resistomes among urban rivers, estuaries and coastal oceans: Evidence from a river-connected coastal water ecosystem in northern China

Sediment is thought to be a vital reservoir to spread antibiotic resistance genes (ARGs) among various natural environments. However, the spatial distribution patterns of the sedimental antibiotic resistomes around the Bohai Bay region, a river-connected coastal water ecosystem, are still poorly understood. The present study conducted a comprehensive investigation of ARGs among urban rivers (UR), estuaries (ES) and Bohai Bay (BHB) by metagenomic sequencing. Overall, a total of 169 unique ARGs conferring resistance to 15 antimicrobial classes were detected across all sediment samples. The Kruskal-Wallis test showed that the diversity and abundance of ARGs in the UR were all significantly higher than those in the ES and BHB (p < 0.05 and p < 0.01), revealing the distance dilution of the sedimental resistomes from the river to the ocean. Multidrug resistance genes contained most of the ARG subtypes, whereas rifamycin resistance genes were the most abundant ARGs in this region. Our study demonstrated that most antimicrobial resistomes were highly accumulated in urban river sediments, whereas beta-lactamase resistance genes (mainly PNGM-1) dramatically increased away from the estuary to the open ocean. The relative abundance of mobile genetic elements (MGEs) also gradually decreased from rivers to the coastal ocean, whereas the difference in pathogenic bacteria was not significant in the three classifications. Among MGEs, plasmids were recognized as the most important carriers to support the horizontal gene transfer of ARGs within and between species. According to co-occurrence networks, pathogenic Proteobacteria, Actinobacteria, and Bacteroidetes were recognized as potential and important hosts of ARGs. Heavy metals, pH and moisture content were all recognized as the vital environmental factors influencing the distribution of ARGs in sediment samples. Overall, the present study may help to understand the distribution patterns of ARGs at a watershed scale, and help to make effective policies to control the emergence, spread and evolution of different ARG subtypes in different habitats.

Macrophage deficiency in CSF1R-knockout rat embryos does not compromise placental or embryo development.

Macrophages are an abundant cell population in the placenta and developing embryo and appear to be involved in processes of vascularization, morphogenesis, organogenesis, and hematopoiesis. The proliferation, differentiation, and survival are dependent on signals from the macrophage colony-stimulating factor receptor, CSF1R. Aside from the role in macrophages, Csf1r mRNA is highly expressed in placental trophoblasts. To explore the function of macrophages and Csf1r in placental and embryonic development, we analyzed the impact of homozygous Csf1r null mutation (Csf1rko) in the rat. In late gestation, IBA1+ macrophages were abundant in control embryos in all tissues, including the placenta, and greatly reduced in the Csf1rko. CSF1R was also detected in stellate macrophage-like cells and in neurons using anti-CSF1R antibody but was undetectable in trophoblasts. However, the neuronal signal was not abolished in the Csf1rko. CD163 was most abundant in cells forming the center of erythroblastic islands in the liver and was also CSF1R dependent. Despite the substantial reduction in macrophage numbers, we detected no effect of the Csf1rko on development of the placenta or any organs, the relative abundance of vascular elements (CD31 staining), or cell proliferation (Ki67 staining). The loss of CD163+ erythroblastic island macrophages in the liver was not associated with anemia or any reduction in the proliferative activity in the liver, but there was a premature expansion of CD206+ cells, presumptive precursors of liver sinusoidal endothelial cells. We suggest that many functions of macrophages in development of the placenta and embryo can be provided by other cell types in their absence.

Mitigation effects and microbial mechanism of two ecological earthworms on the uptake of chlortetracycline and antibiotic resistance genes in lettuce

The contamination of greenhouse vegetable soils with antibiotics and antibiotic resistance genes (ARGs), caused by the application of livestock and poultry manure, is a prominent environmental problem. In this study, the effects of two ecological earthworms (endogeic Metaphire guillelmi and epigeic Eisenia fetida) on the accumulation and transfer of chlortetracycline (CTC) and ARGs in a soil–lettuce system were studied via pot experiments. The results revealed that earthworm application accelerated the removal of the CTC from the soil and lettuce roots and leaves, with the CTC content reducing by 11.7–22.8 %, 15.7–36.1 %, and 8.93–19.6 % compared with that of the control, respectively. Both earthworms significantly reduced the CTC uptake by lettuce roots from the soil (P < 0.05) but did not change the CTC transfer efficiency from the roots to leaves. The high-throughput quantitative PCR results showed that the relative abundance of ARGs in the soil and lettuce roots and leaves decreased by 22.4–27.0 %, 25.1–44.1 %, and 24.4–25.4 %, respectively, with the application of earthworms. Earthworm addition decreased the interspecific bacterial interactions and the relative abundance of mobile genetic elements (MGEs), which helped reduce the dissemination of ARGs. Furthermore, some indigenous soil antibiotic degraders (Pseudomonas, Flavobacterium, Sphingobium, and Microbacterium) were stimulated by the earthworms. The results of redundancy analysis indicated that the bacterial community composition, CTC residues, and MGEs were the main parameters affecting the distribution of ARGs, accounting for 91.1 % of the total distribution. In addition, the bacterial function prediction results showed that the addition of earthworms reduced the abundance of some pathogenic bacteria in the system. Overall, our findings imply that earthworm application can substantially reduce the accumulation and transmission risk of antibiotics and ARGs in soil–lettuce systems, providing a cost-effective soil bioremediation practice for addressing antibiotic and ARGs contamination to guarantee the safety of vegetables and human health.