Relative Activity Concentration Research Articles

Comparison of Otsu and an adapted Chan–Vese method to determine thyroid active volume using Monte Carlo generated SPECT images

BackgroundThe Otsu method and the Chan–Vese model are two methods proven to perform well in determining volumes of different organs and specific tissue fractions. This study aimed to compare the performance of the two methods regarding segmentation of active thyroid gland volumes, reflecting different clinical settings by varying the parameters: gland size, gland activity concentration, background activity concentration and gland activity concentration heterogeneity.MethodsA computed tomography was performed on three playdough thyroid phantoms with volumes 20, 35 and 50 ml. The image data were separated into playdough and water based on Hounsfield values. Sixty single photon emission computed tomography (SPECT) projections were simulated by Monte Carlo method with isotope Technetium-99 m (^{text {99m}}Tc). Linear combinations of SPECT images were made, generating 12 different combinations of volume and background: each with both homogeneous thyroid activity concentration and three hotspots of different relative activity concentrations (48 SPECT images in total). The relative background levels chosen were 5 %, 10 %, 15 % and 20 % of the phantom activity concentration and the hotspot activities were 100 % (homogeneous case) 150 %, 200 % and 250 %. Poisson noise, (coefficient of variation of 0.8 at a 20 % background level, scattering excluded), was added before reconstruction was done with the Monte Carlo-based SPECT reconstruction algorithm Sahlgrenska Academy reconstruction code (SARec). Two different segmentation algorithms were applied: Otsu’s threshold selection method and an adaptation of the Chan–Vese model for active contours without edges; the results were evaluated concerning relative volume, mean absolute error and standard deviation per thyroid volume, as well as dice similarity coefficient.ResultsBoth methods segment the images well and deviate similarly from the true volumes. They seem to slightly overestimate small volumes and underestimate large ones. Different background levels affect the two methods similarly as well. However, the Chan–Vese model deviates less and paired t-testing showed significant difference between distributions of dice similarity coefficients (p-value <0.01).ConclusionsThe investigations indicate that the Chan–Vese model performs better and is slightly more robust, while being more challenging to implement and use clinically. There is a trade-off between performance and user-friendliness.

Monte Carlo-derived 99m Tc uptake quantification with commercial planar MBI: Tumor and breast activity concentrations.

Current molecular breast imaging (MBI) images are limited to qualitative evaluation, not absolute measurement, of 99m Tc uptake in benign and malignant breast tissues. This work assesses the accuracy of previously-published and newly-proposed tumor and normal breast tissue 99m Tc uptake MBI measurements using simulations of a commercial dual-headed planar MBI system under typical clinical and acquisition protocols. Quantification techniques were tested in over 4000 simulated acquisitions of spherical and ellipsoid tumors with clinically relevant uptake conditions using a validated Monte Carlo application of the GE Discovery NM750b system. The evaluated techniques consisted of four tumor total activity methodologies (two single-detector-based and two geometric-mean-based), two tumor MBI volume methodologies (diameter-based and ROI-based), and two normal tissue activity concentration methodologies (single-detector-based and geometric-mean-based). The most accurate of these techniques were then used to estimate tumor activity concentrations and tumor to normal tissue relative activity concentrations (RC). Single-detector techniques for tumor total activity quantification achieved mean (standard deviation) relative errors of 0.2% (4.3%) and 1.6% (4.4%) when using the near and far detector images, respectively and were more accurate and precise than the measured 8.1% (5.8%) errors of a previously published geometric-mean technique. Using these activity estimates and the true tumor volumes resulted in tumor activity concentration and RC errors within 10% of simulated values. The precision of tumor activity concentration and RC when using only MBI measurements were largely driven by the errors in estimating tumor MBI volume using planar images (±30% inter-quartile range). Planar MBI images were shown to accurately and reliably be used to estimate tumor total activities and normal tissue activity concentrations in this simulation study. However, volumetric tumor uptake measurements (i.e., absolute and relative concentrations) are limited by inaccuracies in MBI volume estimation using two-dimensional images, highlighting the need for either tomographic MBI acquisitions or anatomical volume estimates for accurate three-dimensional tumor uptake estimates.

Introduction to the special issue on the Life in Antarctica: Boundaries and Gradients in a Changing Environment (XIth SCAR Biology Symposium)

Scientific research in Antarctica has reached maturity in recent decades. The interest in issues related to knowledge about the southern polar regions has increased significantly among researchers from all scientific and technological disciplines. Among the various fields, biology comprises perhaps the highest concentration of related activities and encompasses the most diverse research topics. This increase in the research activity has to be translated in a greater coordination research efforts. The Scientific Committee of Antarctic Research has positioned itself as the focal point of this activity, with the organized symposiums being the best forum to share and report progress in various fields. This was the philosophy of XIth Scientific Committee on Antarctic Research Symposium held in Barcelona in July 2013. The different contributions of the symposium developed around a main topic: “Life in Antarctica: Boundaries and Gradients in a Changing Environment”. The symposium had the objective of linking the functional importance of land and water ecosystems with their bio-complexity under an ecosystem perspective. Such an approach will lead to a better understanding of Antarctic food webs, effects of human impacts (e.g., ozone hole, climate change, tourism), flexible boundaries and dynamic gradients in Antarctic ecosystems, as well as Antarctic marine biodiversity through its patterns, processes and trends.

Progress in human embryonic stem cell research in the United States between 2001 and 2010.

On August 9th, 2001, the federal government of the United States announced a policy restricting federal funds available for research on human embryonic stem cell (hESCs) out of concern for the “vast ethical mine fields” associated with the creation of embryos for research purposes. Until the policy was repealed on March 9th, 2009, no U.S. federal funds were available for research on hESCs extracted after August 9, 2001, and only limited federal funds were available for research on a subset of hESC lines that had previously been extracted. This paper analyzes how the 2001 U.S. federal funding restrictions influenced the quantity and geography of peer-reviewed journal publications on hESC. The primary finding is that the 2001 policy did not have a significant aggregate effect on hESC research in the U.S. After a brief lag in early 2000s, U.S. hESC research maintained pace with other areas of stem cell and genetic research. The policy had several other consequences. First, it was tied to increased hESC research funding within the U.S. at the state level, leading to concentration of related activities in a relatively small number of states. Second, it stimulated increased collaborative research between US-based scientists and those in countries with flexible policies toward hESC research (including Canada, the U.K., Israel, China, Spain, and South Korea). Third, it encouraged independent hESC research in countries without restrictions.

Depth Profile of 137Cs Fallout in Soil in Cameron Highlands

137Cs is one of the important man-made radionuclide introduced into the environment through nuclear weapons testing and nuclear accidents. In Cameron Highlands meaningful activity concentration of 137Cs radionuclide has been measured. The present study embarked on the determination of 137Cs activity concentration in soils of the sloping area. This would enable the distribution profile in the soil be investigated. Soil profile samples were collected by using scrapper plate in the sloping area which covers top, centre and bottom of the hills and a core in the forest for comparison purpose. The scrapper is 40 cm long and able to obtain slice of 2 cm interval of soil with diameter of 15.2 cm. Soil samples were oven dried, ground, sieved, homogenize and seal properly into a plastic container. The 137Cs activity concentration was measured by using low background coaxial hyper pure germanium detector gamma spectrometer based on 137Cs 661.66 KeV gamma ray. Results showed there were trend of low activity on the top of the hill and subsequently increases downhill. In all location, two trends of profiles were observed starting from the depth of 0 cm to 18 cm and 18 cm to 30 cm which indicating the presence of two sources of 137Cs. The 137Cs activity concentrations range detected at top location was from 0.05 Bq/kg to 1.53 Bq/kg, centre was 0.22 Bq/kg to 2.11 Bq/kg, bottom was from 0.00 Bq/kg to 2.03 Bg/kg and forest was 0.00 Bq/kg to 0.96 Bq/kg. The basic parameters of the soil such as pH (top; 3.72, centre; 4.26, bottom; 4.02), density (top; 1.45 g/m3, centre; 1.41 g/m3 , bottom; 1.59 g/m3 ) and organic matter content (top; 17.08 %, centre; 16.75 %, bottom; 20.32 %) also being tested. These results show that the relative activity concentration of 137Cs at the top is lowest, while at the bottom is highest, representing the presence of downward transport. However, identical pattern of profiles were observed at the three points of the slopes.

A new approach to analysis of relationships between 137Cs activity concentrations in forest soil horizons.

The measurements results of 137Cs activity concentrations in forest soil profiles are discussed. In studies some simplifications were considered. First of them concerns disregarding of soil subtype in data analysis. However initially this parameter was considered in data analysis, it was finally ignored. The second assumption drops information about specific soil horizon. Description of 137Cs accumulation is based on relationships between its relative activity concentrations in soil layers. The model formulation was based on the results of exploratory data analysis of the relative 137Cs activity concentrations. In studies the methods designed for compositional data analysis were used. The results of analysis showed that the relationships between the relative activity concentrations of 137Cs in soil layers, due to their nature, might be divided into two groups. The first of them concerns layers located close to soil surface. The relative activity concentrations of 137Cs in these layers are proportional to each other, and distribution mechanism of cesium within them has the characteristics of the process leading to thermodynamic equilibrium. The second group is related to layers that are located deeper. The calculation results suggest lack of thermodynamic equilibrium between these layers and layers situated above. Utilization of a linear model for description of changes in relative activity concentrations of 137Cs in deeper layers supposes that these changes occur much slower than in layers lying above.

A Voxel-Dose Algorithm of Heterogeneous Activity Distribution for Monte–Carlo Simulation of Radionuclide Therapy Dosimetry

The organ or tumor activity is not uniform due to inhomogeneous expression/distributions of receptors/antigens and the nonuniform vascularization of the tumor tissue. However, most patient-specific three-dimensional Monte-Carlo methods for radionuclide dosimetry have dealt with quasi-homogeneous activity distributions. A voxel-by-voxel activity sample algorithm (VM) without artifacts is presented to calculate the dose of the heterogeneous activity distribution for radionuclide dosimetry. The source particle location is sampled according to the activity spatial distribution. The source particle weight is imparted by the relative activity concentration of its origination voxel. This algorithm is applied to calculate the dose volume histogram for multiple independent activity regions with Gauss diffusion activity distributions and then compared with the level partition method (LM). The minimal response and the mean tolerant initial total activity threshold required by tumor control probability and normal tissue complication probability for radioimmunotherapy ((131)I-RIT) also were evaluated by the voxel-by-voxel sample algorithm and the LM. The effective clearance half-time is assumed to be equal to its physical half-life (i.e., 8.02 days for (131)I). The result shows that the new algorithm is more consistent with the weighted superposition of the quasi-homogeneous activity distribution than the LM, especially for the multiple independent activity regions composed of different amounts of voxels. The new algorithm effectively avoids the leveling/binning artifacts to the heterogeneous activity distribution. The (131)I-RIT simulation also showed that the minimal response initial total activity threshold of tumors will be much more than the mean tolerant initial total activity threshold of normal organs (e.g., kidney) with the activity heterogeneous grade deteriorating. A VM is presented to simulate the dose of the heterogeneous activity distribution for radionuclide dosimetry. The new algorithm effectively avoids the leveling/binning artifacts to the heterogeneous activity distribution.

Comparison of two immunochemical assays for measuring thrombin-activatable fibrinolysis inhibitor concentration with a functional assay in patients with acute coronary syndrome

TAFI was measured as relative activity concentration (Pefakit) and antigen concentration (Haemochrom and Asserachrom) both acutely and during convalescence in patients suffering from acute coronary syndrome, and in a group of healthy controls. There was a rather weak but significant correlation between the activity and antigen concentrations. The results obtained by the Haemochrom method, assumed to measure the concentrations of all forms of TAFI, were lower than those by the Asserachrom method although the latter is assumed to only measure the pro-TAFI concentration. Both immunoassays gave lower results than the functional method (Pefakit). The patients in the convalescence phase showed a higher activity concentration than the healthy controls. The Asserachrom showed a higher concentration in the acute and convalescence phases of the patients compared to the controls, whereas the Haemochrom did not show any such difference. This could be related to different species of the TAFI molecule and thus different reactivity to the antibodies. Therefore the optimal choice of assay for determination of TAFI in different clinical studies is of importance. The Pefakit and Asserachrom seem to be appropriate candidates.

Radioactivity in soils and various foodstuffs from the Gediz River Basin of Turkey

The aim of this study is to determine the enhancement in natural radioactivity level of soils due to the usage of fertilizers, particularly phosphate fertilizers in agricultural lands of Gediz River basin, Western Turkey. In addition, the activity concentrations of naturally occurring radionuclides in some vegetables collected from the same lands were investigated in order to assess any contribution to the effective internal dose after ingestion. Radioactivity concentrations of natural radionuclides, namely Ra 226 , Th 232 and K 40 , in fertilized, non-fertilized soils and vegetables were measured by NaI(Tl) gamma spectrometer in order to assess the implications of the extended use of phosphate fertilizers in agriculture in recent years. Activity concentration of Po 210 , an alpha particle emitter, were also analysed using a ZnS(Ag) alpha counter after electrodeposition process. In addition, physical and chemical characteristics of soil samples were investigated and analysed for any correlation between the soil characteristics and their radionuclide contents. In vegetables collected from agricultural fields of the basin the ranges of the relative activity concentrations of K 40 , Ra 226 , Th 232 and Po 210 are found to be 491.62–2324.51, 15.96–52.80, ND–10.54 and 8.40 – 22.20 Bq kg - 1 , respectively. In soils of agricultural lands, where vegetables are collected, the activity concentrations of K 40 , Ra 226 , Th 232 and Po 210 are 325.89–530.52, 46.05–68.83, 9.29–50.57 and 36.75 – 56.70 Bq kg - 1 , respectively. For non-fertilized fields, activity concentrations of soils are found to be within 240.40 – 403.09 Bq kg - 1 for K 40 , 35.61 – 62.64 Bq kg - 1 for Ra 226 , 7.40 – 38.53 Bq kg - 1 for Th 232 and 32.50 – 41.14 Bq kg - 1 for Po 210 .

Short‐term distribution of 134Cs in relation to 51Cr‐EDTA after intravenous dose in goats

The aim of this study was to gather information about the short-term rate of caesium uptake (incorporation) in different animal tissues and explain them with known physiological mechanisms affecting ion distribution. Six goats were given an intravenous bolus containing (134)Cs as a tracer and (51)Cr-EDTA as an extracellular marker. After 30 min, the animals were killed and the activity concentration of radioactive isotopes in different tissues and fluid compartments were measured. The highest relative activity concentration of (134)Cs was found in kidney cortex, with a tissue/plasma-ratio around 50. In urine, the ratio varied between 5 and 28. In the salivary gland, cardiac muscle and small intestine the ratio was around 11, 7 and 6, respectively. The contents of small intestine had an average activity concentration five times that of plasma. In skeletal muscle the terminal activity concentration was surprisingly low, with a tissue/plasma ratio mostly far less than unity. Even in connective tissue and cartilage the terminal activity concentration was generally higher than in skeletal muscle. The rate of uptake of caesium varies widely from tissue to tissue. Many of these differences can be explained with differences in Na,K-ATPase activity. Also, perfusion and accessibility play a role in some tissues, like brain and possibly part of the skeletal muscles. The short-term distribution of caesium differs distinctly from the long-term distribution reported in literature.

Radiostrontium contamination of soil and vegetation within the Semipalatinsk test site

The Semipalatinsk nuclear test site (STS) in the Republic of Kazakhstan was an important site for testing atomic bombs and other civil and military nuclear devices of the former Soviet Union. Results are presented from investigations on the extent of radiostrontium contamination in soils and vegetation at the technical areas of the STS, where the tests were conducted and in pastures used by farmers for grazing animals or for hay production. Our data are compared with those reported largely in the recent Russian language literature that has been reviewed. The extent of (90)Sr contamination of soil is highly variable over the STS with the highest values associated with the technical areas, particularly the Degelen mountains. Recently measured values in both the present data and the Russian language literature confirm the relatively high current contamination of soil and vegetation in the vicinity of tunnels and associated watercourses in the Degelen area. The proportion of (90)Sr in soil which could not be extracted with 6 M HCl was only an average of 20%, which is low compared to other test site areas and possibly indicates a relatively high mobility in this area, because the (90)Sr is derived from leakage from explosion tunnels along watercourses rather than being associated with fused silicates. A comparison of relative activity concentrations in soil and vegetation suggests that the transfer of (90)Sr to vegetation on the STS is high compared to that of (137)Cs and plutonium.

Derivation of working levels for radionuclides in animal feedstuffs for use following a nuclear accident.

Maximum permitted levels of radiocesium in marketed animal feedstuffs have been specified by the European Commission for use in the event of a future nuclear accident. However, more specific guidance is required for use under UK conditions. Using information on typical animal diets, relative activity concentrations in feedstuffs and feed-to-product transfer, practical working levels have been derived for (134,137)Cs and 90Sr in animal feedstuffs and drinking water; application of these levels should ensure that activity concentrations in milk, meat, or eggs do not exceed the relevant Council Food Intervention Level. Despite the complexity of some animal diets, only one or two feedstuffs typically contribute 5% or more to intakes of activity. The working levels for radiocesium in these feedstuffs ranged from a factor of 20 higher than the maximum permitted levels to a factor of around 20 lower. In most cases, the maximum permitted levels are unnecessarily cautious. Working levels for 90Sr in feedstuffs are generally higher than those for radiocesium, except for feedstuffs for dairy cattle and laying hens. Factors affecting the practicability and effectiveness of changes in diet as a countermeasure to reduce activity concentrations in animal products were also considered within this study.

Correction for scatter in 3D brain PET using a dual energy window method

A method for scatter correction using dual energy window acquisition has been developed and implemented on data collected with a brain-PET tomograph operated in the septa retracted, 3D mode. Coincidence events are assigned to (i) an upper energy window where both photons deposit energy between 380 keV and 850 keV or (ii) a lower energy window where one or both photons deposit within 200 keV and 380 keV. Scaling parameters are derived from measurements of the ratios of counts from line sources due to scattered and unscattered events in the two energy windows in head-sized phantoms. A scaled subtraction of the two energy windows produces a distribution of scatter which is smoothed prior to subtraction from the upper energy window. In phantoms, the correction was found to restore the uniformity, contrast and linearity of activity concentration. Relative activity concentrations were restored to within 7% of their true values in a multicompartment phantom. The method was found to provide accurate correction for scattered events arising from activity outside the direct detector field of view. In a three-compartment phantom containing water, and scanned in dynamic, multiframe mode, the half-lives of the two isotopes were restored to within 2% of their true value.

An International Reference Material for Radiocarbon Dating

In August 1980, the National Bureau of Standards (NBS) issued, in the form of oxalic acid, a new International Reference Material of contemporary 14C for use in radiocarbon dating laboratories. This reference material was to replace the 1975 oxalic-acid standard, supplies of which had been practically exhausted in 1978. The preparation of the new oxalic-acid standard was described in a preliminary report, as were, also, the results then available for the activity-concentration ratio of the new to the old standard obtained by a number of leading international laboratories.With the recent completion of the analysis of all results submitted by the participating laboratories, NBS plans to issue these recently calibrated samples of oxalic acid as an NBS Standard Reference Material. There is, however, no significant difference in the reported value of its activity concentration, relative to that of the 1957 standard, from that given provisionally in 1980.Subsequent to our report (Cavallo and Mann, 1980) a further measurement of relative activity concentration was reported by the Radiocarbon Laboratory of Peking University. Their value, and that also for δ13C, is insignificantly different from the average value of the results submitted by the eleven laboratories that participated in the international comparison organized by NBS.