Role Of Melatonin In Regulation Research Articles

The regulatory role of melatonin in pituitary thyroid-stimulating hormone synthesis through casein kinase 1α

The regulatory role of melatonin in pituitary thyroid-stimulating hormone synthesis through casein kinase 1α

Exogenously applied melatonin enhanced chromium tolerance in pepper by up-regulating the photosynthetic apparatus and antioxidant machinery

Chromium (Cr) is widely recognized as a highly toxic metal pollutant that adversely affects the agricultural productivity and poses considerable risks to human health. The regulatory role of melatonin (MEL) in Cr stress is well recognized, however, the potential role of MEL in enhancing Cr stress tolerance in pepper is rarely investigated. This study examined the possible effect of MEL in alleviating the adverse effects of Cr toxicity in pepper seedlings. When pepper seedlings were exposed to Cr toxicity, they showed a significant reduction in growth traits, photosynthetic efficiency, root morphological characteristics, and biochemical attributes. Cr treatment greatly caused oxidative damage that was directly related to increased EL, H2O2, O2•−, and MDA production. Conversely, MEL supplementation significantly mitigated the phytotoxic effects of Cr on root morphology, gas exchange elements, and leaf photosynthetic capacity, and restored the growth of pepper seedlings. MEL application stimulated the plant's defense system by enhancing the modulation of antioxidant enzymes, AsA-GSH pools, maintaining the glyoxalase enzymes activity, and reducing oxidative damage. Notably, MEL supplementation significantly reduced Cr accumulation from root to shoot. Hence, MEL supplementation might be regarded as a successful approach for reducing Cr accumulation and its adverse effects in pepper seedlings cultivated in contaminated soils. The research may also provide novel perspectives on the potential application of MEL in mitigating heavy metal toxicity in pepper seedlings.

Physiological and Biochemical Mechanisms of Exogenous Melatonin Regulation of Saline–Alkali Tolerance in Oats

Saline–alkali stress is one of the major factors limiting oat seed germination. The regulatory role of melatonin (MT) as a naturally occurring active substance is well known, but the mechanism of MT-mediated intrinsic physiological regulation of oat seed germination under saline–alkali stress is unclear. Therefore, this study investigated (1) the variability of different MT seed soaking concentrations and times on the germination of oat seeds under saline–alkali stress, and (2) the possible physiological regulatory mechanisms of MT on the germination of oat seeds under saline–alkali stress. The results showed that seed vigor was significantly reduced under saline–alkali stress, and seed germination of oats was significantly inhibited; different concentrations of MT seed soaking treatments improved the germination rate, germination potential, germination index, vigor index, root length, germ length, fresh weight, and dry weight; and, overall, treatment improved seed germination and exhibited the phenomenon of “low promotion and high inhibition”. The 100 μmol·L−1 MT seed soaking treatment effectively enhanced the activities of seed antioxidant enzymes (SOD, POD, CAT, and APX) and nonenzymatic antioxidants (GSH and AsA), affected the AsA-GSH cycle, and effectively increased the contents of osmoregulatory substances (proline, protein, and soluble sugar) by reducing levels of H2O2, OH−, and MDA, thus enhancing the tolerance of oats to saline–alkali and promoting seed germination. In conclusion, MT has a positive effect on the saline–alkali tolerance of oat seeds.

Regulatory role of melatonin in Notch1 signaling pathway in cerebral cortex of Aβ1-42-induced Alzheimer's disease rat model.

Soluble Amyloid-beta (Aβ) oligomers are thought to play a key role in the pathogenesis of Alzheimer's disease (AD), which is the most common age-associated neurodegenerative diseases with obvious neuropathological changes and functional decline in both cortical and subcortical regions. Melatonin is ubiquitously distributed and multifunctioning indoleamine. Accumulating studies support that melatonin is potential therapeutic molecule for AD through modulating a broad variety of signaling pathways. In recent years, Notch1 signaling pathway is been known involved in dynamic changes in the cellular architecture and function of adult brain, as well as associated with the pathophysiology of AD and other neurodegenerative disorders. In this study, we performed real-time polymerase chain reaction, immunohistochemistry and western blotting analyses using the cerebral cortical tissues of Aβ1-42 oligomers-induced AD rats with or without melatonin treatment. Our results showed that soluble Aβ1-42 oligomers decreased the expression of the main components of Notch1 signaling pathway, Notch1, NICD and Hes1 in the cerebral cortex, and melatonin could restore the level of Notch1, NICD and Hes1. This observation suggests that targeting of Notch1 signaling might be a promising therapeutic approach for AD and other age-associated neurodegenerative diseases, and melatonin might serve as a potential therapeutic agent for AD and other age-associated neurodegenerative diseases.

Research progress on regulation of plant stress resistance by melatonin

As a signaling molecule, melatonin can regulate plant circadian rhythm, seed germination, root and flower development, and enhance plant resistance to various stresses. In this paper, the regulatory role of melatonin in plant abiotic stress and biological stress response was summarized, and the application prospect of melatonin in plant growth and development and stress resistance was prospected, in order to provide reference for improving plant stress resistance by using melatonin.

Melatonin regulates proliferation, apoptosis and invasion of trophoblasts in preeclampsia by inhibiting endoplasmic reticulum stress.

Clinical evidence indicated the activation of endoplasmic reticulum stress (ERS) in pregnant women with preeclampsia (PE), and the regulatory role of melatonin (MT) in ERS. This study aims to explore the possible effect and mechanism of MT on ERS and on the infiltration of trophoblasts in PE. The serum expression levels of MT and GRP78 in pregnant women with PE were measured. The cell proliferation, invasion, migration and apoptosis of trophoblasts were also determined. The trophoblast cell infiltration in placenta tissues was detected in EVOS image system. The expressions of ERS related proteins were measured by RT-qPCR and western blot. The PE-serum treatment on HTR-8/SVneo cells led to activated ERS and suppressed cell biological functions. PE mouse models after MT treatment or 4-PBA treatment had reduced blood pressure, proteinuria, apoptosis and increased foetus and placenta weight, in addition to enhanced cell infiltration. In vivo and in vitro evidence demonstrated MT can simultaneously suppress ERS and ASK1/JNK signal pathway in PE to promote the infiltration of trophoblasts.

Role of melatonin in regulation of lipid accumulation, autophagy and salinity-induced oxidative stress in microalga Monoraphidium sp. QLY-1

The aim of this study was to explore the effect of melatonin (MT) on lipid accumulation in Monoraphidium sp. QLY-1 under salinity stress. The interconnection between MT, oxidative stress and autophagy in the regulation of lipid synthesis was also clarified. MT application significantly promoted the lipid content, peaking at 51.74%, which was 1.16- and 1.44-fold higher than those induced by salinity stress alone (44.77%) and control group (35.96%), respectively. Exogenous MT also upregulated the transcription levels of lipogenesis-related genes and decreased salinity stress-induced oxidative stress. Interestingly, the application of MT upregulated autophagic activity, which led to the 1.47-fold upregulation of autophagy-related atg8 gene expression. Additional results demonstrated that autophagy promotion led to further increases in lipid accumulation by involving lipogenesis gene expression and ROS signalling in cells treated with MT under salinity stress conditions. The maximum lipid content (54.26%) was achieved by treatment with MT and rapamycin (RAPA), an activator of autophagy. Taken together, these findings indicate that combination of MT and salinity stress is a valuable strategy for enhancing lipid production in microalgae and provides novel insights into the regulatory mechanisms underlying the effects of MT and autophagy on the promotion of lipid synthesis and improvements to algal tolerance.

The regulatory role of melatonin in skeletal muscle.

Melatonin (N-acetyl-5-methoxy-tryptamine) is an effective antioxidant and free radical scavenger, that has important biological effects in multiple cell types and species. Melatonin research in muscle has recently gained attention, mainly focused on its role in cells or tissue repair and regeneration after injury, due to its powerful biological functions, including its antioxidant, anti-inflammation, anti-tumor and anti-cancer, circadian rhythm, and anti-apoptotic effects. However, the effect of melatonin in regulating muscle development has not been systematically summarized. In this review, we outline the latest research on the involvement of melatonin in the regulation of muscle development and regeneration in order to better understand its underlying molecular mechanisms and potential applications.

Psychological adaptation and peculiarities of melatonin synthesis in women with infertility of various genesis

To improve medical care for families suffering from infertility, a comprehensive approach is needed taking into account the psychoemotional state of a woman and the adaptive capabilities of her body.Aim. To determine the presence of psychological adaptation disorders and features of melatonin synthesis in women with infertility of various genesis.Materials and methods. 115 women with infertility (the main group) were under observation. The control group consisted of 90 fertile women who had born children. The presence of sleep disorders (the table by A. M. Veyn), stress level (the Holmes and Rahe Stress Scale), state and trait anxiety (the Ch. D. Spielberger Anxiety Scales, Yu. L. Khanin, 1976) and depression (the Beck Depression Scale) were assessed. The determination of melatonin serum level was performed by the method of enzyme immunoassay (ELISA).Results. Women with infertility are characterized by violations of psychological adaptation, manifested by sleep disorders (67.0 %), increased stress level (47.8 %), increased state anxiety (47.0 %) and depressive states (21.7 %). Significant disorders are observed in women with idiopathic and hormonal infertility, the least pronounced changes – in infertility caused by an immunological factor. Hypomelatoninemia is observed in 36.5 % of women with infertility and in 20.9 % – hypermelatoninemia. Such multidirectional changes indicate desynchronization of melatonin synthesis, and confirm the dysadaptative disorders in infertility. The highest incidence of melatonin secretion abnormalities was observed in hormonal infertility, and the lowest - in immunological infertility. The regulatory role of melatonin in psychological adaptation is confirmed by its level correlation with the corresponding indicators scores: sleep disorders (ρ = -0.51), stress level (ρ = -0.39), state (ρ = -0.37) and trait anxiety (ρ = -0.26), depression level (ρ = 0.22).Conclusions. A high frequency of psychological dysadaptosis in women with infertility, especially with hormonal and idiopathic infertility, and the regulatory role of melatonin have been revealed, which opens the prospects for psychocorrection methods and therapy with melatonin preparations inclusion in the treatment of infertility.

Regulatory role of melatonin and BMP-4 in prolactin production by rat pituitary lactotrope GH3 cells

The effects of melatonin on prolactin production and its regulatory mechanism remain uncertain. We investigated the regulatory role of melatonin in prolactin production using rat pituitary lactotrope GH3 cells by focusing on the bone morphogenetic protein (BMP) system. Melatonin receptor activation, induced by melatonin and its receptor agonist ramelteon, significantly suppressed basal and forskolin-induced prolactin secretion and prolactin mRNA expression in GH3 cells. The melatonin MT2 receptor was predominantly expressed in GH3 cells, and the inhibitory effects of melatonin on prolactin production were reversed by treatment with the receptor antagonist luzindole, suggesting functional involvement of MT2 action in the suppression of prolactin release. Melatonin receptor activation also suppressed BMP-4-induced prolactin expression by inhibiting phosphorylation of Smad and transcription of the BMP-target gene Id-1, while BMP-4 treatment upregulated MT2 expression. Melatonin receptor activation suppressed basal, BMP-4-induced and forskolin-induced cAMP synthesis; however, BtcAMP-induced prolactin mRNA expression was not affected by melatonin or ramelteon, suggesting that MT2 activation leads to inhibition of prolactin production through the suppression of Smad signaling and cAMP synthesis. Experiments using intracellular signal inhibitors revealed that the ERK pathway is, at least in part, involved in prolactin induction by GH3 cells. Thus, a new regulatory role of melatonin involving BMP-4 in prolactin secretion was uncovered in lactotrope GH3 cells.

Nocturnal Melatonin Profiles in Patients with Delayed Sleep-Wake Phase Disorder and Control Sleepers.

A significant delay in the timing of endogenous circadian rhythms has been associated with delayed sleep phase disorder (DSPD). More recently, other mechanisms have also been proposed to account for this disorder. To further explore the etiology of DSPD, the present study compared nocturnal melatonin profiles of 26 DSPD patients (18 males, 8 females; age, 21.73 ± 4.98 years) and 17 normally timed good sleepers (10 males, 7 females; age, 23.82 ± 5.23 years) in a time-free, dim-light (<10 lux) laboratory environment. A 30-h modified constant routine with alternating 20-min sleep opportunities and 40 min of enforced wakefulness was used to measure the endogenous melatonin circadian rhythm. Salivary melatonin was sampled half-hourly from 1820 h to 0020 h and then hourly from 0120 h to 1620 h. DSPD patients had significantly later timed melatonin profiles that were delayed by approximately 3 h compared to normal sleepers, and there were no notable differences in the relative duration of secretion between groups. However, melatonin secretion between dim-light melatonin onset (DLMO) and acrophase was less prominent in DSPD patients compared to good sleepers, who showed a more acute initial surge of melatonin following the DLMO. Although the regulatory role of melatonin is unknown, abnormal melatonin profiles have been linked to psychiatric and neurological disorders (e.g., major depression, obsessive compulsive disorder, Parkinson disease). These results therefore suggest that in addition to a delayed endogenous circadian rhythm, a diminished initial surge of melatonin secretion following DLMO may contribute to the etiology of DSPD.

Seasonal modulation of immunity by melatonin and gonadal steroids in a short day breeder goat Capra hircus

Role of melatonin in regulation of immunity and reproduction has never been studied in detail in goats. The aim of the present study was to explore hormonal regulation of immunity in goats with special reference to melatonin. Plasma of male and female goats (n = 18 per sex per season) was processed for hormonal (estrogen, testostrone, and melatonin) and cytokine (interleukin [IL-2], IL-6, and tumor necrosis factor α) measurements during three seasons, i.e., summer, monsoon, and winter. To assess cell-mediated immune response, percent stimulation ratio of thymocytes was recorded during three seasons. To support and establish the modulation by hormones, Western blot analysis for expressions of melatonin receptors (MT1, MT2), androgen receptor, and estrogen receptor α and estimations of marker enzymes, arylalkylamine N-acetyltransferase for melatonin and 3β-hydroxysteroid dehydrogenase activities for steroidogenesis were performed in thymus. All the hormones and cytokines were estimated by commercial kits. Biochemical, immunologic, and Western blot analyses were done by standardized protocols. We noted a significant increase in estrogen and testosterone levels (P < 0.05) in circulation during monsoon along with melatonin (P < 0.05) presenting a parallel relationship. Expressions of melatonin receptors (MT1 and MT2) in thymus of both the sexes were significantly high (P < 0.01) during winter. Estrogen receptor α expression in female thymus was significantly high during monsoon (P < 0.05). However, androgen receptor showed almost static expression pattern in male thymus during three seasons. Further, both arylalkylamineN-acetyltransferase and 3β-hydroxysteroid dehydrogenase enzyme activities were significantly high (P < 0.05; P < 0.01, respectively) during monsoon. These results suggest that there may be a functional parallelism between gonadal steroids and melatonin as melatonin is progonadotrophic in goats. Cell-mediated immune parameters (percent stimulation ratio of thymocytes) and circulatory levels of cytokines (IL-2, IL-6, and tumor necrosis factor α) were significantly high (P < 0.01) during monsoon. In vitro supplementation of gonadal steroids to T-cell culture suppressed immunity but cosupplementation with melatonin restored it. Further, we may also suggest that reproductive and immune seasonality are maintained by variations in circulatory hormones and local synthesis of melatonin and gonadal steroids. These functional interactions between melatonin and gonadal steroid might be of great importance in regulating the goat immunity by developing some hormonal microcircuit (gonadal steroid and melatonin) in lymphatic organs.

Arabidopsis transcriptome analysis reveals key roles of melatonin in plant defense systems.

Melatonin is a ubiquitous molecule and exists across kingdoms including plant species. Studies on melatonin in plants have mainly focused on its physiological influence on growth and development, and on its biosynthesis. Much less attention has been drawn to its affect on genome-wide gene expression. To comprehensively investigate the role(s) of melatonin at the genomics level, we utilized mRNA-seq technology to analyze Arabidopsis plants subjected to a 16-hour 100 pM (low) and 1 mM (high) melatonin treatment. The expression profiles were analyzed to identify differentially expressed genes. 100 pM melatonin treatment significantly affected the expression of only 81 genes with 51 down-regulated and 30 up-regulated. However, 1 mM melatonin significantly altered 1308 genes with 566 up-regulated and 742 down-regulated. Not all genes altered by low melatonin were affected by high melatonin, indicating different roles of melatonin in regulation of plant growth and development under low and high concentrations. Furthermore, a large number of genes altered by melatonin were involved in plant stress defense. Transcript levels for many stress receptors, kinases, and stress-associated calcium signals were up-regulated. The majority of transcription factors identified were also involved in plant stress defense. Additionally, most identified genes in ABA, ET, SA and JA pathways were up-regulated, while genes pertaining to auxin responses and signaling, peroxidases, and those associated with cell wall synthesis and modifications were mostly down-regulated. Our results indicate critical roles of melatonin in plant defense against various environmental stresses, and provide a framework for functional analysis of genes in melatonin-mediated signaling pathways.

Function of the hypothalamo-neurohypophysial system in rats with myocardial infarction is modified by melatonin

BackgroundPineal and melatonin interactions with the hypothalamo-neurohypophysial system are well documented. In addition, vasopressin and oxytocin secretion are known to be part of the neuroendocrine response to chronic heart failure evoked by myocardial infarction. The present study was undertaken to evaluate the possible regulatory role of melatonin in the vasopressin and oxytocin release in rats with myocardial infarction. MethodsThe vasopressin and oxytocin content of the hypothalamus and neurohypophysis, as well as their plasma levels, were radioimmunoassayed in sham-operated or pinealectomized rats with left coronary artery ligation (CAL)-evoked myocardial infarction as well as under melatonin treatment. ResultsInfarcted rats demonstrated increased vasopressin and oxytocin plasma levels, but melatonin restricted the release of both neurohormones in these rats. Experimental myocardial infarction in pinealectomized rats caused a distinct inhibition of vasopressin release but intensified oxytocin secretion. In pinealectomized rats substituted with melatonin, pineal indole amine was seen to inhibit oxytocin release and stimulate vasopressin secretion. Conclusions(i) CAL-induced myocardial infarction is the reason for increased hypothalamo-neurohypophysial system activity in rats; melatonin plays the role of inhibitory neuromodulator of vasopressin and oxytocin release in this state. (ii) Myocardial infarction evoked in pinealectomized rats is characterized by the inversion of the neurohumoral response pattern in respect of inhibited vasopressin release. (iii) Melatonin stimulates vasopressin (but decreases oxytocin) release in pinealectomized rats with myocardial infarction.

Melatonin decreases duodenal epithelial paracellular permeability via a nicotinic receptor–dependent pathway in rats in vivo

Intestinal epithelial intercellular tight junctions (TJs) provide a rate-limiting barrier restricting passive transepithelial movement of solutes. TJs are highly dynamic areas, and their permeability is changed in response to various stimuli. Defects in the intestinal epithelial TJ barrier may contribute to intestinal inflammation or leaky gut. The gastrointestinal tract may be the largest extrapineal source of endogenous melatonin. Melatonin released from the duodenal mucosa is a potent stimulant of duodenal mucosal bicarbonate secretion (DBS). The aim of this study was to elucidate the role of melatonin in regulating duodenal mucosal barrier functions, including mucosal permeability, DBS, net fluid flux, and duodenal motor activity, in the living animal. Rats were anesthetized with thiobarbiturate, and a ~30-mm segment of the proximal duodenum with an intact blood supply was perfused in situ. Melatonin and the selective melatonin receptor antagonist luzindole were perfused luminally or given intravenously. Effects on permeability (blood-to-lumen clearance of (51)Cr-EDTA), DBS, mucosal net fluid flux, and duodenal motility were monitored. Luminal melatonin caused a rapid decrease in paracellular permeability and an increase in DBS, but had no effect on duodenal motor activity or net fluid flux. Luzindole did not influence any of the basal parameters studied, but significantly inhibited the effects of melatonin. The nonselective and noncompetitive nicotinic acetylcholine receptor antagonist mecamylamine abolished the effect of melatonin on duodenal permeability and reduced that on DBS. In conclusion, these findings provide evidence that melatonin significantly decreases duodenal mucosal paracellular permeability and increases DBS. The data support the important role of melatonin in the neurohumoral regulation of duodenal mucosal barrier.

Melatonin suppresses proinflammatory mediators in lipopolysaccharide‐stimulated CRL1999 cells via targeting MAPK, NF‐κB, c/EBPβ, and p300 signaling

Melatonin is an indoleamine secreted by the pineal gland as well as a plant-derived product that exerts potential anti-inflammatory properties, but the mechanisms of action remain unclear. Here, we investigated the roles of melatonin in regulation of proinflammatory mediators and identified the underlying mechanisms in human vascular smooth muscle (VSM) cell line CRL1999 stimulated by lipopolysaccharide (LPS). We found that treatment with melatonin significantly inhibited the production and expression of TNF-α and interleukin (IL)-1β, cyclooxygenase-2 (COX-2), inducible nitric oxide synthase, prostaglandin E(2) (PGE2), and nitric oxide (NO) in a dose-dependent manner. Moreover, we also found that the suppression of proinflammatory mediators by melatonin was mediated through inhibition of MAPK, NF-κB, c/EBPβ, and p300 signaling in LPS-stimulated CRL1999 cells. Treatment with melatonin markedly inhibited phosphorylation of ERK1/2, JNK, p38 MAPK, IκB-α, and c/EBPβ, blocked binding of NF-κB and c/EBPβ to promoters, and suppressed p300 histone acetyltransferase (HAT) activity and p300 HAT-mediated NF-κB acetylation. Transfection with an ERK-, IκB-, or c/EBPβ-specific siRNA or pretreatment with an ERK-, p38 MAPK-, or p300-selective inhibitor considerably abrogated the melatonin-mediated inhibition of proinflammatory mediators. Conversely, exogenous overexpression of a constitutively active p300, but not its HAT mutant, effectively reversed the melatonin-mediated inhibitions. Collectively, these results indicate that melatonin suppresses proinflammatory mediators by simultaneously targeting the multiple signaling such as ERK/p38 MAPK, c/EBPβ, NF-κB, and p300, in LPS-stimulated VSM cell line CRL1999, and suggest that melatonin is a potential candidate compound for the treatment of proinflammatory disorders.

Daily variation in antioxidant enzymes and lipid peroxidation in lungs of a tropical bird Perdicula asiatica: Role of melatonin and nuclear receptor RORα

The wild animals are exposed in nature to more oxidative stress than any laboratory animals. Studies on oxidative stress of brain, liver and kidney are quite common while very less reports are available on respiratory system when it is the most susceptible organ to various stressors. We checked the oxidative stress of lung tissue of a wild seasonally breeding bird Perdicula asiatica by noting down the daily variation in antioxidant enzymes (superoxide dismutase and catalase) levels, lipid peroxidation in terms of malondialdehyde level and total antioxidant status during reproductively active (RAP) and inactive phase (RIP). On the other hand melatonin has been accepted as free radical scavenger acting via receptor (nuclear receptor) or non receptor pathway. To pin point the role of melatonin in regulation of antioxidant enzymes via non receptor mediated pathway in lungs of bird, we checked variation in the nuclear melatonin receptor RORα. Antioxidant enzymes (superoxide dismutase and catalase) exhibited a marked 24h rhythm in lungs being high during night time and coincided almost with the peak of melatonin and total antioxidant status where as malondialdehyde level and nuclear receptor RORα showed inverse relationship with all the above mentioned parameters. These findings suggest that melatonin might be acting as an antioxidant for the free radical load of lung tissue of a tropical bird P. asiatica and its action might be via nuclear receptor RORα.

The central role of melatonin in Danio rerio appetite regulation

Event Abstract Back to Event The central role of melatonin in Danio rerio appetite regulation Beatrice Migliarini1*, Chiara C. Piccinetti1, Ike Olivotto1 and Oliana Carnevali1 1 Polytechnic University of Marche, Department of Marine Sciences, Italy Melatonin is the hormonal mediator of photoperiodic information to the central nervous system in vertebrates and allows the regulation of energy homeostasis through the establishment of a proper balance between energy intake and energy expenditure. The aim of this study was to evaluate the role of melatonin in the regulation of feeding behavior in the zebrafish Danio rerio. For this purpose, the effect of two different melatonin doses (100 nM and 1µM) administered for ten days via water to zebrafish adults was evaluated at both physiological and molecular level and the effect of melatonin was considered in relation to the most prominent systems involved in appetite regulation. The melatonin control of food intake by the modulation of leptin, MC4R, ghrelin, NPY and CB1 gene expression was evaluated. The results obtained indicate that melatonin significantly reduces food intake and the reduction is in agreement with the changes observed at the molecular level. A significant increase in genes codifying for molecules involved in feeding inhibition, such as leptin and MC4R, and a significant reduction in the major orexigenic signals including ghrelin, NPY and CB1 was demonstrated. These results support the idea that melatonin falls fully into the complex network of signals that regulate food intake and therefore this hormone plays a key role in central appetite regulation. Keywords: comparative endocrinology Conference: 25th Conference of the European Comparative Endocrinologists, Pécs, Hungary, 31 Aug - 4 Sep, 2010. Presentation Type: Conference Presentation Topic: Comparative endocrinology Citation: Migliarini B, Piccinetti CC, Olivotto I and Carnevali O (2010). The central role of melatonin in Danio rerio appetite regulation. Front. Endocrinol. Conference Abstract: 25th Conference of the European Comparative Endocrinologists. doi: 10.3389/conf.fendo.2010.01.00014 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 26 Aug 2010; Published Online: 29 Aug 2010. * Correspondence: Dr. Beatrice Migliarini, Polytechnic University of Marche, Department of Marine Sciences, Ancona, Italy, b.migliarini@univpm.it Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Beatrice Migliarini Chiara C Piccinetti Ike Olivotto Oliana Carnevali Google Beatrice Migliarini Chiara C Piccinetti Ike Olivotto Oliana Carnevali Google Scholar Beatrice Migliarini Chiara C Piccinetti Ike Olivotto Oliana Carnevali PubMed Beatrice Migliarini Chiara C Piccinetti Ike Olivotto Oliana Carnevali Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.

Human pineal physiology and functional significance of melatonin

Descriptions of the pineal gland date back to antiquity, but its functions in humans are still poorly understood. In both diurnal and nocturnal vertebrates, its main product, the hormone melatonin, is synthesized and released in rhythmic fashion, during the dark portion of the day–night cycle. Melatonin production is controlled by an endogenous circadian timing system and is also suppressed by light. In lower vertebrates, the pineal gland is photosensitive, and is the site of a self-sustaining circadian clock. In mammals, including humans, the gland has lost direct photosensitivity, but responds to light via a multisynaptic pathway that includes a subset of retinal ganglion cells containing the newly discovered photopigment, melanopsin. The mammalian pineal also shows circadian oscillations, but these damp out within a few days in the absence of input from the primary circadian pacemaker in the suprachiasmatic nuclei (SCN). The duration of the nocturnal melatonin secretory episode increases with nighttime duration, thereby providing an internal calendar that regulates seasonal cycles in reproduction and other functions in photoperiodic species. Although humans are not considered photoperiodic, the occurrence of seasonal affective disorder (SAD) and its successful treatment with light suggest that they have retained some photoperiodic responsiveness. In humans, exogenous melatonin has a soporific effect, but only when administered during the day or early evening, when endogenous levels are low. Some types of primary insomnia have been attributed to diminished melatonin production, particularly in the elderly, but evidence of a causal link is still inconclusive. Melatonin administration also has mild hypothermic and hypotensive effects. A role for the pineal in human reproduction was initially hypothesized on the basis of clinical observations on the effects of pineal tumors on sexual development. More recent data showing an association between endogenous melatonin levels and the onset of puberty, as well as observations of elevated melatonin levels in both men and women with hypogonadism and/or infertility are consistent with such a hypothesis, but a regulatory role of melatonin has yet to be established conclusively. A rapidly expanding literature attests to the involvement of melatonin in immune function, with high levels promoting and low levels suppressing a number of immune system parameters. The detection of melatonin receptors in various lymphoid organs and in lymphocytes suggests multiple mechanisms of action. Melatonin has been shown to be a powerful antioxidant, and has oncostatic properties as well, both direct and indirect, the latter mediated by its effects on reproductive hormones. Finally, there are reports of abnormal daily melatonin profiles in a number of psychiatric and neurological disorders, but the significance of such abnormalities is far from clear.

The effect of melatonin administration on circulating plasma luteinizing hormone concentration in castrated White Leghorn roosters

Melatonin (MLT) has a significant role in mammalian reproduction, with little or no effect in birds. In the present study we studied the role of MLT in regulation of luteinizing hormone (LH) secretion in castrated White Leghorn (WL) roosters. In Experiment 1, castrated WL roosters (n = 30) were divided into three groups, and each group (n = 10) was subdivided into two subgroups (n = 5). Birds in one subgroup received an injection of MLT at 5 mg (MLT-5), 20 mg (MLT-20), or 80 mg (MLT-80)/kg BW. Birds in the second subgroup were vehicle-injected and served as controls. Each dose of MLT was administered on a separate day at 1100 h. Blood was sampled 30 min before and 10, 30, 60, and 120 min after MLT or vehicle administration. Ten minutes after MLT administration, a significant reduction in plasma LH was observed in the MLT-20 and -80 groups, i.e., 70.3 +/− 8.3% and 62.2 +/− 4.1% of control values, respectively. In the MLT-80 group, plasma LH further declined to 42.1 +/− 9.7% of control values 60 min after injection. In Experiment 2, 18 castrated WL roosters were divided into three groups of six birds each. Two groups were injected with 80 mg MLT/kg BW at the beginning of the experiment; the second group received an additional dose of 80 mg MLT/ kg BW 140 min after the first injection. The third group was injected twice (as in second group) with vehicle and served as control. Blood was sampled 30 min before and 30, 60, 120, 170, 200, and 240 min after injection. Repeated MLT injection maintained low levels of plasma LH level until the end of the experiment. In Experiment 3, 10 castrated WL roosters were divided into two groups (n = 5). The first group was injected daily, for 10 d, with 80 mg MLT/kg BW, the second group was vehicle-treated and served as a control. At Day 3, there was a significant reduction in plasma LH level in the MLT-treated group, which continued for 7 d. This study demonstrates that, in birds, MLT suppresses LH secretion in a dose- and a time-related manner.