The effect of treadmill running exercise training on the content of some cytokines in the skeletal muscles of mice with a model of type II diabetes mellitus was studied. To develop a model of the disease, a high fat feeding for 12 weeks was used, physical activity in the form of forced running was carried out for 4 weeks. The concentration of myokines in the muscle tissue of m. gastrocnemius was determined by enzyme-linked immunosorbent assay (ELISA). The development of diabetes in mice is accompanied by an increase in the concentration of inflammatory cytokines IL-6 and a decrease in the concentration of IL-15 in skeletal muscle tissue. 4 weeks treadmill running exercise training has a different effect on the content of myokines in healthy and sick mice. In healthy animals, there was a decrease in IL-6 and IL-15 concentration and an increase in LIF concentration in skeletal muscle tissue after 4 weeks of regular forced running. At the same time, in diabetic mice, the concentrations of IL-6 and IL-15 increased after exercise, while LIF, on the contrary, decreased. The concentration of NAP3 in the skeletal muscle tissue of mice was insensitive to either the formation of diabetes mellitus II or to regular treadmill running exercise training.