Regular Sleep Schedule Research Articles

Daily Electronic Media Use and Sleep in Late Life.

Daily electronic media use, including television viewing and computer use, is common in older adulthood. Yet, increased electronic media usage may disrupt nightly sleep, leading to sleeping fewer hours and more sleep disruptions. The current study examined these relationships in older adulthood, as well as the potential buffering effect of having a regular sleep schedule. Older adults (N = 273) from the Daily Experiences and Well-Being Study (DEWS) completed 5-6 days of data collection where they answered questions at the beginning of the day about the previous night's sleep as well as questions throughout the day about daily electronic media use. They also wore Actical accelerometers to capture sleep regularity. Older adults reported sleeping fewer hours and having more sleep disturbances on days when they reported more instances of computer use. Sleep regularity moderated the daily association between TV viewing and sleep disturbances such that daily TV viewing was associated with more sleep complaints only for older adults who had less regular sleep patterns. However, sleep regularity no longer moderated this association when accounting for napping behavior. These findings provide evidence that older adults sleep worse after days when they engage in more electronic media use. The association with TV viewing with sleep disturbances on any given day is somewhat mitigated by engaging in regular sleep patterns. Researchers discuss the importance of assessing electronic media use and sleep in daily life as the role of sleep regularity may be a modifiable protective factor.

Cerebrospinal fluid mesencephalic astrocyte-derived neurotrophic factor: A moderating effect on sleep time and cognitive function

BackgroundSleeping late has been associated with cognitive impairment, and insufficient sleep can affect the secretion of feeding-related cytokines. Feeding-related cytokines may contribute to cognitive deficits resulting from delayed bedtime. Glial cell line-derived neurotrophic factor (GDNF) and mesencephalic astrocyte-derived neurotrophic factor (MANF), which are feeding-related neurotrophic factors, have been associated with improved cognitive function and neuroprotective abilities. Enhanced expression of GDNF and MANF is linked to increased energy expenditure and hyperphagia, respectively. AimsThis study aimed to investigate the association between cerebrospinal fluid (CSF) GDNF, MANF, cognition, and sleep time and to explore the moderating effects of GDNF and MANF on cognitive impairment in individuals who sleep late. MethodThis cross-sectional study included participants (mean age 31.76 ± 10.22 years) who were categorized as ≤23 o'clock sleepers (n = 66) and >23 o'clock sleepers (n = 125) based on sleep time. Cognition was assessed using Montreal Cognitive Assessment (MoCA), and GDNF and MANF levels in CSF were measured. ResultsMANF may play a moderating role in the relationship between sleep time and cognition (R2 = 0.06, β = 0.59, p = 0.031). Age showed a negative correlation with MoCA scores (R2 = 0.08, β = −0.18), while education exhibited a positive correlation (β = 0.17, both p < 0.05). Only ≤23 o'clock sleepers exhibited a negative correlation between MANF levels and BMI (r = −0.35, p = 0.005). ConclusionsThis study provides hitherto undocumented evidence of the potential protective effect of CSF MANF on cognitive impairment of late sleepers, which suggests that maintaining a regular sleep schedule may contribute to cognition and overall health, with MANF playing a role in this process.

Sleep deficiency and symptoms of pain, fatigue, and depressed mood in youth with and without childhood systemic lupus erythematosus.

We undertook a study to describe and compare sleep deficiency and symptoms of pain, fatigue, and depressed mood in youth with childhood systemic lupus erythematosus (cSLE) to a healthy comparison group of youth and test the associations between sleep and symptoms of pain, fatigue, and depressed mood in youth with cSLE. Forty-three youth (23 youth with cSLE; 20 age- and sex-matched healthy youth) wore actigraphs and completed sleep diaries for 10 days and completed self-report questionnaires on sleep quality, pain, fatigue, and depressed mood. On average, both groups had a total sleep time of less than 7 hours. Youth with cSLE had worse sleep efficiency (73.3%) and sleep regularity index scores (55.4) compared to the healthy comparison group of youth (79.2% and 60.1, respectively). Youth with cSLE had worse pain (P = .03) and fatigue (P = .004) compared to the healthy comparison group. Negative associations were found among self-reported sleep quality, sleep satisfaction, and symptoms of pain, fatigue, and depressed mood in youth with cSLE and wake after sleep onset was positively associated with fatigue. Poor sleep efficiency and sleep irregularity accompanied by symptoms of pain, fatigue, and depressed mood was prevalent in youth with cSLE. Youth with lupus should be encouraged to maintain a regular sleep schedule. Because this is the first study to incorporate objective sleep and sleep regularity measures in youth with cSLE, additional studies with objective and self-reported sleep measures are needed to replicate our findings. Wi D, Palermo TM, Stevens A, Ward TM. Sleep deficiency and symptoms of pain, fatigue, and depressed mood in youth with and without childhood systemic lupus erythematosus. J Clin Sleep Med. 2024;20(10):1607-1614.

Sleep/wake regularity influences how stress shapes executive function

IntroductionSleep and stress processes shape executive function. Evidence suggests that poor sleep regulation can lead to significant impairments in executive functions. Psychological stress can also directly impact a variety of executive functions, often leading to declines, but may additionally reduce executive function via its negative impact on sleep. Rates of perceived stress and poor sleep have skyrocketed in recent years. As such, it has become increasingly important to understand how daily stress exposures and sleep processes modulate executive functions.MethodsIn a remote 21-day app-based study, 227 participants completed sleep and stress assessments three times a day. They also completed three executive functioning tasks at various timepoints across the 21-day study interval that assessed cognitive inhibition (Emotional Stroop task), cognitive flexibility (Trail Making Test A and B), and working memory (Backwards Digit Span).ResultsParticipants with consistent sleep/wake schedules reported fewer acute stress events when compared to those with inconsistent sleep schedules. Those with greater sleep/wake regularity also had faster responses to self-relevant negative probes (vs. general negative and neutral) in the Stroop task. Further, variability in sleep/wake timing and reported acute stress exposures across the 21-day study interval interacted to predict performance on the Emotional Stroop task. Specifically, as the number of acute stress events experienced across the 21-day interval increased, participants with more regular sleep schedules had slower overall response times on the Stroop. Higher acute stress exposures led to specific response time delays to neutral and self-relevant negative probes for those with high sleep/wake regularity. We found no impact of the number of acute stress events or stress intensities on working memory span, Stroop accuracy, or Trails response time.DiscussionThese data may indicate that sleep/wake regularity preserves adaptive inhibitory control responses to cumulative acute stress.

Secretion of glucagon, GLP-1 and GIP may be affected by circadian rhythm in healthy males.

Glucagon is secreted from pancreatic alpha cells in response to low blood glucose and increases hepatic glucose production. Furthermore, glucagon enhances hepatic protein and lipid metabolism during a mixed meal. Glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP) are secreted from gut endocrine cells during meals and control glucose homeostasis by potentiating insulin secretion and inhibiting food intake. Both glucose homeostasis and food intake have been reported to be affected by circadian rhythms and vice versa. In this study, we investigated whether the secretion of glucagon, GLP-1 and GIP was affected by circadian rhythms. A total of 24 healthy men with regular sleep schedules were examined for 24h at the hospital ward with 15h of wakefulness and 9h of sleep. Food intake was standardized, and blood samples were obtained every third hour. Plasma concentrations of glucagon, GLP-1 and GIP were measured, and data were analyzed by rhythmometric statistical methods. Available data on plasma glucose and plasma C-peptide were also included. Plasma concentrations of glucagon, GLP-1, GIP, C-peptide and glucose fluctuated with a diurnal 24-h rhythm, with the highest levels during the day and the lowest levels during the night: glucagon (p < 0.0001, peak time 18:26h), GLP-1 (p < 0.0001, peak time 17:28h), GIP (p < 0.0001, peak time 18:01h), C-peptide (p < 0.0001, peak time 17.59h), and glucose (p < 0.0001, peak time 23:26h). As expected, we found significant correlations between plasma concentrations of C-peptide and GLP-1 and GIP but did not find correlations between glucose concentrations and concentrations of glucagon, GLP-1 and GIP. Our results demonstrate that under meal conditions that are similar to that of many free-living individuals, plasma concentrations of glucagon, GLP-1 and GIP were observed to be higher during daytime and evening than overnight. These findings underpin disturbed circadian rhythm as a potential risk factor for diabetes and obesity. ClinicalTrials.gov Identifier: NCT06166368. Registered 12 December 2023.

Migraine Management Using Feverfew, Butterbur, Peppermint, and Ginger: Perspectives of Pharmacy Students

A literature search and a survey of pharmacy students were conducted regarding some common herbs used for the treatment of migraine. The literature search shows that feverfew, traditionally used for migraines, had mixed evidence of effectiveness, while butterbur received a strong recommendation for prevention. Peppermint showed promise in reducing headache intensity, particularly when given intranasally, but ginger's efficacy remains unclear. Further research is needed to confirm ginger's role in migraine treatment. The survey, which was conducted among 39 pharmacy students on five knowledge-based and five opinion-based questions regarding the use of herbal remedies revealed a varied comprehension level, with an overall 69% accuracy in knowledge-based responses regarding migraines. For the most part, respondents demonstrated awareness of lifestyle measures in preventing migraines, but many respondents lacked clarity on self-medicating with herbal remedies and over the counter products. Opinion-based inquiries indicated a positive inclination towards herbal remedies, with more than 80% agreeing on their viability as alternatives to conventional medications. The survey showed a 69% average correctness rate for knowledge-based questions about migraines. Maintaining a regular sleep schedule received the highest accuracy at 94.1%, while stress as a trigger for migraines received a 71.4% correct response rate. Over 70% believed that those using herbal remedies for migraines were generally more satisfied with their treatment outcomes. While there is a need for improved education on migraine-related topics, there is a prevailing positive attitude toward the potential benefits of herbal remedies in managing migraines. Diverse opinions existed on satisfaction levels and the comparative effectiveness of lifestyle changes versus herbal interventions. Despite limitations in the sample size and focus on pharmacy students, the study underscores the need for enhanced education on migraines while highlighting growing interest in alternative healthcare among healthcare professionals.

Unveiling the Shadows: The Alarming Link Between Sleep Irregularity and Dementia

Nature has beautifully planned nocturnal hours during which neurobiological processes critical for the preservation of cognitive health and prevention of dementia take place. Irregular sleeping may interfere with the brain’s function of eliminating neurotoxic, and results in onset of neurodegeneration. Studies have revealed a surprising relationship between irregular sleeping and dementia. &#x0D; Dementia is known to be major cause of disability and mortality worldwide. According to WHO, estimated 55 million individual suffering with dementia globally and this number is expected to raise up to 140 million in 2050 [1]. A variety of neurodegenerative diseases comes under dementia, and they are all characterized by a loss of cognitive abilities including memory, language, problem-solving, and the capacity to carry out daily tasks. Alzheimer disease is the most common form and may contribute to 60–70% of all cases. It is due to accumulation of beta-amyloid. &#x0D; Irregular sleeping has become a widespread problem that is silently draining millions of people's cognitive capacities and may even be the precursor to dementia. Findings have shown U-shaped relationship between sleep regularity and dementia risk. Very irregular and overly consistent sleep patterns, both are linked to a higher risk of dementia. &#x0D; Previous studies have shown the association between irregular sleeping and dementia but does not prove that dementia is caused by irregular sleep. Future studies, including longer follow-up periods and inquiries into the underlying molecular mechanisms, are required to better understand the complicated link between sleep regularity and dementia. It would be helpful to conduct further intervention trials aimed at improving irregular sleep in individuals with highly variable sleep patterns in order to ascertain whether restoring regular sleep patterns actually enhances brain health. &#x0D; Currently, there is no treatment available for any of the prevalent form of dementia, including frontotemporal dementia, Alzheimer's disease, vascular dementia, and Lewy body dementia. Individual with dementia can preserve the quality of life and well-being by engaging in physical activity and by maintaining the social connections that stimulate brain functioning. Some tips can be followed to improve sleep, such as maintaining a regular sleep schedule or abstaining from caffeine and stimulating screen time 1-2 hours before bed or by consulting your doctor for assistance and to discuss your sleep issues, habits, and general health. &#x0D; In summary, the connection between sleep disorders and dementia serves as a warning, drawing attention to a frequently overlooked aspect that may be essential to prevent a wave of subsequent cognitive deterioration.

HUBUNGAN KRONOTIPE DENGAN DERAJAT NYERI DISMENORE PADA REMAJA PUTRI

Dysmenorrhea is one of the most common menstrual disorders in young women. The prevalence of dysmenorrhea in adolescent girls in Indonesia ranges from 16.8-81%. Girls with dysmenorrhea symptoms have a higher risk of decreased activity, experiencing hip strain, back pain, headaches, and fatigue. In school children, it can interfere with learning activities and concentration, causing academic decline. Dysmenorrhea is influenced by several factors from daily activities and habits. Activity can affect a person's sleep and wake schedule. The difference in sleep and activity schedules of each person is known as kronotipe. The study aimed to determine the relationship between kronotipe and the degree of dysmenorrhea pain in adolescent girls at SMKN 1 Doko Blitar. The research was conducted in August-September 2021. The population of the study was 60 students of SMK 1 Doko in Blitar district aged 15-18 years old, non-smoking, and who want to join this study. The research plan is a cross-sectional study using MEQ-SA (Morningness-Eveningness Questionnaire Self-Assessment Version), and the dysmenorrhea questionnaire. Data analysis uses the chi-square test. The result of chi-square test showed a significant correlation between the kronotipe with the degree of dysmenorrhea pain (p-value= 0,000). There is a significant relationship between chronotype and the degree of dysmenorrhea pain. Adolescent girls need to set a regular sleep schedule to have an appropriate chronotype to reduce dysmenorrhea pain during menstruation.

Light exposure during sleep is bidirectionally associated with irregular sleep timing: The multi-ethnic study of atherosclerosis (MESA)

Exposure to light at night (LAN) may influence sleep timing and regularity. Here, we test whether greater light exposure during sleep (LEDS) is bidirectionally associated with greater irregularity in sleep onset timing in a large cohort of older adults in cross-sectional and short-term longitudinal (days) analyses. Light exposure and activity patterns, measured via wrist-worn actigraphy (ActiWatch Spectrum), were analyzed in 1933 participants with 6+ valid days of data in the Multi-Ethnic Study of Atherosclerosis (MESA) Exam 5 Sleep Study. Summary measures of LEDS averaged across nights were evaluated in linear and logistic regression analyses to test the association with standard deviation (SD) in sleep onset timing (continuous variable) and irregular sleep onset timing (SD > 90 min, binary). Night-to-night associations between LEDS and absolute differences in nightly sleep onset timing were also evaluated with distributed lag non-linear models and mixed models. In between-individual linear and logistic models adjusted for demographic, health, and seasonal factors, every 5-lux unit increase in LEDS was associated with a 7.8-min increase in sleep onset SD (β = 0.13 h, 95%CI:0.09–0.17) and 32% greater odds (OR = 1.32, 95%CI:1.17–1.50) of irregular sleep onset. In within-individual night-to-night mixed model analyses, every 5-lux unit increase in LEDS the night prior was associated with a 2.2-min greater deviation of sleep onset the next night (β = 0.036 h, p < 0.05). Conversely, every 1-h increase in sleep deviation was associated with a 0.35-lux increase in future LEDS (β = 0.348 lux, p < 0.05). LEDS was associated with greater irregularity in sleep onset in between-individual analyses and subsequent deviation in sleep timing in within-individual analyses, supporting a role for LEDS in irregular sleep onset timing. Greater deviation in sleep onset was also associated with greater future LEDS, suggesting a bidirectional relationship. Maintaining a dark sleeping environment and preventing LEDS may promote sleep regularity and following a regular sleep schedule may limit LEDS.

Effect Of Progressive Muscle Relaxation On Insomnia Among The Elderly: A Literature Review

Introduction: The term "elderly" refers to a stage of human existence characterized by a decline in the body's capacity to respond to external stress and a failure to maintain body equilibrium in physiological stress situations. Patients who are elderly are more likely to suffer from sleep difficulties, including insomnia, which is one of the effects they experience. Insomnia is a sleep disorder defined by a lack of capacity to establish or sustain regular sleep schedules; symptoms usually appear within a week. Material and Methods: This research is a review of the existing literature. The topic, articles from 2018 to 2023, were chosen from various online databases, including Science Direct, PubMed, and Google Scholar. The PRISMA approach was utilized by analyzing the literature review. Results: After sifting through 315 publications, only 16 studies were deemed suitable for analysis. The conclusion drawn from these studies is that PMT significantly decreases the prevalence of insomnia in the elderly. Conclusion: Progressive Muscle Relaxation has been shown in various studies to alleviate insomnia in the elderly.

Self-reported sleep status and influencing factors: a web-based national cross-sectional survey in China

Objectives To investigate self-reported sleep duration, sleep timing, sleep status and influencing factors in the Chinese population. Methods This web-based cross-sectional survey was conducted in 2022, covering 31 provinces (91%) in China. 11,000 questionnaires were collected, of which 8970 were valid for analysis. Self-reported sleep habits, problems and quality were investigated. Good or fair sleep ratings, enough duration, regular, with no sleep disturbances and <30 min sleep latency was defined as a composite variable: ‘Good sleep’. Factors influencing sleep patterns and ‘Good sleep’ were analyzed by multivariate logistic regression. Results Most participants sleep less than 7 h per night (55.13%), usually go to bed at 10–12 pm (47.99%), wake up at 6–8 am (49.86%), and take less than 30 min to fall asleep (66.30%) with regular sleep schedule (76.01%). Only 12.36% have ‘Good sleep’. In the past 3 months, 46.80% of the participants reported symptoms of insomnia, and 21.54% had snoring problems. Among the adults, the young, males, college students, freelancers, and those who resided in urban areas and pandemic-free areas slept later, and the northerners woke up earlier. The adults with low-moderate and moderate income and the minors at elementary and middle school slept earlier and woke up earlier. Mid-aged adults who often napped at noon were more likely to have ‘Good sleep’ than any other age group, and urban dwellers with the same habit were more likely to have ‘Good sleep’ than people dwelled in other regions. While people who slept late, woke up too early or too late, slept too little or too much, resided at GMT 7–8 area or pandemic area, had high income, or took up some occupations (entrepreneurs/individuals, professionals, manual and non-manual workers, housewives) were less likely to get a ‘Good sleep’. Conclusions The national survey provided a sleep profile of the Chinese population. Both socio-economic status and personal sleep hygiene habits had an impact on ‘Good sleep’.

Insomnia and Cardiovascular Health: Exploring the Link Between Sleep Disorders and Cardiac Arrhythmias.

Cardiovascular diseases (CVDs), driven by modern lifestyles, have increased, with atrial fibrillation (AF) being a major concern linked to heart failure and stroke. Insomnia affects a large population, especially younger individuals, males, and menopausal women, decreasing the quality of life and potentially causing autonomic disturbances and cardiac arrhythmias. PURPOSE OF REVIEW: This review explores the link between insomnia and cardiac arrhythmias, particularly AF, and its impact on cardiovascular health and emphasizes the need to address insomnia in individuals with cardiac arrhythmias by tailored strategies for sleep management to improve their overall well-being. RECENT FINDINGS: Recent findings emphasize maintaining a regular sleep schedule to lower AF and bradyarrhythmia risks. Better sleep scores correlate with reduced AF and bradyarrhythmia risks, while insomnia increases AF risk, particularly in those under 40years of age. Studies underscore the potential impact of sleep management in reducing cardiovascular risks and highlight the importance of addressing sleep issues to improve cardiovascular health outcomes. Our review presents compelling evidence connecting insomnia and AF. Improving sleep patterns and addressing sleep issues can reduce AF risk, benefiting cardiovascular health. A comprehensive approach for managing at-risk individuals with cardiac arrhythmias, considering co-existing conditions, can decrease long-term disease burden and expenses. Incorporating sleep assessments and interventions into cardiovascular risk management, especially for those with insomnia, is recommended. Further research is needed to fully comprehend the complex relationship between insomnia and cardiac arrhythmias.

Sleeping and Dietary Factors Associated with Chronic Fatigue Syndrome in Taiwanese Preschoolers.

The purpose of this research was to investigate the sleeping and dietary factors associated with the prevalence of chronic fatigue syndrome among Taiwanese preschoolers. Five-year-old preschoolers were randomly selected using a stratified multistage random cluster sampling method. The parents of the preschoolers completed a questionnaire containing items related to symptoms of fatigue and sleeping and dietary habits among the preschoolers. A total of 1536 valid questionnaires were returned. After obtaining the data, the researchers analyzed them using descriptive statistics and a chi-square test. The following results were obtained: (1) chronic fatigue syndrome was typically indicated by yawning during the day, feeling tired, and appearing sleepy; (2) the preschoolers with high sleep quality, adequate sleeping time, and a regular sleep schedule exhibited a lower degree of fatigue; (3) half of the preschoolers who ate three nutritionally balanced meals a day at a regular time exhibited a lower degree of fatigue. Among the three dimensions studied, fatigue was most strongly associated with the "sleepy and inactive/blunted responses/lacking in energy" dimension, followed by the "difficulty concentrating" dimension, and, finally, the "localized pain" dimension. In this study, the association between sleeping habits and symptoms of fatigue in preschool children was verified. The associations of dietary factors with symptoms of fatigue were not confirmed. It is suggested that parents establish a good sleep schedule for preschool children based on the study findings.

Analysis of the Relationship between Sleep Environment and Sleep Quality in Children with Cancer in the Chemotherapy Phase

The study was conducted to analyze the relationship to find out whether there is a correlation between the sleep environment and sleep quality of children with cancer in the chemotherapy phase. The results of this study will help determine the steps to be taken to improve the bed environment and improve the quality of sleep of children with cancer in the chemotherapy phase. In this study, the method that will be used to conduct research is quantitative research, because it tests hypotheses using certain statistical analysis tools. Quantitative data is statistical data in the form of numbers, both directly extracted from research results and the results of processing qualitative data into quantitative data. This study found that sleep environment and children's sleep quality have a significant relationship. The results show that a good sleep environment, including a comfortable temperature, low noise, and dim lighting, contributes to better sleep quality in children. In addition, other factors such as the comfort of the bed and a regular sleep schedule also affect the quality of a child's sleep.

Symptom: Vertigo After Labyrinthectomy

Symptom: Vertigo After Labyrinthectomy

Beliefs about Healthy Sleep Habits in Adults with Diabetes Compared to Adults without Diabetes: A Reasoned Action Approach Elicitation Study.

Objective Sleep is part of a healthy lifestyle and in adults with diabetes, inadequate sleep is associated with risks of developing complications. The objective was to compare beliefs about healthy sleep habits (HSHs) in adults with versus without diabetes based on the Reasoned Action Approach. Methods A total of 56 adults with and 98 without diabetes answered open-ended questions regarding their beliefs about: avoiding screen use in bed; having a regular sleep schedule; or avoiding caffeine, alcohol, and cigarettes before bedtime. A qualitative content analysis was used to identify the most important beliefs, similarities, and differences between both groups. Results Both groups reported that adopting HSHs could improve sleep. Having a regular sleep schedule was perceived to facilitate diabetes management in adults with diabetes. Negative consequences specific to adopting each HSH were identified in both groups. Adopting HSHs was associated with mainly negative emotions (e.g., stress, anxiety, fear) in both groups. Avoiding screen use in bed was associated with anxiety of not knowing blood glucose levels at night in adults with diabetes. Partners, parents, and friends were considered the most important individuals who would approve of adopting HSHs, but they were often perceived as unlikely to adopt HSHs themselves in both groups. Adults with diabetes perceived more barriers to adopting HSHs. Facilitating factors for both groups included removing triggers of unhealthy sleep habits, behavior substitution, using reminders, time management, and social support. Discussion These beliefs can guide the development of behavioral sleep interventions, including interventions specifically for adults with diabetes.

P072 Circadian misalignment by simulated night shifts decreases colonoid barrier integrity in Ulcerative Colitis

Abstract Background Circadian rhythms orchestrate multiple biological processes with 24-hour oscillations that regulate diverse bodily functions. The central clock is in the suprachiasmatic nucleus and orchestrates peripheral circadian clocks in every organ system including the gastrointestinal tract (GIT). Previous work has shown that circadian misalignment in Ulcerative Colitis (UC) is associated with increased colonic permeability, and colonic organoids recapitulate the host circadian phenotype. The goals of this study were to examine whether circadian misalignment would disrupt the colonic barrier integrity in UC. Methods 5 subjects, 2 UC and 3 Healthy Controls (HC), were recruited into the study. All UC subjects were inactive (partial Mayo Score ≤ 1) and stable medications with no flares for the last 3 months. For two weeks prior to entry into the circadian lab all subjects were on a prescribed regular sleep schedule. Subjects were then kept in the circadian lab for 6 days with strict control of their light/dark cycle (Figure 1). All subjects then underwent two unprepped flexible sigmoidoscopies at: (1) baseline with circadian alignment and (2) after three days of simulated night shifts with circadian misalignment. Colonic organoids generated created from the colon tissue biopsies grown in Matrigel and barrier integrity was assessed in apical-out colonoids by FITC-Dextran (4 kDa) Net Fluorescence was estimated by ImageJ. Results Colonic organoids at baseline had significantly decreased ZO-1 in UC versus HC, Figure 2. At baseline there was an increase in UC colonic organoid barrier integrity as measured by net fluorescence (density/area). In UC vs HC barrier integrity was 855 ± 203.8 vs 333.1 ± 52.9, p&amp;lt;0.05. After three days of simulated night shifts, barrier integrity significantly increased in both groups at was 1100 ± 342 vs 656 ± 197.9,p &amp;lt;0.5 (Figure 3). After 24 hours of treatment with cytokines, barrier integrity in HC, UC, misaligned HC, and misaligned UC was 2227 ± 376, 2030 ± 264, 1787 ± 402, and 1506 ± 332, (Figure 4). Conclusion Circadian misalignment by alternation of light:dark cycles (simulated night shift) causes misalignment of host central clock and impacts circadian regulated biological process like tight junction proteins which decreases colonic barrier integrity. This study highlights the importance of studying environmental factors that impact circadian timing in UC including night shift work which may be a significant risk factor for a disease flare. Further in vivo and in vitro studies are ongoing to determine the mechanisms through which circadian misalignment impacts colonic barrier integrity in UC (NCT05180279).

Symptoms: Sudden Hearing Loss and Aural Fullness

Symptoms: Sudden Hearing Loss and Aural Fullness

O061 Sleep hygiene guidelines for shift workers: consensus guidelines via a Delphi study

Abstract Introduction Current sleep hygiene guidelines are impractical for shift workers, given their inability to maintain regular sleep schedules. Further, the present guidelines contradict fatigue risk management advice (e.g., advising against caffeine and daytime napping). The aim of the present study is to develop a set of tailored sleep hygiene guidelines for shift workers via a Delphi approach. Methods Draft guidelines were developed by the research team, who reviewed the current guidelines and examined existing evidence on sleep hygiene in shift workers. Experts from sleep, shift work, and occupational health fields (n=155) were invited to review these draft guidelines via a three-round series of online questionnaires. In each round, experts voted on each guideline, with consensus reached once there was 70% agreement on including the item as written. Expert feedback from each round was discussed and incorporated by the research team as appropriate. Results The research team drafted 17 sleep hygiene recommendations, covering sleep scheduling, napping, sleep environment, substance consumption, diet, and exercise. Of those experts invited, 68 (44%) agreed to participate, with 55 of these (81%) completing all three rounds. Consensus was reached on all original items after three rounds, with two additional items (medication use and sleep inertia) and an introductory statement developed, to create a final set of guidelines with 19 items. Discussion This is the first study to create evidence-based, expert-developed sleep hygiene guidelines for shift workers. Future research is planned to investigate the acceptability and implementation of the guidelines amongst end-users.

0184 Too Jittery to Sleep? Temporal Associations of Actigraphic Sleep and Caffeine in Adolescents

Abstract Introduction The majority of adolescents report consuming a caffeinated beverage on a typical day, which has been linked to poor sleep health in cross-sectional studies. However, it is unknown whether poor sleep predicts caffeine consumption, and/or whether caffeine consumption predicts poor sleep, particularly when sleep is measured objectively. The current study examined within- and between-person associations of actigraphic sleep dimensions with caffeinated beverage consumption in adolescents. Methods Data were collected from a micro-longitudinal substudy of the age 15 wave of the Fragile Families and Child Wellbeing Study (n=589). Adolescents wore a wrist-actigraphy device and completed daily surveys for approximately one week (mean=5.6 days). Daily surveys assessed sleep quality and caffeinated beverage consumption (0=no caffeine, 1=any caffeine). Separate mixed models assessed whether actigraphy-measured sleep duration, timing, maintenance efficiency, and subjective quality predicted next-day caffeinated beverage consumption within and between adolescents. Variability of sleep duration and timing (SD), sleep regularity index, and social jetlag were tested as additional between-person predictors. Lagged models tested whether daily caffeinated beverage consumption predicted sleep that night (n=458; mean=5.2 days). Results Between-person results showed that adolescents who had more variable actigraphic sleep duration (OR=1.21, p=.042) and sleep midpoint (OR=1.27, p=.045) had greater odds of consuming caffeinated beverages compared to others. Within-person results showed that on days when adolescents consumed ≥1 caffeinated beverage, they had later sleep onset by (b±SEM) 17±6 mins (p=.003) that night and later wake time by 19±7 mins (p= .011) the next morning, compared to days when they did not consume caffeine. Sleep duration, timing, maintenance efficiency, and subjective quality did not predict next-day caffeinated beverage consumption (all p&amp;gt;.10). Conclusion Greater variability in sleep duration and timing and later sleep timing are risk factors for poor emotional and cardiometabolic health. Curbing caffeinated beverage consumption may aid in the maintenance of regular sleep schedules and advance sleep timing in adolescents, potentially improving physical and psychological health. Support (If Any) R01HD073352