Region Southern Italy Research Articles

Stachys italica Mill.: synecology, functional compounds and potential use of an Italian endemic taxon.

The metabolomic of the Italian endemic species Stachys italica was investigated and potential positive metabolites for human's health were detected, quantified and discussed in relation to its synecology. Stachys italica is a species endemic to central-southern Italy, traditionally used for human consumption. The present research reports the results of a phytosociological study of this species in two southern regions of Italy (Apulia and Calabria). The collected plant material was used to make two types of extracts: hot water infusion to evaluate the use of this plant as tea and hydroalcoholic extraction to evaluate the use of it in herbal liqueur preparation. The extracts obtained by the hot water infusion had a values of total polyphenols and antioxidant capacity similar to values found in black tea. The analysis of non-volatiles compounds revealed the presence of biologically active substances like lavandulifolioside, verbascoside, and methoxyflavones. The analysis of volatiles fraction of metabolites demonstrated fifty-three volatiles compounds in the plant aerial part, 19 of which belonging to monoterpenoids, and 17 to the class of sesquiterpenoids. Among them, α-pinene, β-pinene, cis-ocimene, limonene and t-caryophyllene, were the most abundant compounds. The results were compared with already published results and referred to other similar species (such as Sideritis syriaca) which represent herbal mixtures usually collected and used as "mountain tea" in the Balkans and Eastern European countries.

Age Differences and Prevalence of Comorbidities for Death and Survival in Patients with COVID-19: A Single-Center Observational Study in a Region of Southern Italy

The SARS-CoV-2 outbreak has resulted in a considerable number of deaths worldwide. The virus damages the pulmonary artery endothelium, leading to a condition known as microvascular pulmonary inflammatory thrombotic syndrome (MPITS), which can be fatal and cause multiple organ failure. The presence of preexisting comorbidities has been shown to significantly impact the severity and prognosis of patients with SARS-CoV-2 infection. The objective of this study was to compare the age groups of patients with coronavirus disease 2019 (COVID-19) and to identify the prevalence of comorbidities associated with death and survival in an area of southern Italy. The data set consisted of 1985 patients with confirmed cases of SARS-CoV-2 infection who were admitted to the A.O.U. San Giovanni di Dio e Ruggi d’Aragona Hospital in Salerno between January 2021 and December 2022. The results were presented for the overall population and stratified by outcome and age group. All analyses were performed using the XLSTAT (Lumivero, 2024, Paris, France) and STATA software (release 16.1, StataCorp LLG, College Station, TX, USA, 2019) packages. In the study, population, 636 cases (32%) resulted in death, with a higher prevalence in the 60–79 age group, followed by the ≥80 and 30–59 age groups. The most prevalent diseases among deceased and surviving patients with confirmed cases of SARS-CoV-2 infection were those affecting the circulatory system (61.5% vs. 55.5%), the respiratory system (55.8% vs. 26.2%), and the metabolic system (25.9% vs. 25.4%). In patients aged 30–79, respiratory diseases were the primary cause of mortality, whereas in those aged ≥80, circulatory system diseases were more prevalent. Among survivors, cardiovascular diseases were the most common comorbidities across all age groups, followed by respiratory diseases and endocrine, metabolic, and immune disorders. Moreover, these comorbidities were associated with an elevated risk of mortality. The study emphasizes the substantial influence of age and comorbidities on the mortality associated with SARS-CoC-2 infection. These findings highlight the necessity for targeted interventions to manage comorbid conditions in patients with SARS-CoV-2 infection, particularly in older adults.

From One Heath to One Sustainability: The Role of Contagious Mastitis Pathogens in Decreasing the Dairy Herd Sustainability.

Economic, social, and environmental sustainability are the results of efforts aiming to improve all aspects of milk production, respecting animal welfare and improving herd health. An epidemiological study was designed to assess the role of contagious pathogens (S. aureus and S. agalactiae) in a cohort of 120 dairy herds located in the southern regions of Italy. Milk quality was assessed using certified methods, and the prevalence of mastitis pathogens in bulk tank milk was determined using quantitative polymerase chain reaction. Welfare scores were assessed using a scoring card that has more than 100 items, including animal-based measurements. Statistical analyses were performed using general lineal model and logistic regression procedures. The results showed that S. aureus had a significant negative effect on the amount of milk nutrients delivered to the dairy plant, and on the level of welfare, whereas the presence of S. agalactiae did not show any significant association. The major risk factors associated with the presence of S. aureus were also identified to help prioritize control programs. These results support the "One Sustainability" approach, implying that an increase in animal productivity is related to the improvement of animal health and welfare and potentially leading to the mitigation of greenhouse gas emissions.

Anthropogenic and climate change-driven coastline dynamics will erode future nesting habitats of the kentish plover on the central adriatic coast

Shorebirds play a crucial role in assessing the health of coastal ecosystems due to their life history, behaviour, migratory patterns, and feeding preferences. Many shorebird species are experiencing population declines worldwide, driven by habitat loss and climate change. Understanding these challenges requires extensive data on their distribution and breeding ecology. The Kentish Plover (Anarhynchus alexandrinus) is a small shorebird that constructs its nests on sandy beaches. However, there is a scarcity of data regarding the reproductive ecology of this species in many areas, including the Molise region of Southern Italy. This study aimed at assessing the potential role of environmental variables derived from remote sensing to model current and future Kentish Plover nesting habitat suitability accounting for future scenarios of coastal dynamics in 2040. Data on Kentish Plover nests were collected along the Molise coast over several years and used to calibrate ecological niche models (ENMs) relying on environmental variables derived from coastal images. Kentish Plover nesting habitat suitability was mostly affected by distance from open sand, artificial surfaces, shoreline, and herbaceous vegetation and forested areas. The study predicts an average decline of more than 22% in suitable nesting environments for the Kentish Plover within the next two decades. Results indicated that our modelling approach can offer valuable insights for the conservation strategy of Kentish Plover in the region. In conclusion, we assert that preserving the nesting habitat of the Kentish Plover will not only safeguard this species, but also other species that inhabit similar environments, along with providing protection for coastal landscapes and associated ecosystem services.

Blooming Phenograms, Pollen Production, and Pollen Quality during Storage of Pistachio Cultivars in New Mediterranean Growing Areas.

Pistachio (Pistacia vera L.) is a dioecious, anemophilous, and drought-resistant fruit tree species. It is cultivated in new Mediterranean areas, including the regions of southern Italy (Apulia and Basilicata). It has been estimated that over 40,000 ha are suitable for pistachio cultivation in areas infected by Xylella fastidiosa subsp. pauca. As a newly introduced species, knowledge of its biological reproductive behaviors in its new areas of spreading is essential for appropriate agronomic planning and management. This two-year study (2022 and 2023), carried out in the countryside of Stigliano (MT, Italy), had the objective of evaluating the flowering phenograms, pollen production, and assessing protocols for the conservation and extension of pollen viability, of the most widespread cultivars. A slight delay was observed in the blooming phenograms, compared to other cultivation Mediterranean areas, such as Spain or Sicily. Furthermore, the overlap between female and male phenograms was partial. No significant differences were observed in the polliniferous aptitude of the two male cultivars. Among the different protocols tested, the pollen storage at 33% relative humidity and a temperature of -80 °C maintained the pollen germinability above 50% for up to three weeks. These findings highlight the importance of controlled environmental conditions in preserving pollen viability over extended periods, providing valuable insights for agricultural and botanical research that relies on maintaining pollen viability for breeding and genetic studies.

Checklist of Ornamental Trees, Shrubs, and Succulents of Apulia (Southern Italy).

In this study we focused on the need to fill a knowledge gap among Italian botanical studies namely that of ornamental species census. In particular, we addressed one of the regions in southern Italy with less knowledge in the field of such studies and with an obvious presence of non-native species. A widespread census of the Apulian territory was carried out between 2021 and 2024 in both urban and suburban areas including street trees, parks, and private and historic gardens. The inventory of ornamental trees, shrubs, and succulents of Apulia (southern Italy) was carried out in six provinces, i.e., Bari, Barletta-Andria-Trani, Brindisi, Foggia, Lecce, and Taranto. The checklist comprises 287 taxa (including 265 species, 6 varieties, 5 subspecies, and 11 forms) included in 179 genera belonging to 78 families. We evaluated the number of taxa per families and genera, the presence of each taxa in the provinces of Apulia, the number of taxa per occurrence status, growth forms, geographical origin, and the number of native and alien taxa and also the artificial hybrids. Remarks of the most significant taxa and evaluation of geographical distribution in Italy were also taken into consideration. A large number of surveyed taxa (51.74%) are comprised in the list reported in the recent study on allochthonous vascular flora in Italy with a marked prevalence of Neophyte Casual Alien and Neophyte Naturalized Alien species.

Assessment of Fatty Acid and Oxylipin Profile of Resprouting Olive Trees Positive to Xylella fastidiosa subsp. pauca in Salento (Apulia, Italy).

Xylella fastidiosa subsp. pauca ST53 (XFP), the causal agent of olive quick decline syndrome (OQDS), was thoroughly investigated after a 2013 outbreak in the Salento region of Southern Italy. Some trees from Ogliarola Salentina and Cellina di Nardò, susceptible cultivars in the Gallipoli area, the first XFP infection hotspot in Italy, have resprouted crowns and are starting to flower and yield fruits. Satellite imagery and Normalized Difference Vegetation Index analyses revealed a significant improvement in vegetation health and productivity from 2018 to 2022 of these trees. Lipid molecules have long been recognized as plant defense modulators, and recently, we investigated their role in XFP-positive hosts and in XFP-resistant as well as in XFP-susceptible cultivars of olive trees. Here, we present a case study regarding 36 olive trees (12 XFP-positive resprouting, 12 XFP-positive OQDS-symptomatic, and 12 XFP-negative trees) harvested in 2022 within the area where XFP struck first, killing millions of trees in a decade. These trees were analyzed for some free fatty acid, oxylipin, and plant hormones, in particular jasmonic and salicylic acid, by targeted LC-MS/MS. Multivariate analysis revealed that lipid markers of resistance (e.g., 13-HpOTrE), along with jasmonic and salicylic acid, were accumulated differently in the XFP-positive resprouting trees from both cultivars with respect to XFP-positive OQDS symptomatic and XFP-negative trees, suggesting a correlation of lipid metabolism with the resprouting, which can be an indication of the resiliency of these trees to OQDS. This is the first report concerning the resprouting of OQDS-infected olive trees in the Salento area.

SUSCEPTIBILITY OF PHILAENUS SPUMARIUS (RHYNCHOTA, APHROPHORIDAE) TO ENTOMOPATHOGENIC NEMATODES AND FUNGI

Xylella fastidiosa, the causal agent of the devastating “olive quick decline syndrome”, is a xylem-inhabiting gram-negative bacterium that was introduced into the Apulia region of southern Italy. Xylella fastidiosa caused an epidemic outbreak and catastrophic dieback of olive groves. This pathogen is transmitted exclusively by xylem fluid-feeding sap insects, the main vector being the meadow jumping beetle Philaenus spumarius. The use of microorganisms such as entomopathogenic nematodes and fungi is a well-known biocontrol strategy against agricultural pests. This study aims to evaluate the pathogenicity of two different entomopathogenic fungi (EPF), the commercial Beauveria bassiana strain ATCC74044 (Naturalis®) and Lecanicillium muscarium Ve6 (Mycotal®), and two indigenous strains of EPNs against juveniles of the meadow spittlebug Philaenus spumarius. Monitoring of insect vectors is necessary to prevent the further spread of Xylella fastidiosa in Europe. The rapid spread of Xylella fastidiosa underlines the importance of preventive biocontrol measures against the vector with EPNs and EPF. Key Words: Xylella fastidiosa, insect vector, Philaenus spumarius, entomopathogenic fungi entomopathogenic nematodes

Diversity of plant pollen sources, microbial communities, and phenolic compounds present in bee pollen and bee bread.

The pollination of several crops, as well as wild plants, depends on honeybees. To get the nutrients required for growth and survival, honeybee colonies are dependent on pollen supply. Bee pollen (BP) is partially packed in honeycomb cells and processed into beebread (BB) by microbial metabolism. The composition of pollen is highly variable and is mainly dependent on ecological habitat, geographical origin, honey plants, climatic conditions, and seasonal variations. Although there are important differences between the BP and the BB, little comparative chemical and microbiological data on this topic exists in the literature, particularly for samples with the same origin. In this study, BP and BB pollen samples were collected from two apiaries located in the Campania and Molise regions of Southern Italy. Phenolic profiles were detected via HPLC, while antioxidant activity was determined by ABTS·+ and DPPH· assay. The next-generation sequencing (NGS) based on RNA analysis of 16S (rRNA) and internal transcribed spacer (ITS2) regions were used to investigate the microbial community (bacteria and fungi) and botanical origin of the BP and BB. Chemical analysis showed a higher content of flavonols in BP (rutin, myricetin, quercetin, and kaempferol), while in BB there was a higher content of phenolic acids. The NGS analysis revealed that the microbial communities and pollen sources are dependent on the geographical location of apiaries. In addition, diversity was highlighted between the microbial communities present in the BP and BB samples collected from each apiary.

Exploring Apulia’s Regional Tourism Attractiveness through the Lens of Sustainability: A Machine Learning Approach and Counterfactual Explainability Process

Visitor attraction dynamics lead tourism industry paths. A complex artificial neural network model was built to predict the incoming tourism flow in the Apulia region of Southern Italy as a function of the heterogeneity of the tourism supply available in this area. Open data from the Regional Tourism Observatory were targeted. Information on the distribution of facilities and activities that attract regional tourist flows was collected and grouped by municipality. An artificial neural network model was built with total tourist attendance as the dependent variable and tourist attractions as regressors. The Root Mean Square Error (RMSE) was used to select the optimal model using the lowest value. The final model was run with a hidden layer consisting of three neurons and a decay value of 0.01. A Multi-Objective Counterfactual model (MOC) was then constructed using a randomly selected row of normalized data frame to validate a useful tool in increasing total tourist attendance by 20% over that of the randomly selected municipality. A Garson’s variables importance plot indicated natural landscapes such as beaches, sea caves, and natural parks have a primary role expressed in terms of variable importance in the AI algorithm when used as an innovative methodology for evaluating tourism flows in the Apulia region. A further MOC model built using a randomly selected row of normalized data frame showed convents, libraries, historical buildings, public gardens, and museums as the top five features most modified to improve total attendance in a randomly selected municipality. Use of AI modeling revealed that the implementation of nature-based solutions may speed up the flow of tourism in the Apulia region while also promoting sustainable social development.

Willingness to accept a wind power plant: A survey study in the South of Italy

Public opposition to wind power plants has led to the abandonment of numerous proposed projects. Focusing on a recently proposed project, we test some of the most relevant determinants of community acceptance found in the literature, as well as propose new ones. In 2022, shortly after the public announcement of a new off-shore wind farm installation in a tourist region of Southern Italy, we conducted an in-the-field survey. The survey targeted nearly 1000 individuals at the region's most prominent mass-gathering festival, which attracts both residents and tourists. Our questionnaire is unique in its inclusion of three temporal questions (past, present, future), allowing us to assess how interviewees perceive the seriousness of climate change and how this perception evolves over time. Our findings contradict the NIMBY hypothesis and support the importance of place attachment among both residents and tourists. Additionally, we identify a positive impact of environmental attitudes. Most notably, we highlight the role of climate change perception in the medium term: individuals who express greater concern about climate change in the future compared to the past and present are more likely to accept the project. These findings are validated by another survey that we conducted under similar conditions in 2023.

Techno-Economic Analysis of Clean Hydrogen Production Plants in Sicily: Comparison of Distributed and Centralized Production

This paper presents an assessment of the levelized cost of clean hydrogen produced in Sicily, a region in Southern Italy particularly rich in renewable energy and where nearly 50% of Italy’s refineries are located, making a comparison between on-site production, that is, near the end users who will use the hydrogen, and centralized production, comparing the costs obtained by employing the two types of electrolyzers already commercially available. In the study for centralized production, the scale factor method was applied on the costs of electrolyzers, and the optimal transport modes were considered based on the distance and amount of hydrogen to be transported. The results obtained indicate higher prices for hydrogen produced locally (from about 7 €/kg to 10 €/kg) and lower prices (from 2.66 €/kg to 5.80 €/kg) for hydrogen produced in centralized plants due to economies of scale and higher conversion efficiencies. How-ever, meeting the demand for clean hydrogen at minimal cost requires hydrogen distribution pipelines to transport it from centralized production sites to users, which currently do not exist in Sicily, as well as a significant amount of renewable energy ranging from 1.4 to 1.7 TWh per year to cover only 16% of refineries’ hydrogen needs.

What Italian PICU Healthcare Providers Think About Donation After Cardiocirculatory Determination of Death: A National Survey

ABSTRACTBackgroundItaly presently does not have a pediatric organ donation program after cardiocirculatory determination of death (pDCDD). Before implementing a pDCDD program, many centers globally have conducted studies on the attitudes of pediatric intensive care unit (PICU) staff. This research aims to minimize potential adverse reactions and evaluate the acceptance of the novel donation practice.MethodsWe conducted an electronic and anonymous survey on attitudes toward pDCDD among healthcare professionals (HCPs) working at eight Italian PICUs. The survey had three parts: (I) questions about general demographic data; (II) 18 statements about personal wishes to donate, experience of discussing donation, and knowledge about donation; (III) attitudinal statements regarding two pediatric Maastricht III scenarios of organ donation.ResultsThe response rate was 54.4%, and the majority of respondents were nurses. Of those who responded, 45.3% worked in the Center, 40.8% in the North, and 12.8% in the South of Italy. In total, 93.9% supported pediatric organ and tissue donation, 90.3% supported donation after neurological determination of death (DNDD), 78.2% supported pDCDD, and 69.7% felt comfortable about the idea of participating in pDCDD on Type III patients, with a higher percentage of supportive responses in the Center (77.2%) than in the North (65.1%) and South (54.5%) of Italy (p‐value < 0.004). Concerning scenarios, 79.3% of participants believed that organ retrieval took place in a patient who was already deceased. Overall, 27.3% considered their knowledge about DCDD to be adequate.ConclusionsOur study provides insight into the attitudes and knowledge of PICU staff members regarding pDCDD in Italy. Despite a general lack of knowledge on the subject, respondents showed positive attitudes toward pDCDD and a strong consensus that the Italian legislation protocol for determining death based on cardiocirculatory criteria respects the “dead donor rule.” There were several distinctions among the northern, central, and southern regions of Italy, and in our view, these disparities can be attributed to the varying practices of commemorating the deceased. In order to assess how practice and training influence the attitude of PICU staff members, it would be interesting to repeat the survey after the implementation of a program.

Physical and Economic Water Productivity in Agriculture between Traditional and Water-Saving Irrigation Systems: A Case Study in Southern Italy

Water scarcity is a growing social, economic, and political issue, especially in Southern European countries that are becoming even more arid and where different crops can be cultivated only if irrigation is possible. In this context, strategies to enhance water use efficiency are regarded as critical from both an economic and an environmental standpoint. The present work aims to analyse water use efficiency and productivity of processing tomato in Apulia region of Southern Italy. Specifically, the study examines the potential enhancements in economic and physical water productivity through the simulation of the fully coupled FEST-EWB-SAFY model, a hydrological crop model that estimates the optimal water requirements for irrigation using satellite and ground data. The model’s estimates suggest that plants require significantly less water than that provided by conventional irrigation systems. The simulations also suggest that information technology, when combined with irrigation water-saving techniques, can lead to a reduction in water waste, an increase in water productivity, and lower incidence of water costs. Policy interventions should integrate water efficiency into existing regulatory measures and promote better water usage planning through the adoption of smart delivery systems aimed at supplying water only when necessary and at optimal volumes.

Occurrence, Impact, and Multilocus Sequence Analysis of Alder Yellows Phytoplasma Infecting Common Alder and Italian Alder in Southern Italy.

Alder yellows (ALY) phytoplasma (16SrV-C) is associated with ALY, a disease of several Alnus (alder) species in Europe and A. rubra in North America. In all affected species, the symptoms are similar. However, latent infections are common. ALY phytoplasma includes different strains which may be occasionally transmitted to grapevines leading to some grapevine yellows diseases. In the current study, visual symptom assessment and PCR-based methods using universal and group-specific phytoplasma primers were used to update and extend knowledge on the occurrence, impact, and genetic diversity of ALY phytoplasma in declining and non-symptomatic A. glutinosa and A. cordata trees in the Basilicata and Campania regions of southern Italy. ALY phytoplasma was detected in 80% of alder trees examined. In symptomatic trees, no other cause of disease was observed. More than half of alder trees that tested phytoplasma-positive proved to be latently infected. A considerable genetic variability was observed among the newly recorded ALY phytoplasma strains in southern Italy in almost of the genes examined. These included 16S rRNA, 16S/23S rDNA spacer region, ribosomal protein rpsV (rpl22) and rpsC (rps3), map, imp, and groEL genes. Eleven new genotypes were identified at map gene sequence level. However, the genetic differences observed were not related to plant host species, geographical origin, and symptoms shown by infected alder trees. Also, this study indicates that ALY phytoplasma is more widespread than previously thought.

Assessment of Yield and Quality of Eggplant (Solanum melongena L.) Fruits Improved by Biodegradable Mulching Film in Two Different Regions of Southern Italy

Low-density polyethylene (LDPE) mulching films have an important function in crop cultivation; at the end of their life, however, their removal and disposal become both an economic and environmental problem. One possible alternative to low-density polyethylene (LDPE) mulch is provided by certified soil-biodegradable mulch films, such as those produced by Novamont and commercially available under the trade name MaterBi®. MaterBi is a biodegradable thermoplastic material made with starch and a biodegradable copolyester based on proprietary technology. In this study, we compared two biodegradable MaterBi®-based films (commercial and experimental films) with bare soil and a low-density polyethylene to evaluate their effect on yield and on a number of qualitative characteristics (organoleptic and nutraceutical composition) of eggplant fruits (cv Mirabelle F1) grown in two different regions in Southern Italy (Sicily and Campania). In our study, the use of biodegradable MaterBi® films improved not only yield and production parameters, such as the number and average weight of fruits, but also lipophilic and hydrophilic antioxidant activity and phenolic and ascorbic acid content. For many parameters, responses differed according to the cultivation environment and, in particular, the site’s pedoclimatic conditions. Our results suggest that biodegradable MaterBi®-based mulching films are a potentially valid alternative to traditional LDPEs, providing the production and quality benefits reported above and promoting environmental sustainability, thanks to their positive biodegradable properties.

The Local Regression Approach as a Tool to Improve Place-Based Policies: The Case of Molise (Southern Italy)

The implementation of place-based policies entails the construction of intervention areas (spatially contiguous areas in which the policies are adopted). Many approaches can be adopted for the definition of such areas. This paper reflects on the use of geographically weighted regression (GWR) models as a tool capable of supporting the definition process. The case study concerns Molise, a region in Southern Italy particularly affected by persistent and deep-rooted processes of depopulation. The dependent variable is the average annual rate of population change of municipalities of Molise across the 2011–2019. The independent variables are related to socio-economic profiles of each municipality. The results, contextualised using a broad overview of the Italian case, show that a key variable in the demographic dynamics of the municipalities of Molise is the labour market activity rate of women and that this variable drives a spatial instability that cannot be detected using global approaches and models. This proves the urgent need to expand the use of local thinking for the benefit of both applied demography and society.

Ready-to-use broccoli raab (Brassica rapa L. subsp. sylvestris) quality and volatilome as affected by packaging

Broccoli raab (Brassica rapa L.) is a cruciferous vegetable primarily grown in the southern regions of Italy. Distinguished from the more common broccoli head (B. oleracea), its edible parts consist of sprouts, buds, younger stems, and leaves. Considering the high perishability of broccoli raab, it is necessary to apply postharvest technologies to make it a ready-to-use product. In this paper, active modified atmosphere packaging (aMAP, closed at 3% O2 and 97% N2) was applied, in combination with two types of packaging materials (polypropylene/polyamide microperforated, PP/PA MF, and polypropylene, PP). Shrink-wrap packaging and air-storage were included as control measures. The purpose was to assess the effects of different packaging conditions on enhancing knowledge about volatile compound development and quality aspects of the produce. Both packaging materials resulted in a 10% accumulation of CO2, combined with 1% O2 for PP bags and 10% for PP/PA. Different gas mixtures led to varied sensory and volatile development in minimally processed broccoli raab during storage. PP exhibited the lowest odor score (1.2, well below the marketability limit) and the highest concentrations of off-odor-related volatile compounds (ethanol, acetaldehyde, dimethyldisulfide, dimethyltrisulfide, and methanethiol) while samples packed in PP/PA MF reached an intermediate odor score with superior visual quality evaluation up to 12 days of storage. Both packaging materials reduced weight loss compared to control samples, up to less than 2% and 6%, respectively. In conclusion, active MAP using PP/PA MF bags demonstrated the best suitability in maintaining broccoli raab quality, with a final gaseous composition of 10% O2 and 12% CO2 deemed optimal for storage.

Whole-genome analysis suggesting probiotic potential and safety properties of Pediococcus pentosaceus DSPZPP1, a promising LAB strain isolated from traditional fermented sausages of the Basilicata region (Southern Italy).

Many lactic acid bacteria (LAB) strains are currently gaining attention in the food industry and various biological applications because of their harmless and functional properties. Given the growing consumer demand for safe food, further research into potential probiotic bacteria is beneficial. Therefore, we aimed to characterize Pediococcus pentosaceus DSPZPP1, a LAB strain isolated from traditional fermented sausages from the Basilicata region of Southern Italy. In this study, we analyzed the whole genome of the P. pentosaceus DSPZPP1 strain and performed in silico characterization to evaluate its applicability for probiotics and use in the food industry. The whole-genome assembly and functional annotations revealed many interesting characteristics of the DSPZPP1 strain. Sequencing raw reads were assembled into a draft genome of size 1,891,398 bp, with a G + C content of 37.3%. Functional annotation identified 1930 protein-encoding genes and 58 RNAs including tRNA, tmRNA, and 16S, 23S, and 5S rRNAs. The analysis shows the presence of genes that encode water-soluble B-group vitamins such as biotin, folate, coenzyme A, and riboflavin. Furthermore, the analysis revealed that the DSPZPP1 strain can synthesize class II bacteriocin, penocin A, adding importance to the food industry for bio-enriched food. The DSPZPP1 genome does not show the presence of plasmids, and no genes associated with antimicrobial resistance and virulence were found. In addition, two intact bacteriophages were identified. Importantly, the lowest probability value in pathogenicity analysis indicates that this strain is non-pathogenic to humans. 16 s rRNA-based phylogenetic analysis and comparative analysis based on ANI and Tetra reveal that the DSPZPP1 strain shares the closest evolutionary relationship with P. pentosaceus DSM 20336 and other Pediococcus strains. Analysis of carbohydrate active enzymes (CAZymes) identified glycosyl transferases (GT) as a main class of enzymes followed by glycoside hydrolases (GH). Our study shows several interesting characteristics of the isolated DSPZPP1 strain from fermented Italian sausages, suggesting its potential use as a promising probiotic candidate and making it more appropriate for selection as a future additive in biopreservation.

Analysis of trends in productivity metrics in assessing land degradation: A case study in the Campania region of southern Italy

Land degradation is a critical issue at a global level and its progressive increasing greatly reduces soil ecosystem services. In this context, the 2030 Agenda for Sustainable Development, adopted by all United Nations Member States in 2015, defined the Sustainable Development Goals (SDGs) and indicated some targets of particular interest for a territory to be integrated into short- and medium-term national programs. Target 15.3, which aims to end desertification and restore degraded lands, is currently monitored by indicator 15.3.1, measured as the combination of three sub-indicators (trends in land cover change, land productivity and carbon stocks) as suggested by the United Nations Convention to Combat Desertification (UNCCD), the custodian agency for the SDG indicator. In our opinion, this assessment shows some weakness that are generally caused by a lack of information from direct field observations. The greatest limitation regards land productivity dynamics linked to the NDVI trajectory adopted by the UNCCD methodological approach. For this reason, the paper proposes an alternative approach that consists of using annual maximum NDVI value assessments instead of annual mean values for trajectory calculation. To come to these conclusions, the study addresses a reliability assessment by using remote sensing techniques via the Google Earth Engine (GEE) and analysing the NDVI evolution over time at 450 locations spread around the Campania region (southern Italy). To this end, a customised Graphical User Interface (GUI) was built on the GEE platform and a Google Earth time slider tool was applied to visualize land cover changes which occurred at each location over a period of 18 years (2001–2018). The survey was carried out on MODIS and Landsat 7 collections and showed that the new approach had a better performance than the UNCCD approach (90 % vs 62 % of successful reliability tests, up to 96 % considering results from Landsat images). The application of maximum NDVI values to assess productivity dynamics spatially shows, with regard to UNCCD data, more than double the percentages of degraded and stable lands and a drastic reduction in improved areas within the Campania region. Overall, this innovative approach appears to agree more closely with ground truth and the use of finer resolution data is more suitable for investigating land degradation processes within a regional context.