Refrigeration Storage Research Articles

Recovery of culturable Streptococcus pyogenes from swabs stored at different temperatures.

Improving our understanding of superficial Streptococcus pyogenes (Strep A) carriage and transmission necessitates robust sampling methods. Here, we compared the effect of storing swab samples in fridge (+4°C) and freezer (-20°C) conditions on the recovery of laboratory-cultured S. pyogenes. Streptococcus pyogenes colony-forming units progressively declined at +4°C, but not at -20°C, over 2 weeks. Results demonstrate that freezing is preferable over refrigeration for storage and transport of skin and throat swabs to ensure that culturing accurately reflects the true results of sampling. This is particularly important in remote community research and practice when immediate incubation is not possible or batch processing is most practical, increasing the elapsed time between collection and laboratory analysis. The study suggests that temperature negatively affects S. pyogenes viability and provides a method to further investigate the role of other environmental factors affecting S. pyogenes transmission.

Enhancing grape preservation: The synergistic effect of curry leaf essential oil in buckwheat starch-chitosan composite coatings

A novel edible composite coating composed of buckwheat starch (BS) and chitosan (CH) with varying levels of curry leaf essential oil (CLEO) was synthesized in the present study. Initially, starch was extracted from buckwheat groats, and its proximate analysis revealed a high amylose content (21.91 ± 0.32%), which makes it an excellent base material for coating formation. The composite coatings were created by blending BS and CH with CLEO in different concentrations. The coatings underwent a comprehensive evaluation, including physico-chemical, functional, thermal analyses, and microstructural characterization. Cytotoxicity tests on human cell lines (Caco-2) confirmed that the coatings were non-toxic. Among the various formulations, the coating with 2.0% CLEO (referred to as F5) exhibited the highest antimicrobial activity, maximum antioxidant properties, and the lowest water solubility. Following this, the coatings were applied to green grapes (Vitis vinifera) via dip coating technique. The performance of coatings was assessed by storing the grapes under room temperature (25 ± 1 °C) and refrigeration conditions (4 ± 1 °C). From visual appearance and physio-chemical tests, it was concluded that the F5 coating was potentially active in extending the shelf life of grapes to 12 days at room temperature and to 20 days under refrigeration storage. Overall, the BS-CH-CLEO composite coating emerges as a refined and eco-conscious bioactive packaging solution, elegantly extending the shelf life of grapes while providing both sustainability and cost-efficiency.

Development of inorganic phase change material and cold charging performance analysis based on cold storage refrigerator

Phase change cold storage refrigerators are a core of low-carbon development in cold chain logistics. This study is dedicated to optimizing the performance of phase-change cold storage refrigerators for the refrigerated transport of fruits and vegetables. Firstly, a high-performance inorganic phase change material was developed. By selecting a ratio of 2 wt% Carboxymethyl cellulose and 0.25 wt% Fumed Silica to solve the defects of supercooling and phase separation of this material, Subsequently, an experimental platform was built based on this material to test the charging and cooling characteristics of the phase change module. It was found that the opening of the expansion valve, the compressor’s speed, and the condenser fan’s speed were positively correlated to the charging and cooling efficiencies. Still, the effects of the first two on the system performance coefficient had optimal value points. However, there is an optimum point for the impact of the first two on the system performance coefficient. The optimized system coefficient of performance is increased to 1.62, and the charging time is reduced by 47.6 %, which is a good balance between energy efficiency and transport efficiency. In addition, it was found that the radial thermal resistance of the evaporator tubes in the charging module was more significant than the axial direction, and a new type of finned tube was designed to enhance the heat transfer, which resulted in a further reduction of 29 percent in the charge time.

Study on off-grid performance and economic viability of photovoltaic energy storage refrigeration systems

Study on off-grid performance and economic viability of photovoltaic energy storage refrigeration systems

Designing and testing a heat pump unit for drying, low-temperature processing, and storing of food products

The use of heat pumps makes it possible to reduce energy consumption in drying processes. This study addresses the problem of efficient use of a heat pump for drying, low-temperature processing, and storage of food products. The object of the study is a heat pump unit capable of operating under the drying mode or under the refrigeration processing and storage mode. Studies have been conducted to design an experimental unit, as well as to improve the energy efficiency of the cooling system. The unit includes a single-stage freon compressor, two air condensers, a water-cooled coil heat exchanger-precondenser, an air evaporator, a thermostatic valve, axial fans, and a unit control system. It has been experimentally established that, depending on the temperature level of condensation of the refrigerant (from +20 °C to +50 °C), the increase in specific cooling capacity with the heat exchanger-precondenser turned off is from 82 kJ/kg to 135 kJ/kg, and with it turned on – from 98 kJ/kg to 143 kJ/kg. In this case, the specific work of compression of refrigerant vapors in the compressor with the heat exchanger-precondenser switched off changes from 36 kJ/kg to 18 kJ/kg. And when it is switched on – from 32 kJ/kg to 10 kJ/kg. That is, the reduction in specific energy consumption is from 4 kJ/kg to 8 kJ/kg, which indicates the advisability of including the precondenser in the installation scheme. In the installation, refrigeration treatment and storage of the product can be carried out in the temperature range from –20 °C to +20 °C and drying – from +10 °C to +40 °C. Under such modes, it is possible to obtain high-quality products with long shelf lives. The results could be used to improve the designs and optimize the operation of heat-transfer drying units and their engineering calculations

Effectiveness of Ozone Treatment and Packaging Techniques in Preserving Taiwanese Domestic Beef During Refrigerated Storage.

This study investigates the efficacy of ozone treatment combined with different packaging methods on the preservation of Taiwanese domestically produced beef during refrigerated storage. The preservation of fresh beef is crucial for ensuring food safety and quality; we do not know whether changing the packaging method can mitigate the negative effects of ozone on meat and even enhance its positive impact. Beef samples were treated with ozone and packaged using the vacuum or PVDC-tray methods, then stored at 4 °C for 7 days. The results show that ozone treatment effectively inhibited microbial (total plate count, Salmonella, and Escherichia coli) growth (p < 0.05). Vacuum packaging maintained lower TBARS values (p < 0.05) and metmyoglobin percentages compared to PVDC-tray packaging (p < 0.05). The L* values of all treatments increased over storage time, with significant differences observed between days 0 and 7. Ozone treatment combined with vacuum packaging demonstrated promising results in inhibiting microbial growth and preserving beef quality during refrigerated storage. These findings contribute to enhancing the safety and shelf life of Taiwanese domestically produced beef, potentially benefiting both producers and consumers.

Design, optimization, and performance analysis of a solar-wind powered compression chiller with built-in energy storage system for sustainable cooling in remote areas

This study aims to develop a sustainable cooling solution for refrigeration in remote areas, utilizing solely wind and solar power. Ensuring that the power generated aligns with consumption throughout the day is a critical challenge. The investigation centers on the integration of an energy storage and a compression cooling cycle to attain the desired objective. This system integrates refrigerant storage on both the evaporator and condenser sides. The storage tanks' volume is variable by time and should be adjusted automatically. The sustainability of the cooling capacity depends on the amount of input power and the type of refrigerant. Moreover, additional heat is extracted to produce hot water. The analysis includes the coefficient of performance, exergy efficiency, cooling capacity, total heating capacity, and environmental impacts for six refrigerants R134a, R22, R717, R125, R500, and R407c. The system is configured to offer a cooling capacity of 15 kW while maintaining a temperature difference of 38K. The best performance is achieved using R717 as the refrigerant with a coefficient of performance of 5.26, an exergy efficiency of 36 %, and a payback period of 2.267 in Sabzevar. Furthermore, R717 does not contribute to ozone depletion or global warming.

Enhancing Stability and Investigating Target Attainment of Benzylpenicillin in Outpatient Parenteral Antimicrobial Therapy: Insights from In Vitro and In Vivo Evaluations.

Background/Objective: Narrow-spectrum beta-lactam antibiotics such as benzylpenicillin and flucloxacillin are increasingly used in outpatient parenteral antimicrobial therapy (OPAT) programs to mitigate the adverse effects associated with broad-spectrum antibiotics. These beta-lactams require continuous administration via portable infusion devices during OPAT. However, the use of benzylpenicillin in OPAT requires special consideration because of its limited stability at elevated temperatures. Methods: We tested the benzylpenicillin stability, pH, and degradation of products in elastomeric pumps at different concentrations in saline and in buffered solution containing sodium citrate during a prolonged storage and at high temperatures (seven days at 2-8 °C followed by 24 h at 37 °C). Additionally, drug concentrations during intermittent bolus infusion and during OPAT were determined in five patients. The concentrations and degradation products of benzylpenicillin were measured using liquid chromatography mass spectrometry (LC-MS/MS). Results: Unbuffered benzylpenicillin solutions that were already degraded during refrigerator storage and analyte concentration were not measurable after 8 days. The stability of the buffered solutions was acceptable at all three of the tested concentrations (97.6 ± 1.3%, 96.3 ± 0.8%, and 94.9 ± 1.1% for 10 Mio IU, 20 Mio IU, and 40 Mio IU of benzylpenicillin). The stability was influenced by benzylpenicillin concentration, and several breakdown products were identified. Benzylpenicillin concentrations were measured in five patients during OPAT and ranged from 7.2 to 60 mg/L. Conclusions: Benzylpenicillin buffered with sodium citrate is a safe and convenient option for use in continuous infusions during OPAT and should be favored over broad-spectrum antibiotics. Therapeutic drug monitoring data indicate sufficient to high plasma levels when patients received benzylpenicillin as continuous infusions.

Fatty acid composition, lipid profile and oxidative stability of meat of broiler chickens fed diet containing bird eye pepper of varying inclusion level and sieve size.

Nutritional modifications to improve meat quality is targeted by farmers. Bird eye pepper (BEP) contains bio-compounds of physiological significance. The potency of BEP of varying inclusion level and sieve size on meat quality [fatty acid (FA), lipid profile and oxidative stability] of broiler chickens was investigated. A total of 246 birds fed diet-containing BEP [inclusion level (0, 0.15 and 0.3%), sieve size (0.05 and 0.1mm)] were randomized to six treatments replicated 4 times in a 2 by 3 factorial layout. After feeding (31 days), forty-eight birds (two per replicate) were slaughtered and breast muscles harvested. Meat lipid profile and 2-thiobarbituric acid reactive substance (TBARs) were determined on day (d) 0, while TBARs was further assessed on d 3 and 5, but FA on d 10 of refrigeration storage. BEP diet (0.15%) increased (p < 0.05) total monounsaturated FA (MUFA), unsaturated FA (UFA) and n-3 FA, while 0.05mm BEP lowered (p < 0.05) meat index of thrombogenicity (TI) but increased meat hypocholesteromic: hypercholesteromic (HH) value. Dietary 0.15% (0.05mm) BEP yielded low (p < 0.05) SFA but high MUFA: SFA, UFA: SFA and NVI, while 0.15% (0.1mm) BEP diet resulted in high total MUFA and higher (p < 0.05) UFA, n-3 and n-3: n-6 FA. Control, 0.15% and 0.05mm BEP diets reduced (p < 0.05) meat cholesterol value. This study has shown that 0.15% (0.05mm) BEP diet had no deleterious effect on the growth of broiler chickens but improved the NVI, IA, TI, HH, TBARs and cholesterol of the meat - a significance to health-conscious consumers.

Performance analysis and multi-objective optimization for an integrated air separation, power generation, refrigeration and ice thermal storage system based on the LNG cold energy utilization

Against the backdrop of escalating resource depletion and the urgent quest for alternative sources, liquefied natural gas (LNG) is increasingly gaining prominence as a sustainable solution, particularly in refrigeration applications. However, its underutilization results in wasted resources. To efficiently harness the released cold energy from LNG gasification, this study proposes an integrated system comprising air separation, power generation, refrigeration, and ice thermal storage. The system undergoes optimization using the non-dominated sorting genetic algorithm II (NSGA-II) to determine the optimal operating parameters. The optimized system is comprehensively analyzed from energy, exergy, economic, and environmental perspectives. Results show that the system, with a 70t/h LNG capacity, achieves an energy efficiency of 42.52% and an exergy efficiency of 48.09%. Economically, the system incurs a cost of approximately 0.0711 $/kWh and can mitigate over 1.4504*107kg of CO2 emissions. Compared to traditional LNG utilization systems, the integrated system demonstrates a 22.32% improvement in energy efficiency, a 7.69% enhancement in exergy efficiency, and a cost reduction of 0.0049 $/kWh. In summary, this technologically advanced and economically viable system offers a significant alternative to optimize LNG cold energy utilization.

Fabrication of stable hydrogel microspheres with hydrophobic shell using water-in-water (W/W) Pickering emulsion template

Hydrogel microspheres are promising for food applications. The water-in-oil (W/O) emulsion template is commonly used to prepare the hydrogel microspheres. However, this method often involves synthetic surfactants and toxic organic solvents to remove the oil phase. The swelling problem of the resultant microspheres is another obstacle. In this study the water-in-water (W/W) emulsion template of gelatin-in-dextran was used to prepare the hydrogel microspheres, without the addition of surfactants, toxic reagents and high energy input. To prevent swelling, zein particles modified with alginate were served as a hydrophobic shell of the gelatin core. The formation evolution of these core–shell microspheres and its relevant factors including phase behavior of the immiscible gelatin/dextran, the binding of zein/alginate and the addition of the modified zein particles were investigated. It was found that small amount of alginate could induce zein particles to absorb on the gelatin surface, whereas the excess led to absorption competition. When using 0.6 wt% particles with zein/alginate ratio of 1:0.5, the ideal core–shell microspheres which were fully covered by the particles and with a self-supporting architecture in uniform size (18.8 ± 0.1 μm) and spherical shape were obtained. These microspheres showed remarkable stability under the conditions of heat treatment, varied pHs and salt concentrations and long-term storage either in refrigerator or room environment. Their sizes were easily manipulated by adjusting the concentration and molar mass of the biopolymers. It is believed that the desired stability and tunable sizes of these edible core–shell microspheres could contribute the development of their applications in foods.

Design of innovative phase-change cold storage refrigerator and simulation analysis of discharging progress

At present, the field of cold chain logistics experiences significant growth, with refrigerator playing a pivotal role in cold chain transportation. An innovative compartmentalized phase-change cold storage refrigerator has been proposed in this study, designed to address the increasing demand for efficient and energy-saving solutions in the cold chain transportation industry. A conceptual module prototype is designed and built in this study, with an analysis of the system's charging and discharging processes under varying conditions. The cold storage refrigerator exhibits favorable matching characteristics, the charging process requires approximately 270 min, and the cooling effect sustains for about 23 h. A three-dimensional simulation model of the cold storage refrigerator is developed, enabling a comprehensive evaluation of the cooling effect and further optimization of the cold storage plate layout. The results indicate that the layout of the three-sided type cold plate significantly reduces the pre-cooling time. It is determined through simulation calculations in this study that setting the loading capacity to 50 % can strike a balance between efficient cooling and transportation efficiency. Additionally, under high-temperature conditions, the cold storage refrigerator maintains a low temperature for up to 10 h at a filling rate of 50 %.

Investigation of Salmonella enteritidis Growth under Varying Temperature Conditions in Liquid Whole Egg: Proposals for Smart Management Technology for Safe Refrigerated Storage

This study investigates the growth characteristics of Salmonella enteritidis (S. enteritidis) in liquid whole egg under both isothermal and non-isothermal storage conditions to understand the risks associated with inadequate temperature management in the egg industry. Using controlled laboratory simulations, liquid whole egg samples inoculated with S. enteritidis were stored under various isothermal (5, 15, 25, 35, and 45 °C) and non-isothermal conditions (5–10, 15–20, 25–30, 35–40, and 45–50 °C). The growth behavior of the S. enteritidis was analyzed using a two-step predictive modeling approach. First, growth kinetic parameters were estimated using a primary model, and then the effects of temperature on the estimated specific growth rate and lag time were described using a secondary model. Independent growth data under both isothermal and non-isothermal conditions were used to evaluate the models. The results showed that S. enteritidis exhibits different growth characteristics depending on temperature conditions, emphasizing the need for strict temperature control to prevent foodborne illnesses. To address this, a predictive growth model tailored for non-isothermal conditions was developed and validated using experimental data, demonstrating its reliability in predicting S. enteritidis behavior under dynamic temperature scenarios. Additionally, temperature management technologies were proposed and tested to improve food safety during refrigerated storage. This study provides a scientific basis for improving food safety protocols in the egg industry, thereby protecting public health and maintaining consumer confidence amid temperature fluctuations.

Lactiplantibacillus plantarum 299V fermented in microcapsules shows enhanced stability and could improve the microbial quality and safety of oysters through bioaccumulation.

In this study, microcapsules of Lactiplantibacillus plantarum 299V were prepared using an emulsification/internal gelation technique. Loads of the probiotics were condensed to 9.86±0.13logCFU/g after 24h fermentation of the microcapsules. Physical characterization revealed that L. plantarum 299V cells were uniformly distributed within the core of the microcapsules, with a mean diameter of 109.81±0.39µm and a span value of 0.36±0.00, which were comparable to those of the unfermented microcapsules (p>0.05). The viability of L. plantarum 299V in the fermented microcapsules was 2.08±0.15log higher than that of free cells at the end of 5h simulated gastrointestinal digestion (p<0.05). Oysters were able to accumulate the fermented microcapsules through filter feeding, resulting in a load of probiotics exceeding 6.00logCFU/g. The presence of L. plantarum 299V-carrying microcapsules in oyster tissues significantly suppressed spoilage-causing bacteria during 11 days refrigeration storage, suggested by the tested parameters, including total psychrotrophic bacteria, H2S-producing bacteria, and Pseudomonas spp. (p<0.05). Pathogenic bacteria, including Vibrio parahaemolyticus and Salmonella enterica artificially introduced into oysters, were also significantly suppressed by over 1.00-log within 4 days compared to control samples (p<0.05). In summary, oysters bioaccumulated with fermented L. plantarum 299V-carrying microcapsules, justified a novel probiotic-carrying product to exsert the health-promoting effect of probiotics. This solution could also enhance the microbial quality and safety of oysters during storage.

Modeling Behavior of Salmonella spp. and Listeria monocytogenes in Raw and Processed Vegetables.

Given the persistent occurrence of foodborne illnesses linked to both raw and processed vegetables, understanding microbial behavior in these foods under distribution conditions is crucial. This study aimed to develop predictive growth models for Salmonella spp. and Listeria monocytogenes in raw (mung bean sprouts, onion, and cabbage) and processed vegetables (shredded cabbage salad, cabbage and onion juices) at various temperatures, ranging from 4 to 36 °C. Growth models were constructed and validated using isolated strains of Salmonella spp. (S. Bareilly, S. Enteritidis, S. Typhimurium) and L. monocytogenes (serotypes 1/2a and 1/2b) from diverse food sources. The minimum growth temperatures for Salmonella varied among different vegetable matrices: 8 °C for mung bean sprouts, 9 °C for both onion and cabbage, and 10 °C for ready-to-eat (RTE) shredded cabbage salad. Both pathogens grew in cabbage juice at temperatures above 17 °C, while neither demonstrated growth in onion juice, even at 36 °C. Notably, Salmonella spp. exhibited faster growth than L. monocytogenes in all tested samples. At 8 °C, the lag time (LT) and specific growth rate (SGR) for Salmonella spp. in mung bean sprouts were approximately tenfold longer and threefold slower, respectively, compared to those at 10 °C. A decrease in refrigerator storage temperature by 1 or 2 degrees significantly prevented the growth of Salmonella in raw vegetables. These findings offer valuable insights into assessing the risk of foodborne illness associated with the consumption of raw and processed vegetables and inform management strategies in mitigating these risks.

Effects of Preanalytical Sample Collection and Handling on Comprehensive Metabolite Measurements in Human Urine Biospecimens.

Epidemiology studies evaluate associations between the metabolome and disease risk. Urine is a common biospecimen used for such studies due to its wide availability and non-invasive collection. Evaluating the robustness of urinary metabolomic profiles under varying preanalytical conditions is thus of interest. Here we evaluate the impact of sample handling conditions on urine metabolome profiles relative to the gold standard condition (no preservative, no refrigeration storage, single freeze thaw). Conditions tested included the use of borate or chlorhexidine preservatives, various storage and freeze/thaw cycles. We demonstrate that sample handling conditions impact metabolite levels, with borate showing the largest impact with 125 of 1048 altered metabolites (adjusted P<0.05). When simulating a case-control study with expected inconsistencies in sample handling, we predicted the occurrence of false positive altered metabolites to be low (< 11). Predicted false positives increased substantially (≥63) when cases were simulated to undergo alternate handling. Finally, we demonstrate that sample handling impacts on the urinary metabolome were markedly smaller than those in serum. While changes in urine metabolites incurred by sample handling are generally small, we recommend implementing consistent handling conditions and evaluating robustness of metabolite measurements for those showing significant associations with disease outcomes.

Experimental research on chemisorption energy storage performance for industrial waste heat recovery and conversion

This study investigates a solid chemisorption energy storage system utilizing a multi-component chloride salt composite adsorbent with a mass ratio of NH4Cl, CaCl2, MnCl2, and expanded natural graphite treated with sulfuric acid (ENG-TSA). The system features a shell-and-tube heat exchanger and analyzes refrigeration and heat storage performance under varying industrial low-temperature waste heat (60–110 °C) conditions, with condensation and evaporation temperatures of 0–25 °C and discharging temperatures of 40–60 °C. The results indicate that the multi-salt composite adsorbent reduces hysteresis between adsorption and desorption stages. The highest coefficient of performance (COP) and specific cooling power (SCP) were 0.461 and 328 W/kg, respectively, at an evaporation temperature of 25 °C and a charging temperature of 110 °C. Excessively long cycle times negatively affect system efficiency, with optimal performance achieved at a 40-min cycle. The findings highlight the system's effectiveness for industrial low-grade waste heat recovery, providing a solid foundation for future reactor design and material selection.

Parametric Based Techno‐Economic Evaluation for a Solar Thermal‐PV Integrated Multi‐Commodity Storage Facility

ABSTRACTPostharvest losses and spoilage of agricultural products are a major problem for tropical countries, and it is even more challenging for countries encountering fluctuating power shortages, such as Pakistan. Therefore, this study focused on the energy and economic analysis of cold storage to store three products (potatoes, pomegranates, and potatoes) according to the season and storage span throughout the year. The cooling load of the cold store was supported by a LiBr‐H2O vapor absorption and vapor compression refrigeration system to maintain the desired temperature for each product during cold storage. A solar thermal PV system is installed to operate cold storage refrigeration systems. Cold storage performance was analyzed by developing thermal models of integrated systems using the ambient conditions of Lahore, Pakistan. A parametric study was also conducted to analyze the impact of various working parameters on integrated system performance, and it was found that the maximum peak cooling load of 91 kW inside cold storage is attributed to pomegranates owing to high ambient conditions during its loading month. The product loading rate significantly affects the cooling load of cold storage and varies directly with it, as observed for an increase in the product loading rate from 0 to 50 000 kg/day cooling load also increases from 34 to 87 kW. To meet the thermal demand of the generator of the vapor absorption system, parabolic troughs were installed to operate cold storage, and it was found that a minimum of four PTC were needed to support the peak cooling load at the maximum product loading rate and minimum DNI value. To meet the electrical demand of cold storage electrical equipment and the compressor of the vapor compression system, solar photovoltaic panels were installed, and it was found that a minimum of 618 panels was required at a minimum tilted radiation value. To validate the viability of proposed design system economic analysis was also conducted which revealed a payback period of 12 years for Kinnow and potatoes and 16 years for pomegranates.

Impact of control strategies on energy consumption in cold storage facilities

The fast development of global cold chains has led to a rapid increase in cold storage facilities capacity, in which consumes substantial amounts of energy. However, limited research has been conducted on the energy consumed by cold storage facilities, and the time and economic costs of obtaining long-term measurements of annual energy consumption of cold storage facilities are high. Therefore, in this study, short-term field tests and simulations were combined to establish a transient thermal network model for cold storage, using a specific cold storage case study for validation. The model considers the structure of the hall, which strongly impacts the cooling load. The refrigeration system performance was analyzed on the measured data. Literature and field research have shown temperature range control to be the baseline control strategy used in cold storage refrigeration systems, which ensures that the cold storage temperature remains within the set temperature range at all times. In addition, some researchers have proposed a control strategy based on time-of-use tariff that provides cooling almost throughout the valley period. These two control strategies were applied to frozen and chilled rooms, separately; the results indicated that for both frozen and chilled rooms, the latter control strategy consumes slightly less energy than the former strategy. However, the electricity bill for the latter control strategy are 0.35 and 0.5 times lower than those for the former strategy for frozen rooms and chilled rooms, respectively.

Physiological and Technological Properties of Probiotic Lacticaseibacillus rhamnosus GG Encapsulated with Alginate-Chitosan Mixture and Its Incorporation in Whole Milk.

This study developed and evaluated chitosan-sodium alginate capsules containing the probiotic Lacticaseibacillus rhamnosus GG using extrusion and emulsification techniques. The encapsulated L. rhamnosus GG cells were also evaluated for technological and probiotic-related physiological functionalities, as well as when incorporated in UHT and powdered milk. Extrusion (86.01 ± 1.26%) and emulsification (74.43 ± 1.41%) encapsulation techniques showed high encapsulation efficiency and high survival rates of L. rhamnosus GG during 28days of refrigeration and room temperature storage, especially emulsification capsules (> 81%). The encapsulated L. rhamnosus GG cells showed high survival rates during exposure to simulated gastrointestinal conditions (72.65 ± 1.09-114.15 ± 0.44%). L. rhamnosus GG encapsulated by extrusion and emulsification performed satisfactorily in probiotic-related physiological (pH and bile salts tolerance) and technological properties (positive proteolytic activity, diacetyl and exopolysaccharides production, high NaCl tolerance (> 91%), besides having high heat tolerance (> 76%)). L. rhamnosus GG in extrusion and emulsification capsules had high survival rates (> 89%) and did not significantly affect physicochemical parameters in Ultra-High Temperature (UHT) and powdered milk during storage. The results demonstrate that L. rhamnosus GG can be successfully encapsulated with alginate-chitosan as a protective material through extrusion and emulsification techniques. UHT and powdered milk could serve as appropriate delivery systems to increase the intake of this encapsulated probiotic by consumers.