Range Of Reference Values Research Articles

Normal values and reference ranges for quantitative assessment of myocardial first-pass perfusion by spectral computed tomography. From the Cadami-Spectral study

Abstract Background Spectral computed tomography (SCT) can generate iodine concentration maps according to the tissue iodine uptake, serving as a surrogate marker of organ perfusion. Thus, assessment of the iodine distribution maps derived from SCT allows to detect deviations in normal myocardial perfusion. As of present, reference ranges for the iodine concentration in each myocardial segment of the left ventricle (LV) derived from the first-pass perfusion have not been published. Purpose To provide normal values of first pass perfusion in individuals without coronary artery disease (CAD). Additionally, we explored whether these values could vary according to gender, contrast dosing or the cardiac cycle phase assessed. Methods From October 2021 to January 2024, we retrospectively identified patients who underwent a CT Cardiac Angiography (CTCA) and had no evidence of CAD or structural heart disease. Subjects were scanned using a dual-layer SCT, following a standard local protocol which included a weight-based contrast medium dosing of 0.8 mL/Kg. Iodine density maps were reconstructed using the MCA filter at a phase of 78%. An additional sample of 18 subjects who received a dosing of contrast of 1 mL/Kg and 16 cases scanned using a systolic acquisition and reconstructed at phase of 35% were also included. Spectral reconstructions were combined with tissue segmentation to obtain the spatial distribution of iodine along the myocardium. Mean, median and standard deviations values of density of iodine (mg/ml) were quantified for each segment following the AHA-17 segment model. Results Perfusion data from 80 woman (median age: 60 [IQR: 54-65.6] years) and 47 males (median age: 47 [IQR: 43 – 54] years) were analysed. Despite receiving higher dose of contrast (67.9 vs 59.8 mL; p < 0.05), male subjects exhibited lower iodine density values in the myocardium compared to females (median concentration per segment 1.58 vs 2.01 mg/dL, p<0.001; figure 1). Notably, the volume of the LV myocardium was higher in male than in women (143 mL vs 92 mL, p<0.001). These differences could be partially explained in a mixed model considering the ratio contrast dose/myocardium volume (b = 1.500, p < 0.001). Higher contrast dosing (1 mL/Kg vs 0.8 mL/kg) correlated with higher iodine density values in the myocardium (2.03 vs 1.83 mg/dL, respectively; p = 0.023). No differences were noted when comparing reconstructions at systole vs diastole (1.86 vs 1.80 mg/dL, respectively; p = 0.484). Reference range values of each myocardial segment are listed in table 1. Conclusions Significant gender discrepancies are detected in the analysis of first-pass myocardial perfusion. These variations can be partially explained by differences in the LV myocardium volume. Our findings provide applicable reference ranges of the iodine concentration in myocardium at first pass perfusion in controls without coronary or structural heart disease.

MPC Optimization Algorithm and Strategy for HVAC System under Smart City Construction

As a giant in energy consumption, buildings urgently need to optimize control strategies for the main energy consuming equipment inside buildings. It is of great significance to design advanced control algorithms to improve the efficiency of the main energy consuming equipment in buildings, namely air conditioning, in the current energy shortage. The model predictive control algorithms and strategies were used in this study to control the HVAC system to improve the energy utilization of the city. Then linear matrix inequality with robust model predictive feedback controller was used to optimize and get the model predictive control optimization algorithm. The research results showed that, under the influence of different factors, the three regions controlled by the model predictive control optimization algorithm showed a little overshooting in the initial state. But it was quickly corrected after adjustment. Meanwhile, the average tracking error of temperature and humidity in each region was 0.139◦ C and 0.13g/kg dry air, respectively. The average predicted mean vote was 0.32. In actual office buildings, the proposed algorithm controlled the temperature within the reference value range of 0.1◦ C throughout the entire process. The total electricity consumption and electricity price costs were reduced by 12.11% and 22.54%, respectively. In summary, the proposed method has good performance for HVAC system application, which can effectively realize energy saving and emission reduction and improve human comfort. This method makes important contributions to promote the construction of smart cities and the development of green buildings.

Importance of ferritin research in clinical practice

Serum ferritin (SF) is typically present in serum at concentrations directly related to iron (Fe) storage and is therefore traditionally used as an indicator of Fe levels in body tissues. Reducing its level is the “gold standard” for diagnosing widespread iron deficiency conditions. No less significant is hyperferritinemia — a nonspecific syndrome that occurs when Fe reserves are overloaded, a number of immunoinflammatory, infectious, oncological diseases, liver diseases, etc. In many pathological conditions, the level of SF determines the severity and prognosis of the disease. Ferritin concentrations greater than 1000 ng/mL, regardless of cause, have been shown to be associated with higher mortality. The reason for the increase in the level of SF in liver pathologies (cancer, hepatitis, cirrhosis) is associated with the process of its release from hepatocytes during their destruction. On the other hand, excessive synthesis and/or cellular secretion of ferritin occurs under the influence of various stimuli (cytokines, oxidative stress, hypoxia, oncogenes and growth factors). The interpretation of elevated ferritin values goes far beyond the role of an indicator of replenishment of Fe stores in tissues. Only 10% of cases of hyperferritinemia are associated with iron overload; in most patients, it is defined as the result of the acute phase and a reactive increase in ferritin levels against the background of any disease. The variety of symptoms of iron deficiency syndromes and hyperferritinemia is due to the involvement of many organs and systems, which requires a thorough examination (study of complaints, anamnesis, family and concomitant diseases, as well as the necessary laboratory and instrumental studies) to search for possible causes. Systemic Fe homeostasis must be closely monitored on a regular basis. It is required to comply with the conditions for blood sampling for SF. Reference values for SF concentrations vary depending on the analytical methods used and the population studied. Age and gender play an important role. Given the range of reference values, it is important for a particular patient to focus on the initial level of his ferritin, determined against the background of health and well-being during clinical observation.

Normal Value of Thrombocytes Indices in Indonesian Adults: Focus on Gender and Ages

BACKGROUND: Monitoring platelet count and related indices is crucial for managing hematological disorders. No studies are reporting normal platelet indices in the adult Indonesian population. Therefore, this study was conducted to establish normal reference values for platelet count and indices in a healthy population in Indonesia and investigate their association with platelet count and each parameter.METHODS: This cross-sectional study included healthy adults from both sexes who underwent hematological testing in our laboratory. Two mL of venous blood was drawn and analyzed using an automated machine ADVIA 2120 to identify platelet count, mean platelet volume (MPV), platelet distribution width (PDW), plateletcrit (PCT), mean platelet component (MPC), platelet component distribution width (PCDW), mean platelet mass (MPM), platelet mass distribution width (PMDW), and large platelet count.RESULTS: This study included 1037 subjects with median of 27 (18–58) years old. Normal reference range values for platelet indices in adult Indonesians was established, which was significantly different between males and females (p<0.05) in following parameters: platelet count (191.77–400.37 vs. 203.00–433.00 ×109/L), MPV (7.30–9.91 vs. 7.20–10.00 fL), PDW (38.09–59.83 vs. 37.10–58.50%), PCT (0.17–0.33 vs. 0.17–0.35%), PMDW (0.68–1.06 vs. 0.67–1.04 pg) for males vs. females, respectively. However, other parameters such as MPC, PCDW, MPM, and large platelet, did not show any significant differences.CONCLUSION: The reference intervals of platelet indices in the adult Indonesian population were different from previously established reference values, indicating the importance of dedicated reference interval determination with gender consideration.KEYWORDS: thrombocyte indices, platelet count, hematology reference values, blood platelet analysis

Evaluation of Hormonal and Biochemical Profile of Ladakhi Cow during Estrous Cycle: The Future Perspective of Reproductive Management of Ladakhi Cow

The Ladakhi cow is an indigenous breed from Ladakh, India, and has a unique genetic makeup to adapt to high-altitude hypobaric stress. Furthermore, little study is available on examining the Ladakhi cow’s blood biochemicals and reproductive hormonal status during various stages of the estrus cycle. Hence, the current study was conducted on eight reproductively mature Ladakhi cows at the experimental dairy cattle unit in Leh-Ladakh, India, to determine the changes in serum biochemical and hormonal levels during the estrus cycle. The estrus behaviour was initially observed before the blood sampling on days 0 (the day the cow exhibited heat symptoms), 2nd, 8th, 17th, and 21st days. The serum was isolated for biochemical and hormonal profiling as per standard procedures using commercially available kits. The results revealed that serum biochemicals, viz. Ca, Fe, ALT, AST, ALP, TG, TP, Albumin, Glucose, UA, Creatinine, and Urea levels were within the normal range of cows at high altitudes. However, reproductive hormones, e.g. GnRH, FSH, LH, progesterone, estrogen, PGF2α, IGF-1, and Insulin varied from the reference level at different stages of the estrus cycle. This study’s findings have provided new insights into the serum biochemical and hormonal profiling of Ladakhi cows during the estrus cycle and this information may help to develop a reference range and baseline value for the serum biochemicals and hormonal levels of Ladakhi cows for other breeding programs and health monitoring.

Deteksi Tingkat Kematangan Buah Tomat Dengan Transformasi Ruang Warna HSI

Among the vegetables most commonly consumed by people around the world are tomatoes. One of the potential vegetable commodities to be developed is tomato plants. This plant can thrive in rice fields, dry land, and highlands. Use of Technology Digital images are images that can be processed by computers directly. A matrix with M columns and N rows can be used to describe a digital image. The smallest element in an image is called a pixel or image element, and is the intersection between columns and rows. image processing is the process of processing an image numerically; in this case, each pixel or point in the image is treated. One method of image processing is to use computer software to process each pixel in the image. It is easier for object recognition applications in image processing to identify objects based on differences in hue values when the hue values of objects are limited to a certain value. The color space system that mimics the capabilities of the human eye is called the HSI color space model. HSI incorporates the grayscale or color components of an image. The test image of Tomato fruit with a value of H = 32 S = 0.675 I = 83 can be considered ripe, according to the range of fruit reference values that have been established through the use of the HSI method.

Instrumentation for Monitoring and Early Warning of Tidal Flood Using ESP32S2 Microcontroller and A01NYUB Ultrasonic Sensor in Tambakrejo, Semarang

Abstract A tidal flood early warning system is crucial to minimize the impact and loss in tidal flood-prone areas. This research proposes an instrumentation and information system for tidal flood early warning monitoring by optimizing tidal data in the local area. The efficiency of the instrumentation depends on the accuracy of the sensors installed. This research presents the testing and application of The A01NYUB ultrasonic sensor with The ESP32S2 microcontroller for real-time technology-based water level monitoring and The Internet of Things concept. The collected sensor data is analyzed with an algorithm that has also been proposed in this research. The results show that the ultrasonic sensors can provide a reliable and adequate tide monitoring scheme with an accuracy of RMSE value of 0.08943 and bias value of – 2×10−16. The developed system provides early warning when the sea level reaches 260-280 cm with the MSL datum reference value range of -20 cm to 40 cm. The system has been tested at the Fisherman Boat Harbor, Tambakrejo, Semarang, Central Java, Indonesia. The test has shown the system’s accuracy and has proven that the developed system will help minimize the impact of the tidal flood disaster.

Sonographic biometry of apparently normal parotid gland among adults in Asaba, South-South Nigeria

Background: Detecting subtle changes in the parotid gland size can be difficult when there are no established reference values in a specific area. This can affect patient management and delay early detection of diseases. Therefore, the objective of this study was to describe the size of the parotid gland of adult Nigerian people.Methods: This cross-sectional study included apparently healthy adults. The parotid glands were scanned in various sections using a 7.5 MHz probe after applying ultrasound gel. Each dimension was measured twice, and the mean value was calculat-ed. The 95% reference rages (mean ± 1.96 standard deviation) were described for various dimensions of the parotid glands.Results: Four hundred healthy adults participated in the survey. Their mean (standard deviation) age was 38.6 (14.6) years. The mean (95% reference range) of both parotid lengths was 4.7 (4.1 – 5.3) cm. The right and left parotid transverse sizes were 3.8 (3.4 – 4.2) and 3.7 (2.9 – 4.5) respectively. The right and left parotid depths lateral to the mandible were 0.8 (0.4 – 1.2) and 0.9 (0.3 – 1.5), parotid depths dorsal to the mandible were 2.3 (1.7 – 2.9) and 2.2 (1.8 – 2.6), and parotid volume were 20.3 (13.3 – 27.1) and 19.8 (14.3 – 25.3) cm3. No significant difference was seen between the right and left parotid glands in men and women.Conclusion: This study delineates the mean and reference range values of parotid glands among healthy adults of Nigeria which can be used for clinical decision making.

Normative contact mechanics of the ankle Joint: Quantitative assessment utilizing bilateral weightbearing CT

Alterations in ankle’s articular contact mechanics serve as one of the fundamental causes of significant pathology. Nevertheless, computationally intensive algorithms and lack of bilateral weightbearing imaging have rendered it difficult to investigate the normative articular contact stress and side-to-side differences. The aims of our study were two-fold: 1) to determine and quantify the presence of side-to-side contact differences in healthy ankles and 2) to establish normative ranges for articular ankle contact parameters. In this retrospective comparative study, 50 subjects with healthy ankles on bilateral weight-bearing CT were confirmed eligible. Segmentation into 3D bony models was performed semi-automatically, and individualized cartilage layers were modelled based on a previously validated methodology. Contact mechanics were evaluated by using the mean and maximum contact stress of the tibiotalar articulation. Absolute and percentage reference range values were determined for the side-to-side difference. Amongst a cohort of individuals devoid of ankle pathology, mean side-to-side variation in these measurements was < 12 %, while respective differences of > 17 % talar peak stress and > 31 % talar mean stress indicate abnormality. No significant differences were found between laterality in any of the evaluated contact parameters. Understanding these values may promote a more accurate assessment of ankle joint biomechanics when distinguishing acceptable versus pathological contact mechanics in clinical practice.

Normal Clinical Laboratory Ranges by Age and Sex, and Impact on Study Screening Outcomes in Rural Mali.

The interpretation of a laboratory test result requires an appropriate reference range established in healthy subjects, and normal ranges may vary by factors such as geographic region, sex, and age. We examined hematological and clinical chemistry parameters in healthy residents at two rural vaccine trial sites: Bancoumana and Doneguebougou in Mali, West Africa. During screening of clinical studies in 2018 and 2019, peripheral blood samples from 1,192 apparently healthy individuals age 6 months to 82 years were analyzed at a laboratory accredited by the College of American Pathologists for a complete blood count, and creatinine and/or alanine aminotransferase levels. Based on manufacturers' reference range values, which are currently used in Malian clinical laboratories, abnormal values were common in this healthy population. In fact, 30.4% of adult participants had abnormal neutrophil levels and 19.8% had abnormal hemoglobin levels. Differences by sex were observed in those who were older, but not in those younger than 10 years, for several parameters, including hemoglobin, platelet, and absolute neutrophil counts in hematology, and creatinine in biochemistry. The site-specific reference intervals we report can be used in malaria vaccine clinical trials and other interventional studies, as well as in routine clinical care, to identify abnormalities in hematological and biochemical parameters among healthy Malian trial participants.

ZAWARTOŚĆ SELENU W GONADACH SAMCÓW I SAMIC DZIKA EUROAZJATYCKIEGO (SUS SCROFA) Z WOJEWÓDZTWA MAZOWIECKIEGO

Selenium is counted among the trace elements necessary to maintain metabolic processes occurring in the animal body, including reproductive processes. The aim of the study was to assess selenium content in testes and ovaries of wild boars (Sus scrofa) in an attempt to establish a range of reference values for individuals of this species. Selenium concentrations in tissues tested were determined using spectrofluorometric method after wet mineralization in HNO3 and HClO4 mixture. Based on the results, it was found that male wild boars accumulated significantly higher concentrations of Se than females. The average content of this element in the testes of wild boars was 0.32 mg · kg−1 while in the ovaries it was at the level of 0.14 mg · kg−1 wet weight. In addition, the gonads of males were characterized by a higher variability of the analyzed parameter in relation to samples obtained from female wild boars. The coefficient of variation in their case was almost 40.9, while the value of the coefficient of variation for selenium content in the parenchymal layer of the ovaries was 24.9. On the basis of statistical analysis, highly significant differences were found at p ≤ 0.01 according to the sex of the individuals studied. Despite the fact that samples were taken from individuals living in areas with selenium deficiencies due to the biogeochemical background, it should be considered that the supply of Se is optimal, which is evident in the population growth dynamics of individuals of this species in the study area.

Quantitative Evaluation of the Lumbar Multifidus Muscle by Shear Wave Dispersion in Healthy Adults: A Preliminary Study.

To investigate the application value of shear wave dispersion (SWD) in healthy adults with the lumbar multifidus muscle (LMM), to determine the range of normal reference values, and to analyze the influences of factors on the parameter. Ninety-five healthy volunteers participated in the study, from whom 2-dimensional, shear wave elastography (SWE), and SWD images of the bilateral LMM were acquired in three positions (prone, standing, and anterior flexion). Subcutaneous fat thickness (SFH), SWE velocity, and SWD slope were measured accordingly for analyses. The mean SWD slope of the bilateral LMM in the prone position was as follows: left: 14.8 ± 3.1 (m/second)/kHz (female) and 13.0 ± 2.5 (m/second)/kHz (male); right: 14.8 ± 3.7 (m/second)/kHz (female) and 14.2 ± 3.4 (m/second)/kHz (male). In the prone position, there was a weak negative correlation between the bilateral LMM SWD slope of activity level 2 and level 1 (β = -1.5 (2 versus 1, left), -1.9 (2 versus 1, right), all P < .05), and between the left SWD slope of activity level 3 and level 1 (β = -2.3 [3 versus 1, left], P < .05). The correlation between SWE velocity and SWD slope value changed with the position: there was a weak positive correlation in the prone position (r = 0.3 [left], 0.37 [right], both P < .05), and a moderate positive correlation in the standing and anterior flexed positions (r = 0.49-0.74, both P < .001). SFH was moderately negatively correlated with bilateral SWD slope values in the anterior flexion (left: r = -0.4, P = .01; right: r = -0.7, P < .01). SWD imaging can be used as an adjunct tool to aid in the assessment of viscosity in LMM. Further, activity level, and position are influencing factors that should be considered in clinical practice.

Establishing Population Values for Chlorine Exposure in the United States (2015-2016) Using 2 Chlorine Biomarkers, 3-Chlorotyrosine and 3,5-Dichlorotyrosine.

In the United States, 12 million short tons of chlorine are manufactured and transported each year. Due to the volume of this volatile chemical, large- and small-scale chemical exposures occur frequently. To diagnose and treat potentially exposed individuals, reference range values for confirmatory biomarkers are required to differentiate between normal and abnormal exposure levels. Serum surplus samples (n = 1780) from the National Health and Nutrition Examination Survey (NHANES) 2015-2016 were measured for 2 chlorine biomarkers, 3-chlorotyrosine (Cl-Tyr) and 3,5-dichlorotyrosine (Cl2-Tyr), by liquid chromatography coupled to a triple quadrupole mass spectrometer. We evaluated demographic factors associated with elevated biomarker levels. Participant samples were analyzed for the chlorine biomarkers Cl-Tyr and Cl2-Tyr. In the unweighted analysis of these samples, 1349 (75.8%) were under the limit of detection (< LOD) of 2.50 ng/mL for Cl-Tyr and 1773 (99.6%) were < LOD for Cl2-Tyr. Samples within the method reportable range were 2.50 to 35.6 ng/mL for Cl-Tyr and 2.69 to 11.2 ng/mL for Cl2-Tyr. Since only 7 of the 1780 participants had detectable Cl2-Tyr, statistical analysis was limited to Cl-Tyr. Of the demographic characteristics examined, age, body mass index (BMI), estimated glomerular filtration rate (eGFR), and sex exhibited statistically significant differences in the weighted prevalence of detectable Cl-Tyr. This is the first reported set of Cl-Tyr and Cl2-Tyr population values for the United States. This population range coupled with NHANES demographic information could help healthcare professionals distinguish between normal and abnormal chlorine biomarker levels in an emergency. With this information, an inference could be made when determining acute chlorine exposure in individuals.

Compositional Analysis of Herbicide Tolerant and Conventionally Improved Soybeans in Nigeria

Aim/Background:Comparative compositional analysis was carried out between two herbicide tolerant soybean varieties and two conventionally improved non-GM soybeans (Glycine max) varieties for base line data and safety analysis for subsequent breeding purposes. Method:The experiment was laid in completely randomized design with three replications. The moisture content, ash content, nitrogen content, crude protein content, Percentage crude fibre content, crude lipid, metabolizable energy and calories values were determined for each of the samples using AOAC, 2000 method. Collected data was subjected to analysis of variance and pertinent means were separated using Fisher’s least significant difference (LSD) at 5% level of probability. Results:The moisture content values observed for the four varieties ranged from 5.0 – 5.9 % which were not significantly different from the standard reference of 6.12%. Ash content was lowest for TGX-1448-2E at 1.2%, followed by E 14017 at 4.2 %; while it was 4.7 % for TGX 1904-6F;.and the highest observed value was 6.42 % in E 14086. The least nitrogen content value was observed in E14017 at 6.12%, followed by TGX 1448-2E at 8.17 %; TGX 1904-6F at 8.9 % and the highest observed in E14086 at 11.6%. All values were within range of standard reference value of 496.37 Kcal/g except for TGX 1904-6F(363.09Kcal/g) which was relatively low. Conclusion:It could be concluded that contents of the key components of the HT soybean had no significant difference with those of the non-GM soybean ascertaining the safety and this data is necessary for onward varietal development.

Estimating reference values of parenchymal stiffness of normal pancreatic parenchyma by means of point shear wave elastography.

There are numerous imaging modalities available to describe pancreatic parenchyma. None of the broadly accepted diagnostic methods uses elasticity as an indicator of tissue damage.

Color Doppler Ultrasound of the Testis in Young Men with a Serum Testosterone Level below the Average Values of the Standard for a Prolonged Period

Introduction: This study aimed to correlate the testicular volume, ultrasound imaging, and blood flow parameters, determined using color Doppler ultrasonography in young men with a testosterone level below the average values of the standard for a prolonged period. Material and Methods: From January 2013 and December 2019, we examined 73 men aged 37 to 45 years with a testosterone level below the average values of the standard for a prolonged period and а 20 age-matched men with a testosterone level above the average values of the standard. We use color Doppler ultrasound as a method to evaluate the volume, ultrasound imaging and Doppler parameters of the testicular blood flow. Results: We obtained significantly lower but still within the reference range values for total testosterone (p&lt;0.001), compared to those of the control group. We found no differences in the ultrasound characteristics of the testicular parenchyma and significant differences between testicular volumes in patients with a testosterone level below the average values of the standard for a prolonged period and those in the control group. In the color Doppler ultrasound we recorded fluctuations only in the blood flow of a aa.intratesticularis, expressed in a slight increase in the resistance index. Conclusions: Ultrasonography of young men with a serum T level below the average values of the standard for a prolonged period found no reduction in testicular volume and no change in the ultrasound characteristic of the parenchyma, which suggests to us that this is not a congenital condition, but cannot answer the question of whether it will lead to structural changes in the testicles in the future and if so what. The non-significant increase in RI in the intratesticular arteries in all examined men is a non-specific indicator that directs the specialist’s attention to additional testing of total testosterone in patients with complaints from the male reproductive system.

HEMPSEED CAKE IN RABBIT NUTRITION: LIVESTOCK PERFORMANCES, QUALITY OF MEAT, DIGESTIBILITY OF NUTRIENTS AND ANIMAL HEALTH STATUS

The objective of this study was to evaluate the effect of dried hempseed cake (by-product of oil production) supplementation (5 % − EG1 and 10 % − EG2) to rabbit feed mixture on livestock performances, quality of meat, digestibility of nutrients and animal health status. The chemical composition of the Longissimus thoracis and lumborum muscle including the content of fatty acids and amino acids, and the biochemical parameters in caecum and blood of growing rabbits were investigated. No significant differences among the experimental groups in feed intake, body weight and carcass parameters were found. The digestibility trial was performed using the balance method. Feed mixtures differed in digestible energy content i.e., crude protein, crude fibre and fat. The resulting digestibility coefficients for protein fell within the range of 66.72 − 74.18 %, fat digestibility was in the interval from 88.73 to 89.34 % and crude fibre digestibility was in the range from 27.56 to 34.59 %. Fatty acid profile in intramuscular lipids represents the highest content of monounsaturated fatty acids (MUFA; 47.72 − 48.51 %), followed by the content of saturated acids (SFA; 33.75 − 33.87 %) and a low content of polyunsaturated fatty acids (PUFA; 11.23 − 12.08 %). Hempseed oil cake could be included in rabbits' diet with beneficial effect on carcass quality and can enhance the nutritional quality of rabbit meat with the focus on essential amino acids. The tested blood serum parameters were within the range of the physiological reference values with no statistical differences between experimental groups, except for glucose and cholesterol content. The data on volatile fatty acids (VFA) show that most intensive process was in the caecum of rabbits in the experimental groups EG1 and EG2. All obtained data let us to recommend the inclusion of hempseed cake up to 10 % in rabbit diet without any negative effect on animal welfare, livestock performance and quality of meat.

Comparative evaluation of the effectiveness of a prostaglandin analogue and fixed-dose combination drug containing brinzolamide 1% and timolol maleate 0.5% (an experimental study)

PURPOSE. Comparative evaluation of anti-hypertensive effectiveness of tafluprost 0.0015% (Taflotan), and a fixed-combination of benzolamide 1% and timolol maleate 0.5% (Azarga) preparation in an experimental animal model with artificially induced steroidal ophthalmic hypertension.METHODS. The study experiments were performed on 30 sexually mature Chinchilla rabbits (60 eyes) with steroid-induced glaucoma modeled by ocular instillations of dexamethasone.The animals were divided into 3 groups each consisting of 10 rabbits. The first group was the controls that were divided into two subgroups; they were instilled dexamethasone 0.1% two drops per day into the right eye (the left eye remained as control) for 20 days in subgroup 1, and for 30 days in subgroup 2.The animals in group 2 received Azarga, group 3 — Taflotan. Animals of group 2 and 3 were divided into three subgroups: in subgroup 1 the animals were instilled the studied drugs for 10 days, in subgroup 2 — for 20 days, in subgroup 3 — for 30 days.RESULTS. Both drugs reduced the intraocular pressure down to the reference range, i.e. 18–25 mm Hg. Animals that received instillations of Taflotan achieved the refe rence range values after 10 days, while the animals receiving Azarga — after 20 days.CONCLUSIONS. Under the conditions of steroidal ophthalmic hypertension, instillations of both Azarga and Taflotan yield satisfactory results in terms of IOP reduction. The use of Taflotan allowed achieving the reference range values after 10 days, Azarga — after 20 days. Taking into account the ease of administration of Taflotan, it can be recommended as the most convenient and effective drug in terms of reducing intraocular pressure.

ZAWARTOŚĆ SELENU W JAJNIKACH WOLNO ŻYJĄCYCH CERVIDAE Z TERENU PÓŁNOCNO-WSCHODNIEJ POLSKI

Selenium is counted among the trace elements necessary for the maintenance of metabolic processes occurring in the animal body, including the proper functioning of the reproductive system. The aim of the study conducted was to analyze the selenium content in the ovaries of red deer (Cervus elaphus) and European roe deer (Capreolus capreolus) in an attempt to establish a range of reference values for individuals of these species. Selenium concentrations in tissues tested were determined using spectrofluorometric method after wet mineralization in HNO3 and HClO4 mixture. Ovaries Se concentrations ranged from 0.034–0.338 mg⋅kg−1 wet weight and 0.015–0.285 mg⋅kg−1 wet weight in roe deer and red deer respectively. Based on the results, it was found that the average selenium content was higher in the ovaries of red deer and was 0.11 while in roe deer it was 0.09 mg⋅kg−1 wet weight. The gonads of European roe deer were characterized by higher variability of the analyzed pa- rameter in relation to samples obtained from female red deer. The coefficient of variation in their case was almost 90.1, while the value of the coefficient of variation for selenium content in the parenchymal layer of ovaries in red deer was almost 67.6.

269. Reference Data of Biochemical and Hematology Parameters in Juvenile and Adult Owl Monkeys (Aotus nancymaae) for Biomedical Research

Abstract Background Non-human primates (NHP), including New World monkeys (NWM), are used in varied fields of biomedical research encompassing infectious disease, behavioral sciences and drug and vaccine development. In these studies, normal reference values for biochemical and hematological parameters are often needed for comparison with experimental results. These blood parameters are useful guides in reaching a diagnosis, estimating the prognosis of the disease and monitoring the progress of treatment. Although there are various reports of biochemical and hematologic parameters in Old World monkeys, the range of reference values often differs between NHP species. Despite the importance of NWMs such as Aotus nancymaae in biomedical research, few studies have examined these values and with a limited number of animals only. A. nancymaae is widely used for research and often used to study the principal types of human malaria (P. falciparum and P. vivax). These primates have also been used to study leishmaniasis, schistosomiasis, tuberculosis and various types of enteric infections. Furthermore, in recent years oral challenge models have been developed in A. nancymaae for Campylobacter jejuni, Shigella flexneri, and Enterotoxigenic Escherichia coli (ETEC). However, there is no updated data on biochemical and hematological parameters for A. nancymaae. Therefore, the aim of this study was to provide complete and accurate reference intervals of biochemical and hematological values to assess the health status indices of A. nancymaae housed in a vivarium prior to their use. Methods To characterize these parameters, a total of 1250 blood samples (1003 from juvenile and 247 from adult monkeys) were analyzed between 2007 to 2022. A total of 14 biochemical and 20 hematology parameters were measured. Results A significant change was observed in the values of alkaline phosphatase (ALP), potassium (K+), glucose (GLU), blood urea nitrogen (BUN), creatinine (CRE), hemoglobin (HGB) and lymphocyte (LYM) in relation to sex and age. Conclusion In conclusion, this work establishes a comprehensive and systematic reference of serum biochemical and hematological parameters for A. nancymaae based on a large cohort which can be used to inform and guide future studies utilizing this animal model. Disclosures All Authors: No reported disclosures