Reduction Of Plant Fresh Weight Research Articles

Resistance screening of lentil cultivars against the root-knot nematode Meloidogyne incognita

Summary The root-knot nematode Meloidogyne incognita is a major soil parasite of lentil crops. Increasing restrictions of chemical nematicides have triggered a growing attention and interest in alternate root-knot nematode management. The present study was conducted to examine the level of resistance and/or susceptibility of five lentil cultivars (PL-456, KLS-218, Desi, DPL-62, Malika), grown in pots, against the root-knot nematode M. incognita. Root-knot nematode reproduction and host damage were assessed by recording the nematode infestation levels and reduction percentage of plant growth parameters. Nematode response and plant growth differentiated amongst the lentil cultivars. None of the cultivars was found immune or highly resistant. The cultivar Malika was found moderately resistant as it showed the lowest number of galls and egg masses/root as well as the lowest reduction of plant fresh weight (10.4%) and dry weight (6.9%). On the other hand, the cultivar Desi manifested the highest susceptibility exhibiting the highest number of galls and egg masses. There was a significantly negative correlation between the number of galls and plant growth parameters (plant fresh and dry weight and plant height).

Poison Ivy (Toxicodendron radican) Control with Dicamba and 2,4-D Applied Alone and in Tank Mixture

Poison ivy is a virulent weed that is frequently treated with herbicides. Dicamba, 2,4-D, and two fixed-ratio tank mixtures of dicamba plus 2,4-D were evaluated across a series of rates for poison ivy control. Objective was to test whether tank mixtures were more effective than either herbicide applied alone. Dicamba alone, 2,4-D alone, a 1 : 3, and a 3 : 1 ratio (by weight) mixture of dicamba plus 2,4-D, respectively, were applied at eight rates to 2-yr-old, container-grown poison ivy plants. The eight rates ranged from 0.036 to 1.79 kg ae ha−1, which, in terms of phytotoxicity, generally ranged from none to death. Percentage of control was determined from plant fresh-weight reduction relative to a nontreated control and was determined at 1 and 4 mo after treatment (MAT). Rates required for 95% control at 1 MAT and for control of regrowth at 4 MAT and the cost of those treatments were determined for the dicamba and 2,4-D applied alone and in the two mixtures. At the 1-MAT evaluation, 2,4-D alone was more cost effective than either dicamba alone or the two mixtures. By the 4-MAT evaluation, however, which followed clipping at 3 MAT, dicamba alone was more cost effective than either mixture. The 2,4-D alone failed to provide 95% control at the 4 MAT evaluation, even at the highest rate evaluated (1.79 kg ha−1). Response curves for the two mixtures were equivalent to the response curves of the components applied alone at the 1 MAT evaluation and fell between the response curves of the components at the 4 MAT evaluation. Hence, 2,4-D plus dicamba mixtures were neither antagonistic nor synergistic. Results indicate that dicamba applied alone is far more effective than 2,4-D is for control of established and perennial poison ivy, assuming the intent is to obtain control with a single, one-time application.

Poison Ivy (Toxicodendron radican) Control with Triclopyr and Metsulfuron, Applied Alone and in Tank Mixture

Dermatitis from poison ivy is an important health problem, and considerable effort is devoted to the control of this virulent weed. Triclopyr, metsulfuron, and two fixed-ratio tank mixtures of triclopyr and metsulfuron were evaluated across a series of rates for poison ivy control. The objective was to test whether tank mixtures are more effective than triclopyr alone. Triclopyr, metsulfuron, and 9 : 1 and 8 : 2 (by weight) mixtures of these two herbicides, respectively, were applied at eight rates to 1-yr old, pot-grown poison ivy plants. Rates ranged in phytotoxicity from none to death. Percentage of control as determined from plant fresh weight reduction relative to a nontreated control was determined at 1 and 4 mo after treatment (MAT). Data were subjected to ANOVA followed by nonlinear regression. Rates required for 95% control at 1 MAT, control of regrowth at 4 MAT, and the costs of these treatments were determined for the herbicides applied alone and the mixtures. Triclopyr alone and metsulfuron alone were consistently the least and the most expensive treatments, respectively. The mixtures were intermediate to these extremes.

Cost-Effectiveness of Glyphosate, 2,4-D, and Triclopyr, Alone and in Select Mixtures for Poison Ivy Control

Dermatitis from poison ivy is a significant health problem. Considerable effort is devoted to the control of this invasive and virulent weed in urban areas. Glyphosate, triclopyr, 2,4-D, a 1 : 1 mixture of glyphosate and 2,4-D, and a 9 : 1 mixture of glyphosate and triclopyr were evaluated for poison ivy control. Each of these three herbicides and two mixtures were applied at nine or ten rates, which ranged in phytotoxicity from none to death. Poison ivy plants had been propagated and container-grown. Percent control, as determined from plant fresh weight reduction, was determined at 1 and 4 mo after treatment (MAT). Data were subjected to ANOVA followed by nonlinear regression. Rates required for 95% control at 1 and 4 MAT and the associated costs were determined for each of the three herbicides and two mixtures. Acceptable control (i.e., ≥ 95%) at 1 and 4 MAT could be obtained at a much lower cost with either triclopyr or 2,4-D than with either glyphosate alone or with the two glyphosate-containing mixtures. Nonlinear regression also was used to evaluate whether the two mixtures were interactive (i.e., synergistic or antagonistic) or not (i.e., additive). Glyphosate plus triclopyr was synergistic for control at both 1 and 4 MAT. Glyphosate plus 2,4-D was synergistic for control at 4 MAT only. However, for both mixtures, synergism was only evident at rates that controlled poison ivy ≤ 80%. Both mixtures were noninteractive at rates required for acceptable control.

Resistance and biomass in Arabidopsis: a new model for Salicylic Acid perception

Salicylic acid (SA) is an essential hormone for plant defence and development. SA perception is usually measured by counting the number of pathogens that grow in planta upon an exogenous application of the hormone. A biological SA perception model based on plant fresh weight reduction caused by disease resistance in Arabidopsis thaliana is proposed. This effect is more noticeable when a chemical analogue of SA is used, like Benzothiadiazole (BTH). By spraying BTH several times, a substantial difference in plant biomass is observed when compared with the mock treatment. Such difference is dose-dependent and does not require pathogen inoculation. The model is robust and allows for the comparison of different Arabidopsis ecotypes, recombinant inbreed lines, and mutants. Our results show that two mutants, non-expresser of pathogenesis-related genes 1 (npr1) and auxin resistant 3 (axr3), fail to lose biomass when BTH is applied to them. Further experiments show that axr3 responds to SA and BTH in terms of defence induction. NPR1-related genotypes also confirm the pivotal role of NPR1 in SA perception, and suggest an active program of depletion of resources in the infected tissues.

Differential phytotoxicity of glyphosate in maize seedlings following applications to roots or shoot

SummaryThe transport and differential phytotoxicity of glyphosate was investigated in maize seedlings following application of the herbicide to either roots or shoots. One‐leaf maize seedlings (Zea mays L.) were maintained in graduated cylinders (250 mL) containing nutrient solution. Half of the test plants were placed in cylinders (100 mL) containing different 14C‐glyphosate concentrations; the remainder received foliar appliation of 14C‐glyphosate. After 26 h, the roots and the treated leaves were washed with distilled water, and the plants placed again in cylinders (250 mL) containing fresh nutrient solution for 5 days. Plants were weighed, and split into root, seed, cotyledon, coleoptile, mesocotyl, first leaf and apex. The recovery of 14C‐glyphosate was over 86%. For both application treatments, the shoot apex was the major sink of the mobilized glyphosate (47.9 ± 2.93% for root absorption and 45.8 ± 2.91% for foliar absorption). Expressed on a tissue fresh weight basis, approximately 0.26 μg a.e. g−1 of glyphosate in the apex produced a 50% reduction of plant fresh weight (ED50) when the herbicide was applied to the root. However, the ED50 following foliar absorption was only 0.042 μg a.e. g−1 in the apex, thus maize seedlings were much more sensitive to foliar application of the herbicide.