Reduction In Thickness Of Epithelium Research Articles

Redefining epithelial characterization in oral submucous fibrosis using morphometric analysis

Background:Oral submucous fibrosis (OSMF) is a chronic oral mucosal disease characterized by progressive deposition of collagen in subepithelial connective tissue and epithelial atrophy. The present study was conducted to evaluate the changes in epithelial thickness and cellular and nuclear morphometric characteristics of parabasal and spinous compartments of the epithelium in various stages of OSMF in comparison to normal epithelium.Materials and Methods:Sample included 30 cases of OSMF of various stages and five cases of normal buccal mucosa. Measurements for epithelial thickness and cellular morphology using morphometric analysis were performed on captured digital images using image analysis software.Statistical Analysis:The ANOVA analysis of epithelial thickness and correlation of epithelial thickness with cell contour and cell roundness was done using Karl Pearson's coefficient of correlation.Results:There was a statistically significant decrease in epithelial thickness with increase in severity/stage of OSMF. It was also observed that there is a highly statistically significant correlation (P = 0.000) between the thickness of epithelium and cell morphology in varying stages of OSMF.Conclusion:The morphometric parameters and the use of quantitative histological methods in determining the squamous epithelial structures thus made it possible to evaluate that there is reduction in thickness of epithelium or “atrophy” with increasing stages of OSMF which may be due to actual change in cell morphology of the individual cells of specific compartment or all compartments in the epithelium.

Histopathological Study on Induced Eeffects of Cadmium on Liver, Gizzard and Kidney of Larus argentatus

The study was carried out on the effects of cadmium (Cd) on liver, gizzard and kidney of Larus argentatus. Significant alterations were observed after the treatment of high and low dose of cadmium chloride injected to Larus argentatus. Investigation of the effects of Cd on body tissues was done by sectioning of fixed tissue by paraffin section techniques. Major changes were noted at high dose of cadmium chloride in liver, kidney and gizzard. While, under low dose of cadmium chloride, mild to moderate changes in hepatocytes around blood vessels in liver; reduction in thickness of epithelium and muscular layer in gizzard; and slight alterations in arrangement of tubules of loop of henle in the kidney of proximal convoluted tubules with granular cytoplasm were observed. No lesions were found in the mentioned body tissues of Larus argentatus of control group.