Pesticide Reduction Research Articles

Comparison of pesticide reduction in grape juice using ozone and UV-C light treatments

Comparison of pesticide reduction in grape juice using ozone and UV-C light treatments

Effect of intercropping apple trees with basil (Ocimum basilicum) or French marigold (Tagetes patula) on the rosy apple aphid regulation (Dysaphis plantaginea) and the abundance of its natural enemies.

In a global context of pesticide reduction, the sustainable management of aphids is a major challenge in maintaining economically viable fruit production. Intercropping with companion plants (CPs) that emit volatile organic compounds (VOCs) with repellent or attractive properties could be successful in the laboratory. Still, their effects on the orchard have been little documented. We tested in 2018 and 2019 the hypotheses that the introduction of basil or French marigold, decreases the populations of Dysaphis plantaginea Passerini, the rosy apple aphid (RAA) and increases the abundance of its natural enemies (NEs) in an apple orchard in the south of France. Laboratory tests confirmed that both CPs altered RAA reproduction performance. In orchards, they attracted more NEs around the pots and at some dates in the aphid colonies near the pots in both years (apart from basil in 2019), without a significant reduction in aphid populations in 2018. In contrast, in 2019, the number and spatial expansion of aphid colonies were significantly lower, close to basil. Further investigation is needed to disentangle the mechanisms explaining the observed effects (e.g., repellent action in autumn, etc.) but the results highlight the potential of CPs to control aphids in orchards and contribute to agroecological production of apple. Thus, this study shows the potential of CPs in a strategy combining aphid repellency and NEs attraction to control aphids in orchards, provided that CP installation lasts over several consecutive years. © 2024 Society of Chemical Industry.

Capturing the effects of flower strips on natural pest control in agronomic land use models

ContextGiven policy objectives for pesticide reduction, functional biodiversity is increasingly important for agricultural production. However, economic land use optimisation models rarely account for the interplay between agricultural production and functional biodiversity, like natural pest control (NPC). ObjectiveWe present and discuss an approach to consider feedback effects between NPC and agricultural production in a geodata-based farm economic land use model. MethodsThe geodata-based land use model PALUD was extended to incorporate interactions between NPC and crop yields. This included linking a generic NPC model, based on semi-natural habitats, with an econometric approach to estimate yield gaps as a function of NPC potential. As a case study, the model was then applied to the German state Brandenburg, using permanent flower strips as example to promote NPC. Here, we analysed the impacts of enhancing NPC potential on food production and gross margins from arable land use. Results and conclusionsOur analysis revealed that a higher level of NPC through flower strips improved gross margins. by up to 6.4 % and crop production by up to 1.2 % within a pesticide-free environment in Brandenburg. Despite inherent limitations and uncertainties of the approach, our study provides a pivotal advancement in integrating NPC into economic land use models. Future refinements incorporating more detailed knowledge on context-specific interactions between crops, pests and predators will improve model robustness. Our approach and case study results provide valuable insights for policy-making on tailored sustainable landscape development and serve as a foundation for future modelling efforts. SignificanceTo our knowledge the interaction between NPC and crop yields has not yet been integrated into any geospatially based economic land use model before. Incorparating these interactions is vital for guiding policies towards sustainable agricultural systems. In this respect it is important to develop and discuss further methodological approaches.

The Impact of Agricultural Socialization Services on the Ecological Protection of Rice Farmland in Jianghan Plain, China

The ecological protection of cultivated land is crucial for advancing high-quality agricultural development. This study analyzes the impact of agricultural socialization services on the ecological conservation of farmland, focusing on the reduction of chemical fertilizers and pesticides among rice farmers in the Jianghan Plain area. Utilizing data from 743 farmer household surveys conducted in 2023, the findings reveal that agricultural socialization services significantly encourage farmers to reduce chemical input usage. For every 1% increase in the level of agricultural social services, the average fertilizer use per hectare will decrease by 14% and the average pesticide use per hectare will decrease by 16.4%. The study identifies scale operation, factor substitution, and alleviation of capital constraints as mediating factors enhancing the efficacy of these services. Furthermore, heterogeneity analysis indicates that these services are more effective in reducing chemical inputs among large-scale and newer-generation farmers compared to their small-scale and older counterparts. Additionally, technology-intensive socialized services exhibit a stronger impact on reducing chemical inputs than labor-intensive services.

Legal Barriers in Sustainable Agriculture: Valorization of Agri-Food Waste and Pesticide Use Reduction

The transition to sustainability in agriculture faces significant challenges, especially to balance environmental goals with the practical demands of food production. This paper examines two different case studies that reveal the complexities of agricultural regulation. The first case focuses on the valorization of agri-food residual biomasses, highlighting the potential to transform food waste into valuable bioproducts such as bioenergy and biofertilizers. Despite the clear environmental and economic benefits, the absence of specific European regulations hinders the widespread adoption of these practices. Without clear rules for achieving “end-of-waste” status, the development and marketing of bio-based products remain restricted. The second case study examines the European Union’s unsuccessful effort to implement the Sustainable Use of Pesticides Regulation (SUR), which aimed to reduce pesticide use by 50% by 2030. Although the regulation sought to align agricultural practices with the EU’s Green Deal, it triggered widespread protests from farmers concerned about the potential economic losses and decreased productivity. These two cases, one showing under-regulation and the other over-regulation, highlight the need for balanced and practical regulatory frameworks that promote sustainability without imposing unrealistic demands on stakeholders. This paper ends with recommendations to harmonize regulations across Europe, ensuring that both innovation in agricultural waste management and practical pesticide reduction strategies are implemented in a way that supports farmers and producers, minimizing economic disruptions and encouraging sustainable agricultural practices.

Development of point-of-need colourimetric, isothermal diagnostic assays for specific detection of Bacillus subtilis using shikimate dehydrogenase gene.

The largest obstacle in the promotion of biopesticides is the existence of counterfeit products available in the market. Identification and quantification of antagonistic organisms in biopesticide products are the key to the reduction of spurious microbial pesticides. In this study, we have developed a simple, sensitive, isothermal-based colourimetric assay for specific detection of Bacillus subtilis from the biopesticide formulations and soil samples. A region specific to B. subtilis which codes for shikimate dehydrogenase was identified through in silico analysis. We employed conventional PCR, loop-mediated isothermal amplification (LAMP), recombinase polymerase amplification (RPA), and qPCR for specific detection of B. subtilis in soil samples and biopesticide formulations. Specificity tests showed that the PCR primers amplified an amplicon of 521bp in four strains of B. subtilis only, and no amplification was found in negative control samples. Similarly, the LAMP assay showed sky blue colour in all four strains of B. subtilis and violet colour in negative control samples. Whereas in the RPA assay, upon the addition of SYBR Green dye, a bright green colour was seen in B. subtilis strains, while a brick-red colour was observed in negative control samples by visualizing under a UV transilluminator. The qPCR assay showed specific amplifications with a Ct value of 12 for B. subtilis strains and no amplification in negative control samples. In the sensitivity test, PCR could amplify DNA of B. subtilis up to 500pg/µL. DNA concentration as low as 10pg/µL was enough to show the colour change in the LAMP as well as the RPA assays, whereas the qPCR assay showed sensitivity till 100pg/µL. All four diagnostic assays developed in the study have been validated in soil samples and B. subtilis-based biopesticides. Compared to conventional PCR, the qPCR assay has the advantage of quantification and visualizing the result in real-time, whereas LAMP and RPA assays have the benefits of being colourimetric and less time-consuming. The other advantages are that the results can be visualized with the naked eye, and these assays do not require a costly thermal cycler and gel documentation system. Hence, LAMP and RPA assays are highly suitable for developing point-of-need diagnostic kits and, in turn, help regulators assess the quality of biopesticides in the market.

Not the average farmer: Heterogeneity in Dutch arable farmers’ intentions to reduce pesticide use

Ambitious environmental policies and regulations in Europe aim to reduce pesticide use, yet their implementation faces significant obstacles. Effective strategies that gain support within the farming community require a deeper understanding of the underlying intentions, considering that farmers are a heterogeneous group with diverse beliefs related to socio-demographic characteristics. Using an existing dataset with theory of planned behaviour data from 359 Dutch arable farmers (Bakker et al., 2021), we examined the heterogeneity in intentions and beliefs regarding pesticide reduction. Expanding the analysis with quantile regression models, we show that the influence of attitude becomes increasingly important as farmers' aspirations to reduce pesticide use grow. Additionally, we observed a small positive effect of injunctive norms at the 25th quantile and a small negative effect at the 75th quantile of intention. These findings indicate that the relative impact of these constructs varies across the intention distribution, emphasising the need for more nuanced quantitative analyses of heterogeneity in TPB studies. Using moderation models, we observed variations in the relative impact of attitude, injunctive and descriptive norms on intention across different segments of the farming community, particularly concerning age, educational level, and farm income dependencies. Younger, higher-educated farmers, and those less reliant on farm income demonstrated greater openness towards reducing pesticide usage and adopting alternative crop protection practices. These findings suggest that different farmer segments may respond differently to interventions and incentives. Policymakers can leverage this knowledge to develop more nuanced and targeted strategies that promote pesticide reduction while aligning with the diverse motivations and beliefs present among farmers.

Impact of Ecological Cognitive Bias on Pesticide Reduction by Natural Rubber Farmers in China: Insight from Price Insurance Satisfaction

Some natural rubber farmers mistakenly equate the ecological functions of rubber plantations with those of primary forests. This cognitive bias can hinder pesticide reduction efforts. Meanwhile, natural rubber farmers gain security through price insurance, which helps them adopt a long-term perspective on environmental protection, mitigating the negative impact of cognitive biases on pesticide use decisions. However, existing research often overlooks the influence of ecological cognitive bias on pesticide reduction and the moderating role of price insurance satisfaction. This study utilizes field survey data from Hainan and employs logit models and double machine learning models to empirically analyze the impact of ecological cognitive bias on pesticide reduction among natural rubber farmers. It further tests the underlying mechanisms using moderation models. The results indicate that (1) ecological cognitive bias negatively affects pesticide reduction among natural rubber farmers, and (2) price insurance satisfaction mitigates the impact of ecological cognitive bias on pesticide reduction. Based on these findings, the government should enhance education and training to raise ecological awareness among natural rubber farmers and reduce ecological cognitive biases. Disseminating knowledge about price insurance and establishing a comprehensive insurance system can secure farmers’ income and promote the rational use of pesticides.

Selected farm-level crop protection practices in Europe and Argentina: Opportunities for moving toward sustainable use of pesticides

Extensive use of plant protection products (PPP) in the last decades contributes to negative impacts on ecosystems, animals and humans. For the strategies of PPP reduction and replacement of hazardous pesticides, farm-level data on agronomic management practices and crop protection applications are crucial. In this study, we strategically collected data for the 2021 season at the SPRINT project case study sites (CSS) in 10 European countries and Argentina, on perennial, arable and vegetable crops. Data collection included strategically selected farm and field data, pesticide records and farming practices. Results involved more than 1700 recorded PPP applications across various crops with more than 170 different active substances from PPP in organic, integrated pest management and conventional farming practices. We explored differences in application patterns (fungicides, insecticides, herbicides and non-PPP, e.g. adjuvants, growth regulators, and fertilizers) between and within crops, countries and farming systems and calculated the costs of PPP use. The pesticide dosages applied during the crop season varied up to a factor of 20 around recommended doses. Regarding the costs of PPPs use perennial crops had the highest costs per ha crop production area. Finally, we analysed the active substances applied in different farming systems in terms of their hazard statements. Our results shed light on how PPPs are used across different crop and farming types and will help elucidate how pesticide application patterns can be changed in the future. Finally, we highlighted non-PPP use practices which help to reduce dependency on PPP use. This might be used to support decision-making and policies within agricultural advisory/support systems.

Pesticide Reduction through Organic Farming for Promoting Public Health Management and Food Security

There is a growing global interest in sustainable food consumption to ensure food security. One significant factor driving this demand is the belief that consuming organic food affects Public Health (PH). This research examines the historical context of sustainable agriculture. This article specifically examines the impact of sustainable agriculture on health-related substances, pesticide residues, and contaminants in crops. It also explores the connections between organic food and health indicators. In Organic Farming (OF), the use of pesticides is often restricted or non-existent, which is different from Traditional Farming (TF) methods that heavily depend on pesticides to preserve crops. The notable disparities in pesticide usage between the two agricultural systems profoundly affect the comparative nutritional exposure, hazards, and ecological impacts associated with pesticides. Pesticide Usage Monitoring (PUM) information has been used to compare pesticide usage across organically certified and adjacent traditional farms for tomato crops. This work suggested methods for PR by promoting the widespread use of organic agricultural techniques. Additionally, emphasis on many alternatives available within organic food supply chains to further minimize the usage of pesticides, contact, and adverse effects on workers has been proposed to provide food security.

How does pesticide reduction affect labour time and profitability? A crop production case study

CONTEXTNational and international agendas are focusing on reducing pesticides due to their detrimental effects on flora, fauna, and human health, which has led to the introduction of agri-environmental programmes aimed at reducing the risk of pesticides. Pesticide reduction in agriculture can have an impact on labour time requirements and profitability. OBJECTIVEWe used winter wheat, sugar beet, and potatoes as examples to analyse the changes in profitability and working time requirements, including management tasks. METHODSFor the calculations, we used five different production schemes for each crop: reference; (A) reduction of herbicides; (B) reduction of growth regulators, fungicides, and insecticides; combination of schemes (A) and (B); and organic production. The working time requirements for fieldwork and farm management work were modelled for each scheme and crop. The respective partial costs and benefits of the schemes were calculated for each crop. RESULTS AND CONCLUSIONSBased on the model assumptions, scheme (B) appears favourable in terms of working time requirements, and profitability of winter wheat and sugar beet. Scheme (A) offers synergies between the same parameters for potato production. Economic analysis shows that crop production with reduced pesticide use may even experience an increase in financial viability if the yield is not severely jeopardised, and farmers can be compensated through premiums and direct payments. SIGNIFICANCEOur results can support policy-making, since the labour time requirement and profitability of pesticide-reduced crop production can affect the success of voluntary agri-environmental programmes for the reduction of the risks from pesticide use in agriculture.

The TIMES Land-WEF model: An integrated analysis of the agricultural system of the Basilicata Region (Southern Italy)

The unsustainable use of natural resources, in particular soil degradation and pollution, is one of the main factors contributing to the climate and biodiversity crisis. The European Union has outlined a new European Green Deal, whose objectives include increasing the overall quality of the agri-food chain in relation to environmental sustainability, focusing on reducing the use of pesticides and increasing the share of organic in overall production. A Nexus thinking perspective is applied to analyse this topic over a 50-year time horizon (2010–2060) for the agricultural system of the Basilicata Region (Southern Italy), represented by the TIMES Land-WEF, an optimizing, bottom-up energy-technology model, built to investigate the interactions and interrelations between water, energy food and land. The novelty of this modelling approach is the choice of land use as the guiding parameter of the optimization process. The main objectives of the Farm to Fork Strategy are modelled as system constraints and the scenario analysis allows to characterise their effects on the evolution of the agricultural system over the examined time. The results show that the pesticide reduction constraint leads to an increase in land use by organic crops from 24.6 % to 32.4 % in 2060. In particular, this is due to the increased contribution of cereal, forage, olive growing crops, permanent meadows and pastures, which lead to a 46 % reduction in irrigation water consumption. On the other hand, the reduction in inorganic fertilizers is not accompanied by a significant increase in organic crops, but resulted in the reduction of cereal crops.

Landscape features support natural pest control and farm income when pesticide application is reduced

Future trajectories of agricultural productivity need to incorporate environmental targets, including the reduction of pesticides use. Landscape features supporting natural pest control (LF-NPC) offer a nature-based solution that can serve as a partial substitute for synthetic pesticides, thereby supporting future productivity levels. Here, we introduce a novel approach to quantify the contribution of LF-NPC to agricultural yields and its associated economic value to crop production in a broad-scale context. Using the European Union as case study, we combine granular farm-level data, a spatially explicit map of LF-NPC potential, and a regional agro-economic supply and market model. The results reveal that farms located in areas characterized by higher LF-NPC potential experience lower productivity losses in a context of reduced synthetic pesticides use. Our analysis suggests that LF-NPC reduces yield gaps on average by four percentage points, and increases income by a similar magnitude. These results highlight the significance of LF-NPC for agricultural production and income, and provide a valuable reference point for farmers and policymakers aiming to successfully invest in landscape features to achieve pesticides reduction targets.

Electrochemical Enrichment of Biocharcoal Modified on Carbon Electrodes for the Detection of Nitrite and Paraxon Ethyl Pesticide

Biocharcoal (BioC), a cost-effective, eco-friendly, and sustainable material can be derived from various organic sources including agricultural waste. However, to date, complex chemical treatments using harsh solvents or physical processes at elevated temperatures have been used to activate and enhance the functional groups of biochar. In this paper, we propose a novel easy and cost-effective activation method based on electrochemical cycling in buffer solutions to enhance the electrochemical performance of biocharcoal derived from almond shells (AS-BioC). The novel electrochemical activation method enhanced the functional groups and porosity on the surface of AS-BioC, as confirmed by microscopic, spectroscopic characterizations. Electrochemical characterization indicated an increase in the conductivity and surface area. A modified SPCE with activated AS-BioC (A.AS-BioC/SPCE), shows enhanced electrochemical performance towards oxidation and reduction of nitrite and paraxon ethyl pesticide, respectively. For both target analytes, the activated electrode demonstrates high electrocatalytic activity and achieves a very LOD of 0.38 µM for nitrite and 1.35 nM for ethyl paraxon with a broad linear range. The sensor was validated in real samples for both contaminants. Overall, the research demonstrates an innovative technique to improve the performance of AS-BioC to use as a modifier material for electrochemical sensors.

Causal inference and mechanism for unraveling the removal of four pesticides from lettuce (Lactuca sativa L.) via ultrasonic processing and various immersion solutions

This study explores the reduction of carbamates (CAs) and pyrethroids (PYs) − commonly used pesticides − in lettuce using various immersion solutions and ultrasonic processing. It also examines the role of machine learning and molecular docking in understanding the mechanisms of pesticide reduction. The results revealed that the highest reduction of both CAs and PYs exceeded 80 % on lettuce leaves. In most samples, the reduction increased with the power of ultrasonic processing and processing time. The results of machine learning models (XGBoost and SHAP) showed that during the immersion cleaning of CAs and PYs, as well as during both immersion cleaning and ultrasonic processing of CAs + PYs, the reduction was most influenced by the initial pesticide levels and immersion time. Gas Chromatography-Mass Spectrometry (GC–MS) analysis of lettuce’s wax layer identified 24 compounds, including fatty alcohols, fatty acids, fatty acid esters, and triterpenoids. Despite the absence of active sites, the lipophilic nature of long-chain aliphatic compounds aids in pesticide binding, while triterpenoids form strong hydrogen bonds with pesticides, indicating a robust adsorption on the lettuce surface. This study aims to offer insights into the efficient removal of chemical pesticide residues from fruits and vegetables, addressing critical concerns for food safety and human health.

Pesticide reduction: clustering organic croplands

Organic and conventional farms often coexist, yet their proximity does not ensure compatibility. Larsen et al. reveal that being surrounded by organic fields reduces pesticide usage in organic fields but increases it in conventional fields. We discuss these findings, emphasizing the need to cluster organic croplands for reduced pesticide use.

Efficacy of selected botanicals, phosphine and boric acid against pulse beetle, Callosobruchus chinensis under laboratory conditions

Insect pests are a threat to both growing field crops and stored grain commodities during storage. There are different stored pests which affect the mung bean (Vigna radiata) all over the world. Most important destructive pest is Pulse beetle Callosobruchus chinensis which cause lot of losses during storage of mung bean. Different pesticides are being used to control the Pulse beetle but these pesticides have harmful effect on human health, grain quality and development of insect resistant population. The replacement approaches for pest management in food items is a growing need in the food sector, which should match customer requests for pesticide reduction or eradication. In the present study impact of phosphine, boric acid and botanicals (Neem and Eucalyptus) on adult stage of pulse beetle Callosobruchus chinensis was evaluated at 65 ±5% RH, 30±1°C. The pulse beetles were exposed to phosphine, boric acid at the different concentrations like phosphine 2g, 1g, & 0.5g, boric acid 0.25g, 0.12g & 0.06g and botanicals leaves 2g, 5g and 10g for 24h, 48h and 72h was evaluated. Maximum mortality (98%) by phosphine at 0.25 g, boric acid (95%) at 0.25 g, neem leaves (96%) at 10g and eucalyptus (95%) at 10g after 72h was observed. This technique is a best and eco-friendly to controlling pulse beetles. This technique has no any adverse or residual effect on the quality of grains.

Development of Regenerative Tea Cultivation Models through Dual Approach of Soil and Plant Health Management towards Crop Sustainability, Soil Quality Development, Pesticide Reduction and Climate Change Mitigation: A Case Study from Lakhipara Tea Estate,

Climate change is a reality and its impact on the tea plantations is apparent in the rise of pest intensity, higher agrochemical use, inconsistent crop yields, declining grand growth period, increased abiotic stress and various other challenges; that threaten long-term sustainability of the Indian tea sector. The urgency to adopt sustainable practices is increasing by the day but to gain time bound results a comprehensive focus encompassing soil and plant health development will be crucial. To deal with climate challenge head-on, the Indian tea industry is making changes at various levels of operations. The sustainable tea initiative at Lakhipara tea estate, was one such attempt by the Goodricke Group Limited in their Dooars tea growing region of West Bengal. The program was initiated in 2014 with an aim to reduce pesticide use, improve soil quality, and produce quality teas while sustaining crop yields, improving renewable energy use and lowering the carbon footprint. Adoption of Inhana Rational Farming (IRF) Technology and taking the essence from ‘Trophobiosis Theory’ of French Scientist F. Chaboussou, the program focused on management of soil and plant health. Three years evaluation of crop yields in respect of the budgeted crop, revealed on an average an excess of 78 kg/ha/year in the project area. In rest of the garden area a contrasting crop loss of 118 kg was recorded per ha during the same period. The higher crop performance positively correlated with the higher nutrient use efficiency which was 17.7 percent higher in the project area (NUENPK : 8.86) as compared to the general garden area (NUENPK : 7.53), which reflected the impact of plant health management towards enhanced nutrient uptake, assimilation and utilization. Assessment of pesticide usage, revealed up to 77% decrease in usage in the project area during 2014-16, as compared to the pre- project year. Comparison with pesticide usage of the Dooars tea growing region during this same period, indicated a 62% lower Crop Pesticide Pollution Index (CPPI) in the project area. The finding indicated a 52 to 77% reduction in the accumulated toxicity potential of the applied pesticides in the project area; thereby accrediting safer tea development under this program, when adjudged in terms of pesticide residue. Assessment of soil quality revealed an overall eight percent increment in Soil Fertility Index (FI) value, with significant improvement in soil microbial activity potential (MAP) values i.e., by almost four times. The finding pointed towards the favourable impact of soil health management primarily through Novcom composting towards enhancement of soil microbial interactions. Post three years of experimentation the overall Soil Quality Iindex (SQI) value increased by 6% in the project area. The finding corroborated a concurrent 6.72% increase in the soil organic carbon stock during the same period. Reduction in use of non- renewable inputs viz. chemical fertilizers and pesticides in the project area was indicated by approximately 40 percent enhancement in energy use efficiency and energy productivity post the assessment period. Carbon assessment in terms of kg CO2 equivalents/ kg made tea (using ACFA version 1.0) indicated approx. 65 to 70 % lower footprint in the project area, primarily due to 20 to 30 % reduction in chemical fertilizers and 60 to 70 % reduction in the use of synthetic pesticides. The results indicated that while integrated soil management is the pre-requisite criteria towards rejuvenation of soil health and for restoration of the habitat for predators, it does not play a direct role in reducing the pest pressure and simultaneously the requirement of pesticides. Physiologically activated plants/ bushes on the other hand; due to their higher nutrient assimilation capacity and efficient protein synthesis are always lesser susceptible to pest attack. Hence, focus on activation of plant physiology can reduce the plant- pest interaction leading to a natural reduction in the requirement for pesticides vis-à-vis the pesticide usage.

Development of Regenerative Tea Cultivation Models through Dual Approach of Soil and Plant Health Management towards Crop Sustainability, Soil Quality Development, Pesticide Reduction and Climate Change Mitigation: A Case Study from Lakhipara Tea Estate, Dooars, West Bengal, India (PART-II)

The first part of this research article documents the impact of the studied Regenerative Agriculture models in tea in respect of crop sustainability, pesticide load reduction and reduction of pest management cost. The findings indicated an increase (78 kg/ha) in crop productivity in the project area as against crop loss of 118 kg/ha in the non- project area during the same period. The finding also indicated a 52 to 77% reduction in the accumulated toxicity potential of the applied pesticides, improvement of soil quality indices and a 6.72% increase in the soil organic carbon stock. Most importantly, carbon assessment in terms of kg CO2 equivalent/ kg made tea (using ACFA version 1.0) indicated approximately 65 to 70 % lower footprint in the project area. The lowering of carbon footprint was due to a 20 to 30 % reduction in the chemical fertilizers along with improvement in carbon sequestration potential of the soil due to quality compost application and reduction of herbicides. The study indicated that Inhana Rational Farming (IRF) Technology can serve as an effective tool towards development of resource based Regenerative Agriculture Models that can ensure safe and low carbon tea cultivation without compromising crop yield and without increasing the cost of cultivation.

Size-dependent Effect on Foliar Utilization and Biocontrol Efficacy of Emamectin Benzoate Delivery Systems.

The development of nanopesticides provides new avenues for pesticide reduction and efficiency improvement. However, the size effect of nanopesticides remains unclear, and its underlying mechanisms of influence have become a major obstacle in the design and application of pesticide nanoformulations. In this research, the noncarrier-coated emamectin benzoate (EB) solid dispersions (Micro-EB and Nano-EB) were produced under a constant surfactant-to-active ingredient ratio by a self-emulsifying-carrier solidification technique. The particle size of Micro-EB was 162 times that of spherical Nano-EB. The small size and large specific surface area of Nano-EB facilitated the adsorption of surfactants on the surface of the particles, thereby improving its dispersibility, suspensibility, and stability. The pinning effect of nanoparticles significantly suppressed droplet retraction and rebounding. Moreover, Nano-EB exhibited a 25% higher retention of the active ingredient on cabbage leaves and a 70% higher washing resistance than Micro-EB, and both were significantly different. The improvement of abilities in wetting, spreading, and retention of Nano-EB on crop leaves contributed to the increase in foliar utilization, which further resulted in a 1.6-fold enhancement of bioactivity against target Spodoptera exigua compared to Micro-EB. Especially, Nano-EB did not exacerbate the safety risk to the nontarget organism zebrafish with no significant difference. This study elaborates the size effect on the effectiveness and safety of pesticide formulations and lays a theoretical foundation for the development and rational utilization of efficient and environmentally friendly nanopesticides.