Reduced Malondialdehyde Levels Research Articles

Co-Treatment with Cranberry and Vitamin-C Mitigates Reproductive Toxicities Induced by Phenobarbital in Male Rats

Background/Aims: Phenobarbital (PB), commonly used for epilepsy management, is associated with testicular dysfunction after prolonged use. This study aimed to evaluate the ameliorative effects of cranberry (CB) and vitamin C (Vit-C) on PB-induced reproductive toxicity in rats. Methods: Forty male Wistar rats were divided into five groups. G1 was the negative control, while G2 received PB (160 mg/kg orally) for one month. Groups G3 and G4 received PB followed by CB (500 mg/kg) and Vit-C (27 mg/kg) treatments, respectively. G5 received PB followed by a combined CB and Vit-C regimen. The levels of catalase (CAT), superoxide dismutase (SOD), glutathione reduced (GSH), and malondialdehyde (MDA) were determined using standard biochemical assays. Histological changes in testicular tissues were assessed, and caspase-3 expression was quantified via immunohistochemistry. Results: PB exposure increased MDA levels, reduced SOD and CAT activity, and disrupted testicular histology, with elevated caspase-3 expression indicating heightened apoptosis. Treatment with CB or Vit-C significantly restored antioxidant enzyme activities, reduced MDA levels, and ameliorated histological abnormalities. Co-treatment with CB and Vit-C yielded the most pronounced protective effects, including reduced caspase-3 expression and improved testicular structure. Conclusion: CB and Vit-C demonstrate significant protective effects against PB-induced testicular toxicity, likely due to their antioxidative and anti-apoptotic properties. Co-administration of these agents offers an effective strategy to mitigate reproductive toxicities associated with prolonged PB use.

Tongmai Hypoglycemic Capsule Attenuates Myocardial Oxidative Stress and Fibrosis in the Development of Diabetic Cardiomyopathy in Rats.

To investigate the effect of Tongmai Hypoglycemic Capsule (THC) on myocardium injury in diabetic cardiomyopathy (DCM) rats. A total of 24 Sprague Dawley rats were fed for 4 weeks with high-fat and high-sugar food and then injected with streptozotocin intraperitoneally for the establishment of the DCM model. In addition, 6 rats with normal diets were used as the control group. After modeling, 24 DCM rats were randomly divided into the model, L-THC, M-THC, and H-THC groups by computer generated random numbers, and 0, 0.16, 0.32, 0.64 g/kg of THC were adopted respectively by gavage, with 6 rats in each group. After 12 weeks of THC administration, echocardiography, histopathological staining, biochemical analysis, and Western blot were used to detect the changes in myocardial structure, oxidative stress (OS), biochemical indexes, protein expressions of myocardial fibrosis, and nuclear factor erythroid 2-related faactor 2 (Nrf2) element, respectively. Treatment with THC significantly decreased cardiac markers such as creatine kinase, lactate dehydrogenase, and creatine kinase-MB, etc., (P<0.01); enhanced cardiac function indicators including heart rate, ejection fraction, cardiac output, interventricular septal thickness at diastole, and others (P<0.05 or P<0.01); decreased levels of biochemical indicators such as fasting blood glucose, total cholesterol, triglycerides, low-density lipoprotein cholesterol, aspartate transaminase, (P<0.05 or P<0.01); and decreased the levels of myocardial fibrosis markers α-smooth muscle actin (α-SMA), and collagen I (Col-1) protein (P<0.01), improved myocardial morphology and the status of myocardial interstitial fibrosis. THC significantly reduced malondialdehyde levels in model rats (P<0.01), increased levels of catalase, superoxide dismutase, and glutathione (P<0.01), and significantly increased the expression of Nrf2, NAD(P)H:quinone oxidoreductase 1, heme oxygenase-1, and superoxide dismutase 2 proteins in the left ventricle of rats (P<0.01). THC activates the Nrf2 signaling pathway and plays a protective role in reducing OS injury and cardiac fibrosis in DCM rats.

Indole-3-propionic acid enhances growth performance and reduces diarrhea via modulating redox status and intestinal inflammation in weaned piglets

Indole-3-propionic acid (IPA) has anti-inflammatory properties, which can be beneficial for weaned piglets with underdeveloped immune systems. The study explores the impact of IPA supplementation on growth performance, oxidative stress, and inflammation response in weaned piglets. In experiment (Exp.) 1, ninety weaned piglets were divided into six groups (five replicates per group, three pigs per replicate), with each group receiving a basal diet with varying amounts of IPA (0, 50, 100, 200, 400, or 600 mg/kg) for 42 d. Piglets fed the diets with 50, 100, and 200 mg/kg of IPA exhibited reduced feed conversion ratios (F: G) compared to the control piglets (P = 0.035). Notably, 50 and 100 mg/kg IPA treatments significantly reduced diarrhea incidence and serum interleukin (IL)-6 content (P < 0.05). Conversely, a high dosage of 600 mg/kg IPA led to increased serum contents of tumor necrosis factor (TNF)-α, and IL-6 (P < 0.05). Optimal antioxidant benefits were observed at 100 mg/kg IPA supplementation, which significantly reduced malondialdehyde levels while enhancing serum total antioxidant capacity and total superoxide dismutase activity on d 14 (P < 0.05). Exp. 2 investigated the effects of IPA on lipopolysaccharide (LPS) challenge in weaned piglets. The study consisted of 32 weaned piglets allocated into 4 groups, with 8 replicates per group and 1 piglet per replicate: a control group, a LPS challenge group, a LPS challenge group supplemented with 100 mg/kg IPA, and a group supplemented with 100 mg/kg IPA alone. Upon administration of LPS or saline injection, the results indicated that dietary IPA supplementation in challenged piglets enhanced villus height: crypt depth, modulated IL-8 and IL-22 mRNA relative expression, and increased the tight junction protein claudin-1 mRNA relative expression in the intestinal mucosa (P < 0.05). These findings suggest that dietary supplementation of IPA at specific concentrations significantly improves growth performance, reduces diarrhea incidence, and mitigates inflammation and oxidative stress in weaned piglets. It may be concluded that incorporating IPA into the diet of weaned piglets can effectively improve their health and development.

Efficacy of lycopene in reducing malondialdehyde (MDA) levels due to Lipopolysaccharide (LPS) exposure in Mice (Mus musculus)

Objective: Lipopolysaccharide (LPS) is a major component of the outer membrane of gram-negative bacteria and can trigger inflammatory and oxidative stress responses. This study aims to observe the efficacy of lycopene in reducing malondialdehyde (MDA) levels due to lipopolysaccharide (LPS) exposure in mice (Mus musculus). Method: This laboratory experimental study used 25 male mice aged 3 months with body weights of ± 25 g – 35 g, divided into five groups with five replicates each. K(-) was the control group: no LPS or lycopene was administered. K(+): received LPS 0.042 mg/kg without lycopene. P1: received LPS 0.042 mg/kg and lycopene 0.3 mg/kg. P2: received LPS 0.042 mg/kg and lycopene 0.6 mg/kg. P3: received LPS 0.042 mg/kg and lycopene 0.9 mg/kg. LPS was given on days 1 and 8 intraperitoneally, and lycopene was administered daily for 14 days. MDA levels were measured using the ELISA method, and data analysis was performed using ANOVA followed by Tukey's Test (p&lt;0.05). Result: LPS exposure significantly increased MDA levels (p&lt;0.05), and lycopene administration significantly reduced MDA levels (p&lt;0.05) to normal levels similar to the control (-). Conclusion: LPS exposure significantly increased MDA levels, and lycopene administration was able to reduce MDA levels to normal limits.

Duguetia furfuracea (A.ST. Hil.) Saff.: Neuroprotective Effect on Chemically Induced Amnesia, Anxiolytic Effects and Preclinical Safety Evaluation in Mice

Duguetia furfuracea, “araticum-seco”, is known to contain several bioactive compounds that can mitigate oxidative stress and act on the central nervous system (CNS). This effect is partly attributed to its potent antioxidant and acetylcholinesterase (AChE) inhibitors. In this study, the effects were explored of the methanolic extract (MEDF) and alkaloid fraction (AFDF) of D. furfuracea (leaves) on cognitive behaviors in male mice with scopolamine (Scop)-induced cognitive impairment and biochemical parameters. Additionally, anxiolytic behavior, subacute toxicity, molecular docking and antioxidant activity were reported. MEDF (30, 100 or 300 mg/kg) or AFDF (30 mg/kg) were orally administered for 16 days and Scop (intraperitoneally, i.p.) between days 11 and 16. The anxiolytic behavior (open field test and marble burying) in healthy mice, and the Scop-induced memory impairment (object recognition test and Morris water maze (MWM)) were assessed, and the biochemical parameters (malondialdehyde (MDA) and AChE levels) were measured after euthanasia. The subacute toxicological impact of MEDF was assessed in female Swiss mice for 28 days. MEDF and AFDF were available for the DPPH, ABTS and β-carotene/linoleic acid models. The results revealed that MEDF and AFDF exhibit anxiolytic effects and significantly alleviated Sco-induced memory impairment, inhibited AChE in the cortex (40%) and MDA (51.51%) levels. Reticuline was reported in AFDF and molecular coupling with AChE involves link-type hydrogen bonds and van der Waals interactions. MEDF exhibited antioxidant capacity (DPPH, IC50 = 18.10 ± 1.70 µg/mL; ABTS, IC50 = 10.41 ± 1.69 µg/mL). MEDF did not reveal signs of toxicity. In conclusion, D. furfuracea shows promise in mitigating scopolamine-induced memory deficits, potentially because it inhibits AChE activity, reduces MDA levels, and enhances antioxidant activities.

Green Radish Polysaccharide Prevents Alcoholic Liver Injury by Interfering with Intestinal Bacteria and Short-Chain Fatty Acids in Mice.

This study aimed to ascertain the potential benefits of green radish polysaccharide (GRP) in treating alcoholic liver disease (ALD) in mice and explore its mechanism of action. Using biochemical analysis, high-throughput sequencing of gut microbiota, and gas chromatography-mass spectrometry to measure short-chain fatty acids (SCFAs) in feces, we found that GRP intervention significantly improved lipid metabolism and hepatic function in mice subjected to excessive alcohol intake. The GRP intervention reduced malondialdehyde levels by 66% and increased total superoxide dismutase levels by 22%, thereby mitigating alcohol-induced oxidative stress. Furthermore, GRP intervention in mice with alcohol consumption resulted in a reduction in tumor necrosis factor, interleukin 6, and lipopolysaccharide levels by 12%, 9%, and 25%, respectively, effectively attenuating alcoholic liver inflammation. 16S rRNA amplicon sequencing demonstrated that excessive alcohol consumption markedly altered the gut microbiota composition in mice. The GRP treatment resulted in a significant reduction in the number of beneficial bacteria (Lactobacillus and Lachnospiraceae_NK4A136_group) and an increase in the proportion of harmful bacteria (Muribaculaceae and Verrucomicrobiota). The metabolomic analyses of the SCFAs demonstrated an increase in the contents of SCFAs, acetic acid, propionic acid, and butyric acid, following GRP supplementation. Furthermore, the metabolic levels of cholinergic synapses and glycolysis/gluconeogenesis were found to be modulated. In conclusion, these findings suggest that GRP may attenuate alcohol-induced oxidative damage in the liver by modulating the gut microbiota and hepatic metabolic pathways. This may position GRP as a potential functional component for ALD prevention.

Vancomycin-induced acute kidney injury in rats: beneficial effects deriving from the co-administration of tadalafil or vitamin C

Background: Vancomycin (VAN) is widely employed in clinical settings for the treatment of severe infections attributable to multi-resistant bacteria, with a notable emphasis on Gram-positive strains. However, its therapeutic usage is significantly hampered by the potential for acute kidney injury (AKI); specifically, the nephrotoxic effects it can exert. In controlled experimental studies, the intraperitoneal (i.p.) administration of VAN at a dose of 400 mg/kg/day over a consecutive seven-day period has been shown to induce considerable AKI in rats. In addressing this concern, vitamin C (VC), recognized traditionally for its antioxidant properties, has been utilized to mitigate various forms of organ damage and inflammatory responses, primarily by diminishing oxidative stress and attenuating inflammation. Concurrently, tadalafil (TAD), a phosphodiesterase type-5 inhibitor commonly associated with the treatment of erectile dysfunction, is also noted for its beneficial anti-inflammatory and antioxidant effects, alongside vasodilatory properties. Aim: This study was designed in order to investigate the protective capabilities of TAD or VC against the VAN-induced nephrotoxic effects in a rat model of AKI. Methodology: A total of 24 male albino Wistar rats were allocated into four distinct groups for the purpose of comparative analysis. Following treatment, both kidney and blood samples were obtained for further evaluation. The study randomized the rats into four categories: group 1 served as the control group, group 2 received VAN at a dosage of 400 mg/kg (via an i.p. injection on a daily basis), group 3 was administered VAN (400 mg/kg; i.p., daily) in conjunction with TAD at a dose of 5 mg/kg daily, and group 4 was given VAN (400 mg/kg; i.p., daily) alongside VC at a dosage of 200 mg/kg daily. Results: Evaluations included the measurement of renal function indicators such as the serum levels of urea, of creatinine, and the creatinine clearance. Additionally, the analysis involved the assessment of antioxidant markers in renal tissue homogenates, including malondialdehyde (MDA), glutathione, and superoxide dismutase. To further elucidate the inflammatory response, indices such as the levels of the tumor necrosis factor-alpha, the interleukins (IL)-1β and IL-6, and the nuclear factor-κB were evaluated within the renal tissue homogenate samples of the studied rats. The administration of VAN resulted in a marked elevation of renal biomarkers and inflammatory indices, alongside a significant reduction in antioxidant levels, with an exception for MDA (which exhibited increased levels); these changes reached statistical significance (p&lt;0.05) when measured against the control group. In contrast, the supplementation with TAD or VC was effective in significantly lowering the serum levels of both renal and inflammatory indicators when compared to the group treated solely with VAN. Furthermore, antioxidant measurements improved, alongside a reduction in MDA levels, thereby demonstrating significant differences (p&lt;0.05). Conclusion: The findings of this study reveal that both TAD and VC provide a degree of renal protection against the VAN-induced damage by enhancing kidney function markers and, concurrently, lessening oxidative stress and inflammatory responses associated with nephrotoxicity.

Deciphering the Impact of miR-92a-3p on Controlling BCL11A Gene Expression and Its Implication in Easing Thalassemia Symptoms

Deciphering the Impact of miR-92a-3p on Controlling BCL11A Gene Expression and Its Implication in Easing Thalassemia Symptoms

The impact of N-acetylcysteine on hypoxia-induced testicular apoptosis in male rats: TUNEL and IHC findings

The present study aimed to evaluate the impact of N-acetylcysteine (NAC) on testicular hypoxia caused by varicocele, focusing specifically on the regulation of genes related to apoptosis and oxidative stress in the testes of mature Wistar rats.Thirty-two rats were divided into four groups: Control (Sham), hypoxia, testicular hypoxia treated with NAC (Hypoxia + NAC), and healthy animals treated with NAC. After the 8-week treatment period, testicular histopathology and the levels of oxidative stress markers—superoxide dismutase (SOD), glutathione peroxidase (GPx), and malondialdehyde (MDA)—in serum were examined. The expression of Bax and Bcl-2 mRNA was analyzed using immunocytochemistry and RT-qPCR assays, while the apoptosis rate was determined using the TUNEL method.Histopathological evaluations showed that parameters such as Johnsen's score, epithelium width, and seminiferous tubule diameter indicated significant improvement in the Hypoxia + NAC group compared to the Hypoxia group. NAC administration resulted in elevated serum levels of GPx and SOD, accompanied by a reduction in MDA levels (p < 0.003). Furthermore, the study revealed that NAC decreased Bax expression and enhanced Bcl-2 gene and protein expression compared to the varicocele group (p < 0.05). Additionally, NAC administration significantly decreased the rate of apoptosis in germ cells (p < 0.05).These findings suggest that NAC administration can mitigate testicular damage induced by hypoxia from varicocele in rats, primarily due to its antioxidant properties.

Nano-herbal combination targets apoptosis in oral squamous cell carcinoma

BackgroundThis study evaluates the synergistic effects of the nano-herbal combination of Bischofia javanica leaves and Phaleria macrocarpa fruits on oral squamous cell carcinoma (OSCC), a metastatic keratinocyte malignancy. Materials and MethodsThirty-six male Wistar rats were divided into six groups: a negative control group (K0), an OSCC-induced control group (K1), and four treatment groups (P1-P4), which received oral treatments for four weeks. Blood samples were analyzed for superoxide dismutase (SOD), malondialdehyde (MDA), and vascular endothelial growth factor (VEGF) levels, while immunohistochemistry was used to assess the expression of heat shock protein 70 (HSP-70), caspase-3, caspase-8, caspase-10, and MDA. ResultsNano-herbal treatment significantly reduced MDA levels, particularly in groups P3 and P4, with reductions comparable to those seen in the Vitamin C treatment group (p < 0.05). SOD levels were highest in group P3 (p < 0.05), similar to the negative control group (K0), while VEGF levels were significantly elevated in the positive control group (K1) (p < 0.05). Group P3 exhibited the highest expression of HSP-70 (p < 0.05). Caspase-3 expression was significantly higher in P3 compared to K1 (p = 0.0007), while caspase-8 and caspase-10 expressions were also significantly elevated in groups P2, P3, and P4 (p < 0.05). Group P3 showed the most pronounced effects in promoting apoptosis, with caspase-3 and caspase-8 levels markedly higher compared to K1. ConclusionThe combined nano-herbal formulations of Bischofia javanica leaves and Phaleria macrocarpa fruits effectively mitigates oxidative stress, induces apoptosis, and holds potential as a therapeutic approach for managing oral squamous cell carcinoma (OSCC).

Improving maize yield and drought tolerance in field conditions through activated biochar application

Amidst depleting water resources, rising crop water needs, changing climates, and soil fertility decline from inorganic modifications of soil, the need for sustainable agricultural solutions has been more pressing. The experimental work aimed to inspect the potential of organically activated biochar in improving soil physicochemical and nutrient status as well as improving biochemical and physiological processes, and optimizing yield-related attributes under optimal and deficit irrigation conditions. Biochar enhances soil structure, water retention, and nutrient availability, while improving plant nutrient uptake and drought resilience. The field experiment with maize crop was conducted in Hardaas Pur (32°38.37’N, 74°9.00’E), Gujrat, Pakistan. The experiment involved the use of DK-9108, DK-6321, and Sarhaab maize hybrid seeds, with five moisture levels of evapotranspiration (100% ETC, 80% ETC, 70% ETC, 60% ETC, and 50% ETC) maintained throughout the crop seasons. Furthermore, activated biochar was applied at three levels: 0 tons/ha (no biochar), 5 tons per hectare, and 10 tons per hectare. The study’s findings revealed significant improvements in soil organic matter, bulk density, nutrient profile and total porosity with biochar supplementation in soil. Maize plants grown under lower levels of ETC in biochar supplemented soil had enhanced membrane stability index (1.6 times higher) increased protein content (1.4 times higher), reduced malondialdehyde levels (0.7 times lower), improved antioxidant enzyme activity (1.3 times more SOD and POD activity, and 1.2 times more CAT activity), improved relative growth (1.05 times more) and enhanced yield parameters (26% more grain and stover yield, 16% more 1000-seed weight, 29% more total seed weight, 33% more apparent water productivity) than control. Additionally, among the two biochar application levels tested, the 5 tons/ha dose demonstrated superior efficiency compared to the 10 tons/ha biochar dose.

Application of Fluorescent Composite Materials as a Sustained Release System in Treatment of Polycystic Ovary Syndrome.

Polycystic ovary syndrome (PCOS) is a multifactorial disease characterized by oxidative stress and follicular dysfunction, leading to menstrual irregularities, hyperandrogenism, and infertility. Traditional drug delivery methods often result in drug loss and side effects on normal tissues. To address these issues, we synthesized two novel Co(II)-containing coordination polymers (CPs), {[Co(L)(H2O)2]·2H2O}n (1) and {[Co(L)(H2O)2]·1.5H2O}n (2), through the reaction of the T-shaped ligand (4 - 3'-pyridyl-,6 - 4'-carboxylphenyl)picolinic acid (H2L) with Co(NO3)2·6H2O via a solvothermal process. Fluorescence spectroscopy revealed that the fluorescence emission of the CPs originates from the ligand, indicating their potential application as blue fluorescence materials. Subsequently, we encapsulated these CPs with hyaluronic acid (HA) and carboxymethyl chitosan (CMCS) hydrogels to create two types of metal gel particles carrying spironolactone (HA/CMCS-CPs@spironolactone). SEM and TEM analyses showed that the material consists of tightly stacked sheet-like structures with an average size of approximately 100nm. Thermogravimetric analysis (TGA) indicated that the material begins to decompose at around 115°C, demonstrating good thermal stability. We assessed the inhibitory effects of these materials on oxidative stress induced by PCOS. The results showed that both types of spironolactone-loaded metal gel particles significantly reduced malondialdehyde (MDA) levels, particularly the particles constructed with CP2. HA/CMCS-CP1@spironolactone reduced MDA levels by approximately 17% and 46% at low and high concentrations, respectively, while HA/CMCS-CP2@spironolactone decreased MDA levels by about 55% and 39% at high and low concentrations, respectively. Therefore, the novel drug delivery system reported in this study has the potential to become a safe and effective option for the localized treatment of PCOS.

Effect of (R)-(−)-Linalool on endothelial damage: Sex differences

Oxidative stress and inflammation are responsible for endothelial damage displaying many sex differences. Lipopolysaccharide (LPS) is a pathogenic stimulus that can trigger inflammation, contributing to endothelial dysfunction. Given the scientific evidence on the effectiveness of herbal extracts in managing endothelial dysfunction, we considered the (R)-(−)-Linalool (LIN), an aromatic monoterpene alcohol, as a bioactive phytochemical compound that could prevent and improve endothelial injury. In this study, we evaluated the effect of the LIN on LPS-induced damage in female and male human umbilical vein endothelial cells (FHUVECs and MHUVECs), measuring cell viability, cytokines release (IL-6 and TNF-α), malondialdehyde (MDA), and nitrites.LPS significantly reduced viability both in MHUVECs and FHUVECs. Moreover, LPS increased the IL-6, TNF-α, and MDA level only in FHUVECs if compared to basal value; despite that, LPS reduced nitrites only in MHUVECs. LIN alone did not affect the parameters measured except for an increase in nitrites in FHUVECs. Nevertheless, LIN reduced damage and restored endothelium viability reduced by LPS without a clear sex difference. Under LPS, LIN inhibited IL-6 release and reduced MDA levels only in FHUVECs.The present data confirm the existence of sex differences in the behavior of HUVECs under LPS conditions. The administration of LIN seems to have a more evident effect on FHUVECs after damage induced by LPS. These LIN effects are important to conduct further well-designed studies on the sex-specific use of this compound on vascular endothelial injury.

Continuous Theta Burst Stimulation Inhibits Oxidative Stress-Induced Inflammation and Autophagy in Hippocampal Neurons by Activating Glutathione Synthesis Pathway, Improving Cognitive Impairment in Sleep-Deprived Mice.

Sleep deprivation (SD) has been reported to have a negative impact on cognitive function. Continuous theta burst stimulation (cTBS) shows certain effects in improving sleep and neurological diseases, and its molecular or cellular role in SD-induced cognition impairment still need further exploration. In this study, C57BL/6 mice were subjected to 48h of SD and cTBS treatment, and cTBS treatment significantly improved SD-triggered impairment of spatial learning and memory abilities in mice. Additionally, cTBS reduced malondialdehyde levels, increased superoxide dismutase activities, and inhibited the production of inflammatory cytokines, alleviating oxidative stress and inflammation levels in hippocampal tissues of SD model mice. cTBS decreased LC3II/LC3I ratio, Beclin1 protein levels, and LC3B puncta intensity, and elevated p62 protein levels to suppress excessive autophagy in hippocampal tissues of SD-stimulated mice. Then, we proved that inhibiting oxidative stress alleviated inflammation, autophagy, and death of hippocampal neuron cells through an in vitro cellular model for oxidative stress, and cTBS treatment promoted the production of glutathione (GSH), the nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf2) and the mRNA expression of GSH synthesis-related genes to enhance antioxidant capacity in hippocampal tissues of SD mice. An Nrf2 inhibitor ML385 or a GSH synthesis inhibitor BSO reversed the alleviating effects of cTBS treatment on oxidative stress-associated damage of hippocampal tissues and cognitive impairment in SD model mice. Altogether, our study demonstrated that cTBS mitigates oxidative stress-associated inflammation and autophagy through activating the Nrf2-mediated GSH synthesis pathway, improving cognitive impairment in SD mice.

Exploiting the synergistic influence of AgNPs-TiO2NPs: enhancing phytostabilization of pb and mitigating its toxicity in Vigna unguiculata

In this study, a composite of silver and titanium dioxide nanoparticles (AgNPs-TiO2NPs) was examined for its synergistic effects on phytostabilization of lead (Pb) and mitigation of toxicity in cowpea (Vigna unguiculata (L) Walp). Seeds of V. unguiculata were wetted with water, 0.05 and 0.1 mgL−1 Pb and 25 mgmL−1 each of AgNPs, TiO2NPs, and AgNPs-TiO2NPs. Root lengths of V. unguiculata were reduced by 25% and 44% at 0.05 and 0.1 mgL−1 Pb, respectively, while shoot lengths were reduced by 2% and 7%. In V. unguiculata, AgNPs and TiO2NPs significantly improved physiological indicators and mitigated Pb effects, with TiO2NPs modulating physiological parameters more effectively than AgNPs. The composite (AgNPs-TiO2NPs) synergistically regulated V. unguiculata physiology better than individual nanoparticles. Compared to individual AgNPs and TiO2NPs, the composite (AgNPs-TiO2NPs) synergistically increased antioxidant activity by 12% and 9%, and carotenoid contents by 88%. Additionally, AgNPs-TiO2NPs effectively reduced malondialdehyde levels by 29%, thereby mitigating the effects of Pb on V. unguiculata better than individual nanoparticles. AgNPs-TiO2NPs enhanced Pb immobilization by 57%, reducing its translocation from soil to shoots compared to V. unguiculata wetted with water. The bioconcentration and translocation factors of Pb indicate that phytostabilization was most effective when the composite was used.

Carbon monoxide refines ovarian structure changes and attenuates oxidative stress via modulating of heme oxygenase system in a rat model of polycystic ovary syndrome: An experimental study.

Carbon monoxide (CO), influences ovarian function, pregnancy, and placental health. Heme oxygenase (HO)-1 and its products, including CO, exhibit protective and anti-inflammatory properties. This study investigates the protective effects of CO released by the carbon dioxide-releasing molecule (CORM)-2 against oxidative stress, functional and structural changes of the ovaries, and HO-1 expressions in female rats suffering from polycystic ovary syndrome (PCOS). In this experimental study, 24 Rattus norvegicus var. Albinus female rats (180-200 gr, 8 wk) were randomly divided into 4 groups (n = 6/each): control, CORM-2 (10 mg/kg), PCOS (induced by 4 mg/kg, intramuscular injection and a single dose of estradiol valerate), PCOS + CORM-2. Ovary histological changes were evaluated by crystal violet staining. Malondialdehyde (MDA) level and superoxide dismutase (SOD) activity of ovarian tissue were assessed using enzyme-linked immunosorbent assay. HO-1 expression was evaluated using Western blot. Corpus luteal formation significantly decreased in the PCOS group and was significantly restored with CORM-2 administration compared to the control group (p 0.05). The expression of ovarian HO-1 protein was reduced in the PCOS group compared to controls (p 0.01), and administration of CORM in PCOS rats significantly increased its expression (p 0.0001). In addition, CORM administration markedly reduced ovarian MDA levels and restored SOD activity (p 0.0001). CORM-2 administration to PCOS rats created protective effects by reducing oxidative stress (reducing MDA level and restoring SOD activity) and increasing ovarian HO-1 protein.

Acetyl-11-keto-beta-boswellic Acid (AKBA) Ameliorates Histopathological and Oxidative Stress Brain Disorders in the Experimental Model of Cuprizone-induced Demyelination

Background: Multiple sclerosis is an inflammatory autoimmune disease that destroys the myelin sheath (demyelination) and neurons of the central nervous system (CNS), with several factors contributing to its development. Objectives: This study investigated the impact of administering acetyl 11-keto-beta boswellic acid (AKBA) in an animal model of cuprizone-induced demyelination. Methods: Thirty-two C57BL/6 female mice (WT; 20 - 25 g, 8 - 10 weeks old) were randomly divided into four groups: (1) Control (CO), (2) Cuprizone-treated (CPZ, 0.3%), (3) AKBA + CPZ (50 mg/kg, pre-treatment), and (4) CPZ + AKBA (50 mg/kg, post-treatment). At the end of the study, the weight and movement balance of the animals were assessed using the rotarod test. After sampling the brain, the extent of the demyelination area in the corpus callosum was measured with Luxol Fast Blue staining, the number of oligodendrocyte cells was determined by immunohistochemistry, and the levels of malondialdehyde (MDA) and total antioxidant capacity (TAC) as oxidative stress factors in the brain tissue were measured. Results: The use of acetyl-11-keto-beta boswellic acid in the cuprizone-induced demyelination model prevented weight loss, increased animal resistance in the rotarod test, and reduced the demyelination rate in the corpus callosum. Additionally, AKBA elevated the number of oligodendrocytes and total antioxidant capacity levels while reducing malondialdehyde levels in brain tissue. Pretreatment with AKBA exhibited a more significant effect. Conclusions: This study showed that acetyl-11-keto-beta boswellic acid, an important active substance of the frankincense plant, likely protects the nervous system and neurons in the cuprizone-induced demyelination model by preventing the decrease in the number of oligodendrocytes, reducing oxidative stress, and preventing the destruction of myelin.

Potency of Red Guava Fruitghurt as an Antioxidant

Antioxidants are compounds needed by the body to protect body cells from damage caused by free radicals. Free radicals can damage macromolecules such as cell membrane lipids, DNA, and proteins, and cause cell oxidative stress. Red Guava Fruitghurt is a probiotic with the basic ingredients of fruit. The purpose of this study is to determine the effect of fruitghurt on the MDA levels in the blood of mice induced by CCl4. This was an experimental study conducted using mice (Mus musculus) which were divided into three treatment groups – the negative control group (that were not induced and were not given fruitghurt), the positive control group (that were induced with CCl4 but not given fruitghurt), and the treatment group (that were induced with CCl4 and given fruitghurt). The data obtained in the study were analyzed using the one-way ANOVA test, and the results showed significant differences between the treatment groups. Giving fruitghurt to the P1 group had a significant effect on reducing malondialdehyde levels compared to the positive control group, with a P-value of 0.03. Lactobacillus acidophilus bacteria found in this fruitghurt can reduce oxidative stress by inhibiting ascorbic acid oxidation, reducing activity and capturing radial superoxide anions, hydrogen peroxide, and free radicals, and inhibiting lipid oxidation. So this fruitghurt has antioxidant properties. This is also strengthened by the content of red guava which is rich in phenolics, flavonoids, and vitamin C that have good free radical scavenging activity, so guava fruit is a natural source of antioxidants. Keywords: antioxidants, fruitghurt, malon dialdehyde, probiotics, red guava

Effects of Yogic Practices Synchronized With Bandha and Kumbhaka on Biological and Psychological Factors of Aging in COVID-19-Recovered Patients: A Randomized Controlled Trial.

Background and objectives Accelerated biological aging and age-associated diseases are strong risk factors for mortality and morbidity. Oxidative stress (OS) and anemia are possible pathophysiological causes of the various organ dysfunctions observed during COVID-19, decreasing health and life span.Ancient Yogic science seems to optimize all dimensions of human existence. As mentioned in ancient Yogic scriptures and documented in various studies, Yoga has been found to control accelerated biological aging and associated diseases. The study's objective was to authenticate and look into the effect of Yogic practices specifically synchronized with Kumbhaka and Bandha on markers of accelerated aging. Methods This randomized controlled trial was carried out in Mahendergarh city of Haryana on COVID-19-recovered adults aged between 30 and 60 years; 126 adults were randomized into two groups from Mahendergarh city: a control group (CG), 61 adults, and the experimental group (EG), 65 adults. During the final analysis, 56 adults in the experimental groupreceived Yogic intervention for 120 days, and 61 adults remained the same in the control group during the intervention period. Consenting participants were randomized using computer-generated block randomization. The Yogic intervention was done 60 minutes/day five days a week for six months. Both groups' laboratory tests were carried out, which included malondialdehyde (MDA) level, total antioxidant capacity (TAC), glutathione (GSH) levels, hemoglobin (Hgb) level, body mass index (BMI), mental stress (perceived stress), and quality of life (QOL), which were estimated before and after the Yogic intervention. Results Yoga practice for 120 days (three mandals) in the experimental group has significantly reduced MDA level (p = 0.03) and perceived stress level (Perceived StressScale {PSS}) (p = 0.047), and BMI decreased in the Yoga group from 24.2 ± 4.8 to 23.6 ± 4.8, but no significant difference was observed in the values of BMI (p = 0.54).Improved antioxidant levels such as GSH level (p = 0.02), serum ferric-reducing antioxidant power (FRAP)/TAC activity (p = 0.04), and Hgb level (p = 0.02) were reported; with this, improved quality of life, World Health Organization Quality of Life (WHOQOL) Physical (p = 0.03), WHOQOL Psychological (p = 0.02), WHOQOL Social (p = 0.04), and WHOQOL Environment (p = 0.006), has been observed in the experimental group, whereas in the control group, we observed no significant difference in MDA level (p = 0.38), GSH level (p = 0.97), TAC level (p = 0.96), Hgb level (p = 1), BMI (p = 0.85), PSS (p = 0.83), and quality of life, WHOQOL Physical (p = 0.37), WHOQOL Psychological (p = 0.88), WHOQOL Social (p = 0.96), and WHOQOL Environment (p = 0.32). Conclusion These findings suggest that Yoga synchronized with Kumbhaka and Bandha may be a useful strategy for lowering oxidative stressand mental stressand improving antioxidant defense, hemoglobin level, and overall quality of life in COVID-19-recovered people, which might help reverse the biological decline of the human body and mind.The results of this study show that Yoga may break the link between old age and ill health. Hence, Yoga (with Bandha and Kumbhaka)may bethe most reproducible way to extend the life span of humans, as mentioned in ancient Yogic scriptures.

Substituting ethoxyquin with tea polyphenols and propyl gallate enhanced feed oxidative stability, broiler hepatic antioxidant capacity and gut health

The safety of ethoxyquin has garnered increasing attention. This study evaluated the effects of partially substituting ethoxyquin with tea polyphenols and propyl gallate on feed oxidative stability, hepatic antioxidant properties, intestinal morphology and barrier functions, as well as the antioxidant and anti-inflammatory profiles of the intestinal mucosa in broilers. A total of 351 one-day-old male Arbor Acres Plus broilers were randomly assigned to 3 groups, each comprising 9 replicates with 13 birds per replicate. The treatments included a control group (CON) fed a basal diet, an ethoxyquin group (EQ) that received the basal diet supplemented with 120 g/t of ethoxyquin, and a substitution group (TP) receiving the basal diet supplemented with 6 g/t of tea polyphenols, 6 g/t of propyl gallate, and 30 g/t of ethoxyquin. In vitro experiments showed that both EQ and TP supplementation significantly reduced the acid value (AV), peroxide value (POV), and total oxidation value (TOV) of the feeds, with the TP group exhibiting lower AV and TOV than the EQ group. In vivo assessments revealed no significant differences in growth performance among the groups. Additionally, the TP group exhibited significantly higher glutathione peroxidase activity, increased glutathione content, and elevated protein expression of Keap1, Nrf2, and NQO1 in the liver compared to the control group (P < 0.05). Moreover, dietary TP significantly increased liver catalase activity, glutathione content, and NQO1 protein levels compared to the EQ group (P < 0.05). Both additives effectively reduced malondialdehyde levels in the intestinal mucosa by approximately 50% (P < 0.05) through the activation of the Nrf2/ARE pathway, as indicated by increased mRNA expression of TXN, CAT, GPX1, and GPX4 (P < 0.05). Furthermore, compared to the control group, the TP group exhibited greater villus height and villus height-to-crypt depth ratio (VCR) in the jejunum, as well as elevated VCR in the ileum (P < 0.05). The TP group also achieved the lowest serum levels of diamine oxidase activity, D-lactate and lipopolysaccharide contents among all groups (P < 0.05). The inclusion of both EQ and TP increased the mRNA expression of Occludin, Claudin-1, Mucin-2, and E-cadherin in the jejunum (P < 0.05). Moreover, the combination of tea polyphenols and propyl gallate effectively mitigated the proinflammatory effect of ethoxyquin, as evidenced by reductions in TNF-α, IL-18, and IFN-γ expression, potentially mediated by inhibition of the TLR-4/MyD88/NF-κB signaling pathway. In conclusion, this study demonstrates that partially replacing ethoxyquin with tea polyphenols and propyl gallate enhances feed oxidative stability, liver antioxidant capacity, and gut health in broilers, suggesting an efficient alternative with a lower dosage requirement.