Steady glacier runoff is related to the security and resilience of water resources in meltwater recharge basins, so the status and change of glaciers and their response to climate change in the upper reaches have received widespread concerns. Here, the spatiotemporal characteristics of glacier wastage in the Upper Reaches of Shule River Basin (URSRB) driven by climate change were analyzed based on multi-source and multi-temporal remotely sensed images. Firstly, we extracted multi-temporal glacier outlines from the Landsat time series data using Google Earth Engine (GEE) for seven different periods every approximately 5years from 1990 to 2020. The spatiotemporal analysis of URSRB glaciers demonstrates a sustained reduction in glacier area from 481.07 ± 24.24 km2 in 1990 to 384.05 ± 22.71 km2 in 2020, corresponding to a glacier shrinkage rate of - 0.67 ± 0.23%/year, characterized by considerable temporal variability. Secondly, multi-temporal DEMs derived from ASTER stereo imagery spanning from 2000 to 2020 were used to compute the glacier surface elevation changes and determine the glacier mass loss. The overall glacier surface elevation change rate was - 0.32 ± 0.14m/year, equivalent to a mass balance of - 0.28 ± 0.12m w.e./year. Lastly, to better apprehend the long-term response of URSRB glaciers to climate change, studies on climate change were carried out based on the EAR5-Land reanalysis dataset. The long-term trend of glacier wastage is attributed to the increase in summer temperature, and the negative effects of increased summer temperature on glaciers exceeded the positive effects of increased annual precipitation. In summary, glaciers in the URSRB have experienced a significant area reduction and accelerated mass loss against the backdrop of climatic warming and humidification.