Reduce Plant Diseases Research Articles

Rhizosphere microbial community assembly as influenced by reductive soil disinfestation to resist successive cropping obstacle.

Reductive soil disinfestation (RSD), which involves creating anaerobic conditions and incorporating large amounts of organic materials into the soil, has been identified as a reliable strategy for reducing soilborne diseases in successive cropping systems. However, limited research exists on the connections between soil microorganism composition and plant diseases under various types of organic material applications. This study aimed to evaluate the effects of distinct RSD strategies (control without soil amendment; RSD with 1500 kg ha-1 molasses powder; RSD with 3000 kg ha-1 molasses powder; RSD with 3000 kg ha-1 molasses powder and 37.5-41.3 kg ha-1 microbial agent) on the plant disease index, bacterial community composition and network structure in rhizosphere soil. RSD treatments significantly reduced the occurrence of black shank disease in tobacco and increased soil bacterial diversity. High amounts of molasses powder in RSD treatments further enhanced disease inhibition and reduced fungal abundance and Shannon index. RSD also increased the relative abundance of bacterial phylum Firmicutes and fungal phylum Ascomycota, while decreasing the relative abundance of bacterial phyla Chloroflexi and Acidobacteriota and fungal phylum Basidiomycota in rhizosphere soil. A multiple regression model identified bacterial positive cohesion as the primary factor influencing the plant disease index, with a greater impact than bacterial negative cohesion and community stability. The competition among beneficial bacteria for creating a healthy rhizosphere environment is likely a key factor in the success of RSD in reducing plant disease risk. RSD, especially with higher rates of molasses powder, is a viable strategy for controlling black shank disease in tobacco and promoting soil health by fostering beneficial microbial communities. This study provides guidelines for soil management and plant disease prevention. © 2024 Society of Chemical Industry.

Research progress of Bacillus velezensis in plant disease resistance and growth promotion

Bacillus velezensis has a wide range of beneficial activities, such as fighting plant diseases, promoting growth, improving crops’ ability to handle stress, and boosting plant defenses. These traits make it a strong candidate for agricultural use, especially as a biocontrol agent and growth-promoting bacteria. This review takes a close look at the origins, working mechanisms, and potential agricultural benefits of B. velezensis. Both lab and field studies have shown its success in reducing plant diseases and supporting crop growth by producing helpful compounds, taking up space in the environment, and strengthening plant defenses. Although B. velezensis is already used in some fertilizers and biopesticides, there are still challenges in scaling up production, selecting the right strains, and ensuring product stability. This review points out current research gaps and suggests future directions, such as improving strain selection, developing better biofertilizers, and advancing production techniques to make the most of B. velezensis in agriculture. These findings aim to guide further research and improve its use in sustainable farming.

RNA interference: a promising biotechnological approach to combat plant pathogens, mechanism and future prospects.

Plant pathogens and other biological pests represent significant obstacles to crop Protection worldwide. Even though there are many effective conventional methods for controlling plant diseases, new methods that are also effective, environmentally safe, and cost-effective are required. While plant breeding has traditionally been used to manipulate the plant genome to develop resistant cultivars for controlling plant diseases, the emergence of genetic engineering has introduced a completely new approach to render plants resistant to bacteria, nematodes, fungi, and viruses. The RNA interference (RNAi) approach has recently emerged as a potentially useful tool for mitigating the inherent risks associated with the development of conventional transgenics. These risks include the use of specific transgenes, gene control sequences, or marker genes. Utilizing RNAi to silence certain genes is a promising solution to this dilemma as disease-resistant transgenic plants can be generated within a legislative structure. Recent investigations have shown that using target double stranded RNAs via an effective vector system can produce significant silencing effects. Both dsRNA-containing crop sprays and transgenic plants carrying RNAi vectors have proven effective in controlling plant diseases that threaten commercially significant crop species. This article discusses the methods and applications of the most recent RNAi technology for reducing plant diseases to ensure sustainable agricultural yields.

Design, Synthesis, and Bioactivity Determination of Novel Malononitrile Derivatives Containing 1,2,3-Triazole.

Using antifungal agrochemicals as the most economical solution might reduce plant diseases caused by pathogenic fungi, which have a significant negative impact on the quality and yield of food worldwide. In this work, 33 compounds (G) containing 1,2,3-triazole and malononitrile structures were synthesized. When the compounds were tested in vitro against six fungal species, they exhibited significant fungicidal activity toward Botrytis cinerea and Rhizoctonia solani. Compounds G17 and G30 displayed promising in vivo efficacy, with an EC50 of 0.19 and 0.27 mg/L respectively against R. solani. Fungal ergosterol production was suppressed by compounds G17 and G30, according to a preliminary analysis of their mechanism of action on R. solani using transcriptomics and scanning electron microscopy. It has been shown through experimentation that compounds G17 and G30 prevent R. solani from synthesizing ergosterol. Ultimately, it was anticipated that compounds G17 and G30 would be discovered to be low-toxic.

ХИМИЯЛЫК ЖЕР СЕМИРТКИЧТЕРДИ АЙЫЛ ЧАРБАСЫНДА ПАЙДАЛАНУУНУН КООПСУЗДУГУ

This article talks about chemical fertilizers and provides safety precautions when using them. It also shows the classification of fertilizers, types, safety measures and negative consequences, as well as ways to solve them. Today it is noted that research is being conducted on the impact of chemicalization on agriculture, the environment and human health. Chemical fertilizers are widely used in agriculture to increase crop yields. They provide nutrients to plants, improve soil structure, and help reduce plant diseases and pests. However, improper and excessive use of chemical fertilizers can have negative impacts on the environment and human health. This article discusses the safe use of chemical fertilizers and their effects.

Aerobic Composting of Organic Waste, Alternative and an Efficient Solid Waste Management Solution

The process of composting which involves the treatment of organic wastes to obtain a stable clean product, has become an increasingly desirable choice, at any scale from home to large waste treatment plants. Composting serves as an alternative to landfilling for managing biodegradable waste while also increasing or preserving soil organic matter, decreasing solid waste, and reducing disposal costs. The compost product improves the condition of the soil, reduces erosion, and helps reduce plant diseases without having adverse impacts on the environment. This study aims to manufacture high-quality compost as a solid waste management method by converting organic waste into a useful resource for the community in a financially and ecologically sustainable manner. We investigated the potential of aerobic composting by controlling the operation process parameters, including monitoring the pH value, temperature, electrical conductivity (EC), carbon-to-nitrogen ratio (C/N), and moisture content during the composting. We studied the physiochemical and biological changes of composting using a 60-liter bioreactor. The composting process determined an average pH value between 6.9 and 7.8, and it revealed a decrease in TOC from 26.18 to 22.19. Additionally, compost process monitoring indicated a maximum temperature rise of 69 °C over the first three days, and then a decrease after 20 days of air temperature. Total nitrogen in the NH4+/NO3-ratio nitrification decreased to less than 1.4, and the compost in the C/N ratio decreased from 20.75 to 10.035 and setting quality criteria by comparing the final product quality produced through the in-vessel composting.

Bacillus‐based product protects soybean, improves grain yield and produces metabolites able to promote plant growth and antagonize pathogenic fungi

AbstractSoybean is frequently affected by pathogenic fungi, which are usually managed by chemicals. Foliar sprays with biological agents have been introduced as new allies to reduce plant diseases. This study verified foliar sprays of Bacillus subtilis‐based product applied for three consecutive cropping seasons associated or not with chemical fungicides to control Asian rust (Phakopsora pachyrhizi) and the effect of B. subtilis‐produced metabolites against pathogenic fungi and on soybean growth. Field treatments were as follows: (i) Foliar application of B. subtilis‐based product, followed by chemical sprays; (ii) chemical management alone; (iii) B. subtilis‐based product alone, and (iv) control treatment with water sprays. In the three years, B. subtilis‐based product alone or followed by chemicals reduced at least 75% the Asian rust severity, similar to chemical management. Moreover, soybean grain yield increased when B. subtilis‐based product was followed by chemicals. Cell‐free filtrates and volatile organic compounds (VOCs) from B. subtilis isolate or from the formulated bioproduct were tested against fungi pathogens Fusarium cf. incarnatum/equiseti and Macrophomina phaseolina. All filtrates and VOCs emission reduced more than 40% the mycelial growth of both fungi. Also, the filtrates stimulated the soybean growth at a rate similar to the bioproduct, indicating the potential of the B. subtilis compounds to control fungi pathogens and promote plant growth. Therefore, the B. subtilis‐based product showed a satisfactory protection and yield increment for soybean and B. subtilis‐produced metabolites antagonized pathogenic fungi and stimulated the soybean plant growth.

Responses of plant immune system and rhizosphere soil microbiome to the elicitor BAR11 in Arabidopsis thaliana

Microbial elicitors have been shown to boost plant immunity by inducing defense responses to reduce plant disease. However, little is known about the changes in plant microbiome and metabolism in the process of enhancing plant immunity with elicitors. The protein elicitor BAR11, from Saccharothrix yanglingensis Hhs.015, induces defense responses in Arabidopsis thaliana that enhances resistance to pathogens. In this study, bar11 was inserted into Col-0 A. thaliana to obtain BAR11-Trans plant by Agrobacterium-mediated immersion transformation. BAR11-Trans exhibited an elevated defense level against Pseudomonas syringae pv. tomato DC3000 while experiencing a decline in biomass production of above-ground parts. In the process, BAR11-Trans increased the activity of phenylalanine ammonia lyase (PAL) and catalase (CAT), and up-regulated genes related to plant defense pathways. Furthermore, BAR11-Trans decreased root tip reactive oxygen species (ROS) levels while increasing ROS burst in the leaves. Soil transplantation experiments showed that soil planted with BAR11-Trans could enhance the resistance of Col-0 A. thaliana to DC3000. Analysis of A. thaliana rhizosphere soil through 16S rRNA amplified sequencing revealed that BAR11-Trans increased the relative abundance and diversity of the rhizosphere microbial community, leading to the recruitment of more plant probiotics. Additionally, the accumulation of kaempferitrin and robinin in BAR11-Trans influenced the physicochemical properties of rhizosphere soil and the composition of the bacterial community. In summary, BAR11-Trans exhibited heightened defense levels compared to Col-0, leading to increased secretion of secondary metabolites and the recruitment of a greater number of microorganisms to adapt to the environment. These findings offer novel insights for the potential application of elicitors in agricultural disease control.

Nano-Agrochemicals as Substitutes for Pesticides: Prospects and Risks.

This review delves into the mesmerizing technology of nano-agrochemicals, specifically pesticides and herbicides, and their potential to aid in the achievement of UN SDG 17, which aims to reduce hunger and poverty globally. The global market for conventional pesticides and herbicides is expected to reach USD 82.9 billion by 2027, growing 2.7% annually, with North America, Europe, and the Asia-Pacific region being the biggest markets. However, the extensive use of chemical pesticides has proven adverse effects on human health as well as the ecosystem. Therefore, the efficacy, mechanisms, and environmental impacts of conventional pesticides require sustainable alternatives for effective pest management. Undoubtedly, nano-agrochemicals have the potential to completely transform agriculture by increasing crop yields with reduced environmental contamination. The present review discusses the effectiveness and environmental impact of nanopesticides as promising strategies for sustainable agriculture. It provides a concise overview of green nano-agrochemical synthesis and agricultural applications, and the efficacy of nano-agrochemicals against pests including insects and weeds. Nano-agrochemical pesticides are investigated due to their unique size and exceptional performance advantages over conventional ones. Here, we have focused on the environmental risks and current state of nano-agrochemicals, emphasizing the need for further investigations. The review also draws the attention of agriculturists and stakeholders to the current trends of nanomaterial use in agriculture especially for reducing plant diseases and pests. A discussion of the pros and cons of nano-agrochemicals is paramount for their application in sustainable agriculture.

Evaluating the intention to use Industry 5.0 (I5.0) drones for cleaner production in Sustainable Food Supply Chains: an emerging economy context

PurposeThe purpose of this study is to evaluate food supply chain stakeholders’ intention to use Industry 5.0 (I5.0) drones for cleaner production in food supply chains.Design/methodology/approachThe authors used a quantitative research design and collected data using an online survey administered to a sample of 264 food supply chain stakeholders in Nigeria. The partial least square structural equation model was conducted to assess the research’s hypothesised relationships.FindingsThe authors provide empirical evidence to support the contributions of I5.0 drones for cleaner production. The findings showed that food supply chain stakeholders are more concerned with the use of I5.0 drones in specific operations, such as reducing plant diseases, which invariably enhances cleaner production. However, there is less inclination to drone adoption if the aim was pollution reduction, predicting seasonal output and addressing workers’ health and safety challenges. The findings outline the need for awareness to promote the use of drones for addressing workers’ hazard challenges and knowledge transfer on the potentials of I5.0 in emerging economies.Originality/valueTo the best of the authors’ knowledge, this study is the first to address I5.0 drones’ adoption using a sustainability model. The authors contribute to existing literature by extending the sustainability model to identify the contributions of drone use in promoting cleaner production through addressing specific system operations. This study addresses the gap by augmenting a sustainability model, suggesting that technology adoption for sustainability is motivated by curbing challenges categorised as drivers and mediators.

Immune-enhancing miPEPs reduce plant diseases and offer new solutions in agriculture.

Immune-enhancing miPEPs reduce plant diseases and offer new solutions in agriculture.

Wheat and faba bean intercropping and cultivar impacts on morphology, disease, and yield

AbstractModern agriculture relies heavily on synthetic fertilizer and pesticide inputs. Pressure is increasing to find alternatives that reduce such inputs. In comparison to monocultures, intercropping can reduce plant diseases and increase yield, thereby reducing inputs and maximizing land use efficiency. However, knowledge gaps remain regarding which crop and cultivar combinations maximize such benefits. Here, we grew two wheat (Triticum aestivum L.) and six faba bean (Vicia faba L.) cultivars in monoculture and intercrops over three seasons and measured plant morphology, disease prevalence, and yield. Wheat ear development was slower in monocultures but varied by cultivar and year. Wheat cultivars senesced faster in monocultures versus certain faba bean intercrop combinations. In both wheat cultivars, Fusarium spp. severity was higher, while yellow rust (Puccinia striiformis f.sp. tritici Westend.) was lower in monocultures versus intercropped plots but varied by year and faba bean cultivar. Chocolate spot (Botrytis fabae Sardiña) in faba bean was higher in the cultivar ‘Louhi’ when grown in monoculture. Wheat cultivars yielded higher in monoculture versus intercropped plots. Faba bean yield was higher in monocultures but depended on wheat cultivar and year. Land equivalent ratios (LER) were not affected by interactions between cultivars or years but were always above one in intercropped plots. This indicates that it is always more efficient for yield to intercrop. Our results show that the benefits of intercropping with different wheat and faba bean cultivars varied, indicating that specific goals (i.e., disease suppression and yield) should be considered when selecting wheat–faba bean cultivar combinations.

Biocontrol Potential of Beneficial Fungus Aureobasidium pullulans Against Botrytis cinerea and Colletotrichum acutatum.

Biological control is a promising approach to reduce plant diseases caused by fungal pathogens and ensure high productivity in horticultural production. In the present study, we evaluated the biocontrol potential and underlying mechanisms of the beneficial fungus Aureobasidium pullulans against Botrytis cinerea and Colletotrichum acutatum, casual agents of gray mold and anthracnose diseases in strawberry. Notably, this is the first time that A. pullulans has been tested against C. acutatum in strawberry. A. pullulans strains (AP-30044, AP-30273, AP-53383, and AP-SLU6) showed significant variation in terms of growth and conidia production. An inverse relationship was found between the growth and conidiation rate, suggesting a trade-off between resource allocation for growth and conidial production. Dual plate co-culturing assays showed that mycelial growth of B. cinerea and C. acutatum was reduced by up to 35 and 18%, respectively, when challenged with A. pullulans compared with control treatments. Likewise, culture filtrates of A. pullulans showed varying levels of antifungal activity against B. cinerea and C. acutatum, reducing the mycelial biomass by up to 90 and 72%, respectively. Furthermore, milk powder plate assays showed that A. pullulans produced substantial amounts of extracellular proteases, which are known to degrade fungal cuticle. Ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS) analyses revealed that A. pullulans produced exophilins, liamocins, and free fatty acids known to have antifungal properties. A. pullulans shows high potential for successful biological control of strawberry diseases and discuss opportunities for further optimization of this beneficial fungus.

Integration of Chitosan and Biopesticides to Suppress Pre-Harvest Diseases of Apple

The natural product chitosan has been shown to reduce plant disease severity and enhance the efficacy of microbial biocontrol agents in several crops. However, little is known about the potential synergisms between chitosan and biopesticides and best use practices in apple production. The objectives of this study were to evaluate the effect of pre-harvest applications of chitosan alone and in combination with a commercial biopesticide to suppress fungal diseases of apple and to investigate the potential for chitosan to reduce the quantity of overwintering Venturia inaequalis spores in orchard leaf litter. Chitosan products, Tidal Grow and ARMOUR-Zen 15, and a commercial biopesticide, Serenade ASO, were tested in a research orchard in Pennsylvania and commercial orchards in New Hampshire. Chitosan applications reduced apple scab incidence and severity by up to 55% on fruit compared to the water control. Chitosan also reduced sooty blotch, flyspeck, and rust incidence on fruit. Furthermore, a chitosan + biopesticide treatment overlayed onto a grower standard spray program reduced diseases more effectively than the grower standard alone. However, this efficacy was dependent on the cultivar and pathogen. Chitosan did not reduce overwintering V. inaequalis ascospores. This research provides evidence that pre-harvest chitosan applications have the potential for disease management in apple production.

Glucosinolate-extracts from residues of conventional and organic cultivated broccoli leaves (Brassica oleracea var. italica) as potential industrially-scalable efficient biopesticides against fungi, oomycetes and plant parasitic nematodes

This study aimed to standarize a protocol for obtaining a bioactive extract from broccoli (Brassica oleracea var. italica) crop residues, that is suitable for application on an industrial scale and effective in reducing plant disease incidence. For this purpose, the influence of several extraction factors in the glucosinolate (GSL) content was studied with leaves collected from two conventional broccoli fields and two organic broccoli fields. The analysis showed that lyophilization had no influence on the GSL content. Storage of plant material under two different temperatures (− 20ºC and − 80ºC) had no influence on the GSLs content of the extracts. Phytotoxicity of the extracts was studied with six different plant seeds, and also cytotoxicity was determined with human liver cells in vitro. The extracts were phytotoxic at dilutions above 10%, while cell toxicity was low. Extracts concentrations of 0.1%, 1% and 2% were tested in vitro against eight plant pathogenic fungi and two oomycetes in solid and in liquid media. The extracts reduced the growth of several plant pathogenic fungi at 2% dilution by up to 38.37% against Alternaria alternata and up to 46.55% against Sclerotinia sclerotiorum. When combined with myrosinase enzyme the effect of the extracts was enhanced, reaching inhibition values of 67.06% against A. alternata in solid medium and 68.52% against Rhizoctonia solani in liquid medium. In contrast, the same extracts increased the growth of the plant pathogenic oomycetes Pythium ultimum and Phytophthora cactorum. The effect of the same extracts in the free leaving larvae, J2s, of the plant-parasitic nematode (PPN) Meloidogyne javanica was not obvious. Minor significant differences were obtained but with no clear dose-response in nematode mortality, and no inhibition of eggs hatching was observed. These results show the industrial potential of using broccoli residues to obtain extracts with biopesticide activity against plant pathogenic fungi.

An Intelligent Approach to Reducing Plant Disease and Enhancing Productivity Using Machine Learning

Plant diseases are a normal part of the natural world, and they are one of the many ecological processes that work together to keep the vast number of living organisms in the world in a state of equilibrium with one another. Each plant cell has its own set of signalling pathways that help the plant fight off viruses, animals, and insects. Concerns have been raised about whether or not it is possible to use machine learning to make crop predictions based mostly on weather data. The goal of the research is to help users choose the right crop to grow so that they can maximise their yield and, as a result, the money they make from the project. In a rural area where almost half the people work in agriculture, one of the most important problems is when farmers can't use traditional or other non-scientific methods to choose a crop that will grow well in their soil. Researchers can't make use of case studies as well as they could because there isn't enough correct and up-to-date information available. With the resources at our disposal, we have proposed a model that makes use of random forests and the genetic algorithm. This model has the potential to solve this problem by providing predictive insights on the long-term viability of crops and recommendations based on machine-reading models that have been trained to take important environmental parameters into consideration..

Inhibition of Botrytis cinerea Growth and Suppression of Gray Mold on Petunia Leaves Using Chitosan.

Exogenous application of chitosan has been shown to reduce plant disease severity in food crops; however, less is known about the potential use of chitosan in floriculture. The objective of this study was to investigate the effectiveness of chitosan to suppress gray mold on petunia leaves caused by Botrytis cinerea using in vitro and in planta approaches. We also aimed to determine if chitosan molecular weight influences efficacy. Medium and high molecular weight reagent grade chitosan reduced growth of B. cinerea in vitro at chitosan concentrations ranging from 1.25 to 2% (v/v), while low molecular weight reagent grade chitosan only reduced growth at 2.0% (v/v). In detached leaf assays, all reagent grade chitosan treatments reduced Botrytis lesion size on petunia leaves up to 65% compared to the water control. The commercial product Tidal Grow reduced in vitro growth of Botrytis, starting at 0.5%, and reduced disease severity at 0.75% on petunia leaves. The commercial product ARMOUR-Zen 15 reduced Botrytis growth in vitro at 3.75% and higher and reduced disease severity at 0.3 and 1.0% on petunia leaves. Under greenhouse conditions, low, medium, and high molecular weight reagent grade chitosan and ARMOUR-Zen 15 at 0.4% chitosan reduced Botrytis lesion size on petunia leaves up to 60% compared to the water control. Suppression in vitro suggests that chitosan may have direct phytotoxic effects on fungal growth, however our in planta and greenhouse trials suggest that additional modes of action may also play a role in the observed suppressive effects.

RETRACTED: Decrypting the multi-functional biological activators and inducers of defense responses against biotic stresses in plants

Plant diseases are still the main problem for the reduction in crop yield and a threat to global food security. Additionally, excessive usage of chemical inputs such as pesticides and fungicides to control plant diseases have created another serious problem for human and environmental health. In view of this, the application of plant growth-promoting rhizobacteria (PGPR) for controlling plant disease incidences has been identified as an eco-friendly approach for coping with the food security issue. In this review, we have identified different ways by which PGPRs are capable of reducing phytopathogenic infestations and enhancing crop yield. PGPR suppresses plant diseases, both directly and indirectly, mediated by microbial metabolites and signaling components. Microbial synthesized anti-pathogenic metabolites such as siderophores, antibiotics, lytic enzymes, hydrogen cyanide, and several others act directly on phytopathogens. The indirect mechanisms of reducing plant disease infestation are caused by the stimulation of plant immune responses known as initiation of systemic resistance (ISR) which is mediated by triggering plant immune responses elicited through pathogen-associated molecular patterns (PAMPs). The ISR triggered in the infected region of the plant leads to the development of systemic acquired resistance (SAR) throughout the plant making the plant resistant to a wide range of pathogens. A number of PGPRs including Pseudomonas and Bacillus genera have proven their ability to stimulate ISR. However, there are still some challenges in the large-scale application and acceptance of PGPR for pest and disease management. Further, we discuss the newly formulated PGPR inoculants possessing both plant growth-promoting activities and plant disease suppression ability for a holistic approach to sustaining plant health and enhancing crop productivity.

Prediction of grape leaf through digital image using FRCNN

A great deal of research indicates that the duality of agricultural production can be reduced because of various factors. Plant diseases are among the most critical aspects of this category. As a result, reducing plant diseases allows for significant improvements in quality. The article uses Grape Leaf Disease Detection Technique (GLDDT) with Faster Region based Convolutional Neural Network (FRCNN) - GLDDT-FRCNN techniques to automatically diagnose plant diseases. Once trained, the software can diagnose plant leaf disease without requiring any experimentation. The primary focus of this research is grape leaf disease. The basic technique manipulates H & colour histograms 25 channels 24. Excluding the final 26 phases, where the person decides which channel (H or a) offers the best separation, the algorithm method is fully automated. A GLDDT is also proposed in this article, which uses two-pronged processes for the evaluation, recognition and categorization of traits. The analysis process, testing on a benchmark set of data reveals that the disease diagnosis system might be a better fit than existing methods because it recognizes and identifies infected/diseased areas. The researchers achieved a precision rate of 99.93% for the detection of Isariopsis, black rot and Esca using the proposed disease detection method.

Nanotechnology in Crop Protection: A Review

The establishment and development of new pathogenic races is a constant problem, because chemical pest control is both expensive and ineffectual. Nanomaterials have lately been proposed as a potential alternative for reducing plant diseases and crop protection. Agricultural methods usually include the systematic administration of a variety of active chemicals at varied dosages and frequency, resulting in a variety of selective regimes. Crop protection is critical in the production of food all over the world. Nanotechnologies are being employed more and more to maintain traditional crops and to develop novel crops with superior qualities. Pollen magnetofection and gene nanocarriers are two nanobiotechnologies that are now being used to improve pest, weed, and disease management, as well as agricultural genetic modification. Traditional crop pest, weed, and disease management has been greatly enhanced thanks to a better understanding of the synthesis of nanomaterials with extraordinary capabilities. Plant germination, growth, and crop protection have all been proved to benefit from nanoparticles in agriculture. The enhanced specific surface area of nanomaterials benefits fertilisers and insecticides. Nanomaterials have also been developed for a variety of applications such as medical, medication delivery, electronics, fuel cells, solar cells, food, space, and so on. This chapter outlines recent attempts the use of nanotechnologies in agriculture in novel ways that could assist meet rising food demand while also ensuring environmental sustainability.