Fish-flavoured spicy tamarind cube is categorized as a type of bouillon cube. It is a processed food that has been developed and benefits the consumers due to low cost, being ready to use, reduced cooking time and giving intense flavour to the cooking dish. Food processing improves the stability, safety, taste and makes the food healthier. While there are various advantages to processing, the chemical food characteristics might be adversely affected. The study aimed to evaluate the amount of chemical composition, macromolecule, phytochemical content, peroxide value and antioxidant activity present in the fish-flavoured spicy tamarind cube. The analysis was performed using a different methodology. The content of mineral and ascorbic acid of the cube was evaluated by using scanning electron microscopy-energy dispersive X-ray (SEM-EDX) and titration methods respectively. Carbohydrate was estimated by difference using equation and protein by micro Kjeldahl method. For both phytochemical compounds, total phenolic and flavonoid are measured spectrophotometrically in this study. In addition, peroxide value was determined by titration method and antioxidant activity was assayed using 2,2- diphenyl-1-picrylhydrazyl (DPPH) free radical. The result showed the cube contained 2.12% sodium, 1.22% copper and 0.43% potassium while the ascorbic acid value was 66.00 mg/100 g. The macromolecule content recorded was 40.15 g/100 g for carbohydrates and 7.54 g/100 g for protein. The cube had 10.77 mg GAE/g and 21.72 mg QE/g for total phenolic and flavonoid respectively. The peroxide value of the cube was 9.52 meq/kg and classified in a moderate oxidation state. Furthermore, the half-maximal inhibitory concentration (IC50) for antioxidant activity analysed was 89.04 µg/mL which means the cube shows intermediate antioxidant activity. Based on the result, the cube is safe to consume and can provide health benefits to consumers.
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