Red Swamp Crayfish Procambarus Clarkii Research Articles

Parabens and triclosan in red swamp crayfish (Procambarus clarkii) from China: Concentrations, tissue distribution and related human dietary intake risk

Parabens (PBs) and triclosan (TCS) are commonly found in pharmaceuticals and personal care products (PPCPs). As a result, they have been extensively found in the environment, particularly in aquaculture operations. Red swamp crayfish (Procambarus clarkii) consumption has significantly risen in China. Nevertheless, the levels of PBs and TCS in this species and the associated risk to human dietary intake remain undisclosed. This study assessed the amounts of five PBs, i.e., methyl-paraben (MeP), ethyl-paraben (EtP), propyl-paraben (PrP), butyl-paraben (BuP) and benzyl-paraben (BzP), as well as TCS in crayfish taken from five provinces of the middle-lower Yangtze River. MeP, PrP and TCS showed the highest detection rates (hepatopancreas: 46–86 %; muscle: 63–77 %) since they are commonly used in PPCPs. Significantly higher levels of ∑5PBs (median: 3.69 ng/g) and TCS (median: 7.27 ng/g) were significantly found in the hepatopancreas compared to the muscle (median: 0.39 ng/g for ∑5PBs and 0.16 ng/g for TCS) (p < 0.05), indicating bioaccumulation of these chemicals in the hepatopancreas. The estimated daily intake values of ∑5PBs and TCS calculated from the median concentrations of crayfish were 6.44–7.94 ng/kg bw/day and 11.4–14.0 ng/kg bw/day, respectively. Although no health risk was predicted from consuming crayfish (HQ <1), consumption of the hepatopancreas is not recommended.

Effects of luteolin supplementation on growth, histology, antioxidant capacity, non−specific immunity and intestinal microbiota of the red swamp crayfish (Procambarus clarkii)

To investigate the effects of luteolin (LUT) on the growth, antioxidant capacity, non−specific immunity and intestinal microbiota of red swamp crayfish (Procambarus clarkii), 300 individual crayfish (4.17 ± 0.50 g average body weight and 50.19 ± 1.40 mm average body length) were randomly divided into five groups and fed with LUT doses of 0 mg/kg (CG), 50 mg/kg (LG50), 100 mg/kg (LG100), 300 mg/kg (LG300), and 500 mg/kg (LG500) for 8 weeks, respectively. Firstly, the results demonstrated that the survival and growth performance were significantly improved in all LUT added groups compared to the control (CG group), with no significant differences in hepatosomatic index, feed coefficient and muscle components, and the crayfish in LG100 group showed the maximal survival rate (SR) and specific growth rate (SGR) (P < 0.05). Secondly, histological observations displayed that the hepatopancreas and intestine tissues were not impacted in the LUT−supplemented groups, and the length of intestinal folds was significantly increased in LG100 and LG300 groups. Thirdly, LG50, LG100, and LG300 groups produced significantly increased hepatopancreatic antioxidant enzyme activities and hemolymph immune indicators compared with the control. In all experimental groups, malondialdehyde (MDA) contents were significantly reduced, as were aspartate aminotransferase and alanine aminotransferase activities. Lastly, dietary LUT specifically improved the structure and optimized the function of the gut microbiota. Compared to the control, the LG100 group displayed a significant increase in the index of Chao1 and Observed species, an increase in the relative abundance of Proteobacteria and Actinobacteria, a decrease in the ratio of Bacteroidetes to Firmicutes, and an increase in the abundance of Thermomonas, Turicibacter, Bdellovibrio and Enterococcus. PICRUSt analysis demonstrated a significant improvement in the KEGG pathway associated with isoflavone biosynthesis in the LG group. In conclusion, the present study confirms that dietary supplementation with 50−300 mg/kg LUT favors growth, non−specific immunity and antioxidant function in P. clarkii, with optimal additions ranging from 102−165 mg/kg. Furthermore, it optimized gut microbiota function, suggesting that LUT can be used as a potential feed additive for P. clarkii. These findings provide new insights for crayfish’s healthy culture.

Prevalence of the crayfish plague pathogen in red swamp crayfish populations in western France: How serious is the risk for the native white-clawed crayfish?

The crayfish plague pathogen Aphanomyces astaci has been implicated in a number of mass mortalities and irreversible population declines of native crayfish across Europe. At present, the reservoirs of the pathogen in Europe are mainly populations of invasive North American crayfish species. In southwestern Europe, including France, a particularly widespread invader is the red swamp crayfish Procambarus clarkii. Recent distribution data confirm that P. clarkii is present in at least 75 French departments, i.e. more than 78% of those in metropolitan France. We analysed the prevalence and pathogen load of A. astaci in 42 populations of this species in western France (Nouvelle Aquitaine region), where the species is most densely distributed, particularly in a wide range of environments around the Gironde estuary. The pathogen was detected by two different quantitative PCR assays in more than three quarters of the populations studied (34 out of 42); 163 out of 480 analysed crayfish individuals tested positive for the presence of A. astaci. In most cases, individual infection levels were very low, detectable with quantitative PCR but not sufficient for pathogen genotyping. In seven P. clarkii individuals from four populations, however, we were able to assess A. astaci variation by microsatellite markers and sequencing of mitochondrial markers. All these host specimens carried A. astaci genotype group D, haplotype d1, which has caused the majority of crayfish plague outbreaks in neighbouring Spain. In contrast, the French outbreaks genotyped to date (including eight newly analysed in this study) were mostly caused by strains of genotype group B, specific to the signal crayfish Pacifastacus leniusculus. Haplotype d1 found in P. clarkii was involved in one of the newly characterised outbreaks. Our study confirms that P. clarkii is a potentially important reservoir of the crayfish plague pathogen in France, but not the main source of the pathogen in mass mortalities of A. pallipes, probably due to different ecological requirements of the different invasive host crayfish. However, as P. clarkii continues to spread, the threat posed by this species to native crayfish is likely to increase.

Comparative transcriptome analysis reveals the immune defense mechanism of the red swamp crayfish (Procambarus clarkii) in response to Vibrio parahaemolyticus challenge

Comparative transcriptome analysis reveals the immune defense mechanism of the red swamp crayfish (Procambarus clarkii) in response to Vibrio parahaemolyticus challenge

Toxic mechanisms of nanoplastics exposure at environmental concentrations on juvenile red swamp crayfish (Procambarus clarkii): From multiple perspectives

Nanoplastics pollution has emerged as a global issue due to its widespread potential toxicity. This study delved in to toxic effects of nanoplastics on juvenile P. clarkii and molecular mechanisms from perspectives of growth, biochemical, histopathological analysis and transcriptome level for the first time. The findings of this study indicated that nanoplastics of different concentrations have varying influence mechanisms on juvenile P. clarkii. Nanoplastics have inhibitory effects on growth of juvenile P. clarkii, can induce oxidative stress. The biochemical analysis and transcriptome results indicated that 10 mg/L nanoplastics can activate the antioxidant defense system and non-specific immune system in juvenile P. clarkii, and affect energy metabolism and oxidative phosphorylation. While 20 mg/L and 40 mg/L have a destructive influence on the immune function in juvenile P. clarkii, leading to lipid peroxidation and oxidative damage, and induce apoptosis, can affect ion transport and osmotic pressure regulation. The findings of this study can offer foundational data for delving further into impacts of nanoplastics on crustaceans and toxicity mechanism.

Isolation and identification of intestinal cellulolytic bacteria from red swamp crayfish (Procambarus clarkii)

Red swamp crayfish (Procambarus clarkii) like to eat aquatic plants. The intestinal microorganism plays an important role in cellulose degradation and utilization. Isolating bacteria which can degrade cellulose from the intestines of P. clarkii can provide a theoretical basis for the development of probiotics in forage for P. clarkii. Three selective media including carboxymethyl-cellulose, microcrystalline cellulose, and cellobiose were used in plate cultures for 48h and then dyed with Congo red. It was found that 5 strains produced hydrolytic rings on 3 culture media at 48h. Based on the results of 16S rRNA molecular analysis, strains C, E, G, H, and M were identified as Citrobacter sp., Staphylococcus sp., Acinetobacter johnsonii, Shewanella sp., and Aeromonas caviae, respectively. Specifically, Staphylococcus sp. exhibited the strongest capacity for the degradation of cellulose. Shewanella sp. showed the strongest degradation capacity for cellobiose. Acinetobacter johnsonii and Shewanella sp. can degrade cellulose and are expected to be used as probiotic feed for P. clarkii. This study can provide a theoretical basis for the healthy culture of P. clarkii.

Concentrations, sources and health risk of bisphenols in red swamp crayfish (Procambarus clarkii) from South-Eastern China

Bisphenol analogs (BPs) are extensively employed in commercial and industrial products and they have been found in a variety of environmental matrices and human samples. The red swamp crayfish (Procambarus clarkii) has been a trendy food in China in recent decades. However, the levels of BPs in Chinese crayfish and the associated hazards of human exposure remain unknown. Thus, in this study, the levels of eight BPs in crayfish gathered from five major provinces engaged in crayfish within the Yangtze River Basin were analyzed. Additionally, the health risks for humans by ingesting crayfish were calculated. BPs were frequently detected in crayfish tissues, indicating the wide occurrence of these chemicals. In comparison to other substitutions, BPA remains the dominant bisphenol analog. Most of the BPs were observed to accumulate in the hepatopancreas compared to the muscle, so consuming the hepatopancreas of crayfish is not recommended. With the exception of BPS, the Estimated Daily Intakes (EDIs) of the remaining BPs exceeded the Tolerable Daily Intake (TDI) specified by the European Food Safety Authority (EFSA) by a factor of 1.75–69.0. The mean hazard index (HI) values exceeded 1 for both hepatopancreas and muscle in all provinces, and the mean HI values for hepatopancreas were significantly higher than those for muscle, indicating potential health risks for local consumers.

Mechanical excavation of wetland habitat failed to eradicate invasive American red swamp crayfish (Procambarus clarkii) in Malta

Abstract Invasive crayfish are an important ecological concern in many freshwater ecosystems. Many efforts have been made to eradicate them, but there is very little documentation of the effectiveness of these efforts. Between 2019 and 2020, a restoration project funded by the European Regional Development Fund tried to eradicate invasive American red swamp crayfish Procambarus clarkii from the Fiddien valley system in Malta by mechanically excavating the valley's stream bed and exporting contaminated debris securely off‐site to a dry quarry. Three years post‐intervention, we systematically surveyed the valley system to explore the distribution and relative abundance of the invasive crayfish population. We placed traps in a stratified random sample of stream segments (both those that were included in the original restoration project and those that were not) and recorded catch per unit effort (crayfish caught per trap night) and the size/frequency distribution of crayfish caught. The invasive crayfish were still abundant in the upper reaches of the valley system, and, despite the excavation effort, crayfish were present at the highest relative abundance (4.3–14.8 CPUE, median = 12.3) within the restored area. Despite substantial effort and spending of more than 700,000 €, mechanical excavation did not eradicate invasive crayfish populations. We urge caution for future projects planning to attempt crayfish eradication using this approach and call for much greater impact evaluation, and at the very least post‐project monitoring, to ensure lessons can be learnt from such failures in future.

The first finding of the red swamp crayfish Procambarus clarkii in Greece calls for rapid measures

AbstractThis contribution presents the first record of the invasive non-native red swamp crayfish Procambarus clarkii (Girard, 1852) in Greece and the Balkans. The crayfish was found during a fish monitoring expedition in the lower reaches of the Vosvozis River, near the city of Komotini, Thrace (northeastern Greece). The finding of P. clarkii in Greece expands the southernmost geographical range of the species in the Balkans and calls for immediate actions in preventing its further expansion to other water bodies and towards strict control of aquarium trade.

Differentially expressed genes involved in immune pathways following Vibro parahaemolyticus challenge: A study on red swamp crayfish Procambarus clarkii

This study conducted a comprehensive analysis of the damage mechanism of Vibrio parahaemolyticus (V. parahaemolyticus) on crayfish, utilizing pathological section observations, biochemical indicator detection, and transcriptomics analysis. Histopathological examination showed fragmentation of the gill lamellae, spotted pigment deposition on the branchial membrane, degeneration of epithelial cells, and disruption of the normal structure of gill tissues. Biochemical tests indicated a significant decrease in the activity of antioxidant-related enzymes in crayfish injected with V. parahaemolyticus compared with the phosphate-buffered saline group. Transcriptomics analysis identified 2307 upregulated and 1721 downregulated differentially expressed genes (DEGs). Gene ontology enrichment analysis highlighted that DEGs were primarily involved in the regulation of lipid metabolic processes, cellular nitrogen compound biosynthesis and metabolic processes, and the negative regulation of neuron death. Kyoto Encyclopedia of Genes and Genomes pathway analysis revealed significant enrichment in N-glycan biosynthesis, fatty acid metabolism, and the mTOR signaling pathway. Gene set enrichment analysis indicated pathways related to immunity and oxidative stress, including oxidative phosphorylation and tyrosine metabolism. Quantitative reverse transcription–polymerase chain reaction results for selected genes involved in the oxidative phosphorylation pathway and immune-related genes corroborated the RNA-Seq trends, confirming the transcriptomics data's reliability. The findings suggest that infection with V. parahaemolyticus leads to excessive oxidative stress due to bacterial proliferation, overwhelming the crayfish's antioxidative defense. This causes a continuous increase in reactive oxygen species levels, leading to irreversible damage to the oxidative phosphorylation and innate immune systems. This study elucidated the mechanism of oxidative stress damage in crayfish following V. parahaemolyticus infection, providing valuable insights for reducing bacterial and viral diseases in aquaculture. A deeper understanding of pathogen affects the physiology and immune systems of aquatic organisms can help develop more effective prevention and control strategies, thereby improving the efficiency and sustainability of aquaculture.

PHB2 inhibits WSSV replication by promoting the nuclear translocation of STAT

Prohibitins (PHBs) are ubiquitously expressed conserved proteins in eukaryotes that are associated with apoptosis, cancer formation, aging, stress responses and cell proliferation. However, the function of the PHBs in immune regulation has largely not been determined. In the present study, we identified PHB2 in the red swamp crayfish Procambarus clarkii. PHB2 was found to be widely distributed in several tissues, and its expression was significantly upregulated by white spot syndrome virus (WSSV) challenge. PHB2 significantly reduced the amount of WSSV in crayfish and the mortality of WSSV-infected crayfish. Here, we observed that PHB2 promotes the nuclear translocation of STAT by binding to STAT. After blocking PHB2 or STAT with antibodies or interfering with PHB2 or STAT, the expression levels of the antiviral genes β-thymosin (PcThy-4) and crustin2 (Cru2) decreased. The gene sequence of PHB2 was analyzed and found to contain a nuclear introgression sequence (NIS). After in vivo injection of PHB2 with deletion of NIS (rΔNIS-PHB2), the nuclear translocation of STAT did not change significantly compared to that in the control group. These results suggest that PHB2 promoted the nuclear translocation of STAT through NIS and mediated the expression of antiviral proteins to inhibit WSSV infection.

Comparative study on the utilization of four forms exogenous methionine by red swamp crayfish (Procambarus clarkii)

To investigate the efficacy of four forms of methionine (Met) supplementation in red swamp crayfish (Procambarus clarkii), a Met-deficient all-plant protein diet was used control, along with four supplemental diets: 0.90 g/kg Met-Met, 1.80 g/kg crystal DL-Met, 3.60 g/kg E-Met, and 3.29 g/kg MHA-Ca. The analysis Met + Cys levels of five diets were 10.46 (control), 11.51, 12.50, 12.56, and 10.70 g/kg, respectively. Each diet was hand-fed to five replicates of 18 crayfish each (initial body weight: 4.40 ± 0.02 g) for eight weeks. Supplementation with four forms of exogenous Met resulted in an increase in weight gain rate, specific growth rate, and antioxidant capacity, while decreasing the feed conversion ratio (P < 0.05). The abdominal muscle crude protein content and the protein deposition rate in the Met-Met and E-Met groups were higher than those of the control group (P < 0.05). The deposition rate of Met, arginine, leucine, lysine, threonine, and valine, and the activities of digestive enzymes were higher in the Met-Met group than that of the control group (P < 0.05). The peak times and values of serum biochemical indices were affected by four forms of exogenous Met. Additionally, the hepatopancreatic homocysteine content in the MHA-Ca group was higher than in the control group (P < 0.05). In conclusion, the utilization of Met-Met by Procambarus clarkii was superior to other forms of Met in an all-plant protein diet.

The effects of salinity on the distribution and survival of two exotic ostracods in the Iberian Peninsula

Recent research highlighted the need to include experimental estimates of tolerance limits to varying environmental conditions when investigating what factors limit species distributions. However, most niche approaches are only based on the statistical dependence between environmental and occurrence data. Here, we combined field data with survival experiments to assess the role of salinity as a limiting factor in the distribution of two species of exotic ostracods from the Iberian Peninsula. Vizcainocypria viator is a free-living species associated with rice fields and Ankylocythere sinuosa is a commensal of the red swamp crayfish (Procambarus clarkii). Experiments and field data indicate that the distribution of V. viator is limited by adult survival at low and high salinities (below electrical conductivity of 0.6 mS/cm and above 10 mS/cm). In the case of A. sinuosa, the analysis of field data shows that its prevalence is negatively affected by high salinity, whereas experiments indicate an optimal survival at high salinities (conductivity above 10.2 mS/cm), thus suggesting that high salinity may impact A. sinuosa distribution indirectly through affecting host traits (e.g. reduced activity). The habitat of close ancestors (marine versus non-marine respectively for A. sinuosa and V. viator) most likely explains the contrasting differences in salinity tolerance between both ostracod species.

Ecological Toxicity of Cyantraniliprole against Procambarus clarkii: Histopathology, Oxidative Stress, and Intestinal Microbiota.

Cyantraniliprole is a novel insecticide recently introduced for rice pest control that may cause potential threats to the red swamp crayfish (Procambarus clarkii) in rice-crayfish coculture systems. In this study, we investigated the acute toxicity of cyantraniliprole against P. clarkii with a LC50 value of 149.77 mg/L (96 h), first. Some abnormal behaviors of P. clarkii treated with 125 mg/L cyantraniliprole, including incunabular hyperexcitability, imbalance, inactivity, and increased excretion were observed. Moreover, it was observed that exposure to 5 mg/L cyantraniliprole for 14 days resulted in histopathological alterations in abdominal muscle, gills, hepatopancreas, and intestines. Furthermore, exposure to 0.05 and 5 mg/L cyantraniliprole induced increased activities of several oxidative stress-related enzymes, which was verified by the upregulation of related genes. Additionally, dysregulation of the intestinal microbiota was determined via 16S rRNA sequencing. These results will provide the basis for the utilization of cyantraniliprole in the fields of rice-crayfish integrated system.

Effects of Hypoxia Stress on Survival, Antioxidant and Anaerobic Metabolic Enzymes, and Related Gene Expression of Red Swamp Crayfish Procambarus clarkii.

The red swamp crayfish Procambarus clarkii is the most reared shrimp in China, but it is often affected by hypoxia stress in the process of seedling culture and adult crayfish culture. The oxygen consumption rate and asphyxiation point of juvenile crayfish (1.17 ± 0.03 g) and subadult crayfish (11.68 ± 0.11 g) at different temperatures (20, 22, 24, 26, and 28 °C) were studied. The survival, glycolysis, and expression of antioxidant genes were compared under 24 h acute hypoxia stress (1, 2, and 3 mg/L) and normal dissolved oxygen (7.5 mg/L). The results showed that the oxygen consumption rate and asphyxiation point of juvenile and subadult crayfish increased with increasing temperatures (20-28 °C). At the same temperature, the oxygen consumption rate and asphyxiation point of juvenile crayfish were significantly higher than those of subadult crayfish (p < 0.05). Within 24 h, the three hypoxia stress environments did not lead to the death of crayfish, indicating that P. clarkii has a strong ability to adapt to hypoxia. Hypoxia stress significantly affected the activities of antioxidant and anaerobic metabolic enzymes and gene expression in juvenile and subadult crayfish. The activities of the superoxide dismutase (SOD), catalase (CAT), and lactate dehydrogenase (LDH) and the content of lactic acid (LD) in the hepatopancreas of juvenile and subadult crayfish in the hypoxia stress groups increased significantly. The expression levels of SOD mRNA, CAT mRNA, Hsp70 mRNA, and crustin 4 mRNA in the hepatopancreas of juvenile and subadult crayfish in the hypoxia stress groups were significantly higher than those in the control group (p < 0.05), and the higher the degree of hypoxia stress, the higher the expression of each gene. The results showed that the antioxidant system of juvenile crayfish was more sensitive to hypoxia environments, and hypoxia stress resulted in increased stress levels in juvenile crayfish and subadult crayfish.

Structural and functional analysis of transforming growth factor beta regulator 1 (TBRG1) in the red swamp crayfish Procambarus clarkii: The initial insight into TBRG1's role in invertebrate immunity

The transforming growth factor beta regulator 1 (TBRG1) is a growth inhibitory protein that acts as a tumor suppressor in human cancers, gaining its name for the transcriptional regulation by TGF-β. While extensive research has been conducted on the tumor-related function of TBRG1 in mammals, its significance in invertebrates remains largely unexplored. In this study, a homolog of TBRG1 was first structurally and functionally analyzed in the red swamp crayfish Procambarus clarkii. The full-length cDNA sequence was 2143 base pairs (bp) with a 1305 bp open reading frame (ORF) encoding a deduced protein of 434 amino acids (aa). The changes of PcTBRG1 transcripts upon immune challenges indicated its involvement in innate immunity. After knocking down PcTBRG1, the decline of bacteria clearance capacity revealed the participation of PcTBRG1 in the immune response. Furthermore, the downregulation of AMPs' expression after the cotreatment of RNAi and bacteria challenge suggested that PcTBRG1 might participate in innate immunity through regulating AMPs’ expression. These results provided initial insight into the immune-related function of TBRG1 in invertebrates.

Effects of the invasive red swamp crayfish (Procambarus clarkii) on eelgrass-dominated clearwater lakes: the role of bioturbation

Effects of the invasive red swamp crayfish (Procambarus clarkii) on eelgrass-dominated clearwater lakes: the role of bioturbation

From Fresh to Salt Waters: First Reports of the Red Swamp Crayfish Procambarus clarkii (Girard, 1852) in Mediterranean Marine Waters

The red swamp crayfish Procambarus clarkii (Girard, 1852), native to the southern/southeastern United States and northern Mexico, has been introduced in many countries worldwide. The species has a remarkable invasiveness due to its high reproductive rate and ability to tolerate broad ranges of water parameters like salinity. Nevertheless, no consistent observations of P. clarkii have been reported in the marine environment until now. Data demonstrating the presence of the crayfish within marine ecosystems were collected by a personal acquaintance and two volunteers who published photos of the species on Facebook marine biology and fishing groups. The species was identified based on photos taken by these people in the described areas. P. clarkii has been spotted in three different localities, in two of which observations are recurrent. The species can survive miles from the nearest freshwater stream and could potentially colonise marine waters at depths up to 20 m. The multiple observations of P. clarkii collected in our study uncover a persistent presence of the species within some marine areas, rather than its mere sporadic occurrence. Our results highlight the need for further investigation to obtain a comprehensive understanding of the crayfish’s ability to withstand salinity and colonise the marine environment.

Responses of adult crayfish to macro-nutrients intake alteration during juvenile stage on metabolism and intestinal microbiota

Red swamp crayfish Procambarus clarkii is becoming an ecologically and economically important crustacean species in China. In present study, whether the macro-nutrients intake intervention during early life in crayfish resulting long-term influences on nutritional use and metabolism were evaluated in view of the concept of nutritional programming effects. Juvenile crayfish underwent a 14 days of high-carbohydrate (43%) low-protein (17%) nutritional stimulus, following a 70 days of routine dietary feeding (carbohydrate 15%, protein 36%), until adulthood. Short- (14 days) and long-term (84 days) effects were evaluated respectively in terms of growth performances, digestive enzymes activities, body compositions, and intestinal microbiota (long-term only). Data showed that in the short term, it enhanced the activities of amylase and lipase but reduced the activity of trypsase in hepatopancreas. In the long term, it decreased the growth performances (SR and WGR) and modified the diversity of intestinal microbiota obviously (p < 0.05). Throughout the period it increased body crude protein level. All results indicated that early nutritional events caused long-term impacts on nutrient use thus affect physiology and growth until adulthood. In short, present work provided evidences to support the existence of nutritional programming effects in juvenile crayfish.

The effects of bisphenol S exposure on the growth, physiological and biochemical indices, and ecdysteroid receptor gene expression in red swamp crayfish, Procambarus clarkii

The experiment was conducted to investigate the effects of Bisphenol S (BPS) on growth, physiological and biochemical indices, and the expression of ecdysteroid receptor (ECR) of the red swamp crayfish (Procambarus clarkii). The gene encoding ECR was isolated from red swamp crayfish by homologous cloning and rapid amplification of cDNA ends (RACE). The ECR transcripts were 1757 bp long and encoded proteins of 576 amino acids. The quantitative real-time PCR (qRT-PCR) analysis showed that the ECR gene was expressed in various tissues under normal conditions, and the highest level was observed in the ovary and the lowest level was observed in the muscle (P < 0.05). Then, the experiment was designed with four different BPS concentrations (0, 1, 10, and 100 μg/L), BPS exposure for 14 days, three parallel groups, and a total of 240 red swamp crayfish. At 100 μg/L BPS, the survival rate, weight gain rate, and relative length rate were decreased significantly (P < 0.05). Malonaldehyde (MDA) content reached the highest level at 100 μg/L BPS. When BPS concentration was higher than 10 μg/L, the activities of superoxide dismutase (SOD) and catalase (CAT) were significantly lower than those of the control group (P < 0.05). The expression levels of the ECR gene in ovary, intestinal, gill, and hepatopancreas tissues were significantly increased after BPS exposure (P < 0.05). The ECR gene expression in ovaries and Y-organs was significantly higher than other groups in 10 μg/L BPS (P < 0.05). The expressions of the tumor necrosis factor -α (TNF-α) and interleukin-6 (IL-6) genes in the hepatopancreas gradually increased, and the highest expression was observed exposed in 100 μg/L BPS (P < 0.05). This research will provide novel insights into the health risk assessment of BPS in aquatic organisms.