Recovery Of Folic Acid Research Articles

A Review of Microfiltration Membrane in Separating Folic Acid from Fermented Dark Green Leafy Vegetables (DGLVs), Legumes, Beans, and Cereal for Nutraceutical Application

DGLVs (spinach, broccoli), legumes (soybean, mung bean, kidney bean), and cereal (corn) are well recognized for human health benefits due to the presence of natural folate (vitamin B9) with essential micronutrients. Folate is among the most unstable vitamins for the normal functions of the human body. The recent development of membrane-based methods for the separation of folic acid (FA) is reviewed from the selected literature and covers the result of significant research that has been performed in the last five years on the use of the microfiltration (MF) method. This is coupled with the fermentation process to support and achieve separation or recovery of FA from their selected plant-based food source. The nixtamalization, fermentation, separation, and purification of DGLVs, legumes, and beans, as well as corn, were conducted using the MF (pore size 0.15 µm) membrane separation method. The results showed that the MF membrane was able to separate FA components in permeate from fermented spinach and broccoli, soybean, mung bean, kidney bean, and corn, namely 7.14 and 3.79, 197.00, 325.37 and 242.93, and 121.10 µg/mL, respectively. Meanwhile, FA content in retentate from fermented spinach and broccoli, soybean, mung bean, kidney bean, and corn was 58.90 and 28.10, 362.07, 254.07 and 506.07, and 212.84 µg/mL, respectively. The applications of membrane techniques were used as an alternative to some unit operations in the solving of separation issues as well as the development of selected new fermented plant-based food sources. The contemporary pressure-driven membranes, such as MF, and the main applications were related to pre-treatment, selected fermented plant-based food sources, and alternative technology for extending the shelf life of natural folate. The utilization of MF membranes in the nutraceutical field holds significant promise, particularly in the creation of value-added products from minor compounds.

Genetic and pharmacological inhibition of fatty acid-binding protein 4 alleviated inflammation and early fibrosis after toxin induced kidney injury

Considerable data have suggested that acute kidney injury (AKI) is often incompletely repaired and could lead to chronic kidney disease (CKD). As we known, toxin-induced nephropathy triggers the rapid production of proinflammatory mediators and the prolonged inflammation allows the injured kidneys to develop interstitial fibrosis. In our previous study, fatty acid-binding protein 4 (Fabp4) has been reported to be involved in the process of AKI. However, whether Fabp4 plays crucial roles in toxin-induced kidney injury remained unclear. To explore the effect and mechanism of Fabp4 on toxin induced kidney injury, folic acid (FA) and aristolochic acid (AA) animal models were used. Both FA and AA injected mice developed severe renal dysfunction and dramatically inflammatory response (IL-6, MCP1 and TNF-a), which further lead to early fibrosis confirmed by the accumulation of extracellular matrix proteins (α-Sma, Fn, Col1 and Col4). Importantly, we found that FA and AA induced-kidney injury triggered the high expression of Fabp4 mRNA/protein in tubular epithelial cells. Furthermore, pharmacological and genetic inhibition of Fabp4 significantly attenuated FA and AA induced renal dysfunction, pathological damage, and early fibrosis via the regulation of inflammation, which is mediated by suppressing p-p65/p-stat3 expression via enhancing Pparγ activity. In summary, Fabp4 in tubular epithelial cells exerted the deleterious effects during the recovery of FA and AA induced kidney injury and the inhibition of Fabp4 might be an effective therapeutic strategy against the progressive AKI.

Analysis of folic acid in white rice by ultra-fast liquid chromatography

The method of analysis was standardized, and the folic acid content was determined in samples of fortified white rice (Z, G and D) and unfortified (E and O). The analysis was performed in triplicate in samples of 2.0000 g, hydrolysis was performed with phosphate buffer pH 8.82 for one hour and then lowered to pH 7.00 for enzymatic treatment with takadiastase at 65°C. The extracts were filtered by gravity, purified with nylon syringe filters and ion exchange cartridges (solid phase extraction using strong anion exchange cartridges). The concentration in mg folic acid/kg of rice, in each extract was determined by ultrafast liquid chromatography, with average values for Lot1 of the fortified rice samples, of 44.03, 51.95, 38.08, and unfortified samples of 31.51 and 21.94, and with respect to Lot2 of 41.26, 41.27, 34.54 for fortified ones and 24.92 and 20.94 for non-fortified ones, respectively. The concentration of folic acid obtained by filtration through syringe filters was higher than in the extracts purified by solid phase extraction using strong anion exchange cartridges, evidencing loss of the analyte. The recovery of folic acid in the samples doped and purified by syringe filters was around 65.00%, while by solid phase extraction using strong anion exchange cartridges 43.00%. There is evidence of variation in the folic acid content between different batches of rice of the same brand.

Establishment of a Simple and Convenient Method for Folic Acid Enzyme Chemiluminescence Immunoassay

The enzyme chemiluminescence new immunoassay for folic acid (FA) was established by competition model. Add FA samples to a microtiter plate precoated with the goat anti-mouse IgG firstly, then add enzyme abled FA and FA monoclonal antibody (McAb). The values of CLIA were measured to reflect the quantity of FA. The limit of detection(LOD) of assay is 0.37ng/mL. The assay shows good correlation during 1∼30 ng/mL with correlation coefficient 0.9976. The intra- and inter-assay coefficients of variation are 4.8 % ∼ 7.3 % and 6.1 % ∼ 12.2 %, respectively. The recovery of folic acid in serum is 90.4 %∼113.2 %. Compared with determine value clinically in chemiluminescence immunoassay kit from Roche company, the correlative equation is y = 0.9689x + 0.0228, and correlation coefficient is 0.9780. Various components and kit overall show good stabilities. This method is simple and convenient, and has low LOD value. The method has overcome the shortcomings of the present references. It is easy to apply and has broad clinical application prospect. It lays an experimental foundation for the preparation of Mc Ab against folic acid and the development of domestic kit.

Recovery of Fermented Spinach (<i>Amaranthus</i> sp.) Concentrate Through Ultrafiltration Membrane Process as Source of Folic Acid for Smart Food Formula

Fermentation process on spinach (Amaranthus sp.) by Kombucha culture was done as an effort to recover naturally folic acid as bioactive components to increase smartness. The experimental activity was done by means of UF membrane (100,000 MWCO) fitted in Stirred Ultrafiltration Cell (SUFC) at stirrer rotation speed 200 and 400 rpm, room temperature, pressure 20 and 40 Psi for 30 min. Result of experimental activity showed that based on both selectivity and recovery of folic acid, process optimization of UF was reached at stirrer rotation speed 200 rpm and pressure 40 Psi. In the optimum condition, SUFC technique was able to recover folic acid in retentate 67.75% and in permeate 97.27% (63.19 µg/mL). Identification of monomer in permeate from the optimum process treatment was find out folic acid monomer with molecular weight (MW) 441.39 and relative intensity 93% at mass spectra T2.32 between m/z 257–304 and glutamic acids monomer with MW 148.57 and relative intensity 0.22% at mass spectra T2.82 between m/z 415–470. Other dominant monomer were folic acid fraction.

Folic Acid-fortified Flour: Optimised and Fast Sample Preparation Coupled with a Validated High-Speed Mass Spectrometry Analysis Suitable for a Fortification Monitoring Program

In Australia, folic acid (FA) has been mandatorily added to bread making flour (non-organic wheat flour) since September 2009. The objective of FA fortification is to reduce the prevalence of neural tube defects. This study validated a quantitative assay to determine FA in fortified flour using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). For this purpose, a procedure of aqueous extraction followed by solid phase extraction (SPE) was developed for isolating FA from fortified flour samples. SPE using phenyl cartridges is more selective and avoids the high sodium concentrations used to elute FA from ion exchange sorbents. The validated method is linear over the range 0.024–14 μg/g flour. Absolute recovery of FA was 89 %; inter-day variability coefficient of variation (CV) was 11 % and limit of detection (LOD) was 9.8 ng/g flour. The experimental data revealed that the majority of commercially fortified bread premixes and flour had mean FA concentrations lower than the minimum mandated level of 200 μg/100 g flour. The findings of this study support the need for regular monitoring of actual fortification levels by the Australian States and Territories.

Determination of folic acid in an elemental diet by high-performance liquid chromatography with UV detection

Determination of folic acid (550 ng/g) in an elemental diet containing 46 compounds at concentrations at least 0.02 · 10 6 times higher than that of folic acid was performed by high-performance liquid chromatography with ultraviolet detection at 360 nm using acetonitrile-water (pH 2.1, adjusted with phosphoric acid) (9:91) with the addition of 1 m M ethylenediaminetetraacetic acid disodium salt as the mobile phase. This method is suitable for the determination of folic acid in an elemental diet, because it is simple, rapid, sensitive and reproducible. The calibration graph was linear in the range 0–0.6 μg. Recovery of folic acid was about 95% by the standard addition method. There was good agreement between the indicated and found folic acid concentrations.

Chemical Determination of Folic Acid in the Presence of p-Aminobenzoic Acid or Procaine Hydrochloride

Abstract The USP XVII chemical method for the determination of folic acid is not applicable to pharmaceutical preparations containing p-aminobenzoic acid (PABA) or procaine hydrochloride (added to intertables to reduce discomfort). PABA is often present in older folic acid injectables in which a breakdown of the folic acid has occurred on long standing. A modified method is described to eliminate interference from these compounds by diazotizing the interfering material and then converting it to the corresponding phenol. The folic acid is then cleaved to form PABA, diazotized, and coupled with the Bratton-Marshall reagent. In a comparison of the two methods using known amounts of folic acid in the presence of PABA, better recovery of folic acid was achieved with the modified method than with the USP method. The application of this modification to commercial preparations is also demonstrated.