Recovery Of 15N-labelled Fertilizer Research Articles

Corrigendum to: Early growing season immobilisation affects post-tillering wheat nitrogen uptake from crop stubble and 15N fertiliser in a sandy soil

In semiarid sandy soil environments there is a dual challenge of carbon and nitrogen (N) limitation that needs to be managed to ensure timely supply of N to crops. Management of N inputs to soil using combinations of legume stubble addition and fertiliser N in cereal systems is essential to meet crop demand and maintain N in soil organic matter. The aim of this study was to assess soil mineral and biological N pools that influence N supply and N uptake of wheat at early growth stages. The recovery of 15N-labelled fertiliser by wheat was evaluated using a factorial combination of either wheat, lupin or no stubble incorporated with or without 15N fertiliser in a sandy soil system. Soil and plant samples were collected at sowing, tillering, first node and booting to monitor changes in N pools and 15N uptake by the wheat. Crop stubble incorporation one week before sowing increased biological N pools in the surface soil (0–10 cm). Early N immobilisation (sowing–tillering) in all the treatments without 15N fertiliser may have limited N availability for wheat uptake in the subsequent period (tillering–first node), when fertiliser N appeared critical to maximise N supply for plant requirements. Up to 38% of the 15N fertiliser applied at sowing was incorporated into the soil microbial biomass pool, so that fertiliser N was critical to relieve short-term inherent N limitations for both plant and microbial growth, and to supply the longer-term biological pools (microbial biomass) to support subsequent mineralisation potential. Reducing the energy limitation to the microbial pool through inputs of carbon from stubble was also critical to ensure fertiliser N supplied sufficient N to satisfy plant demand later in the growing period. These results have implications for management decisions on semiarid sandy soil systems that aim to synchronise N from inputs of legume stubbles and fertiliser with crop N demand during early growth stages of wheat.

Early growing season immobilisation affects post-tillering wheat nitrogen uptake from crop stubble and 15N fertiliser in a sandy soil

In semiarid sandy soil environments there is a dual challenge of carbon and nitrogen (N) limitation that needs to be managed to ensure timely supply of N to crops. Management of N inputs to soil using combinations of legume stubble addition and fertiliser N in cereal systems is essential to meet crop demand and maintain N in soil organic matter. The aim of this study was to assess soil mineral and biological N pools that influence N supply and N uptake of wheat at early growth stages. The recovery of 15N-labelled fertiliser by wheat was evaluated using a factorial combination of either wheat, lupin or no stubble incorporated with or without 15N fertiliser in a sandy soil system. Soil and plant samples were collected at sowing, tillering, first node and booting to monitor changes in N pools and 15N uptake by the wheat. Crop stubble incorporation one week before sowing increased biological N pools in the surface soil (0–10 cm). Early N immobilisation (sowing–tillering) in all the treatments without 15N fertiliser may have limited N availability for wheat uptake in the subsequent period (tillering–first node), when fertiliser N appeared critical to maximise N supply for plant requirements. Up to 38% of the 15N fertiliser applied at sowing was incorporated into the soil microbial biomass pool, so that fertiliser N was critical to relieve short-term inherent N limitations for both plant and microbial growth, and to supply the longer-term biological pools (microbial biomass) to support subsequent mineralisation potential. Reducing the energy limitation to the microbial pool through inputs of carbon from stubble was also critical to ensure fertiliser N supplied sufficient N to satisfy plant demand later in the growing period. These results have implications for management decisions on semiarid sandy soil systems that aim to synchronise N from inputs of legume stubbles and fertiliser with crop N demand during early growth stages of wheat.

Wheat response to nitrogen splitting applied to a Vertisols in different tillage systems and cropping rotations under typical Mediterranean climatic conditions

The application of an adequate rate and splitting of nitrogen (N) is essential for the efficient use of N fertiliser and to maintain the economic sustainability of cropping systems. A 3-year field experiment was conducted on a Vertisol under Mediterranean conditions to determine the effect of tillage systems, crop rotation, and variations in N timing on the grain yield and N recovery of 15N-labelled fertiliser (NR) in hard red spring wheat (Triticum aestivum L.). The experiment was designed as a randomised complete block with a split–split plot arrangement and 3 replications. The main plots were tillage system (no-tillage [NT] and conventional tillage [CT]), and the subplots were the preceding crop with 2-year rotations (wheat–sunflower [WS], wheat–chickpea [WC], and wheat–faba bean [WFB]). Sub-subplots were the N timing (all at the same rate of 100kgNha−1), and the fertiliser was applied 50% at sowing and 50% at stem elongation and 50% at tillering and 50% at stem elongation. The area of each basic plot was 50m2 (5m×10m). The residual NO3–N content (0–90cm) was significantly higher in CT than in NT, the opposite occurring with grain yield. The NR of NT was greater than that of CT. According to the previous crop, the NR was WS>WFB=WC. The soil nitrate content was twice as much when the preceding crop was a legume compared with sunflower and the wheat yields were as follows: WFB>WC>WS. Although the N timing did not have an effect on overall grain yield, the interactions with year, tillage system and previous crop were significant. The average recovery of 15N fertiliser by wheat was 44.6%, with 33.7% obtained in the grain (41% in stem elongation, 32% in tillering and 27% in sowing). The habitual use of high rates of N fertiliser and the frequency of dry years in the agrosystem studied generated a progressive accumulation of residual in the soil profile. This can represent an important source of mineral N for the cereal and can reduce the need to apply N fertiliser to the crop.

Nitrogen demand and the recovery of 15N-labelled fertilizer in wheat grown under elevated carbon dioxide in southern Australia

There are few reports on the effects of atmospheric carbon dioxide concentration ([CO2]) on fertilizer N recovery by crops under open-air conditions. This study was conducted at the Australian Grains Free-Air CO2 Enrichment (AGFACE) facility in southern Australia to investigate the effects of elevated [CO2] (550 μmol mol−1) on growth, N uptake and fertilizer 15N recovery by spring wheat (Triticum aestivum L. cv. Yitpi) over a 2-year period. 15N-enriched (10.22 atom%) granular urea was applied to microplots at 50 kg N ha−1 at varying seasonal rainfall and temperature scenarios (simulated by supplementary irrigation and late sowing) for three experimental periods [2008 normal sowing (2008NS), 2008 late sowing (2008LS) and 2009 normal sowing (2009NS)]. Elevated [CO2] increased wheat biomass (27–58%), N uptake (18–44%) and amount of plant N derived from soil (20–50%) at 2008NS and 2009NS (rainfed), but the effect was not apparent at 2008LS (hotter and drier) and supplementary irrigated plots for 2009NS (above-average rainfall). Tissue N concentration and N derived from fertilizer were unaffected by elevated [CO2] in any experimental period. Irrespective of [CO2], grain yield and whole plant fertilizer N uptake was 37–94 and 13–609%, respectively, higher under supplementary irrigated plots than that in rainfed counterparts. These results indicate that more fertilizer N will need to be applied to this wheat production zone under future [CO2] environments, and yield gains in hotter and drier climates will be lower than those in higher rainfall zones.

Recovery of 15N-labelled fertilizer applied to winter wheat and perennial ryegrass crops and residual 15N recovery by succeeding wheat crops under different crop residue management practices

The recovery of 15N-labelled fertilizer applied to a winter wheat (120 kg N ha−1) and also a perennial ryegrass (60 kg N ha−1) crop grown for seed for 1 year in the Canterbury region of New Zealand in the 1993/94 season was studied in the field. After harvests, ryegrass and wheat residues were subjected to four different residue management practices (i.e. ploughed, rotary hoed, mulched and burned) and three subsequent wheat crops were grown, the first succeeding wheat crop sown in 1994/95 to examine the effects of different crop residue management practices on the residual 15N recovery by succeeding wheat crops. Total 15N recoveries by the winter wheat and ryegrass (seed, roots and tops) were 52% and 41%, respectively. Corresponding losses of 15N from the crop-soil systems represented by un-recovered 15N in crop and soil were 12% and 35%, respectively. These losses were attributed to leaching and denitrification. The proportions of 15N retained in the soil (0-400 mm depth) at the time of harvest of winter wheat and ryegrass were 36% and 24%, respectively. Although the soil functioned as a substantial sink for fertilizer N, the recovery of this residual fertilizer by subsequent three winter wheat crops was low (1-5%) and this was not affected by different crop residue management practices.

Recovery of 15N-labelled fertiliser by a perennial ryegrass seed crop and a subsequent wheat crop

Large amounts of nitrogen (N) fertiliser (150–200 kg N/ha) are currently being applied to perennial ryegrass (Lolium perenneL.) seed crops in New Zealand. Due to increasing requirements for efficient use of N fertilisers and minimising nitrate contamination of the environment, a field experiment was established using 15N-labelled fertiliser to follow the fate of applied N. Urea-15N was applied to a perennial ryegrass seed crop in April (30 kg N/ha), August (30 kg N/ha), September (60 kg N/ha) and October (60 kg N/ha). The urea-15N was applied in solution and watered in to minimise volatilisation loss. At the time of harvest (December), 9% of the applied 15N was in the seed, 29% in the straw, 19% in the roots and 39% in the soil organic matter. Losses of 15N were minimal as the N was applied in several applications, each one at a relatively low rate, and at times when leaching was unlikely to occur. Ryegrass plants used a greater proportion of the N applied in September and October (61–65%) compared with that applied in April (44%). Consequently more N was recovered from the soil in the autumn application (57%) than from the September and October applications (28–44%). The availability of the residual fertiliser N to a subsequent wheat (Triticum aestivum L.) crop was studied in a glasshouse experiment. The residual fertiliser N was present in the soil and ryegrass roots and stubble. The wheat plants only recovered 7–9% of this residual N. Most of the N taken up by the wheat came from the soil organic N pool. Overall, applying a total of 180 kg N/ha to the ryegrass appeared to have minimal direct impact on the environment. In the short term N not used by the ryegrass plants contributed to the soil organic N pool.

Variation in N-recovery of winter wheat under Mediterranean conditions studied with 15N-labelled fertilizers

We studied the fate of 15N-labelled fertilizers applied to winter wheat ( Triticum aestivum L., cv. Tejo) grown for 3 years on a Haplic Luvisol in southern Portugal. The nitrogen rate used was 60 kg ha −1 using ammonium nitrate as basal dressing, and two top-dressings of 60 kg ha −1 of N each, either as urea or calcium nitrate. Microplots (PVC cylinders with 30 cm diameter) with ceramic cups placed at the bottom were used, so that leachates could be collected by hand pumping. A greater percentage of plant N was derived from the fertilizer in the driest year (year 1) than in the other two years. However, biomass accumulation was smaller in that year and, therefore, plants took up less N. As a result, the recovery of 15N-labelled fertilizer in the crop was in the range of 22–40%, with no significant differences between years or treatments. Considerable amounts of N derived from fertilizer remained in the soil after harvest (15 to 66% of total fertilizer N). In years 1 and 2, the 15N in the soil derived from fertilizer was similar, and not significantly affected by N form. In year 3, top-dressing with urea led to more of the 15N from fertilizer remaining in the soil, compared with applying nitrate, and to greater values of total soil N, nitrate and extractable ammonium. There was evidence of nitrate leaching in years 2 and 3, particularly after the first two N applications, during the autumn–winter period, but not significantly affected by treatment. A significant amount of 15N remaining in the soil was in 15 NH + 4 fixed in the clay fraction, especially in the surface layers and with urea application, suggesting that ammonium fixation in montmorillonite was an important process for removing NH + 4 from solution and protection from nitrification. N losses were similar (35 to 55%) in years 1 and 2 with both treatments, and in year 3 with ammonium nitrate+calcium nitrate application. In year 3 the ammonium nitrate+urea treatment resulted in a complete recovery of 15N applied.

Growth response to fertilisation and recovery of 15N-labelled fertiliser by young hoop pine plantations of subtropical Australia

The stand growth responses to fertilisation were investigated in 1-year-old and 5-year- old second rotation (2R) hoop pine (Araucaria cunninghamii) plantations in subtropical Australia. At the 1-year-old plantation, 4 rates of nitrogen (N) fertiliser (0, 20, 60 and 120 kg N ha-1) were banded either with or without basal fertilisers (BF) containing 60 kg P ha-1 and 50 kg K ha-1. In the 5-year-old plantation, 4 rates of N fertiliser (0, 100, 300, 600 kg N ha-1) were banded with or without the BF. At both sites N fertiliser was applied as ammonium sulphate in 4 equal split dressings over 2 growing seasons. 15N-labelled ammonium sulphate was also used at the 5-year-old plantation in conjunction with the 100 kg N ha-1 treatment. The 15N-labelled fertiliser was applied to 3 trees during the first split application in spring, and to 3 more trees during the second split application in midsummer. The 15N-labelled trees were harvested and the surrounding soil excavated 6 months after the second 15N application. In the 1-year-old plantation, no stand growth response to fertilisation was noted, indicating that fertilisation at plantation establishment was unlikely to increase plantation productivity. In contrast, a significant stand response to N fertilisation was demonstrated at the 5-year-old site. There were no significant differences between the plus N treatments and the increase in basal area and volume due to N fertilisation 2.3 years after commencement of the trial was 45.1 and 43.5%, respectively. No response to basal fertiliser was observed. The mean 15N recovery from the soil–plant system was 79%, with no significant difference noted between the two split applications. The mean 15N recovery in the tree biomass was 56%, with the majority being partitioned into the foliage. On average 21% of the applied 15N could not be accounted for, and was assumed to be lost from the soil–plant ecosystem.

Effect of fertilizer rate and form on the recovery of 15N-labelled fertilizer applied to wheat in Syria

15N-labelled fertilizer was applied at different rates (0, 30, 60, 90 kg N ha−1) and in different forms (urea or ammonium sulphate) to wheat grown in Syria in three seasons (1991/92, 1992/93 and 1994/95).Recovery of 15N-labelled fertilizer in the above-ground crop at harvest was low (8–22%), with the amount of 15N-labelled fertilizer recovered in the crop increasing as the rate of application increased. Fertilizer application caused a significant increase in the amount of unlabelled soil N in the crop, suggesting that the application of N fertilizer caused a ‘real’ added nitrogen interaction. Recovery of 15N-labelled fertilizer in the crop was unaffected by the form of the fertilizer.On average 31% (14–54%) of the 15N-labelled fertilizer remained in the soil at harvest, mostly in the 0–20 cm layer. At the lowest application rate (30 kg N ha−1) most of the residual fertilizer was as organic N, but at the higher application rates (60 and 90 kg N ha−1), a greater proportion of the 15N-labelled fertilizer was recovered as inorganic N, presumably as the result of top-dressing N in dry conditions in the spring. The amount of 15N-labelled fertilizer remaining in the soil increased as the fertilizer rate increased, but was unaffected by the form of fertilizer applied.Losses of 15N-labelled fertilizer were large (>35%), probably caused by gaseous losses, either through volatilization of N from the calcareous soil, or through denitrification from wet soils rich in organic residues.N fertilization strategies in the West Asia/North Africa (WANA) region should take note of the low recovery of N fertilizer by the crop in the season of application, and the resultant large quantities of residual fertilizer.

The effect of rate, form and timing of fertilizer N on nitrogen uptake and grain N content in spring malting barley

SUMMARYField experiments were carried out on six sites in eastern Scotland between 1987 and 1989 to determine the effect of nitrogen on the yield, N uptake and grain N concentration of spring barley grown for malting. The effects of fertilizer applications at rates from 0 to 150 kg N ha-1 and the timing of application were studied, using three fertilizer forms: calcium nitrate, ammonium sulphate and ammonium nitrate. Calcium nitrate applications significantly increased grain N concentrations (P < 0·05), and grain yields (P < 0·01 and 0·05) at two sites, above the values obtained with the other fertilizers, but there was no effect at the other sites. Split applications of calcium nitrate increased yields above those from single applications in some treatments at two sites. At low rates, recovery of 15N-labelled fertilizer was greatest when applied as calcium nitrate. Recovery fell at higher rates in calcium nitrate treatments, but rose in ammonium sulphate treatments. Uptake of fertilizer N, during the period of stem elongation in June, was significantly greater (P < 0·05) in the calcium nitrate and ammonium nitrate treatments. Maximum uptake was usually reached by the time of anthesis. Uptake of soil N was not as great during the early sampling periods, but continued up to harvest in most treatments. There was evidence of losses, between anthesis and harvest, of fertilizer N previously taken up by the crop. The uptake of soil N remained constant over the range of fertilizer treatments except with ammonium sulphate, where there was evidence of increased uptake at higher fertilizer rates, possibly due to ‘pool substitution’ of 15N-labelled fertilizer. The variation in soil N uptake between sites was greater than the variation in fertilizer N uptake caused by different forms of fertilizer and different application times.

Recovery of15N-labelled fertilizer by Coastal bermudagrass in lignite minesoil

Minesoils developed from lignite surface mining in Texas are nutrient-poor and have a high N retention capacity. A major concern of landowners and soil conservationists is the response of Coastal bermudagrass to the application of low rates of ammonium-N fertilizer on these nutrient-poor minesoils. A glasshouse study, using15N-labelled ammonium sulfate fertilizer and lignite minesoil, was conducted to measure Coastal bermudagrass biomass production and fertilizer recovery during establishment in response to clipping at 2, 4, and 8 week intervals. At N rates of 0, 40, and 80 kg N ha−1,increases in N fertilization increased Coastal bermudagrass aboveground biomass 5-fold, but showed only small increases in belowground biomass. Recovery of ammonium-N fertilizer ranged from 54 to 63%. Roots contained approximately the same N content across all fertilizer rates suggesting that young, estabilishing, Coatal bermudagrass roots reserve N until their N requirement is met. As more N is obtained above that which was needed to maintain roots, then additional N taken up by the plant was transported to aboveground plant parts for growth. Frequent clipping intensified N transport to aboveground tissues. Reduced amounts of N were contained in roots after clipping due to reductions in root growth, biomass, and resource demand. Fertilization of Coastal bermudagrass at low N rates with different N fertilizer forms influenced the distribution of N in the plant and affected N recovery by different parts of the plant.

Recovery of15N-labelled fertilizer by sugar beet?spring wheat and winter rye?sugar beet cropping sequences

The recovery of15N labelled ammonium fertilizer was studied during two cropping sequences: sugar beet—spring wheat and winter rye—sugar beet with the labelled N applied to the first crop of each sequence. The difference between fall and spring application was also investigated. For the first cropping sequence 100 kg N ha−1 labelled with 11.4%15N atom excess (a.e.) was applied to the sugar beets. This labelled N was followed in the sugar beets, in the soil profile at harvesting time as well as in the spring wheat of the following year. The first crop of sugar beet recovered 43–46% of the applied N, with 26–29% remaining in the soil at harvesting time and 25–31% could not be accounted for. Of the residual N, less than 1% could be recovered by the next crop of spring wheat. For the second cropping sequence 50 kg N ha−1 labelled with 11.5%15N a.e. was applied to the winter rye and followed in the winter rye and in the sugar beets of the following year. The recovery of the labelled fertilizer N applied to the winter rye of the second sequence was 20–27% and the sugar beets of the next year could only recover 2%. With respect to time of application, no difference in fertilizer N recovery was found between fall or spring application for the two sequences.