A field experiment was conducted at the Research Farm of Dr. Rajendra Prasad Central Agricultural University, Pusa, Bihar, to devise nutrient management strategies aimed at sustaining soil health, quality and sugarcane production within the sugarcane plant-ratoon system. The experiment evaluated the efficacy of various fertilizers on the solubility of applied inorganic fertilizer during the spring season in calcareous soil. Results from the combined data analysis showed significant variations in the number of millable cane (NMC), cane yield, and sugar yield due to the integrated application of organic and inorganic fertilizers along with bio-fertilizers in both the plant and ratoon crops. Treatments combining organic and inorganic fertilizers with bio-fertilizers demonstrated a noteworthy increase in NMC, cane yield, and sugar yield compared to the control group. The treatment that received 75% NPK of recommended dose of fertilizer (RDF) along with Acetobacter, PSB, and Bio-compost at a rate of 7.5 t ha-1 exhibited the highest NMC (103.0 × 103 ha-1), cane yield (85.8 t ha-1), and sugar yield (11.21 t ha-1). Furthermore, the residual effect of the treatment combining organic and inorganic fertilizers with bio-fertilizers showed a pronounced impact on NMC (92.4 × 103 ha-1), yield (79.6 t ha-1), and sugar yield (9.36 t ha-1) in the ratoon crop under treatment T9. Bio-compost notably enhanced the overall performance of sugarcane. Nutrient uptake by both plant and ratoon followed a similar trend as cane yield. Application of RDF alongside various bio-fertilizers significantly enhanced sugarcane productivity compared to the control group. Notably, the efficacy of bio-fertilizers was more pronounced when used in conjunction with inorganic treatments. Treatment with organic inputs through bio-compost resulted in reduced pH and increased electrical conductivity (EC), organic carbon, and available nutrients (N,P, and K) in post-harvest soil. Enzymatic activities including glycosidase, urease, acid phosphatase, and dehydrogenase were also recorded.