Vibration signals from rotating and reciprocating machines provide insights into machine health and operating parameters. Reciprocating machines often feature cylinder deactivation systems for increased efficiency. This article presents a vibration-based method for the detection of activated cylinders in reciprocating machines. Simulated compressor vibration signals demonstrate the effect of cylinder conditions on spectral-coherence and mean-envelope-spectrum (MES) estimates. Physical parameters of the mechanical system and the results of the simulations advise the proposed method. With an appropriately chosen region of interest (ROI), the MES-based indicator (MESbI) correctly identified the number of activated cylinders of a four-cylinder reciprocating refrigeration compressor in both field and laboratory test setups. A discussion section addresses measurement uncertainty and calibration, and provides an example application.