The diameter and weight of different brands of table tennis ball will affect the ball’s elasticity and stroke. The purpose of this study is to analyze the difference of the dynamic elasticity and stroke effect between the two brands of new plastic ball. A self-made experiment was designed to test the dynamic elasticity characteristics of DHS D40 + and Nittaku 40+. Table tennis players (N = 18) were randomly selected from the China Table Tennis College (Mage = 15.16 ± 2.41; Mheight = 1.59 ± 0.32 m; Mweight = 45.72 ± 5.17 kg). Each participant was righthand shake-hands grip. A speedometer was used to record the ball speed and a high-speed camera was used to measure the spin speed. Data normality was verified by using the Kolmogorov-Smirnov test. The independent t-test was conducted to assess the differences of the dynamic elasticity and stroke effect between the two types of plastic ball. Results showed that the rebound speed and decrement rate of DHS D40 + and Nittaku 40 + both increased with the increased falling speed, respectively. When falling at high speed, there was a significant difference of dynamic elasticity between DHS D40 + and Nittaku 40+ (p < 0.01). There was also a significant difference in the ball speed and spin speed between the two types of new material seamed plastic ball during the backhand backspin stroke (p = 0.041, p = 0.022, respectively), and the ball speed and spin speed of DHS D40 + were higher than that of Nittaku 40 + ball. Compared with the DHS D40+, the Nittaku 40 + has a faster rebound speed, higher rebound height, and better dynamic elasticity. Therefore, under same striking conditions, when hitting the Nittaku 40 + ball, players need to increase the swing distance and hit the ball with more strength to improve the ball speed and rotation speed; increase the spin and decrease the ball’s rebound height of the serve.