Change in design of chassis, brings in (i) change in vibration pattern and (ii) stress pattern will also change for impact loading conditions. This paper aims to study the vibration characteristics and structural behaviour of three different Go-Kart chassis configurations. Chassis configurations are designed as per standards followed by major Go-Kart events organized in India and abroad. To study the vibration aspects, with change in design, modal analysis is done. Front, side and rear impact simulations are carried out to obtain displacement and stress distribution patterns. Three-dimensional CAD model is designed using SOLIDWORKS. Finite Element Analysis (FEA) software ANSYS Ver 16.0 is used to determine the Natural frequencies and impact testing. AISI 1018 material is taken for all the simulation cases. 4-G load is applied for front and rear impact while 2-G is applied for side impact test simulation. Results are extracted in terms of Natural Frequencies, von-Mises stress and deformation for all the simulation cases. Design 1 is found to have good vibration characteristics and is also possessing a higher degree of safety. The obtained results will be useful for students working to build their vehicle as well as industry people to fabricate safer and reliable Go-Kart vehicles.