Rates Of Biogeochemical Processes Research Articles

A protocol for the synthesis of [35S]-labeled 3-dimethylsulfoniopropionate and dimethylsulfide from L-methionine for use in biogeochemical studies

Radiotracers are highly sensitive tools for quantifying the rates of important biogeochemical processes and the fates of specific atoms and/or compounds within major global elemental cycles, especially those that are requisite for life. Important radiolabeled organosulfur compounds, like dimethylsulfide (DMS) and its precursor 3-dimethylsulfoniopropionate (DMSP), are not commercially available, but their well-documented use has been key in furthering our understanding of the marine sulfur cycle. [35S]-DMSP obtained by chemical synthesis has been used extensively in radiotracer studies involving DMS and DMSP, but its synthesis has been restricted to 2 research groups. Presented here is a protocol for the chemical synthesis of [35S]-DMSP from [35S]-L-methionine, though the method could be used for other radiolabels (e.g. [14C], [3H]). The synthesis consists of 2 reaction steps, (1) the sequential oxidative deamination and decarboxylation of [35S]-L-methionine to [35S]-3-methylmercaptopropionate and (2) the methylation of [35S]-methylmercaptopropionate to yield the product [35S]-DMSP. The product is purified by liquid chromatography and two cation-resin exchanges. Average final [35S]-DMSP yield was 5.34% (n = 16; range: 1.26% to 14.84%, excluding failures), although updated instrumentation could likely improve final yields. The objective of this work is to standardize the synthesis of [35S]-DMSP to widen its availability and use among the community and hence facilitate increased understanding of the reduced sulfur and carbon cycles.

Distribution of nutrients and dissolved organic matter in a eutrophic equatorial estuary: the Johor River and the East Johor Strait

Abstract. Estuaries have strong physicochemical gradients that lead to complex variability and often high rates of biogeochemical processes, and they are also often impacted by humans. Yet, our understanding of estuarine biogeochemistry remains skewed towards temperate latitudes. We examined seasonal and spatial variability in dissolved organic matter (DOM) and nutrients along a partly eutrophic, agricultural–urban estuary system in Southeast Asia: the Johor River and the East Johor Strait. Dissolved organic carbon (DOC) and coloured DOM (CDOM) showed non-conservative mixing, indicating significant DOM inputs along the estuary. The CDOM spectral slopes and CDOM : DOC ratios suggest that terrigenous, soil-derived DOM dominates along the Johor River, while phytoplankton production and microbial recycling are important DOM sources in the Johor Strait. CDOM properties were not unambiguous source indicators in the eutrophic Johor Strait, which is likely due to heterotrophic CDOM production. Nitrate concentrations showed conservative mixing, while nitrite concentrations peaked at intermediate salinities of 10–25. Ammonium concentrations decreased with salinity in the Johor River but increased up to 50 µmol L−1 in the Johor Strait, often dominating the dissolved inorganic nitrogen (DIN) pool. Phosphate concentrations were low (<0.5 µmol L−1) throughout the Johor River but increased in the Johor Strait, where DIN : phosphate ratios were typically ≥ 16 : 1. This suggests that the Johor Strait may experience phosphorus limitation and that internal recycling is likely important for maintaining high nutrient concentrations in the Johor Strait. Overall, our results indicate that the Johor River and Johor Strait are clearly not part of the same estuarine mixing continuum and that nutrient recycling processes must be quantified to understand nutrient dynamics in the Johor Strait. Moreover, our results highlight the need for better techniques for DOM source tracing in eutrophic estuaries.

Balance and imbalance in biogeochemical cycles reflect the operation of closed, exchange, and open sets

Biogeochemical reactions modulate the chemical composition of the oceans and atmosphere, providing feedbacks that sustain planetary habitability over geological time. Here, we mathematically evaluate a suite of biogeochemical processes to identify combinations of reactions that stabilize atmospheric carbon dioxide by balancing fluxes of chemical species among the ocean, atmosphere, and geosphere. Unlike prior modeling efforts, this approach does not prescribe functional relationships between the rates of biogeochemical processes and environmental conditions. Our agnostic framework generates three types of stable reaction combinations: closed sets, where sources and sinks mutually cancel for all chemical reservoirs; exchange sets, where constant ocean-atmosphere conditions are maintained through the growth or destruction of crustal reservoirs; and open sets, where balance in alkalinity and carbon fluxes is accommodated by changes in other chemical components of seawater or the atmosphere. These three modes of operation have different characteristic timescales and may leave distinct evidence in the rock record. To provide a practical example of this theoretical framework, we applied the model to recast existing hypotheses for Cenozoic climate change based on feedbacks or shared forcing mechanisms. Overall, this work provides a systematic and simplified conceptual framework for understanding the function and evolution of global biogeochemical cycles.

River water temperature demonstrates resistance to long‐term air temperature change

AbstractEcosystem health and water quality of rivers are dependent on their temperature. With ongoing human‐induced climate change causing increases in air temperature across the globe, it is anticipated the stream temperatures will rise too—in turn increasing the rates of biogeochemical stream processes and potentially threatening the viability and health of aquatic organisms. To understand the relationship between climate change and stream temperature response, the longer the records that can be analysed, the more the robust the analysis for detecting change. In this study, we analyse records from 263 catchments from across the United Kingdom for 45 years from 1974 to 2019 to assess the link between air temperature and stream temperature change. To give the most precise analysis of these long records, Bayesian hierarchical modelling was used and showed that: (i) The Bayesian hierarchical approach was 59% more precise, that is, reduced uncertainty on long‐term trends, than using simple linear regression. (ii) The increase in annual average air temperature over 45 years across the United Kingdom showed no significant differences between 22 weather stations and gave a 45‐year change of 1.35 ± 0.9°C. (iii) Trends in annual mean stream temperature change varied from −2.3 °C to 2.0 °C over 45 years, with the mean over 263 sites being 0.5 °C over 45 years. (iv) 1% of rivers showed a stream temperature trend significantly greater than the air temperature trend but 3% of sites showed a stream temperature trend significantly lower than zero. (v) 74% of all river sites showed no significant monotonic trend, either positive or negative, in water temperature even after 45 years. The observed declines in stream temperature could be ascribed to the closures of thermoelectric power stations but it is unclear why the stream temperature at some sites has risen faster than air temperature. The study shows that mean river temperature was well buffered against changes in air temperature—a 1°C rise in air temperature giving 0.37°C in mean stream temperature.

Superlinear scaling of riverine biogeochemical function with watershed size

River networks regulate carbon and nutrient exchange between continents, atmosphere, and oceans. However, contributions of riverine processing are poorly constrained at continental scales. Scaling relationships of cumulative biogeochemical function with watershed size (allometric scaling) provide an approach for quantifying the contributions of fluvial networks in the Earth system. Here we show that allometric scaling of cumulative riverine function with watershed area ranges from linear to superlinear, with scaling exponents constrained by network shape, hydrological conditions, and biogeochemical process rates. Allometric scaling is superlinear for processes that are largely independent of substrate concentration (e.g., gross primary production) due to superlinear scaling of river network surface area with watershed area. Allometric scaling for typically substrate-limited processes (e.g., denitrification) is linear in river networks with high biogeochemical activity or low river discharge but becomes increasingly superlinear under lower biogeochemical activity or high discharge, conditions that are widely prevalent in river networks. The frequent occurrence of superlinear scaling indicates that biogeochemical activity in large rivers contributes disproportionately to the function of river networks in the Earth system.

Microbial Communities Involved in Methane, Sulfur, and Nitrogen Cycling in the Sediments of the Barents Sea.

A combination of physicochemical and radiotracer analysis, high-throughput sequencing of the 16S rRNA, and particulate methane monooxygenase subunit A (pmoA) genes was used to link a microbial community profile with methane, sulfur, and nitrogen cycling processes. The objects of study were surface sediments sampled at five stations in the northern part of the Barents Sea. The methane content in the upper layers (0–5 cm) ranged from 0.2 to 2.4 µM and increased with depth (16–19 cm) to 9.5 µM. The rate of methane oxidation in the oxic upper layers varied from 2 to 23 nmol CH4 L−1 day−1 and decreased to 0.3 nmol L−1 day−1 in the anoxic zone at a depth of 16–19 cm. Sulfate reduction rates were much higher, from 0.3 to 2.8 µmol L−1 day−1. In the surface sediments, ammonia-oxidizing Nitrosopumilaceae were abundant; the subsequent oxidation of nitrite to nitrate can be carried out by Nitrospira sp. Aerobic methane oxidation could be performed by uncultured deep-sea cluster 3 of gamma-proteobacterial methanotrophs. Undetectable low levels of methanogenesis were consistent with a near complete absence of methanogens. Anaerobic methane oxidation in the deeper sediments was likely performed by ANME-2a-2b and ANME-2c archaea in consortium with sulfate-reducing Desulfobacterota. Sulfide can be oxidized by nitrate-reducing Sulfurovum sp. Thus, the sulfur cycle was linked with the anaerobic oxidation of methane and the nitrogen cycle, which included the oxidation of ammonium to nitrate in the oxic zone and denitrification coupled to the oxidation of sulfide in the deeper sediments. Methane concentrations and rates of microbial biogeochemical processes in sediments in the northern part of the Barents Sea were noticeably higher than in oligotrophic areas of the Arctic Ocean, indicating that an increase in methane concentration significantly activates microbial processes.

Capillary absorption spectroscopy for high temporal resolution measurements of stable carbon isotopes in soil and plant-based systems

Capillary Absorption Spectroscopy (CAS) is a relatively new analytical technique for performing stable isotope analysis. Here, we demonstrate the utility of CAS by recording and quantifying variation in 13C in controlled and biologically relevant applications. We calibrated CAS system response to increased 13CO2, with an observed ∼4‰ increase in measured Δ13C for each 0.03 ppm shift in 13CO2 concentration. We leveraged this calibration to quantify rates of biogeochemical processes using a 13C tracer. For example, we monitored microbial respiration of 13C-glucose within an agricultural soil at 10 s quantification intervals and results demonstrated 8.6% ± 0.4 of added glucose was converted to 13CO2 within 1.5 h of incubation. We expanded the demonstration by adapting a rhizobox to permit continuous monitoring of 13CO2 in a soil (as distinct from plant) headspace to track the timing and quantify respiration rates of fresh plant photosynthate and observed a 3.5 h delay between plant exposure to a13CO2 tracer and the first signs of respiration by soil biota. These experiments highlight CAS is effective in producing high temporal resolution quantification of 13CO2 and demonstrate potential applications.

Effectiveness of phosphorus control under extreme heatwaves: implications for sediment nutrient releases and greenhouse gas emissions

Eutrophication has been identified as the primary cause of water quality deterioration in inland waters worldwide, often associated with algal blooms or fish kills. Eutrophication can be controlled through watershed management and in-lake measures. An extreme heatwave event, through its impact on mineralization rates and internal nutrient loading (phosphorus—P, and nitrogen—N), could counteract eutrophication control measures. We investigated how the effectiveness of a nutrient abatement technique is impacted by an extreme heatwave, and to what extent biogeochemical processes are modulated by exposure to heatwaves. To this end, we carried out a sediment-incubation experiment, testing the effectiveness of lanthanum-modified bentonite (LMB) in reducing nutrients and greenhouse gas emissions from eutrophic sediments, with and without exposure to an extreme heatwave. Our results indicate that the effectiveness of LMB may be compromised upon exposure to an extreme heatwave event. This was evidenced by an increase in concentration of 0.08 ± 0.03 mg P/L with an overlying water volume of 863 ± 21 mL, equalling an 11% increase, with effects lasting to the end of the experiment. LMB application generally showed no effect on nitrogen species, while the heatwave stimulated nitrification, resulting in ammonium loss and accumulation of dissolved oxidized nitrogen species as well as increased dissolved nitrous oxide concentrations. In addition, carbon dioxide (CO2)-equivalent was more than doubled during the heatwave relative to the reference temperature, and LMB application had no effect on mitigating them. Our sediment incubation experiment indicates that the rates of biogeochemical processes can be significantly accelerated upon heatwave exposure, resulting in a change in fluxes of nutrient and greenhouse gas between sediment and water. The current efforts in eutrophication control will face more challenges under future climate scenarios with more frequent and intense extreme events as predicted by the IPCC.

Metagenomic reconstruction of nitrogen and carbon cycling pathways in forest soil: Influence of different hardwood tree species

The soil microbiome plays an essential role in processing and storage of nitrogen (N) and carbon (C) and is influenced by vegetation above-ground through imparted differences in chemistry, structure, mass of plant litter, root physiology, and dominant mycorrhizal associations. We used shotgun metagenomic sequencing to quantify the abundance and distribution of gene families involved in soil microbial N and C cycling beneath three deciduous hardwood tree species: ectomycorrhizal (ECM)-associated Quercus rubra (red oak), ECM-associated Castanea dentata (American chestnut), and arbuscular mycorrhizal (AM)-associated Prunus serotina (black cherry). Chestnut exhibited the most distinct soil microbiome of the three species, both functionally and taxonomically, with a general suppression of functional genes in the nitrification, denitrification, and nitrate reduction pathways. These changes were related to low inorganic N availability in chestnut stands as soil was modified by poor, low-N litter quality relative to red oak and black cherry soils. Previous studies have used field biogeochemical process rates, isotopic tracing, and targeted gene abundance measurements to study the influence of tree species on ecosystem N and C dynamics. However, these approaches do not enable a comprehensive systems-level understanding of the relationship between microbial diversity and dynamics of N and C below-ground. We analyzed microbial metagenomes from soils beneath red oak, American chestnut, and black cherry stands and showed that tree species can mediate the abundance of key microbial genes involved in N and (to a lesser extent) C metabolism pathways in soil. We compare gene abundance values to measures of N and C flux from incubated soil. Our results highlight the genetic framework underlying tree species’ control over soil microbial communities, and below-ground C and N metabolism, and may enable land managers to select tree species to maximize C and N storage in soils.

Faunal and environmental drivers of carbon and nitrogen cycling along a permeability gradient in shallow North Sea sediments

Ecosystem functions are driven by abiotic and biotic factors, but due to high collinearity of both, it is often difficult to disentangle the drivers of these ecosystem functions. We studied sedimentological and faunal controls of benthic organic matter mineralization, a crucial ecosystem process provided for by sediments of shelf seas. Subtidal benthic habitats representative of the wide permeability gradient found in the Belgian Part of the North Sea (Northeast Atlantic Shelf) were characterized in terms of sediment descriptors, macrofauna, and sediment biogeochemistry was estimated. Our results confirmed a strong correlation between sediment characteristics and macrofauna, and estimated sediment biogeochemical process rates were clearly linked to both. Results of variance partitioning and statistical modelling showed that oxic mineralization and nitrification were mainly regulated by faunal activities whereas anoxic mineralization was regulated by sediment properties, with permeability as a decisive factor. Both biotic and abiotic factors were needed to explain variability in oxygen consumption and total mineralization estimates, suggesting that macrofaunal activities have different effects across habitats. The statistical models were a useful tool to interpret the impact of anthropogenic activities in the study area and represent a step towards predicting the effects of human activities on crucial ecosystem functions.

A new method to extract 232Th, 230Th and 231Pa from seawater using a bulk-extraction technique with Nobias PA-1 chelating resin

The long-lived radioisotopes of Th and Pa are unique tracers for quantifying rates of biogeochemical processes in the ocean. However, their generally low concentrations (sub-fg/kg for 230Th and 231Pa and pg/kg for 232Th) in seawater make them difficult to measure. Here, we present a new approach to determine 232Th and 230Th using Nobias PA-1 chelating resin following a bulk-extraction technique, and report for the first time the use of this resin to measure 231Pa concentrations. This method has high extraction efficiency (>80%) at pH of 4.4 ± 0.2 and the lowest procedural blanks reported in the literature: 1.0 ± 0.2 pg, 0.10 ± 0.03 fg, and 0.02 ± 0.01 fg for 232Th, 230Th, and 231Pa, respectively, representing 3%, 0.02%, and 0.01% of the total dissolved 232Th, 230Th, and 231Pa found in 5 L of a typical low-concentration surface seawater sample from the subtropical Pacific Ocean. The procedure yields data with high precision for all three isotopes (0.76% for 232Th, 0.89% for 230Th, and 0.96% for 231Pa, 2σ), allowing us to reliably measure Th and Pa in the oceans even at concentrations as low as those found in surface waters of the South Pacific Ocean. The accuracy of this method was confirmed by the analysis of well-characterized standard solutions (SW STD 2010-1 and SW STD 2015-1) and seawater samples collected aboard the FS Sonne (cruise SO245) during the UltraPac cruise in the South Pacific Ocean. Simultaneous and rapid extraction of 232Th, 230Th and 231Pa from seawater, as well as the high precision and accuracy of this method makes it ideal for both spatially and temporally high-resolution studies.

A comprehensive global oceanic dataset of helium isotope and tritium measurements

Abstract. Tritium and helium isotope data provide key information on ocean circulation, ventilation, and mixing, as well as the rates of biogeochemical processes and deep-ocean hydrothermal processes. We present here global oceanic datasets of tritium and helium isotope measurements made by numerous researchers and laboratories over a period exceeding 60 years. The dataset's DOI is https://doi.org/10.25921/c1sn-9631, and the data are available at https://www.nodc.noaa.gov/ocads/data/0176626.xml (last access: 15 March 2019) or alternately http://odv.awi.de/data/ocean/jenkins-tritium-helium-data-compilation/ (last access: 13 March 2019) and includes approximately 60 000 valid tritium measurements, 63 000 valid helium isotope determinations, 57 000 dissolved helium concentrations, and 34 000 dissolved neon concentrations. Some quality control has been applied in that questionable data have been flagged and clearly compromised data excluded entirely. Appropriate metadata have been included, including geographic location, date, and sample depth. When available, we include water temperature, salinity, and dissolved oxygen. Data quality flags and data originator information (including methodology) are also included. This paper provides an introduction to the dataset along with some discussion of its broader qualities and graphics.

A Review on the Distribution and Cycling of Mercury in the Pacific Ocean.

With the rapid development of economy in surrounding land, the Pacific Ocean is facing a number of serious environmental challenges, including mercury (Hg) pollution. Over the past several decades, a number of studies have been conducted on investigating the cycling of Hg in this ecosystem. This review summarizes recent studies on the distribution of Hg species in the water, sediment, and biota and the important processes controlling Hg cycling in the Pacific Ocean. Although a lot of studies have been conducted in this system, more efforts should be made on Hg speciation and cycling in the Pacific Ocean, especially some areas that have rarely studied so far. There is a need to measure the rates of important biogeochemical processes in this ecosystem. Application of multiple methods expected to give a better estimation of the sources and sinks of Hg species in the Pacific Ocean in future studies.

Burrowing macroinvertebrates alter phosphorus dynamics in drainage ditch sediments

Consumptive and nonconsumptive interactions of benthic organisms play important roles in regulating rates of ecosystem services such as nutrient cycling in freshwater ecosystems. Studies of macroinvertebrate communities in drainage ditches have focused on documenting the biodiversity supported by these human-altered environments, but none have explored the ecosystem functions provided by those biological communities in ditches. Bioturbation by burrowing benthic invertebrates in ditch sediments may change rates of biogeochemical processes controlling fluxes of nutrients across the sediment–water interface. We used microcosms to test the effect of four species of burrowing invertebrates (Naididae: Ilyodrilus templetoni, Naididae: Limnodrilus hoffmeisteri, Gammaridae: Crangonyx sp., Chironomidae: Chironomus decorus S.G.) on exchanges of phosphorus between sediment and water from a drainage ditch. These effects were measured across a range of sediment and water characteristics, representing variability within ditches. All species reduced concentrations of P (as molybdenum-reactive phosphorus) in the surface water relative to controls under conditions were sediment porewater was not likely to contain higher concentrations of P than surface water. Decreases in P concentration were linked to changes in the sediment redox potential and water pH. Two species (L. hoffmeisteri and C. decorus) increased P concentrations under conditions where sediment porewater likely had higher concentrations of P than surface water. Increases in P concentrations were likely due to physical changes to the sediment from burrowing, and increased transport of dissolved P from sediment porewater to surface waters. Management of ditches should consider effects of burrowing benthic invertebrates on physical and biogeochemical processes at the sediment–water interface. Habitat manipulation in ditches could lead to unpredicted changes in nutrient dynamics mediated by changes to the burrowing benthic invertebrate community.

Microtopographic differences in soil properties and microbial community composition at the field scale

Soil biogeochemical processes, such as soil-atmosphere greenhouse gas (GHG) fluxes, are highly variable in space and time, even in homogenously managed agricultural fields. Although soil properties which regulate biogeochemical processes are known to vary predictably with topographic position at the hillslope to watershed scale in landscapes with noticeable topographic relief, the role of microtopography in influencing field scale spatial variability in soil properties in relatively flat landscapes, such as the Midwest U.S., is poorly understood. Moreover, microtopographic effects on soil microbial community composition and its functional importance in mediating biogeochemical processes have previously received little attention. We, therefore, investigated the effect of microtopography on soil properties and microbial community composition which may regulate soil biogeochemical process rates. We sampled paired poorly-drained depressional and well-drained upslope areas in eight replicate maize fields in Champaign County, Illinois. We observed microtopographic differences in soil pH, HCl-extractable Fe(III) concentrations, and soil organic C concentrations (P < 0.05). After normalizing for site variance, we also observed microtopographic differences in microbial community composition based on bacterial 16S rRNA, fungal ITS2, and nitrogen-cycling functional genes (archaeal and bacterial amoA, nirK, nirS, and Clade I nosZ), with soil pH and HCl-extractable Fe(III) exhibiting strong correlations with community structure (P < 0.05). Taxa capable of anaerobic metabolisms, such as Geobacter and Anaeromyxobacter, were relatively more abundant in poorly-drained, depressional soils (P < 0.05), suggesting a possible functional difference in the distinct communities. Overall, our findings demonstrate microtopographic differences in soil physicochemical properties and microbial community composition which could contribute to spatial variation in biogeochemical process rates.

Microfluidic qPCR Enables High Throughput Quantification of Microbial Functional Genes but Requires Strict Curation of Primers

Quantification of microbial functional genes enhances predictions of soil biogeochemical process rates, but reliance on low-throughput quantitative PCR (qPCR) limits the scope of ecological studies to a handful of targets. Here, we explore whether microfluidic qPCR (MFQPCR) is a viable high-throughput alternative for functional gene quantification, by evaluating the efficiency, specificity and sensitivity of 29 established and 12 newly designed primer pairs targeting taxonomic, nitrogen-cycling and hydrocarbon degradation genes in gDNA soil extracts, under three different sets of MFQPCR assay conditions. Without curation, commonly-used qPCR primer pairs yielded an extreme range of reaction efficiencies (25.9% - 100.1%), but when conditions were optimized, MFQPCR produced copy-number estimates comparable to traditional qPCR. To guide microbial soil ecologists considering adoption of MFQPCR, we present suggestions for primer selection, including omission of inosines, degeneracy scores of < 9, amplicon sizes of ≤ 211 bp, and GC content of 32-61%. We conclude that, while the nanoliter reaction volumes, rapid thermocycling and one-size-fits-all reaction conditions of MFQPCR necessitates more stringent primer selection criteria than is commonly applied in soil microbial ecology, the ability to quantify up to 96 targets in 96 samples makes MFQPCR a valuable tool for monitoring shifts in functional community abundances. MFQPCR will particularly suit studies targeting multiple clade-specific functional genes, or when primer design is informed by previous knowledge of the environment.

Sharp water column stratification with an extremely dense microbial population in a small meromictic lake, Trekhtzvetnoe.

Located on the shore of Kandalaksha Bay (the White Sea, Russia) and previously separated from it, Trekhtzvetnoe Lake (average depth 3.5 m) is one of the shallowest meromictic lakes known. Despite its shallowness, it features completely developed water column stratification with high-density microbial chemocline community (bacterial plate) and high rates of major biogeochemical processes. A sharp halocline stabilizes the stratification. Chlorobium phaeovibrioides dominated the bacterial plate, which reached a density of 2 × 108 cell ml-1 and almost completely intercepts H2 S diffusion from the anoxic monimolimnion. The resulting anoxygenic photosynthesis rate reached 240 μmol C l-1 day-1 , exceeding the oxygenic photosynthesis rate in the mixolimnion. The rates of other processes are also high, reaching 4.5 μmol CH4 l-1 day-1 for methane oxidation and 35 μmol S l-1 day-1 for sulfate reduction. Metagenomic analysis demonstrated that the Chl. phaeovibrioides population in the bacterial plate layer had nearly clonal homogeneity, although some fraction of these cells harbour a plasmid. The Chlorobium population was associated with bacteriophages that share homology with CRISPR spacers in the host. These features make the ecosystem of the Trekhtzvetnoe Lake a valuable model for studying regulation and evolution processes in natural high-density microbial systems.

Coupling biogeochemical process rates and metagenomic blueprints of coastal bacterial assemblages in the context of environmental change

Bacteria are major drivers of biogeochemical nutrient cycles and energy fluxes in marine environments, yet how bacterial communities respond to environmental change is not well known. Metagenomes allow examination of genetic responses of the entire microbial community to environmental change. However, it is challenging to link metagenomes directly to biogeochemical process rates. Here, we investigate metagenomic responses in natural bacterioplankton communities to simulated environmental stressors in the Baltic Sea, including increased river water input, increased nutrient concentration, and reduced oxygen level. This allowed us to identify informative prokaryotic gene markers, responding to environmental perturbation. Our results demonstrate that metagenomic and metabolic changes in bacterial communities in response to environmental stressors are influenced both by the initial community composition and by the biogeochemical factors shaping the functional response. Furthermore, the different sources of dissolved organic matter (DOM) had the largest impact on metagenomic blueprint. Most prominently, changes in DOM loads influenced specific transporter types reflecting the substrate availability and DOC assimilation and consumption pathways. The results provide new knowledge for developing models of ecosystem structure and biogeochemical cycling in future climate change scenarios and advance our exploration of the potential use of marine microorganisms as markers for environmental conditions.

Bilayer Infiltration System Combines Benefits from Both Coarse and Fine Sands Promoting Nutrient Accumulation in Sediments and Increasing Removal Rates.

Infiltration systems are treatment technologies based on water percolation through porous media where biogeochemical processes take place. Grain size distribution (GSD) acts as a driver of these processes and their rates and influences nutrient accumulation in sediments. Coarse sands inhibit anaerobic reactions such as denitrification and could constrain nutrient accumulation in sediments due to smaller specific surface area. Alternatively, fine sands provide higher nutrient accumulation but need a larger area available to treat the same volume of water; furthermore, they are more susceptible to bioclogging. Combining both sand sizes in a bilayer system would allow infiltrating a greater volume of water and the occurrence of aerobic/anaerobic processes. We studied the performance of a bilayer coarse-fine system compared to a monolayer fine one-by triplicate-in an outdoor infiltration experiment to close the C-N-P cycles simultaneously in terms of mass balances. Our results confirm that the bilayer coarse-fine GSD promotes nutrient removal by physical adsorption and biological assimilation in sediments, and further it enhances biogeochemical process rates (2-fold higher than the monolayer system). Overall, the bilayer coarse-fine system allows treating a larger volume of water per surface unit achieving similar removal efficiencies as the fine system.

Seasonal Dynamics of Biogeochemical Processes in the Water Column of the Northeastern Black Sea

Integrated studies on the hydrochemistry and water column rates of microbial processes in the eastern sector of the Black Sea along a standard 100-miles transect off Gelendzhik from the coast to the central part of the sea at water depths of 100–2170 m show that a series of warm winters and the absence of intense convective winter mixing resulted in a relatively low content of suspended particulate matter (SPM), particulate organic carbon (POC), and nutrients in the water column in March 2009. The relatively high SPM concentrations and the presence of isotopically light POC at the offshore station are indicative of the supply of terrigenous material from land and low contributions of phytoplanktonic organic matter to the composition of SPM. This may explain the low rates of biogeochemical processes in the water column near the coast. The surface layer at deep-water stations is dominated by isotopically heavy phytoplanktonic organic matter. This suggests that the supply of terrigenous material from land was insufficient in offshore deep-water areas. Therefore, warm winters and insufficient nutrient supply do not prevent photosynthesis in the photic layer of the deep-water zone, which generates organic substrates for heterotrophic aquatic communities. The results of isotopic analysis of POC, measurements of the rates biogeochemical processes, and the hydrochemical characteristics of the water column can be used to determine the nature and seasonal variability of the POC composition.