Shortening Rate Research Articles

Telomere- and oxidative stress dynamics in Psittacidae species with different longevity trajectories.

Telomeres, conserved DNA sequences at chromosome ends, naturally shorten with age, exacerbated by external factors like environmental challenges and reproduction. Birds, particularly psittacine, are gaining prominence as new aging models over the years because of their unique characteristics. This study explores erythrocyte telomere length (TL) and oxidative stress markers in plasma of long- and short-lived captive birds of the order Psittaciformes over four years. Long-lived birds consistently exhibited longer TL than short-lived ones (p = 0.012) but experienced a more pronounced TL shortening rate (p < 0.001) than short-lived ones. Breeding individuals experienced increased TL shortening compared to non-reproductive counterparts in long-lived birds (p = 0.008). Interestingly, long-lived birds showed a higher total antioxidant capacity than short-lived ones (p < 0.001), which was also increased during breeding (p = 0.026). A significant correlation was found between the telomere length shortening rate within the 4years of study and the accumulated oxidative stress (r = 0.426, p = 0.069) in short-lived birds. These findings shed light on TL and oxidative stress dynamics over time, revealing distinct patterns influenced by life-traits among longevity groups.

High-intensity interval training improves cardiomyocyte contractile function and myofilament sensitivity to intracellular Ca2+ in obese rats.

High-intensity interval training (HIIT) has shown significant results in addressing adiposity and risk factors associated with obesity. However, there are no studies that investigate the effects of HIIT on contractility and intracellular Ca2+ handling. The purpose of this study was to explore the impact of HIIT on cardiomyocyte contractile function and intracellular Ca2+ handling in rats in which obesity was induced by a saturated high-fat diet (HFD). Male Wistar rats were initially randomized into a standard diet and a HFD group. The experimental protocol spanned 23weeks, comprising the induction and maintenance of obesity (15weeks) followed by HIIT treatment (8weeks). Performance was assessed using the maximum oxygen consumption test ( ). Evaluation encompassed cardiac, adipose and skeletal muscle histology, as well as contractility and intracellular Ca2+ handling. HIIT resulted in a reduction in visceral area, an increase in , and an augmentation of gastrocnemius fibre diameter in obese subjects. Additionally, HIIT led to a decrease in collagen fraction, an increase in percentage shortening, and a reduction in systolic Ca2+/percentage shortening and systolic Ca2+/maximum shortening rates. HIIT induces physiological cardiac remodelling, enhancing the contractile function of cardiomyocytes and improving myofilament sensitivity to Ca2+ in the context of obesity. This approach not only enhances cardiorespiratory and physical performance but also reduces visceral area and prevents interstitial fibrosis.

TMT-based proteomics reveals methylprotodioscin alleviates oxidative stress and inflammation via COX6C in myocardial infraction

The effect of methylprotodioscin (MPD), a steroidal saponin obtained from medicinal plants, on myocardial infarction (MI) remains elusive. In this study, HL-1 and AC16 cells were subjected to injury induced by hypoxic environment, and a mouse model of MI was established by ligating the left anterior descending. MPD significantly increased viabilities and proliferations, improved the stability of MMP, reduced ROS and inflammatory factor levels in hypoxia cardiomyocytes. Moreover, MPD significantly improved cardiac functions, increased the ventricular ejection fraction and short axis shortening rate of mice with MI, reduced the infarction area, alleviated oxidative stress and increased ATPase activities. Then, differentially expressed proteins (DEPs) were discovered and evaluated using tandem mass tag (TMT)-based proteomics and bioinformatics approaches. Compared with sham group, there were 420 DEPs in the cardiac tissue of MI group, likewise, 163 DEPs in MPD group were identified compared to MI group. By validating, the expression of COX6C was elevated in MI group and declined in MPD groups, consistent with the TMT-based proteomics results. Correspondingly, p-NF-κB expression was downregulated, while Nrf2 and SOD expressions were upregulated by MPD. Moreover, si-COX6C transfection blocked the regulatory effects of MPD on COX6C-mediated inflammation and oxidative stress in MI. Our findings indicate that MPD, a naturally occurring active ingredient, could effectively improve cardiac function. Its ability may result from regulating COX6C to reduce oxidative stress and suppress inflammation, suggesting that MPD is very attractive for the treatment of MI.

Maastrichtian-Cenozoic erosional history of the northern Peruvian Amazonian Andes implications for the Eastern Cordillera evolution (Huallaga Basin, northern Peru)

Late Cretaceous-Cenozoic Andean mountain building may have experienced various phases of orogenesis in response to variations the dip angle of the subducting slab, distinct magmatic pulses, and shortening rates of the orogen. The timing of the Eastern Cordillera (EC) erosion and the relationship between the Andean orogenesis and the establishing of the transcontinental Amazon River remains unclarified. The erosional history of the Amazon Andes and the timing of these orogenic events may be revealed by the late Cretaceous-Cenozoic sedimentary record of the Amazonian retroarc foreland basin. We investigate the provenance of the Maastrichtian-Cenozoic deposits of the Huallaga Basin based on major and trace elements concentrations, SmNd isotopic composition, and UPb zircon dating. We also refined the Oligocene paleoenvironment and calculated Paleogene sedimentation rates. Our results show that despite the 25 Myr-long sedimentary hiatus, both the Maastrichtian and Eocene units show dominant sourcing from the magmatic arc of the Western Cordillera (WC). Increases in Paleozoic and Famatinian zircon grains and a shift toward more negative ƐNd(0) values, indicate dominant EC sources during the Oligocene to middle Miocene. This change in provenance area is Rupelian in age based on the Maximum Likelihood Age (MLA) of JUA22 (29.70 ± 0.62 Ma), suggesting that the onset of Peruvian EC erosion started at ∼30 Ma. The erosion of the EC was accompanied by an increase in sedimentation rates and the development of a meandering fluvial system. Finally, a substantial decrease in zircon grains derived from the Cretaceous and Cenozoic Andean arcs from late Miocene to Recent sediments suggests no contribution of the WC. In contrast, an increase in Paleozoic magmatic arc zircon grains indicates sources in the EC. We compare our findings to constrain the erosional history of the Amazonian Andes and investigate the relationship between the timing of EC uplift and the onset of the transcontinental Amazon drainage system during the Cenozoic.

Quaternary Deformation along the Gobi–Tian Shan Fault in the Easternmost Tian Shan (Harlik Mountain), Central Asia

The Tian Shan is a typical active intracontinental orogenic belt that is driven by the ongoing indentation of India into Eurasia. However, the geological features of Quaternary deformation, especially in the easternmost sector near Harlik Mountain, remain elusive. Field observations, topographic analysis, and Electron Spin Resonance (ESR) dating were employed to comprehensively assess the deformation features and evaluate the deformation pattern for this region during the Quaternary period. The results disclose evidence of deformation in the northern and southern foreland basins of Harlik Mountain. In the Barkol Basin to the north, crustal shortening results in the formation of surface scarps and folds, indicating north-directed thrusting, with a shortening rate of ~0.15 mm/yr. In the Hami Basin, the north-directed thrust elevates the granites, which offset the alluvial fans, with a shortening rate of ~0.18 mm/yr. Together with the shortening along the boundary fault, the aggregated north–south shortening rate is approximately 0.69 mm/yr in the easternmost Tian Shan, corresponding with the differential motion rate between the north and south Harlik Mountain revealed by the GPS velocity. These findings imply that, distal to the collision zone, tectonic strain in the eastern Tian Shan is primarily accommodated through the reactivation of pre-existing strike–slip faults, with crustal shortening concentrated at the overlapping position of parallel northeast-trending left-lateral strike–slip faults.

An effective strategy for treatment of severe kyphosis secondary to ankylosing spondylitis: one-level modified osteotomy combined with shoulders lifting correction method

BackgroundSevere kyphosis is a common condition in patients with advanced ankylosing spondylitis (AS). Although two-level osteotomy may serve as a potential alternative, it is often associated with increased blood loss and elevated surgical risks. To date, the optimal treatment for the challenging condition remains unclear. This study aims to introduce an effective strategy for the treatment of severe kyphosis secondary to AS, using one-level modified osteotomy combined with shoulders lifting correction method.MethodsSeventy AS kyphosis who were treated with the strategy from 2012 to 2022, were reviewed retrospectively. All patients were followed up for a minimum duration of 2 years. Spinal and pelvic parameters were measured, including pelvic tilt (PT), pelvic incidence (PI), sacral slope (SS), lumber lordosis (LL), PI and LL mismatch (PI-LL), thoracic kyphosis, global kyphosis (GK), T1 pelvic angle (TPA), sagittal vertical axis (SVA), osteotomized vertebral angle (OVA), and chin-brow vertical angle (CBVA). Parameters of local osteotomized complex were measured and calculated, including the height of osteotomized complex and the length of spinal cord shortening. Clinical outcome was evaluated using Scoliosis Research Society-22 and Oswestry Disability Index scores.ResultsSeventy patients with average age of 39.8 years were followed-up for 29.3 months. Average operation time was 373.5 min, and average blood loss was 751.0 ml. Postoperatively, sagittal balance was successfully restored. GK decreased from 90.6° to 35.6°, LL decreased from 8.0° to -35.1°, TPA decreased from 56.8° to 27.8°, and SVA decreased from 24.4 cm to 8.7 cm (P < 0.05). A harmonious and matched spinopelvic alignment was achieved. PT decreased from 37.2° to 26.3°, PI-LL decreased from 54.1° to 10.2°, and SS increased from 9.2° to 19.7°(P < 0.05). Horizontal vision was obtained with postoperative CBVA of 8.8°. Average OVA correction was up to 47.3°, and the spinal cord was shortened by 24.3 mm, with a shortening rate of 36.0%. All patients demonstrated a favorable clinical outcome. No permanent nerve damage, screw loosening, rod breakage and main vascular injury were observed. One case required revision surgery due to screw cap loosening and delayed union. Solid bone fusion was achieved in all other patients.ConclusionsOne-level modified osteotomy combined with shoulders lifting correction method is a safe and effective strategy for the treatment of severe AS kyphosis. This strategy offers a promising alternative for managing severe AS kyphosis, and may be particularly well-suited for individuals with concurrent osteoporosis.Level of evidenceLevel IV, therapeutic study.

21Ne and 10Be dating of folded fluvial terraces: Constraining active deformation of the Guman fold along the foothill of the western Kunlun mountains (Xinjiang, China)

Characterizing the active deformation of the foreland is particularly valuable for understanding the dynamics of regional structural evolution within active contractional orogens. We focus on the Guman fold, one of the most prominent fold-and-thrust belts along the foothill of the Western Kunlun Mountains (Xinjiang, China). The anticline growing above the blind thrust faults progressively deforms river terraces. However, contemporaneous terrace surfaces are buried under young deposits north of the fold. We propose a novel method that extends terrace surfaces above the forelimb of the fold within the extent of deformation determined on the seismic reflection profile, to estimate the sediment thickness after abandonment of the terraces. Furthermore, cosmogenic nuclide (10Be and 21Ne) depth profile dating was used to determine the exposure ages of the two terrace surfaces: 413–673 kyr for T1b and 3.7–5.2 Myr for T3b. Combining the age and deformation amount, the slip rate on the frontal fault ramp within the fold is estimated to be 0.4+0.2/-0.1 mm/yr and 0.17+0.07/-0.03 mm/yr since the abandonment of the T1b and the T3b terrace respectively. These rates represent the recent crustal shortening rate across the Guman fold and likely account for most of the total crustal shortening rate across the Western Kunlun Mountains. However, these rates appear to be notably lower by an order of magnitude compared to the long-term (∼23 Ma) average crustal shortening rate (∼1.1–2.6 mm/yr) in this region. This may indicate a rapid slowing down of the deformation at the scale of the whole Western Kunlun Mountains, possibly related to a regional reorganization.

Shortening of telomere length may be associated with inflammatory cytokine levels in patients with bipolar disorder

BackgroundBipolar disorder (BD) is hypothesized to be associated with accelerated biological aging. Telomere length (TL) is a biomarker of aging, and although TL decreases with each cell division, the rate of telomere shortening may be affected by inflammation. We aimed to investigate whether TL is decreased in BD patients and to determine the association between TL and inflammatory markers in such patients. Methods137 BD patients and 118 healthy controls (HCs) were recruited. Leukocyte TL and plasma levels of cytokines [tumor necrosis factor (TNF)-α, interleukin (IL)-8, IL-6, IL-10, transforming growth factor (TGF)-β1], C-reactive protein (CRP), and brain-derived neurotrophic factor (BDNF) were assessed. ResultsTL did not differ significantly between the BD patients and HCs after adjustment for potential confounding factors (P = 0.79). TL was significantly negatively associated with age (β = −0.007, P < 0.001). In addition, log TNF-α levels were significantly negatively associated with TL (P = 0.009), in both the BD patients (P = 0.02) and HCs (P = 0.05). ConclusionWe found a significant association between TNF-α levels and TL shortening in both BD patients and HCs. However, BD patients did not display increased TL shortening relative to HCs. Studies that involve larger sample sizes and control for the heterogeneity of BD participants will be needed.

A multi-strategy improved sparrow search algorithm for mobile robots path planning

Abstract Path planning for mobile robots plays a vital role in task execution, given the constraints imposed by environments and energy resources. It poses a significant challenge for mobile robots, requiring them to find a feasible path between the start point and target point that is obstacle-free and as short as possible. To address the challenge of path planning, a multi-strategy improved sparrow search algorithm with crazy operator (CMISSA) is proposed. Firstly, Tent chaos mapping and reverse learning are introduced into the population initialization of sparrow search algorithm (SSA) to enhance the uniformity and effectiveness of the initial population distribution. Secondly, adaptive parameters are applied in SSA to maintain a balance between exploitation and exploration. Thirdly, to prevent SSA from getting trapped in local optima, the crazy operator is used to perturb the population position. Finally, a novel adaptive boundary control strategy is introduced to handle the location of individuals that have crossed the boundary. In addition, the experimental results on 15 classical benchmark functions show that CMISSA has better optimization performance than other 10 comparison algorithms. Furthermore, in the path planning experimental results, the results of comparing CMISSA with 5 comparison algorithms on 5 different environments reveal CMISSA's average path shortening rates were 34.90%, 20.11%, 29.01%, 51.97%, 37.42%, respectively. It is further demonstrated that CMISSA has superior availability for solving mobile robots path planning.

Association between osteoporosis and the rate of telomere shortening.

A shorter leukocyte telomere length (LTL) is reported to be associated with age-related diseases, including osteoporosis. Many studies have tried identifying the association between LTL and osteoporosis, although it remains controversial. This study aimed to determine whether osteoporosis is independently associated with LTL shortening in a prospective longitudinal cohort. The KBASE study is an independent multicenter prospective cohort in South Korea, which began in 2014. We compared the LTL values for each participant at baseline and over a 2-year follow-up period. Boxplots were used to demonstrate the differences in the change in LTL over a 2-year follow-up according to osteoporosis. Multivariable linear regression was conducted to identify whether osteoporosis is independently associated with the rate of telomere shortening. A total of 233 subjects (from 55 to 88 years) from the KBASE cohort were finally enrolled in the study. We observed that the LTL decreased by approximately 1.2 kbp over 2 years. While the LTL decreased as age increased, the rate of LTL shortening did not increase with age. Multivariable linear regression analysis indicated that only osteoporosis was independently associated with rapid LTL shortening over 2 years (B, -8.08; p = 0.038). We sought to identify an association between osteoporosis and LTL shortening in an independent prospective cohort. We found that participants with osteoporosis had significantly faster LTL shortening over 2 years than those without osteoporosis. We hope this study will help elucidate the underlying mechanisms in the relationship between LTL and osteoporosis in the future.

Optimizing the compression resistance of low-nickel stainless steel coronary stents using finite element and response surface methodology

Considering the high strength and excellent biocompatibility of low-nickel stainless steel, this paper focused on optimizing the design of a vascular stent made from this material using finite element analysis (FEA) combined with the response surface methodology (RSM). The aim is to achieve the desired compressive resistance for the stent while maintaining a thin stent wall thickness. The parameters of the stent’s support unit width (H), strut width (W), and thickness (T) were selected as input parameters, while the output parameters obtained from FEA included the compressive load, the equivalent plastic strain (PEEQ), axial shortening rate, radial recoil rate, and metal coverage rate. The mathematical models of input parameters and output parameters were established by using the Box Behnken design (BBD) of RSM. The model equations were solved under constrained conditions, and the optimal structural parameters, namely H, W, and T, were finally determined as 0.770 mm, 0.100 mm, and 0.075 mm respectively. In this situation, the compression load of the stent reached the target value of 0.38 N/mm; the PEEQ resulting from the stent expansion was small; the axial shortening, radial recoil, and metal coverage index were all minimized within the required range.

Active shortening and aseismic slip along the Cephalonia Plate Boundary (Paliki Peninsula, Greece): Evidence from InSAR and GNSS data

We present a comprehensive analysis of geodetic data, including InSAR and GNSS, to assess the interseismic deformation of the Paliki peninsula in western Cephalonia, Greece. The region is prone to frequent earthquakes, due to its proximity to the Cephalonia Transform Fault (CTF), a 140 km long dextral strike-slip fault (striking NNE-SSW) that accommodates the relative motion between the Apulian and Aegean lithospheric plates. Our analysis covers the period from 2016 to 2022 and leverages LiCSBAS, an open-source package, for InSAR time series analysis with the N-SBAS method. The results of the InSAR analysis demonstrate deformation rates between 2 and 5 mm/yr in the line-of-sight (LOS) direction of the satellite. We constructed E-W velocity profiles that provided a velocity gradient terminating against the outcropping trace of the seismic fault of the 3 February 2014 earthquake (M6.1) near Atheras. This geodetic evidence indicates a ∼ 1 mm/yr minimum aseismic slip (fault creep) motion along the February 2014 seismic fault, during 2016–2022. Positive LOS values in both satellite imaging geometries show that the coastal town of Lixouri experiences uplift of 1 mm/yr. East-West velocity cross-sections across western Cephalonia including Gulf of Argostoli reveal several velocity discontinuities, possibly bounded by active faults and/or landslides. The E-W shortening rate between Lixouri and Argostoli areas amounts to 1.5 mm/yr corresponding to −187 ns/yr (nanostrain/yr) of tectonic strain. Our results suggest a complex deformation pattern on the Paliki peninsula with strain accumulation along strike-slip and reverse-slip faults. We also inverted GNSS velocities from Italy and Greece, across the Cephalonia segment of CTF (assuming elastic half-space) and obtained a locking depth of 13 km, and a slip rate of 17.3 ± 0.8 mm/yr.

Effect of moxibustion at "Xinshu" (BL15) and "Feishu" (BL13) on expression of TRPV1 and CGRP in the myocardial tissue of chronic heart failure rats.

To observe the effect of moxibustion at "Xinshu" (BL15) and "Feishu" (BL13) on transient receptor potential vanilloid type 1(TRPV1), calcitonin gene-related peptide (CGRP), and serum interleukin-10 (IL-10) in the myocardial tissue of rats with chronic heart failure (CHF), so as to explore its underlying mechanisms in improvement of CHF. Male SD rats were randomly divided into the normal, model, moxibustion, capsaicin, moxibustion + capsaicin, and moxibustion + solvent groups, with 10 rats in each group. The CHF model was established by permanent ligation of the anterior descending branch of the left coronary artery. Mild moxibustion was applied to bilateral BL13 and BL15 for 30 min once daily for 4 weeks. Rats in the capsaicin group were smeared with capsaicin in the acupoint area once a day for 4 weeks. For rats of the moxibustion + capsaicin and moxibustion + solvent groups, capsaicin and solvent were applied to the acupoint area before moxibustion for 4 weeks, respectively. The ejection fraction (EF) and left ventricular fractional shortening rate (FS) were examined by echocardiography. HE staining was used to observe the myecardial morphological structure. The mRNA and protein expression levels of TRPV1, CGRP and galectin-3 (Gal-3) in myocardial tissue were detected by real-time quantitative PCR and Western blot, respectively. The content of IL-10 in serum was detected by ELISA. After modeling, the pathological changes of myocardium (as cardiac muscle fiber disorder, inflammatory cell infiltration, etc.) were obvious, and the EF, FS, serum IL-10, protein and mRNA exspression of TRPV1 and CGRP were significantly decreased (P<0.01) in the model group compared with the normal group, while the protein and mRNA exspression of Gal-3 were significantly up-regulated (P<0.01). Following the interventions, the above-mentioned indexes were all reversed in moxibustion, capsaicin, and moxibustion + capsaicin groups (P<0.01), and the effect of moxibustion + capsaicin was the best (P<0.05, P<0.01). Moxibustion can reduce myocardial injury and improve cardiac function in CHF rats, which may be related to its effects in up-regulating the expression of TRPV1 and CGRP, and down-regulating the expression of Gal-3 to alleviate myocardial fibrosis.

Exosomes derived from cancer cells relieve inflammatory bowel disease in mice

Exosome therapy has garnered significant attention due to its natural delivery capabilities, low toxicity, high biocompatibility, and potential for personalised treatment through engineering modifications. Recent studies have highlighted the ability of tumour cell-derived exosomes (TDEs) to interact with immune cells or modify the immune microenvironment to suppress host immune responses, as well as their unique homing ability to parental cells. The core question of this study is whether this immunomodulatory property of TDEs can be utilised for the immunotherapy of inflammatory diseases. In our experiments, we prepared exosomes derived from murine colon cancer cells CT26 (CT26 exo) using ultracentrifugation, characterised them, and conducted proteomic analysis. The therapeutic potential of CT26 exo was evaluated in our dextran sulphate sodium salt (DSS)-induced inflammatory bowel disease (IBD) mouse model. Compared to the control and 293 T exo treatment groups, mice treated with CT26 exo showed a reduction in the disease activity index (DAI) and colon shortening rate, with no noticeable weight loss. Haematoxylin and eosin (H&E) staining of colon paraffin sections revealed reduced inflammatory infiltration and increased epithelial goblet cells in the colons of CT26 exo-treated group. Furthermore, we conducted preliminary mechanistic explorations by examining the phenotyping and function of CD4+ T cells and dendritic cells (DCs) in the colonic lamina propria of mice. The results indicated that the ameliorative effect of CT26 exosomes might be due to their inhibition of pro-inflammatory cytokine secretion by colonic DCs and selective suppression of Th17 cell differentiation in the colon. Additionally, CT26 exo exhibited good biosafety. Our findings propose a novel exosome-based therapeutic approach for IBD and suggest the potential application of TDEs in the treatment of inflammatory diseases.

Late Pleistocene Fault Slip Rate within the Bole Basin: Insights into deformation kinematics in the Central Tian Shan

Tian Shan is a vast and highly seismically active intracontinental mountain range. GPS measurements and field studies have shown that E-W and NW-SE trending thrusts and fault-related folds are distributed across central Tian Shan. However, few studies have determined the fault and fault-related-fold activity in the Bole Basin, the northernmost part of central Tian Shan. In this study, we focus on the Alashak Fold, which is situated along the southern margin of the Bole Basin. Using high-resolution uncrewed aerial vehicle-Digital Elevation Model data, field observations, and detailed mapping, we determined that the Alashak Fold is characterized by a trishear fault-propagation fold. Along the Alashak River, we identified five levels of fluvial surfaces, the T3 terrace was continuously preserved across the fold. Radiocarbon and surficial 10Be dating were used to determine the exposure age of the deformed T3 terrace. The folding characteristics of T3, trishear inversed modeling, and abandonment age indicate that the Alashak Fault has an uplift rate of 0.8 ± 0.3 mm/yr, a dip-slip rate of 4.2 ± 1.2 mm/yr, and a crustal shortening rate of 4.0 ± 1.2 mm/yr since the late Pleistocene, respectively. The Alashak and Latgan Faults intersect at a depth of 1.2 km, enclosing a wedge-shaped block. Our study emphasizes that tectonic activity along the southern margin of the Bole Basin has played a significant role in north-south shortening within central Tian Shan, as measured by geological measurements since the late Pleistocene. The Alashak Fault can potentially generate moderate–strong (Mw 5.0–7.0) earthquakes, while the Alashak and Latgan Faults can generate major (Mw 7.2) earthquakes.

Ultrasound-based visualization measurement of hyoid-mandibular motion for assessing the efficacy of acupuncture in treating post-stroke dysphagia: A clinical study

ObjectiveThe objective of this study was to utilize ultrasonography to measure the movement-related characteristics of the hyoid bone-mandible in patients with dysphagia in order to quantitatively evaluate the clinical efficacy of acupuncture as a treatment for dysphagia following brain infarction. MethodsA total of 63 patients with dysphagia following a brain infarction were randomly assigned into two groups: one receiving meridian point treatments and the other receiving non-meridian and non-point treatments. The meridian point group received routine treatment using the meridian point acupuncture method, and the true acupuncture intervention mode of qi arrival plus swift pricking blood therapy was utilized. The non-meridian and non-acupoint group received conventional treatment using the pseudo-acupuncture mode of non-acupoint without qi arrival. Both groups received treatment once daily, five times per week, for a duration of four weeks. Video fluoroscopic swallowing study (VFSS) was used to diagnose dysphagia in all patients, and FOIS and PAS were also used for clinical assessment. Results(i) Clinical efficacy comparison: After 4 weeks of treatment, the meridian group showed superior efficacy compared to the non-meridian and non-acupuncture group (P < 0.05). The FOIS score of the meridian acupoint group was also higher than that of the non-meridian and non-acupoint group (P < 0.05). In addition, the dilute fluid food PAS score in the meridian acupoint group was lower than that in the non-meridian and non-acupoint group (P < 0.05). (ii) Comparison of ultrasonography: The hyoid-mandibular comparison of the shortening distance, shortening rate, and movement speed of the bone movement were measured in both groups after 2 weeks and 4 weeks of treatment. The results indicated that the improvement in the meridian group was better than that in the non-meridian and non-acupoint group in all measurements (P < 0.05). ConclusionPatients with swallowing disorders exhibit impaired hyoid-mandibular movement. However, the implementation of true acupuncture intervention can enhance hyoid-mandibular movement function, ultimately leading to an improvement in swallowing function.

Effects of persistent organic pollutants on telomere dynamics are sex and age-specific in a wild long-lived bird

Chemical pollution is a major man-made environmental threat to ecosystems and natural animal populations. Of concern are persistent organic pollutants (POPs), which can persist in the environment for many years. While bioaccumulating throughout the lives of wild animals, POPs can affect their health, reproduction, and survival. However, measuring long-term effects of POPs in wild populations is challenging, and therefore appropriate biomarkers are required in wildlife ecotoxicology. One potential target is telomere length, since telomere preservation has been associated to survival and longevity, and stressors as chemical pollution can disrupt its maintenance. Here, we investigated the effects of different classes of POPs on relative telomere length (RTL) and its rate of change (TROC) in wild long-lived Alpine swifts (Tachymarptis melba). As both RTL and TROC are often reported to differ between sexes and with chronological age, we tested for sex- and age-specific (pre-senescent vs. senescent, ≥ 9 age of years, individuals) effects of POPs. Our results showed that senescent females presented longer RTL and elongated telomeres over time compared to pre-senescent females and males. These sex- and age-related differences in RTL and TROC were influenced by POPs, but differently depending on whether they were organochlorine pesticides (OCPs) or industrial polychlorinated biphenyls (PCBs). OCPs (particularly drins) were negatively associated with RTL, with the strongest negative effects being found in senescent females. Conversely, PCBs led to slower rates of telomere shortening, especially in females. Our study indicates diametrically opposed effects of OCPs on RTL and PCBs on TROC, and these effects were more pronounced in females and senescent individuals. The mechanisms behind these effects (e.g., increased oxidative stress by OCPs; upregulation of telomerase activity by PCBs) remain unknown. Our results highlight the importance in wildlife ecotoxicology to account for sex- and age-related effects when investigating the health effects of pollutants on biomarkers such as telomeres.

Short term follow-up of femoral neck dynamic cross screw system and threaded cannulated screw in the treatment of vertically unstable femoral neck fractures

To analyze and compare the clinical effects of femoral neck dynamic cross screw system (FNS) and cannulated screws(CS) in the treatment of vertically unstable femoral neck fractures. The clinical data and short-term follow-up results of 40 patients with vertically unstable femoral neck fractures admitted from July 2020 to August 2021 were retrospectively analyzed. According to different internal fixation methods, 40 patients were divided into two groups, 20 cases in FNS group included 11 males and 9 females with a median of 58.5(50.3, 62.5) years old, and 20 in CS group included 9 males and 11 females with a median of 52.0(40.5, 58.0) years old. The operation time, knife edge length, blood loss and treatment cost of two gruops were observed and compared. The postoperative fracture healing and internal fixation were evaluated with X-ray imaging data, and the femoral neck shortening of the affected side was measured. The incidence of thigh irritation, the time of partial weight bearing and full weight bearing, early necrosis of femoral head, reoperation revision and Harris scores were compared between two groups. FNS group was followed up for 18.0(15.0, 19.0) months, CS group for 17.0(15.0, 18.8) months. There was no significant difference in operation time, incision length and blood loss between two groups(P>0.05). The cost of diagnosis and treatment in FNS group was higher than that in CS group(P<0.001). In FNS group, there was no irritation sign of the affected side thigh, while in CS group, there were 6 cases with discomfort or irritation sign of the lateral thigh(P<0.05). The average time of partial weight bearing activity in CS group was later than that in FNS group(P<0.05); However, there was no significant difference in the activity time of complete weight bearing between two groups(P=0.011>0.05). At the last follow-up, the shortened length of the affected femoral neck in CS group was greater than that in FNS group(P<0.05). There was no early necrosis of femoral head and reoperation in both groups. There was no significant difference in Harris score between two groups 12 months after operation(P>0.05). FNS treatment of vertically unstable femoral neck fractures can significantly reduce the incidence of lateral thigh irritation sign, and effectively reduce the postoperative shortening rate of vertically unstable femoral neck fractures, which can provide a relatively stable anti rotation force and anti cutting force, so that patients can go to the ground relatively early, which is conducive to the recovery of the affected hip joint function after surgery. It is a new option for the surgical treatment of vertically unstable femoral neck fractures. However, due to the high cost of treatment, In clinical practice, appropriate surgical treatment is selected according to the actual situation.

Telomeres and the Rate of Living: Linking Biological Clocks of Senescence.

AbstractTwo prominent theories of aging, one based on telomere dynamics and the other on mass-specific energy flux, propose biological time clocks of senescence. The relationship between these two theories, and the biological clocks proposed by each, remains unclear. Here, we examine the relationships between telomere shortening rate, mass-specific metabolic rate, and lifespan among vertebrates (mammals, birds, fishes). Results show that telomere shortening rate increases linearly with mass-specific metabolic rate and decreases nonlinearly with increasing body mass in the same way as mass-specific metabolic rate. Results also show that both telomere shortening rate and mass-specific metabolic rate are similarly related to lifespan and that both strongly predict differences in lifespan, although the slopes of the relationships are less than linear. On average, then, telomeres shorten a fixed amount per unit of mass-specific energy flux. So the mitotic clock of telomere shortening and the energetics-based clock described by metabolic rate can be viewed as alternative measures of the same biological clock. These two processes may be linked, we speculate, through the process of cell division.

Discrete element modeling of distal deformation propagation in thrust wedge and implications for early deformation on northern Tibetan and Iranian Plateaus

Coulomb critical wedge theory predicts that thrust wedges would grow sequentially from the hinterland to the foreland, meaning that distal deformation occurs last. However, in the northern Tibetan and Iranian Plateaus, far away from the southern collision zones, widespread deformation occurs soon after collisions of Arabia and India with Eurasia. Additionally, despite the prevalence of weak lower crust and distal pre-existing faults or weak zones, their relationship to early distal deformation remains poorly understood. For this reason, we run systematic experiments of discrete element models involving basal décollement layer as well as distal pre-existing fault. Our model results reveal that (1) the presence of pre-existing faults is necessary for the occurrence of early distal deformation; (2) the early deformation of distal pre-existing fault is dependent on basal décollement strength and independent of model width; (3) strong basal décollement fails to activate the distal pre-existing faults, instead weak basal décollement can deform them at the early stage; (4) in the presence of weak basal décollement, a slower shortening rate not only facilitates greater shortening absorption by the distal pre-existing fault at the early stage but also results in a more pronounced deviation from sequentially-forward deformation propagation. These findings demonstrate that the preferential reactivation deformations of distal pre-existing faults are mechanically controlled by a weak basal décollement layer. Together with geological and geophysical observations, we suggest that the early deformations of northern Tibetan and Iranian Plateaus may be the result of the reactivation of pre-existing faults due to the existence of weak lower crust soon after collisions.