Ratchaburi Province Research Articles

Community learning for marketing management and digital marketing of Jasmine Rice by Nong Hin Housewives Group, Ratchaburi Province

Background and Aim: The Nong Hin Housewives Group has been instrumental in cultivating and marketing Jasmine rice, a product renowned for its quality. However, the group faces challenges in adopting modern marketing strategies, particularly in the digital realm. This study aims to analyze the current marketing practices, identify gaps in digital marketing skills, and develop a framework for effective community learning. By leveraging digital tools, the group can enhance its marketing efforts, reach a broader audience, and increase product sales. Materials and Methods: This study employs a mixed-methods approach, combining quantitative surveys and qualitative interviews. Surveys were distributed to 200 community members across different generations to assess their digital media usage and preferences. In-depth interviews were conducted with key members of the Nong Hin Housewives Group to understand their marketing challenges and aspirations. Data were analyzed using statistical methods for the survey responses and thematic analysis for qualitative data. Results: The findings reveal a significant disparity in digital media usage among different generations, with Facebook emerging as the most popular platform. The data indicate that 66.50% of respondents actively use Facebook for marketing purposes. The study also identified challenges related to digital literacy within the community, affecting the effective use of online marketing tools. Despite these challenges, there is a strong interest in learning and adapting to digital marketing strategies. Conclusion: The research underscores the importance of community learning in enhancing the marketing management and digital marketing capabilities of the Nong Hin Housewives Group. By investing in digital literacy training and developing tailored marketing strategies, the group can significantly improve its outreach and sales of Jasmine rice. The study emphasizes the need for ongoing support and resources to facilitate the transition into effective digital marketing practices.

A New Species of Aegus Macleay (Coleoptera: Lucanidae: Aegini) from western Thailand

In this study, Aegus crenulatus Pathomwattananurak & Jiaranaisakul, new species is described from Ratchaburi Province, western Thailand. Habitus and genitalia of both sexes are also illustrated and compared with the related species, Aegus sumatraensis Bomans, 1992 and Aegus taurus Boileau, 1899. The published records of Aegus linealis Didier, 1928 from Thailand are reidentified as A. taurus.

Association of Social Network with Physical Function Among Community-Dwelling Older Adults in Rural Thailand: A Cross-Sectional Study.

As the number of older adults in society increases, their social roles and networks, as well as their physical function, decrease. This study aimed to clarify the association between social networks and physical function among people aged ≥ 60 years in rural Thailand. This cross-sectional study was conducted in the Photharam District, Ratchaburi Province, Thailand. Participants were required to be at least 60 years old and be able to walk to the health center. Social networks were surveyed using the Thai version of Lubben Social Network Scores-6. Four physical function measures, namely hand grip strength, five-times-sit-to-stand test, timed up-and-go (TUG) test, and one-leg standing, were considered. Regression analysis was conducted with Lubben Social Network Scores-6 as the dependent variable and the four types of physical function as independent variables. A total of 497 older adults aged 60 years or more were enrolled; 82 were males, and 412 were females. The mean Lubben Social Network Scores-6 was 14.9 ± 5.7. Only the TUG test was associated in a single and multiple regression analysis with the Lubben Social Network Scores-6 as the dependent variable and the four physical function assessments as independent variables. The TUG test assessed the smoothness of normal standing and walking, which are essential physical functions for maintaining a social network and meeting people. This suggests a relationship between physical function and social network.

Confidence Intervals for the Mean and Difference of Means of Birnbaum-Saunders Distributions with Application to Wind Speed Data

This paper proposes the confidence intervals for the mean and difference of means of Birnbaum-Saunders (BirSau) distributions based on the Bootstrap confidence interval (BCI), Percentile bootstrap confidence interval (PBCI), Generalized confidence interval (GCI), Bayesian credible interval (BayCrI) and the highest posterior density (HPD). The simulation study used R statistical software to evaluate the coverage probabilities and average lengths. The concerning results of the mean suggest that HPD is the recommended method for constructing confidence intervals in the BirSau distributions, except for small sample sizes where the GCI method proves more efficient. For the difference of means, PBCI emerges as the preferred way to construct confidence intervals, except in some cases where small sample sizes with the HPD method are more efficient. Moreover, the average lengths of these proposed confidence intervals decreased as both sample size and shape parameters increased. To illustrate the effectiveness of the suggested confidence intervals, we applied them to wind speed datasets collected in Ayutthaya and Ratchaburi provinces, Thailand.

Two new species of Landouria Godwin-Austen, 1918 (Gastropoda, Camaenidae) from Thailand, with a key to Thai species.

The present work contains descriptions of two new species of Landouria and a key to Thai species of this genus. Landouriabella sp. nov. is described from isolated limestone hills in Ratchaburi and Phetchaburi provinces, western Thailand. This new species is characterized by its small depressed-globose shell without a peripheral keel, the presence of a small, thin lamella on the columellar side of the inner aperture, a long, cylindrical distally bent flagellum, a short penis with a rounded verge, and a short, thick free oviduct and vagina. The second new species, Landouriamonodon sp. nov. is described from sandstone hills in Kalasin Province, northeastern Thailand. This species has a depressed-conic shell with a blunt peripheral keel, a thick prominent lamella on the columellar side of the inner aperture, a short, finger-shaped, distally bent flagellum, and a long penis with a small, short verge.

Effect of Heat Stress on Subsequent Estrous Cycles Induced by PGF2α in Cross-Bred Holstein Dairy Cows.

This study aimed to determine the effect of heat stress (HS) on reproductive parameters (calving to first service (CTFS) and the first-service conception rate (FSCR)) and general physiological responses (rectal temperature (RT) and respiratory rate (RR)) in tropical cross-bred Holstein dairy cows raised in Ratchaburi province, Thailand. HS was determined using the temperature-humidity index (THI), calculated from temperature and humidity inside the barns, and was classified as moderate HS (THI: 80.67 ± 0.79) and mild HS (THI: 77.81 ± 1.09) in this study. Cows with detected corpus luteum were defined as cyclic cows and were injected with PGF2α at the beginning of the experimental period. Reproductive and physiological parameters were recorded. Cows showed significantly lower RT and RR in the mild HS group (38.47 ± 0.21 °C and 41.04 ± 4.55 bpm, respectively) than in the moderate HS group (38.87 ± 0.15 °C and 51.17 ± 10.52 bpm). The percentage of cows that ovulated after being induced by PGF2α and showed estrus signs was higher in the mild than the moderate HS groups (54.55% vs. 18.18%). Furthermore, the FSCR of cows under mild HS tended to be higher than that in the moderate HS group (42.11% and 15%, respectively) (p = 0.06), while the average CTFS interval was significantly shorter under mild HS than moderate HS (69.47 ± 18.18 and 84.60 ± 27.68 days, respectively). These results indicate that moderate HS impairs reproductive performance in crossbred Holstein cows, compared to mild HS conditions.

Identification of common human infectious and potentially zoonotic novel genotypes of Enterocytozoon bieneusi in cavernicolous bats in Thailand.

Enterocytozoon bieneusi is a common cause of human microsporidiosis and can infect a variety of animal hosts worldwide. In Thailand, previous studies have shown that this parasite is common in domestic animals. However, information on the prevalence and genotypes of this parasite in other synanthropic wildlife, including bats, remains limited. Several pathogens have been previously detected in bats, suggesting that bats may serve as a reservoir for this parasite. In this study, a total of 105 bat guano samples were collected from six different sites throughout Thailand. Of these, 16 from Chonburi (eastern), Ratchaburi (western), and Chiang Rai (northern) provinces tested positive for E. bieneusi, representing an overall prevalence of 15.2%. Based on ITS1 sequence analysis, 12 genotypes were identified, including two known genotypes (D and type IV) frequently detected in humans and ten novel potentially zoonotic genotypes (TBAT01-TBAT10), all belonging to zoonotic group 1. Lyle's flying fox (Pteropus lylei), commonly found in Southeast Asia, was identified as the host in one sample that was also positive for E. bieneusi. Network analysis of E. bieneusi sequences detected in this study and those previously reported in Thailand also revealed intraspecific divergence and recent population expansion, possibly due to adaptive evolution associated with host range expansion. Our data revealed, for the first time, multiple E. bieneusi genotypes of zoonotic significance circulating in Thai bats and demonstrated that bat guano fertilizer may be a vehicle for disease transmission.

Learning Management of Small-Scale Pig Farmers to Adopt Biogas Coproduction: A Case Study of Ratchaburi Province

Learning Management of Small-Scale Pig Farmers to Adopt Biogas Coproduction: A Case Study of Ratchaburi Province

Seasonal species composition, abundance and public health importance of mosquito vectors (Diptera: Culicidae) in Huai Tha Khoei Reservoir, Ratchaburi, Thailand

Abstract. Chaiphongpachara T, Laojuna S. 2024. Seasonal species composition, abundance and public health importance of mosquito vectors (Diptera: Culicidae) in Huai Tha Khoei Reservoir, Ratchaburi, Thailand. Biodiversitas 25: 1264-1275. Mosquitoes are vectors for pathogens that cause life-threatening diseases in humans and animals. Significant mosquito-borne diseases include malaria, dengue, chikungunya, Zika, Japanese encephalitis, and filariasis. Anthropogenic environmental changes can significantly impact the dynamics of disease vector populations, which can lead to outbreaks of mosquito-borne diseases. This study investigated the seasonal variation in species composition and abundance of mosquito vectors around the Huai Tha Khoei Reservoir in Ratchaburi Province, Thailand, across cool, hot, and rainy seasons. A total of 1363 mosquitoes, representing 30 species from five genera, were collected. In particular, 25 of these species, accounting for 83.33% of the total, were identified as vectors of human pathogens. The most dominant species across all seasons was Culex tritaeniorhynchus Giles 1901, representing 39.47% (n=538) of the total, followed by Anopheles barbirostris Wulp 1884 s.l. (14.38%, n = 196) and Culex vishnui Theobald 1901 (9.02%, n=123). The Shannon diversity index was highest during the rainy season (H’= 2.144) and lowest in the hot season (H’=1.788). This is consistent with the results of the UPGMA tree based on beta diversity, indicating that mosquito diversity in the rainy season differs significantly from that in the cool and hot seasons. The findings enhance our understanding of the impact of seasonality on mosquito vectors in this region, which is crucial for developing effective disease control strategies. Additionally, this study serves as a valuable reference for understanding the diversity of mosquito vectors in other areas.

A new species of krait of the genus Bungarus (Squamata, Elapidae) from Ratchaburi Province, western Thailand

A new species of krait of the genus Bungarus (Squamata, Elapidae) from Ratchaburi Province, western Thailand

The genus Zhengitettix Liang, 1994 (Orthoptera: Tetrigidae: Scelimeninae) from Thailand, with description of a new species.

Four species of the genus Zhengitettix Liang, 1994 from Thailand are listed. A new distribution of Z. albitarsus Storozhenko, 2013 is reported from Ratchaburi Province in the Tenasserim Range with a coloration description of live specimens. Z. binduseni sp. nov. is described from Ratchaburi Province, Thailand. This new species is easily distinguished from other member in this genus by the larger body size, the fastigium of vertex between eyes distinctly narrower toward end, the slender and long spines of lower part of lateral lobe of pronotum which slightly upward in male, and almost straight in female, and the shaped of female subgenital plate with shallow transversal creases. The updated key to all species occurring in Thailand is provided here.

Curcuminoid Contents in Rhizomes of Some Zingiberaceous Plants Sold via Online Platforms: Influence of Species and Cultivation Location.

Zingiberaceous plants are versatile and find application in various fields, including food, medicine, and cosmetics. Recently, turmeric and other Zingiberaceous plants have become readily available through online platforms. However, the quality, specifically the curcuminoid content, has not been adequately assessed. In light of this issue, this study is aimed at analyzing the curcuminoid contents, including bisdemethoxycurcumin, demethoxycurcumin, and curcumin, using high-performance liquid chromatography. The analysis targets the rhizomes of Zingiber montanum (ZM), Curcuma aromatica (CA), Curcuma wanenlueanga (CW), Curcuma zedoaria (CZ), and sixteen Curcuma longa (CL) samples sold on online platforms. The influence of species and cultivation locations was evaluated, compared, and clustered. The results indicated that CL exhibited the highest curcuminoid contents, followed by CA, CZ, ZM, and CW, respectively. Curcumin was not detected in CW, while bisdemethoxycurcumin and demethoxycurcumin were absent in ZM. Cluster analysis revealed that CW was closely related to ZM, and CA was closely related to CZ, while CL was not closely related to either cluster. Among the sixteen CL samples, the most commonly found curcuminoids were curcumin, followed by bisdemethoxycurcumin and demethoxycurcumin, respectively. Three samples contained curcuminoid contents of less than 5%, failing to meet the standard level specified in the Thai Herbal Pharmacopoeia. Furthermore, ten samples had total curcuminoid contents higher than 10%, with three samples exceeding 15%. The top three samples with the highest total curcuminoid contents from different locations were as follows: Tha Yang District, Phetchaburi Province (17.02%); Phop Phra District, Tak Province (16.97%); and Pak Tho District, Ratchaburi Province (15.45%). Cluster analysis revealed that CL samples could be grouped into two major categories: low curcuminoid and high curcuminoid groups. This study offers valuable insights for consumers seeking high-quality rhizomes of Zingiberaceous plants with high curcuminoids, through online platforms. By focusing on the curcuminoid content, consumers can make informed decisions when purchasing Zingiberaceous plants online. This information not only aids in selecting superior quality rhizomes but also enhances the overall consumer experience by ensuring the potency and efficacy of the purchased products.

Prevalence and risk factors of Toxoplasma gondii infection in dairy cattle from the Western Region of Thailand.

In total, 901 dairy cow sera and data were collected from 51 farms in Nakhon Pathom, Ratchaburi and Kanchanaburi provinces (Western Region of Thailand). Serum samples were processed via the multispecies ELISA method to detect IgG antibodies against Toxoplasma gondii infection. The results demonstrated that the calculated true prevalence was 1.48% (95% CI, 0.64-2.75%) for the individual-level and 29.41% (95% CI, 18.71-43%) for the farm-level. The univariate risk factor analysis showed that the number of total owned cats, the presence of stray cats, and the frequency of cleaning per day were significant factors (p<0.2). These three factors were subjected to logistic regression analysis, and the results revealed that the frequency of cleaning farms per day was a potential risk factor for T. gondii-seropositive farms (OR=2.745, 95% CI, 1.15-8.69, p=0.02). The frequency of cleaning might increase the T. gondii oocyst distribution within the barn area, thus increasing the possibility of infection. Our findings show that T. gondii continues to circulate in the dairy cow population in the western part of Thailand. The presence of cats on farms was not found to be associated with T. gondii infection, but the high frequency of cleaning the floor was, and contributed to the potential risk of infection.

Soil Carbon Stock and Soil Properties under Different Land Use Types of Agriculture

Agriculture soils play a crucial role in carbon storage and food security. However, uncertainty remains about soil carbon stocks due to spatial variability. This study estimated soil carbon stocks in agricultural land and examined the impact of land use and soil properties on soil organic carbon in Ratchaburi Province, Thailand. Soil samples were collected at three depths (0-10, 10-20, and 20-30 cm) within five different land use types: cassava, coconut, paddy fields, pineapple, and sugarcane. The results revealed that soil organic carbon decreased with increasing depth. Significant differences in soil carbon and soil properties were observed among land uses. The carbon stocks at 0-30 cm depth were as follows: coconut (35.87 mg C/ha), paddy fields (31.17 mg C/ha), sugarcane (28.02 mg C/ha), pineapple (21.79 mg C/ha), and cassava (16.12 mg C/ha). The carbon stocks were significantly correlated with sand, density, clay, silt, and pH. This study highlights the impact of land use types on carbon stocks in agricultural soils and emphasizes the role of soil properties, particularly soil texture, in influencing carbon storage variability. Furthermore, the study highlights the carbon storage potential in agricultural areas, which could guide the formulation of policies to utilize agricultural land to offset CO2 emissions from other sectors.

Development of Multi Language Communication in Local People using Electronic Dictionary

Research objectives were to develop e-dictionary in Thai, Lao Vieng, Mon, English and Chinese language to enchance cultural tourism communication in Ban Pong District, Ratchaburi Province, and to evaluate the e-book effectiveness. The research methodology is divided into four main parts: 1. development of linguistic content using focusing groups of 10 people with cultural and language knowledge of nationalities to analyze important cultural content and the translation of cultural content at the level of words, conversation and description from Thai to Lao Vieng, Mon, English and Chinese languages, and 2. developing an e-book with a multimedia appearance. The findings include: 1. The content of the book is divided into 5 chapters: Chapter 1 local food and desserts, Chapter 2 clothes, Chapter 3 local products, Chapter 4 cultural attractions, and Chapter 5 cultural activities with efficiency of 80.4/80.33.

Predicting Adverse Pregnancy Outcomes based on Maternal Serum Markers in Quad Test

OBJECTIVE To compare adverse pregnancy outcomes (APOs) between low-risk and high-risk serum quadruple test for aneuploidy screening. METHODS This cohort design study screened pregnant women who had undergone quad test serum screening in Ratchaburi province, Thailand. The sample of 400 women was divided into two groups, low-risk quad test (n=200) and high-risk quad test (n=200) using random allocation software. Participants were followed up for final outcomes and compared for APOs. The data were analyzed using binary logistic regression analysis and risk ratios. RESULTS Of a total of 1,891 pregnant women, 206 (10.8%) had APOs. Of the women with APOs, 92 (46%) had high-risk quad test results and 32 (16%) were in the low-risk quad test group. The incidence of preeclampsia, intrauterine growth restriction (IUGR) and chromosome abnormality in the high-risk quad test group were all statistically significantly higher in the high-risk quad test group than in the low-risk quad test group (p = 0.013, 0.001, 0.043 respectively). The high-risk quad test group also had a higher incidence of preterm birth, abortion, gestational diabetic mellitus in high-risk quad test group, but the differences were non-statistically significant. CONCLUSIONS High-risk serum quad test results can help predict the risk of APOs such as preeclampsia, IUGR and chromosome abnormality. KEYWORDS quad test, maternal serum marker, adverse pregnancy outcome

Contribution of phenolics to the antioxidant potential of propolis from Tetragonula laeviceps and Tetragonula pegdeni and its correlation to the dominant plant sources in different regions in Thailand

Propolis is a natural product produced by bees and has antioxidant properties that depend on the local flora and the bee species. Propolis from two dominant stingless bee species in Thailand, Tetragonula laeviceps and Tetragonula pegdeni were collected from four locations in Ratchaburi province and one location in Chiangmai province, with different dominant plants at each location. Samples were extracted in methanol (MeOH) and sequentially partitioned with hexane, dichloromethane (DCM), and MeOH. All partitioned extracts were examined by three antioxidant methods: 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and ferric reducing ability (FRAP) assays. For the DPPH, the MeOH-partitioned extract of T. laeviceps from Bankha district (MPE7) was the most active [50% effective concentration (EC50) of 75.62 µg/mL), while the DCM-partitioned extract of T. pegdeni from Suan phueng district (DPE2) was the most active in the ABTS and FRAP assays (EC50 of 22.06 µg/mL and 10.23 mM/g, respectively). DPE2 also showed the highest phenolic and flavonoid contents (98.94 µg gallic acid equivalents/mg dry weight and 286.07 µg quercetin equivalents/mg, respectively). High performance liquid chromatography analysis revealed the identification and quantification of 12 phenolic compounds, with the highest concentration of vitexin in MPE7 and MPE5. Interestingly, kaempferol (5.16 µg/mg) was only detected in DPE2. The different antioxidant properties among sites are expected due to the specific flora in each location. Furthermore, the bioactivity of propolis extracts differs significantly between bee species in a similar location, suggesting that they have different flora preferences. These results verify the potential of extracts to effectively scavenge radicals.

A Novel Simian Adenovirus Associating with Human Adeno-virus Species G Isolated from Long-Tailed Macaque Feces.

Metagenomics has demonstrated its capability in outbreak investigations and pathogen surveillance and discovery. With high-throughput and effective bioinformatics, many disease-causing agents, as well as novel viruses of humans and animals, have been identified using metagenomic analysis. In this study, a VIDISCA metagenomics workflow was used to identify potential unknown viruses in 33 fecal samples from asymptomatic long-tailed macaques (Macaca fascicularis) in Ratchaburi Province, Thailand. Putatively novel astroviruses, enteroviruses, and adenoviruses were detected and confirmed by PCR analysis of long-tailed macaque fecal samples collected from areas in four provinces, Ratchaburi, Kanchanaburi, Lopburi, and Prachuap Khiri Khan, where humans and monkeys live in proximity (total n = 187). Astroviruses, enteroviruses, and adenoviruses were present in 3.2%, 7.5%, and 4.8% of macaque fecal samples, respectively. One adenovirus, named AdV-RBR-6-3, was successfully isolated in human cell culture. Whole-genome analysis suggested that it is a new member of the species Human adenovirus G, closely related to Rhesus adenovirus 53, with evidence of genetic recombination and variation in the hexon, fiber, and CR1 genes. Sero-surveillance showed neutralizing antibodies against AdV-RBR-6-3 in 2.9% and 11.2% of monkeys and humans, respectively, suggesting cross-species infection of monkeys and humans. Overall, we reported the use of metagenomics to screen for possible new viruses, as well as the isolation and molecular and serological characterization of the new adenovirus with cross-species transmission potential. The findings emphasize that zoonotic surveillance is important and should be continued, especially in areas where humans and animals interact, to predict and prevent the threat of emerging zoonotic pathogens.

Cultural Adaptation of Thai: A Case Study of Chinese Descent in the Mae Klong River Basin

The purpose of this study is to investigate the cultural change of Thai-Chinese people in Muang District, Ratchaburi Province, as well as to investigate the factors that contribute to their cultural adaptation. Participants from the Mae Klong River Basin in Ratchaburi Province were interviewed, data analyzed, and descriptive writing was also used as a research method. The results indicate that cultural changes in Thai-Chinese has been changing from the past to the present, and it is constantly changing. Cultural change can be fast or slow, depending on the environment and social context. As human behavior is determined by culture, and culture governs society, today's culture has evolved according to the times, and today's communication is becoming more open. As the Internet and technology have made communication easier and faster, more foreign cultures are spreading across the globe. Moreover, Thai-Chinese cultural adaptation has been affected by a variety of factors over time, including population factors, economic factors, social and political factors. People of Chinese descent in Thailand are affected by societal and political factors that affect their adaptation. The use of social media and online resources is becoming increasingly modern, which has resulted in more effective communication between people in different locations. The findings of this study can provide suggestions for the development of the community in the future

Immunogenicity of the pentavalent DTwP-HB-Hib vaccine (Shan-5) used in the Thai Expanded Program on Immunization compared to the hexavalent DTaP-HB-Hib-IPV and DTwP-HB-Hib (Quinvaxem) vaccines administered to infants at 2, 4, 6 months of age

BackgroundThe pentavalent DTwP-HB-Hib (Shan-5) vaccine was first introduced into the Thailand Expanded Program on Immunization (EPI) in 2019. The Shan-5 vaccine is administered to infants at 2, 4, and 6 months of age, after initial vaccination with monovalent hepatitis B (HepB) and Bacillus Calmette-Guérin (BCG) vaccines at birth. This study compared the immunogenicity of the HepB, diphtheria, tetanus, and Bordetella pertussis antigens incorporated in the EPI Shan-5 vaccine versus the optional pentavalent (DTwP-HB-Hib) Quinvaxem and hexavalent (DTaP-HB-Hib-IPV) Infanrix-hexa vaccine. MethodsThree-dose Shan-5-vaccinated children were prospectively enrolled at the Regional Health Promotion Centre 5, Ratchaburi province, Thailand, between May 2020 and May 2021. Blood sampling was performed at months 7 and 18. The levels of HepB surface antibody (anti-HBs), anti-diphtheria toxoid (DT) IgG, anti-tetanus toxoid (TT) IgG, and anti-pertussis toxin (PT) IgG were evaluated using commercially available enzyme-linked immunoassays. ResultsAnti-HBs levels of ≥10 mIU/mL were achieved in 100 %, 99.2 %, and 99.2 % of infants in the Shan-5 EPI group, hexavalent group and Quinvaxem group one month after four dose immunization (at 0, 2, 4, 6 months of age), respectively. The geometric mean concentrations of the EPI Shan-5 and hexavalent groups were comparable but were higher than those of the Quinvaxem group. At one month after primary vaccination (month 7), infants in the Shan-5 EPI group had significantly higher levels of anti-DT IgG, anti-TT IgG, and anti-PT IgG than infants in the hexavalent and Quinvaxem groups. ConclusionsThe immunogenicity of the HepB surface antigen in the EPI Shan-5 vaccine was similar to that achieved by the hexavalent vaccine, but was higher than that achieved by the Quinvaxem vaccine. The Shan-5 vaccine is highly immunogenic and generates robust antibody responses after primary immunization.