Segregation bands (SBs) in quenching and partitioning (Q&P) steel plates will increase hydrogen embrittlement (HE) sensitivity, posing a major challenge in industrial production. This study addresses the issue by optimizing the microstructure of SBs through an additional double rapid tempering (DRT) heat treatment. The treatment effectively optimized the microstructure of the SBs, transforming combined martensitic islands into a mixed composition comprising isolated martensite islands, ferrite, and reverted austenite. This microstructure optimization of SBs altered the hydrogen crack nucleation characteristics from the thickness center of the steel plate to the corners of the specimen. Such a simple process significantly alleviates the adverse effects of SBs on HE sensitivity, providing a valuable reference for industrial applications.