Rapid Geographic Expansion Research Articles

Phylodynamic Insights into Global Emergence and Diversification of the Tomato Pathogen Xanthomonas hortorum pv. gardneri.

The emergence of plant pathogens is often associated with waves of unique evolutionary and epidemiological events. Xanthomonas hortorum pv. gardneri is one of the major pathogens causing bacterial spot disease of tomatoes. After its first report in the 1950s, there were no formal reports on this pathogen until the 1990s, despite active global research on the pathogens that cause tomato and pepper bacterial spot disease. Given the recently documented global distribution of X. hortorum pv. gardneri, our objective was to examine genomic diversification associated with its emergence. We sequenced the genomes of X. hortorum pv. gardneri strains collected in eight countries to examine global population structure and pathways of emergence using phylodynamic analysis. We found that strains isolated post-1990 group by region of collection and show minimal impact of recombination on genetic variation. A period of rapid geographic expansion in X. hortorum pv. gardneri is associated with acquisition of a large plasmid conferring copper tolerance by horizontal transfer and coincides with the burgeoning hybrid tomato seed industry through the 1980s. The ancestry of X. hortorum pv. gardneri is consistent with introduction to hybrid tomato seed production and dissemination during the rapid increase in trade of hybrid seeds. [Formula: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.

DNA methylase 1 influences temperature responses and development in the invasive pest Tuta absoluta.

DNA methylase 1 (Dnmt1) is an important regulatory factor associated with biochemical signals required for insect development. It responds to changes in the environment and triggers phenotypic plasticity. Meanwhile, Tuta absolutaMeyrick (Lepidoptera: Gelechiidae)-a destructive invasive pest-can rapidly invade and adapt to different habitats; however, the role of Dnmt1 in this organism has not been elucidated. Accordingly, this study investigates the mechanism(s) underlying the rapid adaptation of Tuta absoluta to temperature stress. Potential regulatory genes were screened via RNAi (RNA interference), and the DNA methylase in Tuta absoluta was cloned by RACE (Rapid amplification of cDNA ends). TaDnmt1 was identified as a potential regulatory gene via bioinformatics; its expression was evaluated in response to temperature stress and during different development stages using real-time polymerase chain reaction. Results revealed that TaDnmt1 participates in hot/cold tolerance, temperature preference and larval development. The full-length cDNA sequence of TaDnmt1 is 3765 bp and encodes a 1254 kDa protein with typical Dnmt1 node-conserved structural features and six conserved DNA-binding active motifs. Moreover, TaDnmt1 expression is significantly altered by temperature stress treatments and within different development stages. Hence, TaDnmt1 likely contributes to temperature responses and organismal development. Furthermore, after treating with double-stranded RNA and exposing Tuta absoluta to 35°C heat shock or -12°C cold shock for 1 h, the survival rate significantly decreases; the preferred temperature is 2°C lower than that of the control group. In addition, the epidermal segments become enlarged and irregularly folded while the surface dries up. This results in a significant increase in larval mortality (57%) and a decrease in pupation (49.3%) and eclosion (50.9%) rates. Hence, TaDnmt1 contributes to temperature stress responses and temperature perception, as well as organismal growth and development, via DNA methylation regulation. These findings suggest that the rapid geographic expansion of T absoluta has been closely associated with TaDnmt1-mediated temperature tolerance. This study advances the research on 'thermos Dnmt' and provides a potential target for RNAi-driven regulation of Tuta absoluta.

Chikungunya infection in returned travellers: results from the geosentinel network, 2005-2020.

Chikungunya is an important travel-related disease because of its rapid geographical expansion and potential for prolonged morbidity. Improved understanding of the epidemiology of travel-related chikungunya infections may influence prevention strategies including education and vaccination. We analysed data from travellers with confirmed or probable chikungunya reported to GeoSentinel sites from 2005 to 2020. Confirmed chikungunya was defined as a compatible clinical history plus either virus isolation, positive nucleic acid test or seroconversion/rising titre in paired sera. Probable chikungunya was defined as a compatible clinical history with a single positive serology result. 1202 travellers (896 confirmed and 306 probable) with chikungunya were included. The median age was 43years (range 0-91; interquartile range [IQR]: 31-55); 707 (58.8%) travellers were female. Most infections were acquired in the Caribbean (28.8%), Southeast Asia (22.8%), South Central Asia (14.2%) and South America (14.2%). The highest numbers of chikungunya cases reported to GeoSentinel were in 2014 (28.3%), 2015 (14.3%) and 2019 (11.9%). The most frequent reasons for travel were tourism (n = 592; 49.3%) and visiting friends or relatives (n = 334; 27.7%). The median time to presentation to a GeoSentinel site was 23days (IQR: 7-52) after symptom onset. In travellers with confirmed chikungunya and no other reported illnesses, the most frequently reported symptoms included musculoskeletal symptoms (98.8%), fever/chills/sweats (68.7%) and dermatologic symptoms (35.5%). Among 917 travellers with information available, 296 (32.3%) had a pretravel consultation. Chikungunya was acquired by international travellers in almost 100 destinations globally. Vector precautions and vaccination where recommended should be integrated into pretravel visits for travellers going to areas with chikungunya or areas with the potential for transmission. Continued surveillance of travel-related chikungunya may help public health officials and clinicians limit the transmission of this potentially debilitating disease by defining regions where protective measures (e.g. pretravel vaccination) should be strongly considered.

The distribution of the red-throated ascidian Herdmania momus shifts northwards in association with ocean warming in the Korean Peninsula

Abstract Climate change poses a major threat to marine ecosystems, with its effects felt worldwide. A major effect of climate change on marine ecosystems is the rise in water temperature, leading to a northward expansion of habitats for marine organisms. Herdmania momus, a species of ascidians (sea squirts), originally found in tropical and subtropical regions, was introduced to the Korean Peninsula. In this study, we examined the habitat of H. momus along the southeastern coast of the Korean Peninsula between 2016 and 2022. We found that H. momus settlements were observed across the entire survey area, with confirmed habitation in Busan in 2016, Ulsan in 2021, and Gyeongju (the northernmost location) in 2022. The observed habitation trend indicates a rapid geographical expansion, occurring approximately 79 years earlier than previously predicted. These observations demonstrate that marine organisms are undergoing a more rapid geographical expansion than previously projected. These unexpected findings should inform government policies related to proactive measures and strategies for managing the impact of climate change on marine ecosystems.

Update on the Epimed Monitor Adult ICU Database: 15 years of its use in national registries, quality improvement initiatives and clinical research.

In recent decades, several databases of critically ill patients have become available in both low-, middle-, and high-income countries from all continents. These databases are also rich sources of data for the surveillance of emerging diseases, intensive care unit performance evaluation and benchmarking, quality improvement projects and clinical research. The Epimed Monitor database is turning 15 years old in 2024 and has become one of the largest of these databases. In recent years, there has been rapid geographical expansion, an increase in the number of participating intensive care units and hospitals, and the addition of several new variables and scores, allowing a more complete characterization of patients to facilitate multicenter clinical studies. As of December 2023, the database was being used regularly for 23,852 beds in 1,723 intensive care units and 763 hospitals from ten countries, totaling more than 5.6 million admissions. In addition, critical care societies have adopted the system and its database to establish national registries and international collaborations. In the present review, we provide an updated description of the database; report experiences of its use in critical care for quality improvement initiatives, national registries and clinical research; and explore other potential future perspectives and developments.

Implementing a rapid geographic range expansion - the role of behavior changes

Implementing a rapid geographic range expansion - the role of behavior changes

Behavioral changes in the rapid geographic expansion of the great-tailed grackle

Behavioral changes in the rapid geographic expansion of the great-tailed grackle

India’s Cotton Balance Sheet under Two Decadal Scenarios: A Review

India ranks second in the global cotton balance, next only China. Cotton is the mainstay of India’s textile industry, accounting for roughly 74% of total textile mill usage. India’s cotton production has increased due to the introduction and rapid geographical expansion of bollworm resistant Bt cotton. The objective of this paper is to compare cotton balance analyses and to investigate the factors that have influenced cotton development over the last 22 years. The pertinent arguments presented in this article support the analytical discussion of the cotton trade’s yearly trends. Between 2000-01 and 2021-22, there will be an average of 30 million bales of cotton produced, 5.6 million exported and 1.4 million imported by India. By adding observable carryover stocks, the entire supply of cotton in India has consistently outperformed the total demand. However, Indian cotton farmers face a variety of issues, including increasing production costs, unmanageable debts, stagnant yields, constant pesticide use, inadequate irrigation, a lack of modern technology, manual picking, vulnerability to contamination, deterioration in genetic purity, competition from artificial fibres, particularly synthetic fibre, fluctuating market prices and lack of CCI participation. To boost cotton output, governments must provide high-yielding, high-quality hybrid seeds and appropriate irrigation infrastructure.

Infection Experiments Indicate That Common Florida Anurans and Lizards May Serve as Intermediate Hosts for the Invasive Pentastome Parasite, Raillietiella orientalis

Raillietiella orientalis, an invasive pentastome parasite, inhabits the lungs of snakes as adults and is rapidly spreading in Florida and Australia. The species that serve as intermediate hosts are currently unknown. We conducted laboratory infection experiments with insects (Blaberus discoidalis), lizards (Anolis sagrei), and anurans (Anaxyrus terrestris, Lithobates sphenocephalus, Osteopilus septentrionalis) to develop an understanding of which species may serve as intermediate hosts and to determine the fitness consequences of infection by R. orientalis. Lizards and insects, but not anurans, were readily infected by consuming food that was contaminated with pentastome eggs. Anolis sagrei and L. sphenocephalus were both infected after eating a single roach that was infected with R. orientalis larvae. Comparison with uninfected control animals revealed that pentastome infection did not significantly affect survival or growth in roaches, lizards, or anurans. The life cycle of R. orientalis in Florida is likely to involve a sequence of three hosts, with eggs hatching in coprophagous insects that infect lizards and anurans that infect the definitive host (snakes) after they are consumed. Our results indicate that the native species that serve as intermediate hosts are unlikely to experience major negative consequences from R. orientalis, unlike the native snake species that serve as the definitive hosts. The diversity of species that can serve as intermediate hosts and the potential for vehicular rafting by infected roaches and anoles indicate that the rapid geographic range expansion of R. orientalis will probably continue.

Interleukin 6: A biomarker for COVID-19 progression

COVID-19 was discovered in China for the first time in December 2019 and was declared a pandemic by the World Health Organization on March 11, 2020. Due to its rapid geographic expansion over the last three years, it has now become a global health issue. The infection is caused by SARS-CoV-2, which is obtained from a zoonotic source and transmitted directly or through contact. Following exposure, the immune system becomes hyperactive resulting in the production of pro-inflammatory cytokines, particularly interleukin-6 (IL-6), a naturally occurring pleiotropic cytokine that plays a significant role in respiratory failure and multi-organ dysfunction. This massive inflammatory phenomenon is reminiscent of cytokine release syndrome (CRS) or “cytokine storm”, which may be at the root of many severe complications. In fact, serum IL-6 levels are significantly high in patients with severe Covid-19 disease. The goal of treatment is to change the cytokine's concentration or activity. Interleukin-6 production could be inhibited, reducing inflammation and so serving as a therapeutic target. anti-interleukin-6 receptor monoclonal antibodies have been proven to reduce the severity of COVID-19 in clinical trials aimed at clarifying the function of immunoregulation. As a result, the Il-6 assay is a reliable predictor of morbidity and mortality at the time of infection diagnosis. The aim of our study is to highlight the role of interleukin 6 as biomarker of the COVID- 19 progression.

Metagenomic Characterization of Resistance Genes in Deception Island and Their Association with Mobile Genetic Elements.

Antibiotic resistance genes (ARGs) are undergoing a remarkably rapid geographic expansion in various ecosystems, including pristine environments such as Antarctica. The study of ARGs and environmental resistance genes (ERGs) mechanisms could provide a better understanding of their origin, evolution, and dissemination in these pristine environments. Here, we describe the diversity of ARGs and ERGs and the importance of mobile genetic elements as a possible mechanism for the dissemination of resistance genes in Antarctica. We analyzed five soil metagenomes from Deception Island in Antarctica. Results showed that detected ARGs are associated with mechanisms such as antibiotic efflux, antibiotic inactivation, and target alteration. On the other hand, resistance to metals, surfactants, and aromatic hydrocarbons were the dominant ERGs. The taxonomy of ARGs showed that Pseudomonas, Psychrobacter, and Staphylococcus could be key taxa for studying antibiotic resistance and environmental resistance to stress in Deception Island. In addition, results showed that ARGs are mainly associated with phage-type mobile elements suggesting a potential role in their dissemination and prevalence. Finally, these results provide valuable information regarding the ARGs and ERGs in Deception Island including the potential contribution of mobile genetic elements to the spread of ARGs and ERGs in one of the least studied Antarctic ecosystems to date.

Review of Housing Policies in Kosovo from 1947 to 2021

In a period of transition, sudden and numerous economic, social and political changes led to an uncontrolled growth of cities in Kosovo. The rapid population growth and geographical expansion of cities challenged planned development, which resulted in an uncontrolled urban expansion. This paper includes a historical review of housing policies in Kosovo from 1947 until 2021. The research starts with the period after World War II, which marked the beginning of a trend of rapid construction all over Europe, both due to urban growth and as a result of the destruction of many residential buildings. It analyses the implementation of policies, laws, housing regulations in residential neighbourhoods and, in particular, multi-unit housing in Kosovo. The research also includes housing policies in some of the most developed countries in Europe, to give the reader a clearer understanding and comparison of European and Kosovar housing issues.

In silico and in vitro evaluation of imatinib as an inhibitor for SARS-CoV-2

The rapid geographic expansion of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the infectious agent of Coronavirus Disease 2019 (COVID-19) pandemic, poses an immediate need for potent drugs. Enveloped viruses infect the host cell by cellular membrane fusion, a crucial mechanism required for virus replication. The SARS-CoV-2 spike glycoprotein, due to its primary interaction with the human angiotensin-converting enzyme 2 (ACE2) cell-surface receptor, is considered a potential target for drug development. In this study, around 5,800 molecules were virtually screened using molecular docking. Five molecules were selected for in vitro experiments from those that reported docking scores lower than −6 kcal/mol. Imatinib, a Bcr-Abl tyrosine kinase inhibitor, showed maximum antiviral activity in Vero cells. We further investigated the interaction of imatinib, a compound under clinical trials for the treatment of COVID-19, with SARS-CoV-2 RBD, using in silico methods. Molecular dynamics simulations verified that imatinib interacts with RBD residues that are critical for ACE2 binding. This study also provides significant molecular insights on potential repurposable small-molecule drugs and chemical scaffolds for the development of novel drugs targeting the SARS-CoV-2 spike RBD. Communicated by Ramaswamy H. Sarma

An overview of Zika virus genotypes and their infectivity.

ABSTRACTZika virus (ZIKV) is an enveloped, single-stranded RNA arbovirus belonging to the genus Flavivirus. It was first isolated from a sentinel monkey in Uganda in 1947. More recently, ZIKV has undergone rapid geographic expansion and has been responsible for outbreaks in Southeast Asia, the Pacific Islands, and America. In this review, we have highlighted the influence of viral genetic variants on ZIKV pathogenesis. Two major ZIKV genotypes (African and Asian) have been identified. The Asian genotype is subdivided into Southwest Asia, Pacific Island, and American strains, and is responsible for most outbreaks. Non-synonymous mutations in ZIKV proteins C, prM, E, NS1, NS2A, NS2B, NS3, and NS4B were found to have a higher prevalence and association with virulent strains of the Asian genotype. Consequently, the Asian genotype appears to have acquired higher cellular permissiveness, tissue persistence, and viral tropism in human neural cells. Therefore, mutations in specific coding regions of the Asian genotype may enhance ZIKV infectivity. Considering that mutations in the genomes of emerging viruses may lead to new virulent variants in humans, there is a potential for the re-emergence of new ZIKV cases in the future.

Spatiotemporal Associations and Molecular Evolution of Highly Pathogenic Avian Influenza A H7N9 Virus in China from 2017 to 2021.

Highly pathogenic (HP) H7N9 avian influenza virus (AIV) emerged in China in 2016. HP H7N9 AIV caused at least 33 human infections and has been circulating in poultry farms continuously since wave 5. The genetic divergence, geographic patterns, and hemagglutinin adaptive and parallel molecular evolution of HP H7N9 AIV in China since 2017 are still unclear. Here, 10 new strains of HP H7N9 AIVs from October 2019 to April 2021 were sequenced. We found that HP H7N9 was primarily circulating in Northern China, particularly in the provinces surrounding the Bohai Sea (Liaoning, Hebei, and Shandong) since wave 6. Of note, HP H7N9 AIV phylogenies exhibit a geographical structure compatible with high levels of local transmission after unidirectional rapid geographical expansion towards the north of China in 2017. In addition, we showed that two major subclades were continually expanding with the viral population size undergoing a sharp increase after 2018 with an obvious seasonal tendency. Notably, the hemagglutinin gene showed signs of parallel evolution and positive selection. Our research sheds light on the current epidemiology, evolution, and diversity of HP H7N9 AIV that can help prevent and control the spreading of HP H7N9 AIV.

An Update on Cephenemyiosis in the European Roe Deer: Emergent Myiasis in Spain.

Simple SummaryCephenemyiosis is a myiasis developing in the nasal cavity and pharynx of roe deer; it is widely spread in the range of distribution of this ungulate in Europe. In the last decade, this myiasis is expanding its distribution along its southernmost and northernmost range margins in Europe; thus, it is of the utmost importance to unravel all the epidemiological and clinical aspects of this myiasis, as well as implementing surveillance measures including reliable and non-invasive diagnostic techniques to monitor its expansion and adaptation to different ecosystems and/or hosts and to reduce the negative impact on roe deer populations.Cephenemyia stimulator is a Palearctic species developing in the nasal cavity and pharynx of roe deer (Capreolus capreolus). It is widely spread in the range of distribution of this ungulate in Europe. Since the first report of C. stimulator in Spain in 2001, a rapid geographic expansion has been observed, first in the north of the country, with high prevalence and intensities of infestation that caused some mortal cases, and, lately, also in Extremadura and Andalucía, the southernmost populations of European roe deer. These observations suggest an adaptation of this parasite to different ecosystems of the Iberian Peninsula. Almost simultaneously, C. stimulator is also expanding its range to northern Europe, with the first cases being reported in Sweden. Thus, Cephenemyia stimulator may be an example of a parasite currently displaying distributional changes along its southernmost and northernmost range margins. Thus, it is of the utmost importance to unravel all the epidemiological and clinical aspects of this myiasis, as well as implementing surveillance measures including reliable and non-invasive diagnostic techniques to monitor its expansion and adaptation to different ecosystems and/or hosts and to reduce the negative impact on roe deer populations.

Legacies of the Yale Report of 1828 in Liberal Education

The Yale Report was published in 1828 as a response to the rapid geographic, economic, and educational expansion in early, nineteenth-century America. The Report defined the purpose of collegiate liberal education as one that should ‘lay the foundation of superior education.’ According to the Report, this was to be best achieved by disciplining and grounding the mind on the faculty of psychology. In this process, the Report left both positive and negative legacies which have had long-term impacts on liberal education. The Report defined liberal education as one that provides general basic theories and principles. In addition, by stressing the importance of a ‘thorough education,’ the Report not only laid the basis for general education in later centuries, but also distinguished liberal education from professional, or vocational education, and the role and function of colleges accordingly. Moreover, the Report made a distinction between undergraduate and graduate studies. However, this distinction made it possible for liberal education to be downgraded into basic education for the study of concentrations. Meanwhile, the overemphasis on universal mental faculties which is content-neutral made it possible for non-liberal disciplines to be included within the curriculum of liberal education, which in turn would lower the status of liberal education. In addition, teacher-centered faculty psychology enabled the college as the guardian who took the parental responsibilities to enforce prescribed curriculum on their students. Moreover, faculty psychology also enabled liberal education to accommodate the new entrepreneurial and business demands of a rapidly changing society and thereby opened a way for neo-republicanism to be realized in America at that time. However, ironically, the discipline of the mind which was originally a tool and a skill for liberal education became its purpose since the notion of mental faculties was content-free as well as value-free. As a result, liberal education lacked virtues, values, moral⋅ethical ends, and public good. This undesirable phenomenon would give a grave lesson to the liberal education in current Korea enthralled by the notion of ‘core competencies.’

Outbreak of the South American tomato leafminer, Tuta absoluta, in the Chinese mainland: geographic and potential host range expansion.

In 2017 Tuta absoluta was identified as an invasive species in China. Due to its rapid geographic expansion and the severe crop damage it causes, T. absoluta poses a serious threat to China's tomato production industry. To determine its geographic distribution and host range, intensive surveys and routine monitoring were conducted across the Chinese mainland between 2018 and 2019. The population colonization coefficient (PCC; ratio of colonized sites and prefectures) and population occurrence index (POI; ratio of infested host species and PCCs) were calculated. In northwestern China, T. absoluta populations established in Xinjiang exhibited a medium PCC value (~0.03). In southwestern China, populations in Yunnan and its five neighboring provinces exhibited high (~0.50 in Yunnan and Guizhou), or low (<0.02 in Guangxi, Sichuan, Hunan, and Chongqing) PCC values. In the Chinese mainland, infestations of four crop plant species (tomato, eggplant, potato, and Chinese lantern) and two wild plant species (black nightshade and Dutch eggplant) were identified; tomatoes were infested in every colonized province. Chinese lantern and Dutch eggplant are potentially novel hosts. Yunnan, Guizhou, and Xinjiang experienced the most serious damage (POI). In southwestern China, observed damage significantly decreased with increased distance from the first discovery site of T. absoluta to the farthest county of an infested province increased. T. absoluta populations are well-established and could potentially spread to other regions of China. The present study helps to inform the establishment of better pest management guidelines and strategies in China and tomato-producing regions worldwide. © 2021 Society of Chemical Industry.

SARS-CoV-2 diagnostic testing in Africa: needs and challenges

Novel Coronavirus (also called COVID-19, or 2019-nCoV, or SARS-CoV-2) is an unprecedented pandemic. As depicted by the upsurge of the number of SARS-CoV-2 cases and the rapid geographical expansion of the virus, it is evident that it is truly a global health threat and Africa is not an exception to this threat. As of March 31 2020, 5, 287 SARS-CoV-2 cases and 172 deaths have been reported in 48 African nations. However, underreporting of numbers of cases may be due to diagnostic insufficiency, low testing capacity and most of all the fragile healthcare system on the continent. This letter emphasizes the needs and challenges of SARS-CoV-2 diagnostic testing in Africa.

International Cooperation in the Fight against Infectious Diseases: a Case for Legal Epidemiology in Kenya

As of May 21, more than 323,256 people worldwide have died and counting of COVID-19, the highly infectious respiratory disease caused by the coronavirus. The number of people, who have tested positive for COVID-19, has exceeded 4.9 million and counting, according to data compiled by the World Health Organization (WHO).The coronavirus disease 2019 (COVID-19) outbreak in the WHO African Region has rapidly evolved over the past few weeks, as reflected by the significant upsurge in the number of cases, and the rapid geographical expansion of the disease. Since the last situation report by the Johns Hopkins University on 18 March 2020 (External Situation Report 3), 11 new countries in the WHO African Region including Angola, Cape Verde, Chad, Eritrea, Niger, Madagascar, Mali, Mauritius, Mozambique, Uganda, and Zimbabwe have confirmed 1 371 and counting COVID-19 cases. As of 25 March 2020, a cumulative total of 1 716 confirmed cases have been reported across 38 countries in the region, bringing the total number of reported deaths to 30, which corresponds to a case fatality ratio of 1.8% among reported confirmed cases.The most affected countries in the WHO African Region are: South Africa (5647 cases and counting), Algeria (4006 cases and counting), Burkina Faso (645 cases and counting), Senegal (933 cases and counting), Cameroon (1832 cases and counting), and Cote d’Ivoire (1275 and counting) [13].This paper seeks to lay a theoretical foundation for a broader intellectual discourse on the emerging challenges presented by pandemics while discussing and examining how law as a social determinant of health and a factor in the cause, distribution and prevention of disease and injury in populations within Africa and Kenya in particular can be utilized in understanding trends in health laws, study the impact and effectiveness of laws in health, informing and supporting best practices and developing an evidence base of what works in the prevention of infectious diseases and in particular COVID – 19 in Africa (Kenya).