Three-metal-core-shell nanoparticles (Au@Pt@PdNPs) providing excellent peroxidase-like activity were applied in lateral flow immunoassay (LFIA), designated as Au@Pt@Pd-LFIA, for detecting the nucleocapsid protein (NP) of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2). An Au@Pt@Pd-LFIA was developed for quantitatively testing of SARS-CoV-2 NP with a range 0.12-31.25ng/mL. The limit of detection (LOD) of Au@Pt@Pd-LFIA strip was 0.06ng/mL, which was 16-fold or eightfold more sensitive than that of the gold lateral flow immunoassay (Au-LFIA) and the gold flower flow immunoassay (AF-LFIA) strips, respectively. For detection of clinical samples from nasal swabs using teststrips, Au@Pt@Pd-LFIA had 84.09% sensitivity, 100% specificity, and 92.55% accuracy. In terms of detection time, the testing of Au@Pt@Pd-LFIA strip was 16min similar to Au-LFIA (15min) and AF-LFIA (10min), but much shorter than ELISA (2h). In conclusion, Au@Pt@Pd-LFIA isa sensitive, rapid, and simple test for quantitative detection of SARS-CoV-2 NP in nasal swab samples.
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