Rapid Detection Of Hydrogen Sulfide Research Articles

Ultrasensitive and rapid detection of hydrogen sulfide by an upconversion nanosensor NaYF4:Yb3+, Tm3+@NaYF4@Ag2O

Hydrogen sulfide (H2S), a third gas signaling molecule, is considered to play a vital role in the development and treatment of diseases. To elucidate the intricate role of H2S in the organism and its participation in disease processes, there is an urgent need to visualize the dynamics of H2S. However, most currently available molecular probes have limitations in terms of sensitivity, specificity, and precision. In this study, the safe and biocompatible upconversion nanosensor NaYF4:Yb3+, Tm3+@NaYF4 anchored with Ag2O was successfully fabricated for H2S detection with an ultralow detection limit at 0.93 ng/mL. NaYF4:Yb3+, Tm3+@NaYF4@Ag2S is formed through in situ sulfuration reaction, and second near-infrared (NIR-II) fluorescence can be recorded upon the presence of H2S under 808 nm excitation. The results demonstrate the exceptional detection linearity and high specificity for H2S quantification. Additionally, NaYF4:Yb3+, Tm3+@NaYF4@Ag2O possesses a safe nature in normal and cancer cells. This nanosensor presents a NIR fluorescence imaging strategy for highly sensitive and specific detection of H2S, which has promises to be a practical tool for biomedical applications.

Pure-water-soluble colorimetric chemosensors for highly sensitive and rapid detection of hydrogen sulfide: Applications to evaluation of on-site water quality and real-time gas sensors

Hydrogen sulfide (H2S) has adverse effects on humans in various routes through water and air. However, the versatile detection of hydrogen sulfide (H2S) gas in various environments is still challenging. Herein, we report a highly sensitive, rapid, and selective colorimetric chemosensor (IID1) for the detection of H2S in diverse conditions including solution in pure water, thin-film, dyed-fabric, and coated silica in ambient air. The H2S-induced chemical reaction of IID1 results in significant spectral changes in UV-Vis absorption, accompanied by a discernible color change from dark red to colorless. Moreover, IID1 exhibits excellent selectivity among the competitive analytes, with a detection limit of 1.12 ppb and a rapid detection time (<10 s), which is among the highest performances reported for colorimetric H2S chemosensors. The detection mechanism is systemically investigated based on the analyses of UV-Vis absorption, NMR, and mass spectra. Utilizing the high solubility of IID1 in pure water, we successfully evaluate on-site water (river, lake, stream, reservoir, and tap water) quality with excellent accuracy. Furthermore, we demonstrate H2S detection on paper strips and face masks using the IID1. Our results suggest that IID1 is a practical sensor platform for the efficient detection of H2S in various environments.

A NIR ratiometric fluorescent probe for the rapid detection of hydrogen sulfide in living cells and zebrafish

Hydrogen sulfide (H2S) acts as a gas transporter and cell protector and plays a role in a number of disorders and signaling processes. Given that the half-life of H2S in biological systems is between seconds and minutes, the development of rapid and accurate technologies for reliable monitoring H2S levels and dynamics in organisms is critical. However, it is still difficult to design innovative near-infrared fluorescent probes that can quickly and accurately detect H2S. Here, we constructed a novel NIR ratiometric fluorescent probe based on the “aldehyde group auxiliary strategy”, Cy-H2S, for the quantitative detection and precise imaging of H2S in living cells and zebrafish. Cy-H2S responded quickly (150 s) and was highly sensitive (0.179 μM) to H2S donor. Cy-H2S was further successfully employed to track endogenous H2S fluctuation in HCT116 cells and zebrafish and evaluated the release efficiency of the H2S prodrug in a NIR ratiometric imaging way. Cy-H2S has the potential to be used as a reliable indication of H2S levels in living cells and zebrafish, as well as an innovative and practical instrument for furthering the physiological research of H2S, which will encourage the creation of advanced NIR ratiometric probes for a variety of biological applications.

BSA-assisted ultrasound synthesis of water stable CsPbBr3 nanocrystals for sensitive fluorescent detection of hydrogen sulfide in human serum.

A bovine serum albumin (BSA)-assisted ultrasonication strategy was developed for the synthesis of CsPbBr3 nanocrystals (NCs) with stable fluorescence properties in aqueous solution. Such a preparation method is simple, fast and does not require complex equipment. The results show that the synthesized CsPbBr3 NCs are homogeneous in particle size and have good solubility and stability in water. The CsPbBr3 NCs have been utilized as fluorescence probe for rapid detection of hydrogen sulfide (H2S) in human serum. The reaction of H2S with the lead sites on the surface of CsPbBr3 NCs produces lead sulfide (PbS), resulting in the decrease of fluorometric intensity of CsPbBr3 NCs. Our designed fluorescent assay has a linear S2- detecting range of 10 ~ 800nM with a detection limit of 7.05nM. The assay was used to determineH2S in human serum with spiked recoveries ranging from 94.98% to 102.69%. This work opens new avenues for the application of halide lead perovskite in different biosensing areas.

Preparation and effective detection to H2S of PdO/ZnO/ZnS based sensor at low working temperature

To achieve rapid detection of hydrogen sulfide (H2S) gas at lower temperatures, We present a high-performance PdO/ZnO/ZnS-based H2S gas sensor, which was synthesized using a hydrothermal synthesis method in conjunction with an in situ sulfuration process and surface modification by PdO. Vacuum sintering to prevent high temperature oxidation of ZnS. The introduction of PdO not only makes the sheet ZnO/ZnS turn to spherical-like, but also increased the content of adsorbed oxygen. Compared with ZnO/ZnS sample, the PdO/ZnO/ZnS based sensor exhibited a higher response value of 212.4 to 10 ppm H2S at lower temperature of 100 °C, with response and recovery times of 2.9 and 1.7 min, respectively. In addition, excellent performance is demonstrated in terms of gas selectivity, repeatability and long-term stability during testing.

Rapid detection of hydrogen sulfide in vegetables and monosodium glutamate based on perylene supramolecular aggregates using an indicator displacement assays strategy

Hydrogen sulfide (H2S) has been clearly identified as a hazardous chemical pollutant that seriously affects food safety and human health. In order to develop a rapid, accurate and efficient H2S tracking method, this work propose a strategy based on indicator displacement assays (IDA). A water-soluble histidine-modified perylene diimide fluorescent probe was synthesized by a one-step method, and the probe can form supramolecular aggregates in the presence of Cd2+. There will be a fluorescence transformation of probe, caused by the change of the state of aggregation and adjusted by various concentration of S2-, which can achieve the fluorescence detection of S2-. The limit of detection is as low as 0.41 µmol/L. Particularly worth mentioning is that the probe in this work can be recycled for at least 5 times, which is environmentally friendly and economical. Finally, this method was applied in three kinds of vegetables and monosodium glutamate samples.

ESIPT Fluorescence Probe Based on Double-Switch Recognition Mechanism for Selective and Rapid Detection of Hydrogen Sulfide in Living Cells.

A novel fluorescence probe, HBTSeSe, was designed and synthesized for the detection of H2S with a double-switch mechanism of a broken diselenide bond followed by thiolysis of ether. Then, 2-(2′-hydroxyphenyl)benzothiazole (HBT) was released as fluorophore, which has large Stokes shift based on the excited state intramolecular proton transfer process. The probe responded selectively and rapidly to H2S, with the fluorescence increased by 47-fold immediately after the addition of H2S. HBTSeSe was able to detect H2S in the cytoplasm, specifically in cell imaging experiments. The results also showed that H2S was produced in the immune response of RAW264.7 cells activated by phorbol-12-myristate-13-acetate.

Triggered emission for rapid detection of hydrogen sulfide chaperoned by large Stokes shift

Current manuscript demonstrates the development of a reaction-based "turn-on" green emissive molecular probe (P4) for rapid detection of H2S in physiological condition. The probe was found to exhibit low limit of detection (1.19 μM), along with high selectivity, large Stokes shift and good quantum efficiency. The change in the optical behaviour of the probe was also theoretically scrutinized employing density functional theory calculations which stand in the strong agreement with the photophysical outcomes. Given the demand for large Stokes dyes for sensing applications mainly because of their special photophysical properties, and as the reported number is very limited, the present probe with 110 nm Stokes shift is a good addition in the area of large Stokes dyes for H2S recognition in physiological conditions.

Design, synthesis, crystal structure and cytotoxicity studies of colorimetric fluorescent “OFF-ON” probes for rapid detection of hydrogen sulfide based on Cu(II) complex

Four colorimetric fluorescent probes based on copper(II) complexes were synthesized and the molecular structures were characterized by X-ray diffraction, ESI-HRMS and elemental analysis. When exposed to HS−, the synthesized probes showed significant color changes from yellow to red and remarkable increase of fluorescence intensity (over 80-fold). What's more, the interaction of host-guest could be completed in 2 min. However, no clear color and fluorescence intensity changes were observed in the presence of other anions (AcO−, H2PO4−, F−, Cl−, Br−, I−, SO42−, SO32−, CO32−, Cys and GSH). The synthesized colorimetric fluorescent probes could detect HS− rapidly and conveniently. Cytotoxicity studies indicated that the synthesized fluorescent probes were low cytotoxicity in Hela cells and may be used to detect H2S level in vivo.

A nitroso-based fluorogenic probe for rapid detection of hydrogen sulfide in living cells

A turn-on fluorescent probe for hydrogen sulfide (H2S) employing flavylium derivative as the fluorophore and nitroso group as the recognition unit and was designed and synthesized. The free probe is almost non-fluorescent. Upon addition of H2S to the solution of the probe, its nitroso group was transformed to the corresponding amino group with fast reaction kinetics, affording a dramatic enhancement of fluorescence emission. The fluorescence intensity of the proposed probe at 625 nm showed a good linear with the concentration of NaHS over a range from 0 to 4 μM, with a limit detection of 29 nM (3δ). The proposed probe exhibited high selectivity, excellent sensitivity and rapid response (∼5 min) for H2S. Moreover, this probe was successfully used for fluorescence imaging of exogenous and endogenous H2S in living cells.

Rapid detection of hydrogen sulfide produced by pathogenic bacteria in focused growth media using SHS-MCC-GC-IMS

A new rapid method for the detection of hydrogen sulfide from pathogenic bacteria is reported. The developed method, static headspace – multi-capillary column - gas chromatography - ion mobility spectrometry (SHS-MCC-GC-IMS), has been applied to detect hydrogen sulfide evolution from 61 bacteria. The developed method has been compared against a standard triple sugar iron (TSI) agar approach, and a modified single sugar iron (SSI) agar approach. Hydrogen sulfide detection by SHS-MCC-GC-IMS using an initial inoculum of 1–1.5 × 105 CFU/mL can be achieved within 6 h, after incubation at 37 °C, with a limit of detection of 1.6 ng/mL. Data for the standard agar method against the new instrumental approach, and the modified agar method against the new instrumental approach, are compared. The specificity for the new method compared against the standard method and the modified agar approach across all 61 strains was 85.2% and 88.5% respectively, and 86.7% and 91.3% across the 23 Salmonella strains tested.

Reaction-based fluorescent probes for rapid detection of hydrogen sulfide in vivo

The probe exhibited high sensitivity, selectivity, and fast response for real-time detection of H2S in vivo.

A novel lysosome-targeted fluorogenic probe based on 5-triazole-quinoline for the rapid detection of hydrogen sulfide in living cells.

A fluorogenic probe based on the novel fluorophore 5-triazole-quinoline was developed for the detection of hydrogen sulfide, an endogenous signaling molecule associated with the development of various diseases. The lysosome-targeted probe Lyso-HS was synthesized via C-H direct azidation from 8-aminoquinoline; it was able to detect H2S in 1 min and exhibited excellent turn-on ability with 95-fold fluorescence enhancement based on a new fluorochrome. The high quenching efficiency was further verified using time-dependent density functional theory (TDDFT). The probe also exhibited high selectivity and a low detection limit (as low as 214.5 nM), which has practical applications for disease detection and monitoring.

A polyoxometalate-based supramolecular chemosensor for rapid detection of hydrogen sulfide with dual signals

Hydrogen sulfide (H2S) has been verified as an important biological mediator in human physiological activities, but its rapid and accurate detection is remaining a challenge. Based on our early work, Eu-containing polyoxometalate/ionic liquid-type gemini surfactant hybrid nanoparticles fabricated by EuW10O36·32H2O (Eu-POM) and 1,2-bis(3-hexadecylimidazolium-1-yl) ethane bromide ([C16-2-C16im]Br2) via ionic self-assembly (ISA) strategy, we modified the hybrids with copper (II) ion and used them as a novel turn-off supramolecular fluorescence probe for H2S immediate response. Although copper (II) ions can cause decrease of the fluorescence intensity, the probe with moderate amount of copper (II) still has a high performance in emission property. The copper (II) ion-modified supramolecular sensor (CSS) shows dual signals in the fluorescence intensity and absorbance for H2S detection, and the detection limit is about1.25μM. Furthermore, CSS displays high selectivity for H2S in the presence of other anions and species (e.g. Cl−, Br−, I−, SO42−, SO32−, S2O32−, AC−, H2O2, HCO3−, l-cysteine, homocysteine and l-glutathione), and also have potential for preferential imaging in vivo. Besides, the fluorescence quenching mechanism of CSS in the presence of H2S was explored. CuS generated by the reaction between Cu2+ and H2S was testified to act as a quencher, and the nonradiative resonance energy transfer mechanism was speculated to be responsible for fluorescence quenching. It is anticipated that the as-prepared CSS will be used as an efficient chemosensor for the rapid detection of H2S, which is critical for the diagnosis of some diseases, e.g. Alzhermer’s disease, Down’s syndrome, and diabetes, etc.

Dual Signaling Molecule Sensor for Rapid Detection of Hydrogen Sulfide Based on Modified Tetraphenylethylene

A dual signaling molecule sensor has received increasing attention owing to its ability to read out target analytes with more than one transduction channel and thus make the results more convincing. Here we have developed a dual signaling molecule sensor that is well suited for monitoring hydrogen sulfide (H2S) levels through fluorescence, UV-visible adsorption, and visual mode. Results showed the selective and instantaneous responses of sensor toward intracellular H2S. Moreover, the sensor was successfully applied to imaging of H2S levels in Caenorhabditis elegans (C. elegans) and observed the changes of H2S under starvation of C. elegans. Altogether, the sensor was proved to be a useful tool for tracking H2S levels in cells and in vivo. The merits of two kinds of independent signaling molecules allow us to select different output modes according to the different samples.

A remarkable ratiometric fluorescent chemodosimeter for very rapid detection of hydrogen sulfide in the vapour phase and living cells

A ratiometric fluorescent probe having a fast response and a large Stokes shift detects SH−both in solid and vapour phases and this probe is used for fluorescence imaging of SH−in living cells.

A highly sensitive and water soluble fluorescent probe for rapid detection of hydrogen sulfide in living cells

A highly water soluble fluorescent probe has been exploited for the rapid detection of hydrogen sulfide in live cells.

A fluorescent probe for rapid detection of hydrogen sulfide in blood plasma and brain tissues in mice

We describe the design, synthesis and application of a sensitive, selective, and fast fluorescent probe for H2S detection. A linear relationship between emission intensity and sulfide concentration was observed in biological bovine plasma systems. With this probe, we were able to estimate the sulfide concentration in mouse blood plasma and brain tissues.