The gas phase flow field inside a cyclone separator is crucial to the particle separation process. Previous studies have paid attention to the steady-state characteristics of the gas phase flow field, while research on its dynamic characteristics remains insufficient. Meanwhile, cyclone separators often adopt different structural forms according to the process requirements, the evolution laws of the dynamic characteristics flow field within them are still not well understood. Therefore, in this study, a hot-wire anemometer (HWA) was employed to measure the instantaneous tangential velocity of the gas phase flow fields within different structural cyclone separators (cylinder type, cylinder–cone (no hopper), and cylinder–cone (with hopper)). Comparative analyses and discussions were conducted regarding the dynamic characteristic distribution rules of the flow field in the time domain and the frequency domain. The results revealed that the dimensionless tangential velocity distributions of different types of cyclone separators all conformed to the Rankine vortex structure. The instantaneous tangential velocity fluctuated with low frequency and high amplitude, and the low-frequency velocity fluctuation exhibited a transfer behavior along the radial direction. Compared with the cylinder–cone-type cyclone separator, the tangential velocity in the cylinder-type cyclone separator fluctuated more greatly, and its quasi-periodic behavior was also more obvious. The time-averaged tangential velocity, the tangential velocity fluctuation intensity (Sd), and the dominant fluctuation frequency all had obvious attenuation along the axial direction in the cylinder-type cyclone separator, while the above-mentioned parameters had no attenuation along the axial direction in cylinder–cone-type cyclone separators. Additionally, the backflow from the hopper of the cylinder–cone-type cyclone separator (with hopper) led to an increase in the instantaneous tangential velocity fluctuation intensity of the local flow field near the dust outlet, as well as the occurrence of the “double dominant frequencies” phenomenon.