Escherichia coli concentrations of bottom sediment and overlying water were determined from a variety of streams in southwestern Idaho by a one-step most probable number technique. Results show E. coli concentrations of bottom sediments to be from 2 to 760 times greater than from the overlying water. E. coliconcentrations of bottom sediment were found to be resuspended following disturbance simulation and a rainstorm event, contributing to pollution of the overlying waters. It is, therefore, suggested that microbial analysis of bottom sediments be considered a part of water-quality evaluations for rangeland streams. During the past decade, Federal legislative action has brought attention to sources of nonpoint pollution related to livestock grazing on public lands. Collectively, the National Environmental Policy Act of 1969, the Federal Water Pollution Control Act Amendments of 1972, and the Federal Land Policy and Management Act of 1976 have specified the need to establish criteria for identifying pollution sources and to improve environmental quality of streams through improved management. To achieve the goals set forth in these acts, many research programs have been initiated to identify and document pollution sources. One of the findings has been the implication that livestock grazing on western rangeland watersheds is a source of bacterial pollution of streams (Darling and Coltharp 1973; Doty and Hookano 1974; Buckhouse and Gifford 1976; Skinner et al. 1974; Authors are geologist, USDA ARS, 1175 South Orchard, Suite 116, Boise, Idaho 83705; and associate professor of microbiology, Boise State University, 1910 University Drive, Boise, Idaho 83725, respectively. This research is a contribution from the Northwest Watershed Research Center, Agricultural Research Service, U.S. Dep. Agr.; and Bureau of Land Management, U.S. Dep. Interior; in cooperation with the Agricultural Experiment Station, University of Idaho, Moscow 83843. The authors gratefully acknowledge the efforts of James Harris and Rebecca O'Brien, biological technicians, for their laboratory and field assistance during the course of this study. Manuscript received May 27, 1980. Stephenson and Street 1978). Researchers have had difficulty, however, explaining variations in bacterial indicators occurring in many of the studies (Buckhouse and Gifford 1976; Darling and Coltharp 1973; Kunkle 1970; Skinner et al. 1974; Stephenson and Street 1978). These variations can be characterized by two questions: (1) why do fecal coliform counts remain relatively high in some streams after livestock have been removed from the area, and (2) what is the source of sudden fecal coliform increases in stream runoff from rainstorms or snowmelt? Wildlife have been suggested as the source in question one (Fair and Morrison 1967; Stuart et al. 1971; Walter and Bottman 1967; Doran and Linn 1979), while for question two, Stephenson and Street (1978) suggest that fecal coliforms remain in soil and adjacent streambanks to be flushed into the streams during subsequent runoff. In an attempt to more fully answer the above questions, we began a study in 1979 on southwestern Idaho rangeland, utilizing eight sampling sites on six separate shallow stream segments within varying land-use practices. Our objective was to evaluate stream bottom sediments as a possible reservoir of bacterial pollutants available to overlying surface waters via resuspension. In previous work relating possible bacterial pollutants to stream bottom sediment, none of the studies have dealt with rangeland environments. In a study of the Greenwater River Watershed in Washington, Varness et al. (1978) found that concentrations of fecal coliforms increased dramatically during periods of human use. Since rainfall and surface runoff were minimal, they suggested that fecal coliforms might be surviving in sediments. Matson et al. (1978) found mean fecal coliform counts in sediment to be 2,500 times greater than in the overlying water, upstream of a sewage treatment plant on the Shetucket River in northeastern Connecticut. Downstream of the treatment plant effluent discharge site, the fecal coliform counts for the sediment JOURNAL OF RANGE MANAGEMENT 35(1), January 1982 119 This content downloaded from 207.46.13.33 on Sun, 20 Nov 2016 04:17:32 UTC All use subject to http://about.jstor.org/terms Boise P?j 2 ~~Idaho City
Read full abstract