Mr Range Research Articles

Effect of Cd on Structural, Spectral, Morphological and Dielectric Properties of Sr1-xCdxZn2Fe4O11 R-type Hexaferrites Synthesized via Sol-gel Technique

Sol–gel method was used to prepare Sr1-xCdxZn2Fe4O11 (x = 0.00, 0.02, 0.06, 0.1) R-type hexaferrite. The synthesized materials were sintered at 850oC and desired phase was obtained. X-ray diffraction analysis confirms that R-type hexaferrite exists only as a single phase. Using the Scherer formula, crystallite size for all of the prepared samples was found to be in the range of 10.39–12.62 nm. Crystallite size (D), the lattice parameters (a, c), and the cell volume (Vcell), d-spacing, bulk density, X-ray density, porosity, dislocation density and micro strain were determined in structural analysis. Fourier transform infrared spectroscopy method was used to identify the metal-oxygen vibrations at different locations. FT-IR verifies the presence of the Fe–O stretching vibration band at 743 and 867 cm−1. The typical grain size in surface morphology investigation ranges from 0.56 to 0.82 μm. Dielectric response of ferrite ceramic samples replaced with Cd2+ was investigated in the frequency range of 1 MHz–3 GHz. The AC conductivity rises with an increase in frequency because they are proportional to one another. This increasing tendency is effectively described by the theory of Maxwell-Wagner and Koop. Q-values remain constant as frequency rises and behaves independently of frequency as long as frequency reaches 1.7 GHz. These types of materials are utilized in high-frequency applications including frequency filters and resonant circuits. All of the magnetic properties determined by analyzing the M − H loops, including saturation magnetization (Ms), retentivity (Mr), and coercivity (Hc), exhibit an increasing trend as the substitution of Cd2+ rises. Ms (49.76–56.38 emu/g), Mr (15.82–18.30 emu/g) and Hc range from 203.20 Oe to 215.80 Oe. Grain size decreases cause arise in coercivity, which is caused by an enhancement in magneto-crystalline anisotropy. Overall results suggest that Cd2+ replaced R-type hexagonal ferrites are a great resource for longitudinal recording media; they have the potential to be used in a wide variety of electronic applications, including resonant circuits and high-frequency filters, security, detecting and switching.

165 PHARMACIST-LED MEDICINES RECONCILIATION: AN OBSERVATIONAL STUDY TO EVALUATE INFORMATION SOURCES AS A QUALITY INDICATOR OF THE SERVICE

Abstract Background Medicines Reconciliation (MR) involves obtaining a Best-Possible Medication History (BPMH) and is essential for reducing medication errors and adverse events. Recommendations on how best to undertake MR range from utilising the patient alone to using ≥2 sources (in addition to the patient). Few studies evaluate the use of >1 source as a measure of MR quality. Methods This observational study was undertaken in a major teaching hospital that provides a dedicated pharmacist-led MR service. Eligible patients (aged ≥65 years, admitted through the Emergency Department) were randomly recruited. MRs were compiled using 2 sources and compared to individual 1-source MRs (both in addition to patient/carer interview). Additional Medication Discrepancies (AMDs), or differences between 1-source and 2-source MR, were assessed and potential predictors analysed. Results A total of 114 patients were included. Community pharmacy was the most frequently used and most accurate source (full agreement in 97.4% of BPMHs). Two-thirds of MRs would not differ if either of the two sources were used in isolation, alongside patient/carer interview. The remaining MRs identified a total of 85 AMDs (1–6 per MR). The majority were drug omissions (49.4%). Most were deemed “low” potential clinical significance (59%). Higher patient/carer reliability was associated with lower incidence (p=0.001) and fewer AMDs (p=0.001). Conclusion Contrary to some international guidelines, using >1 source (in addition to patient/carer interview) does not add value to the BPMH in the majority of cases.

Identification of Proteases and Protease Inhibitors in Seeds of the Recalcitrant Forest Tree Species Quercus ilex.

Proteases and protease inhibitors have been identified in the recalcitrant species Quercus ilex using in silico and wet methods, with focus on those present in seeds during germination. In silico analyses showed that the Q. ilex transcriptome database contained 2,240 and 97 transcripts annotated as proteases and protease inhibitors, respectively. They belonged to the different families according to MEROPS,1 being the serine and metallo ones the most represented. The data were compared with those previously reported for other Quercus species, including Q. suber, Q. lobata, and Q. robur. Changes in proteases and protease inhibitors alongside seed germination in cotyledon and embryo axis tissues were assessed using proteomics and in vitro and in gel activity assays. Shotgun (LC–MSMS) analysis of embryo axes and cotyledons in nonviable (NV), mature (T1) and germinated (T3) seeds allowed the identification of 177 proteases and 12 protease inhibitors, mostly represented by serine and metallo types. Total protease activity, as determined by in vitro assays using azocasein as substrate, was higher in cotyledons than in embryo axes. There were not differences in activity among cotyledon samples, while embryo axis peaked at germinated T4 stage. Gel assays revealed the presence of protease activities in at least 10 resolved bands, in the Mr range of 60–260 kDa, being some of them common to cotyledons and embryo axes in either nonviable, mature, and germinated seeds. Bands showing quantitative or qualitative changes upon germination were observed in embryo axes but not in cotyledons at Mr values of 60–140 kDa. Proteomics shotgun analysis of the 10 bands with protease activity supported the results obtained in the overall proteome analysis, with 227 proteases and 3 protease inhibitors identified mostly represented by the serine, cysteine, and metallo families. The combined use of shotgun proteomics and protease activity measurements allowed the identification of tissue-specific (e.g., cysteine protease inhibitors in embryo axes of mature acorns) and stage-specific proteins (e.g., those associated with mobilization of storage proteins accumulated in T3 stage). Those proteins showing differences between nonviable and viable seeds could be related to viability, and those variables between mature and germinated could be associated with the germination process. These differences are observed mostly in embryo axes but not in cotyledons. Among them, those implicated in mobilization of reserve proteins, such as the cathepsin H cysteine protease and Clp proteases, and also the large number of subunits of the CNS and 26S proteasome complex differentially identified in embryos of the several stages suggests that protein degradation via CNS/26S plays a major role early in germination. Conversely, aspartic proteases such as nepenthesins were exclusively identified in NV seeds, so their presence could be used as indicator of nonviability.

Meniscal Allograft Transplantation: results at 6-year Mean Follow-up

ObjectivesThe purpose of this study was to evaluate the clinical and imaging results in a series of patients who underwent a meniscal allograft transplant (MAT).MethodsA retrospective study was performed. The International Knee Documentation Committee (IKDC) score, Lysholm score, and physical examinations were reviewed to measure clinical outcomes after MAT. Osteoarthritic changes were assessed by Plain radiography according to Kellgren-Lawrence (KL) classification and meniscal extrusion by magnetic resonance imaging (MRI). Surgical technique and related procedures were analyzed. Complications were registered. The minimum follow-up was 2 years.ResultsSurgeries were performed in 41 patients, 36 fulfilled the inclusion criteria. Twenty-five were male and 11 women. The mean age was 35 years old (SD 8). The mean follow-up was 71 months (range 24 to 184). The mean time between meniscectomy and meniscal transplant was 12.8 years (SD 6,7). Twenty-three were lateral menisci.The following related procedures were performed: Seven ACL reconstructions, 3 ACL revisions, 19 mosaicplasties and 3 osteotomies. The mean preoperative results of IKDC and Lysholm scores were 30.3 (SD 15.5) and 43.8 (SD 16.8) respectively and postoperative results were 78.8 (SD 21.1) and 61.1 (SD 15) respectively.There were no significative radiographic changes at the end of the follow-up. Mean KL preoperative was 2 (IQR 1) and last follow up was 2 (IQR 1) p= 0.053. Preoperative KL grade 1: 33%, grade 2: 50% and grade 3: 17% and last follow up KL grade 1: 22%, grade 2 56% and grade 3 22%.The average percentage of extruded meniscus was 53% in 22 patients evaluated with MR (range 19% to 100%). Five presented arthrofibrosis that required mobilization under anesthesia and partial meniscectomy of the MAT was required in 6 patients. Two transplants failed.ConclusionAlthough 53% of meniscal extrusion was observed in the MR, we found no correlation with clinical outcomes. MAT achieved good clinical results without osteoarthritic changes after a mean of 6-years follow-up, with 5% of failure. MAT appears to be a safe and effective treatment, which should be considered as a suitable option in the clinical practice.

Building a load characteristic of the fuel injection system of a ship’s medium-speed engine diesel in dynamic tests conditions

The object of research in the work is hydrodynamic processes in the high pressure fuel system of the ship’s medium-speed engine on variable modes. Variable regimes constitute a significant part of the operational time of a number of types of ships (tugs, fishing vessels, etc.), and for transport fleets they are characteristic for maneuvering. In the latter case, reliability and environmental safety for sanitary control zones are of particular importance. Studies of fuel supply processes in variable modes are important, since they largely determine all the operating characteristics of a diesel engine and are not well studiedThe article presents an experiment in which the task is obtaining the load characteristics and the study of transients in the fuel equipment of the ship’s medium-speed diesel engine under dynamic testing conditions. In the course of the research, a developed hardware and software complex is used to ensure the implementation of the experiment plan, fixation, processing and oscillography of the obtained data. An electromechanical system is developed by rack displacement of a high-pressure fuel pump with programmed computer control. The experiment program provides for changing the rack position in the whole range covering the operational load characteristic. The discrete displacement includes five fixed rack positions with a step transition between them. During testing, the high-pressure fuel pump completes 80 injection cycles over 44 s. The transition time between the individual fixed positions of the racks is 0.44 s. The stabilization period of hydrodynamic processes in the fuel supply system is close to 0.22 s. In the investigated range of rack positions mr=25–5 mm the main parameters of the fuel injection are as follows: рn.с=474–232 bar; рn.i=457–222 bar; рp=445–162 bar.The possibility of obtaining load characteristics by dynamic tests is shown, which significantly reduces the test time and increases the reliability of the data, eliminating the influence of the time trend parameters.

Antiviral activity of prickly pear (Opuntia ficus-indica (L.) Miller) extract: Opuntin B, a second antiviral protein

We previously showed that aqueous extract of prickly pear (Opuntia ficus-indica) cladode has significant antiviral activity against Cucumber mosaic virus (CMV: family Bromoviridae). Antiviral activity of the extract was retained for a period of 9 days on treated faba bean leaves. This activity was considerably decreased at concentrations lower than 30 mg mL−1, and by washing the treated leaves of faba bean plants 2 h after application of cactus extract, and was ineffective when applied 24 h after virus inoculation. No inhibitory effect was found against agro-inoculated strain of Beet curly top virus (BCTV: family Geminiviridae) on faba bean plants. Prickly pear extract reduced the efficiency of CMV transmission by Aphis gossypii on treated cucumber seedlings. An antiviral protein designated Opuntin B was isolated from the extract by heat treatment and differential centrifugation, and bulk protein precipitation by ammonium sulfate followed by cation and anion exchange chromatography. SDS-PAGE analysis in the presence of 2-ME revealed that Opuntin B is made of four protein components, in the molecular weight (Mr) range of 29–32 kDa, while in the absence of 2-ME, only two bands in the range of 26–28 kDa were visible. Native PAGE of the protein also showed 2 bands. Purified Opuntin B completely inhibited CMV on faba bean and zucchini plants at concentration of 40 μg mL−1 and had an IC50 of 0.75 μg mL−1 for inhibition of the virus on faba bean leaves. The isolated antiviral protein exhibited inhibitory activity against Zucchini yellow mosaic virus (ZYMV: family Potyviridae) Squash mosaic virus (SqMV: family Secouviridae) and Tobacco mosaic virus, (TMV: family Virgaviridae), as well.

Systematic review on the characterization of chronic traumatic encephalopathy by MRI and MRS.

Chronic traumatic encephalopathy (CTE) is a neurodegenerative disease that is found in people who have suffered from chronic traumatic brain injury (TBI). Up to now, diagnosis of CTE could only be made based on postmortem histopathological examinations. The application of MR techniques might offer a promising possibility for in vivo diagnoses. To provide a critical systematic review of the characterization of chronic TBI and CTE by considering the range of MR techniques. This was a systematic review for which the electronic databases PubMed and Embase were searched using the terms ("chronic traumatic encephalopathy" OR "punch drunk syndrome" OR "chronic traumatic brain injury" OR "dementia pugilistica" OR "chronic head trauma") AND ("magnetic resonance imaging" OR mri OR imaging OR mrs OR "magnetic resonance spectroscopy" OR spectroscopy). Of the 432 studies identified by the database search, 25 were included in this review. Diffusion, structural, and functional MRI sequences and MR spectroscopy were evaluated at 1.5T or 3T and at 11.74T for the ex vivo studies. Data were extracted by two reviewers independently. Specific inclusion and exclusion criteria like the study design, publication type, and applied MR techniques were used to select studies for review. Results of the original research articles were stated in this review as significant if P ≤ 0.05. Of the included articles, two were ex vivo studies focusing on the coregistration of histology and MRI. All other studies were based on in vivo data. The included studies varied considerably regarding study setup, MR techniques, and results. Nevertheless, this work aims to establish links between the studies and discusses the results and limitations associated with the characterization of chronic TBI and CTE based on MR. 3 Technical Efficacy: Stage 2 J. Magn. Reson. Imaging 2019;49:212-228.

Laryngotracheal anomalies and airway fluoroscopy in infants

ObjectivesThe role of airway fluoroscopy in the diagnosis of laryngotracheal anomalies in infants is controversial. We aimed to (i) compare airway fluoroscopic characteristics with endoscopic findings in infants presenting for evaluation of upper airway obstruction and (ii) assess the as low as is reasonably achievable (ALARA) status for airway fluoroscopy as an initial diagnostic test in suspected laryngotracheal anomalies. Materials and methodsWe performed a retrospective review of children who underwent fluoroscopy and endoscopic evaluation of the airway in the operating room for suspected laryngotracheal anatomic abnormalities. Thirty-four infants who underwent both procedures at a tertiary level university-based children's hospital from January 1, 2008 to December 1, 2013 were included. Infants with suspected foreign bodies or an existing tracheostomy were excluded. Intraoperative findings from endoscopy and radiologic interpretation from fluoroscopy were compared using standard tools for validation of a diagnostic test. These metrics were compared with historic data that suggested good correlation between radiologic and endoscopic findings in older children. ResultsThe median age was 3.6 months (range 1–8 months). The sensitivity of airway fluoroscopy for determining laryngotracheal pathology was 18%. Specificity, positive predictive value and negative predictive value were 83%, 67% and 35%, respectively. Although each fluoroscopic exposure was optimized for pediatric patients, the median cumulative exposure to ionizing radiation was 19 mR (range 10–34 mR). ConclusionsAirway fluoroscopy yields metrics that are overall poor to be considered a valid and accurate universal radiologic diagnostic test in infants evaluated for laryngotracheal pathology. The cumulative exposure to ionizing radiation from use of a fluoroscope cannot be justified by the sensitivity of the test and may not conform to ALARA standards for imaging in this population.

Exploration of Molecular Network Variations in Different Subtypes of Human Non-functional Pituitary Adenomas.

Exploration of Molecular Network Variations in Different Subtypes of Human Non-functional Pituitary Adenomas.

Comparitivee Analysis of Cognition and Language in Autism, Mental Retardation and Co-Morbid Autism with Mental Retardation

Aims: The aim of this study was to correlate the IQ of the language impaired children in autism and mental retardation with their level of development and to understand its effect on the potential to learn language in high functioning autistic, low functioning autistic and mentally retarded children. Method: The study sample was divided into three groups, notably mental retardation without autism, autism without mental retardation and autism with mental retardation. A total number of 15 subjects were recruited from each group. To maintain the consistency of the IQ mild to moderate range of MR was selected. The tools used for assessment were the Communication DEALL (Development Eclectic Approach to Language Learning), 3D LAT (Language Acquisition Test), and CARS (The Childhood Autism Ratting Scale). CARS was used to determine the diagnosis and severity of autism. The expressive and receptive language including the cognition of the children, assessed by 3D LAT was compared with the scores given by the Communication DEALL in the same areas. Result: The high functioning autistic showed a more significant improvement in all the parameters than the low functioning autistic and the mentally retarded children Conclusion: I.Q. may act as a limiting factor for low functioning autistic but high functioning autistic have problems due to specific deficits like language and perceptions. Emphasis should be placed on training of Activities of Daily Living (ADL).

Detection of mu opioid receptor (MOPR) and its glycosylation in rat and mouse brains by western blot with anti-μC, an affinity-purified polyclonal anti-MOPR antibody.

Our experience demonstrates that it is difficult to identify MOPR in rat and mouse brains by western blot, in part due to low abundance of the receptor and a wide relative molecular mass (Mr) range of the receptor associated with its heterogeneous glycosylation states. Here, we describe generation and purification of anti-μC (a rabbit polyclonal anti-MOPR antibody), characterization of its specificity in immunoblotting of HA-tagged MOPR expressed in a cell line, and ultimately, unequivocal detection of the MOPR in brain tissues by western blot with multiple rigorous controls. In particular, using brain tissues from MOPR knockout (K/O) mice as the negative controls allowed unambiguous identification of the MOPR band, since the anti-MOPR antibody, even after affinity purification, recognizes nonspecific protein bands. The MOPR was resolved as a faint, broad, and diffuse band with a wide Mr range of 58-84 kDa depending on brain regions and species. Upon deglycosylation to remove N-linked glycans by PNGase F (but not Endo H), the MOPR became a dense and sharp band with Mr of ~43 kDa, close to the theoretical Mr of its deduced amino acid sequences. Thus, MOPRs in rodent brains are differentially glycosylated by complex type of N-linked glycans in brain region- and species-specific manners. Furthermore, we characterized the MOPR in an A112G/N38D-MOPR knockin mouse model that possesses the equivalent substitution of the A118G/N40D SNP in the human MOPR gene. The substitution removes one of the four and five N-linked consensus glycosylation sites of the mouse and human MOPR, respectively. We demonstrated that the Mr of the MOPR in A112G mouse brains was lower than that in wild-type mouse brains, and that the difference was due to lower degrees of N-linked glycosylation.

N-glycan analysis of mannose/glucose specific lectin from Dolichos lablab seeds

An affinity purified mannose/glucose specific lectin from the seeds of Dolichos lablab (Indian bean/lablab bean) resolves into five subunits upon SDS-PAGE in the range of Mr 12–20kDa. Partial de novo sequencing of subunits resulted in 88% and 73% sequence coverage for α and β subunits of the cDNA derived FRIL (Flt3 receptor interacting lectin) sequence, respectively and suggested that four bands correspond to the α-subunits while the band of lowest molecular mass is designated as β. It was proposed in an earlier study on FRIL that the difference in molecular mass of α-subunits is due to differences in C-terminal processing and differential N-glycosylation i.e. numbers of N-glycans present (Colucci et al., 1999). Thus, differential N-glycosylation of the purified mannose/glucose specific lectin was unravelled by in-gel trypsin/chymotrypsin digestion of the α-subunits followed by desalting and ZIC-HILIC enrichment of N-glycopeptides. Subsequently, analyses by nano electrospray ionisation quadrupole time of flight mass spectrometry and low-energy collision-induced dissociation experiments revealed the presence of a typical paucimannose type N-glycan (Man2(Xyl)GlcNAc2(Fuc)) in α subunits 2–4.

The excluded volume effect induced by poly(ethylene glycol) modulates the motility of actin filaments interacting with myosin

To examine the motility of actomyosin complexes in the presence of high concentrations of polymers, we investigated the effect of poly(ethylene glycol) on the sliding velocities of actin filaments and regulated thin filaments on myosin molecules in the presence of ATP. Increased concentrations and relative molecular masses of poly(ethylene glycol) decreased the sliding velocities of actin and regulated thin filaments. The decreased ratio of velocity in regulated thin filaments at - log[Ca(2+) ] of 4 was higher than that of actin filaments. Furthermore, in the absence of Ca(2+) , regulated thin filaments were moderately motile in the presence of poly(ethylene glycol). The excluded volume change (∆V), defined as the change in water volume surrounding actomyosin during the interactions, was estimated by determining the relationship between osmotic pressure exerted by poly(ethylene glycol) and the decreased ratio of the velocities in the presence and absence of poly(ethylene glycol). The ∆V increased up to 3.7 × 10(5) Å(3) as the Mr range of poly(ethylene glycol) was increased up to 20,000. Moreover, the ∆V for regulated thin filaments was approximately two-fold higher than that of actin filaments. This finding suggests that differences in the conformation of filaments according to whether troponin-tropomyosin complexes lie on actin filaments alter the ∆V during interactions of actomyosin complexes and influence motility.

Analysis of protein species differentiation among mycobacterial low-Mr-secreted proteins by narrow pH range Immobiline gel 2-DE-MALDI-MS

Secreted proteins of bacteria are preferentially capable of interacting with host cells and are therefore of special biological and medical interest. Narrow pH range 2-DE and MALDI-TOFTOF-MS combine high-resolution protein separation with highly sensitive identification of proteins. Secreted proteins of Mycobacterium tuberculosis were separated at the protein species level, distinguishing different protein species of one protein. We focused on the pI range 4.0-4.7 and the Mr range 6-20kDa of the 2-DE pattern. Out of 128 analyzed spots, 121 were identified resulting in 33 different proteins with 277 different protein species, accumulating in a mean of 8.4 protein species per protein. Overrepresentation was found for the protein classes "virulence, detoxification, adaption", "information pathways", "cell wall and cell processes" and "intermediary metabolism and respiration". Thus far, 15 protein species of the ESX-1 family are characterized with 100% sequence coverage. More automated 2-DE procedures and more sensitive identification techniques are required for complete characterization of all of the protein species even in highly enriched samples, such as culture filtrates. Only then the functional level of proteomics will be achieved and potential biomarkers can be postulated at the molecular level. Proteomics is dominated by bottom-up approaches largely ignoring protein speciation. A prerequisite to reach the protein species level is to obtain 100% sequence coverage, which is a major challenge in proteomics. Here we show complete sequence information with a 2-DE-MS approach for 15 protein species. Acetylation of the N-terminus of ESAT-6 inhibits interaction with CFP-10, with direct consequences for pathogen-host interaction. This article is part of a Special Issue entitled: Trends in Microbial Proteomics.

SP0093 Role of imaging in osteoarthritis: Biomarker, prognostic factor, outcome measure in RCT?

A biomarker of structural integrity seems essential in understanding the natural and post-therapy progression of osteoarthritis (OA), perhaps even more so at a time when a new wave of pharmacological...

Large-scale Identification of Endogenous Secretory Peptides Using Electron Transfer Dissociation Mass Spectrometry

Mass spectrometry-based unbiased analysis of the full complement of secretory peptides is expected to facilitate the identification of unknown biologically active peptides. However, tandem MS sequencing of endogenous peptides in their native form has proven difficult because they show size heterogeneity and contain multiple internal basic residues, the characteristics not found in peptide fragments produced by in vitro digestion. Endogenous peptides remain largely unexplored by electron transfer dissociation (ETD), despite its widespread use in bottom-up proteomics. We used ETD, in comparison to collision induced dissociation (CID), to identify endogenous peptides derived from secretory granules of a human endocrine cell line. For mass accuracy, both MS and tandem MS were analyzed on an Orbitrap. CID and ETD, performed in different LC-MS runs, resulted in the identification of 795 and 569 unique peptides (ranging from 1000 to 15000 Da), respectively, with an overlap of 397. Peptides larger than 3000 Da accounted for 54% in CID and 46% in ETD identifications. Although numerically outperformed by CID, ETD provided more extensive fragmentation, leading to the identification of peptides that are not reached by CID. This advantage was demonstrated in identifying a new antimicrobial peptide from neurosecretory protein VGF (non-acronymic), VGF[554-577]-NH2, or in differentiating nearly isobaric peptides (mass difference less than 2 ppm) that arise from alternatively spliced exons of the gastrin-releasing peptide gene. CID and ETD complemented each other to add to our knowledge of the proteolytic processing sites of proteins implicated in the regulated secretory pathway. An advantage of the use of both fragmentation methods was also noted in localization of phosphorylation sites. These findings point to the utility of ETD mass spectrometry in the global study of endogenous peptides, or peptidomics.

CATALOG AND STATISTICAL STUDY OF X-RAY SELECTED BL LACERTAE OBJECTS

This paper presents a catalog of 312 X-ray selected BL Lacerate objects (XBLs), optically identified through the end of 2011. It contains the names from different surveys, equatorial coordinates, redshifts, multifrequency flux values, and luminosities for each source. In addition, the different characteristics of XBLs are statistically investigated (redshift, radio/optical/X-ray luminosities, central black hole (BH) mass, synchrotron peak frequency, broadband spectral indices, optical flux variability). Their values are collected through an extensive bibliographic and database search or calculated by us. The redshifts range from 0.031 to 0.702 with a maximum of the distribution at z = 0.223. The 1.4 GHz luminosities of XBLs log νL ν ~ 39-42 erg s–1 while optical V and X-ray 0.1-2.4 keV bands show log νL ν ~ 43-46 erg s–1. The XBL hosts are elliptical galaxies with effective radii r eff = 3.2625.40 kpc and ellipticities, = 0.040.52. Their R-band absolute magnitudes MR range from –21.11 mag to –24.86 mag with a mean value of –22.83 mag. The V – R indices of the hosts span from 0.61 to 1.52 and reveal a fourth-degree polynomial relationship with z that enables us to evaluate the redshifts of five sources whose V – R indices were determined from the observations but whose irredshifts values are either not found or not confirmed. The XBL nuclei show a wider range of 7.31 mag for MR with the highest luminosity corresponding to MR = –27.24 mag. The masses of central BHs are found in the interval log M BH = 7.39-9.30 solar masses (with distribution maximum at log M BH/M ☉ = 8.30). The synchrotron peak frequencies are spread over the range log νpeak = 14.56–19.18 Hz with a peak of the distribution at log νpeak = 16.60 Hz. The broadband radio-to-optical (α ro ), optical-to-X-ray (α ox ), and radio-to-X-ray (α rx ) spectral indices are distributed in the intervals (0.17,0.59), (0.56,1.48), and (0.41,0.75), respectively. In the optical energy range, the overall flux variability increases, on average, towards shorter wavelengths: Δm = 1.22, 1.50, and 1.82 mag through the R, V, B bands of Johnson-Cousins system, respectively. XBLs seem be optically less variable at the intranight timescales compared to the radio-selected BL Lacs (RBLs).

Applicability of 2-DE to assess differences in the protein profile between cold storage and not cold storage in nectarine fruits

Cold storage is being used to increase nectarine fruits' postharvest life. However, low temperatures lead to chilling injury and limit their commercial quality and value. In this study a proteomic approach was used to compare the protein profile between control and cold storage nectarine fruits. Protein extracted from tissue was resolved by two-dimensional gel electrophoresis (2-DE) in the 4-7 pH and 10-200 kDa Mr range. Around 350 spots were well determined, and 11 from 17 spots that showed significant differences were identified by peptide mass fingerprinting (PMF) using matrix-assisted laser desorption/ionization (MALDI)-time-of-flight (TOF)-mass spectrometry (MS). Four differentially expressed proteins were characterized as allergens and were further assessed at the transcription level using quantitative real time-RT-PCR (qRT-PCR).

Implications of CT imaging for postplan quality assessment in prostate brachytherapy

Postplan quality assurance using CT shows considerable interobserver contour variability. We examined CT postplans of four experienced brachytherapists for comparison with MR-determined prostate volumes. Seventy-five patients had CT and MR scans 1 month post-(125)I prostate brachytherapy. CT scans were contoured by the treating physician and dosimetry calculated. The prostate was contoured independently on MR by one observer with extensive MR experience, the scans were fused and dosimetric parameters compared. The mean prostate volume on CT was 38.3 cc (17.5-78.6 cc), on MR 33.3 cc (16.3-66.1 cc). On average, the volume on CT was 16.1% larger than on MR (range, 8% smaller to 64% larger). Craniocaudal discordance of the CT vs. MR prostate contours ranged from 4 mm cranial to 10 mm caudal to MR base and from 6 mm cranial to 14 mm caudal to MR apex. The CT prostate volume not only included an average of 90% of the MR prostate (range, 75-99%) but also included normal tissue (mean, 8.3 cc; range, 2.9-17.1 cc). The average difference between the calculated D(90) from CT contours vs. MR contours was 10.0 Gy (standard deviation, 8.8; range, -37.6 to +41.6 Gy). On average, only 90% of the MR-defined prostate is included in CT contours, while a volume of normal tissue is erroneously designated as prostate. Lack of awareness of this deficiency in planning and/or operative technique gives a false sense of appreciation of the true conformality, delays implementation of corrective measures, and risks unnecessary side effects.

Defunciones en niños a principios de los siglos XX y XXI en Boyacá-Colombia

Comparing and analysing crude death (CDR), child mortality (CMR) 5-year per thousand live births (LBR) and infant mortality rates (IMR) in the Boyacá department, Colombia, during the early 20th and 21st centuries. A descriptive epidemiological comparative historical approach was adopted. Two data sources were used for deaths: parish registers 1912-1927 and the Boyacá Epidemiological Bulletin 2007 population census for 1912-1918-1927 and 2005 (Colombian Statistics Bureau - DANE); data was stored and analysed in Mysql. Crude rates, age-specific and child mortality ratios and mortality were calculated by cause. 7,958 deaths were recorded from 1912-1927 compared to 5,813 in the 21st century. CDR dropped from 150 to 42 deaths per 10,000 inhabitants; the IMR became significantly reduced from 231 to 17 for every 1,000 LB. Although specific rates for children under 8 years of age could be compared to children under 5, there was variability in the range and construction of the specific MR and that defined by WHO-UNICEF. There was a higher reduction in mortality in the 21st century due to national and international policies for controlling preventable diseases and maternal death. The 1918-19 flu pandemic significantly affected mortality in all population groups; violent causes were prominent in infant mortality in the 21st century.