Ramp Contractions Research Articles

Characterizing Mechanical Changes in the Biceps Brachii Muscle in Mild Facioscapulohumeral Muscular Dystrophy Using Shear Wave Elastography.

There is no general consensus on evaluating disease progression in facioscapulohumeral muscular dystrophy (FSHD). Recently, shear wave elastography (SWE) has been proposed as a noninvasive diagnostic tool to assess muscle stiffness in vivo. Therefore, this study aimed to characterize biceps brachii (BB) muscle mechanics in mild-FSHD patients using SWE. Eight patients with mild FSHD, the BB were assessed using SWE, surface electromyography (sEMG), elbow moment measurements during rest, maximum voluntary contraction (MVC), and isometric ramp contractions at 25%, 50%, and 75% MVC across five elbow positions (60°, 90°, 120°, 150°, and 180° flexion). The mean absolute percentage deviation (MAPD) was analyzed as a measure of force control during ramp contractions. The shear elastic modulus of the BB in FSHD patients increased from flexed to extended elbow positions (e.g., p < 0.001 at 25% MVC) and with increasing contraction intensity (e.g., p < 0.001 at 60°). MAPD was highly variable, indicating significant deviation from target values during ramp contractions. SWE in mild FSHD is influenced by contraction level and joint angle, similar to findings of previous studies in healthy subjects. Moreover, altered force control could relate to the subjective muscle weakness reported by patients with dystrophies.

Central and peripheral neuromuscular fatigue following ramp and rapid maximal voluntary isometric contractions.

Maximal voluntary isometric contractions (MVICs) as a fatiguing modality have been widely studied, but little attention has been given to the influence of the rate of torque development. Given the established differences in motor command and neuromuscular activation between ramp and rapid MIVCs, it is likely performance fatigue differs as well as the underlying physiological mechanisms. To compare responses for rapid and maximal torque following ramp and rapid MVICs, and the corresponding neuromuscular and corticospinal alterations. On separate visits, twelve healthy males (22.8 ± 2.5years) performed fatiguing intermittent MVICs of the knee extensors with either 2s (RAMP) or explosive (RAPID) ramp-ups until a 50% reduction in peak torque was achieved. Before and after each condition, maximal and rapid torque measures were determined from an MVIC. Additionally, peripheral (twitch parameters) and central (voluntary activation) fatigue, as well as rapid muscle activation, and cortical-evoked twitch and electromyographic responses were recorded. Maximal and late-phase rapid torque measures (p ≤ 0.001; = 0.635-0.904) were reduced similarly, but early rapid torque capacity (0-50ms) (p = 0.003; d = 1.11 vs. p = 0.054; d = 0.62) and rapid muscle activation (p = 0.008; d = 1.07 vs. p = 0.875; d = 0.06) decreased more after RAMP. Changes in peripheral fatigue, as indicated by singlet and doublet contractile parameters (p < 0.001 for all; = 0.752-0.859), and nerve-evoked voluntary activation (p < 0.001; 0.660) were similar between conditions. Corticospinal inhibition (via silent period) was only increased after RAPID (p = 0.007; d = 0.94 vs. p = 0.753; d = 0.09), whereas corticospinal voluntary activation and excitability were unchanged. Ramp, fatiguing MVICs impaired early rapid torque capacity more than rapid MVICs, and this was accompanied by decrements in rapid muscle activation. Responses for peripheral and central fatigue (nerve and cortical stimulation) were largely similar between conditions, except that rapid MVICs increased corticospinal inhibition.

Neuromodulatory Contribution to Muscle Force Production after Short-Term Unloading and Active Recovery.

Prior evidence has shown that neural factors contribute to the loss of muscle force after skeletal muscle disuse. However, little is known about the specific neural mechanisms altered by disuse. Persistent inward current (PIC) is an intrinsic property of motoneurons responsible for prolonging and amplifying the synaptic input, proportionally to the level of neuromodulation, thus influencing motoneuron discharge rate and force production. Here, we hypothesized that short-term unilateral lower limb suspension (ULLS) would reduce the neuromodulatory input associated with PIC, contributing to the reduction of force generation capacity. In addition, we tested whether physical exercise would restore the force generation capacity by reestablishing the initial level of neuromodulatory input. In 12 young adults, we assessed maximal voluntary contraction pre- and post-10 d of ULLS and after 21 d of active recovery (AR) based on resistance exercise. PIC was estimated from high-density surface electromyograms of the vastus lateralis muscle as the delta frequency (Δ F ) of paired motor units calculated during isometric ramped contractions. The values of Δ F were reduced after 10 d of ULLS (-33%, P < 0.001), but were fully reestablished after the AR (+29.4%, P < 0.001). The changes in estimated PIC values were correlated ( r = 0.63, P = 0.004) with the reduction in maximal voluntary contraction after ULLS (-29%, P = 0.002) and its recovery after the AR (+28.5%, P = 0.003). Our findings suggest that PIC estimates are reduced by muscle disuse and may contribute to the loss of force production and its recovery with exercise. Overall, this is the first study demonstrating that, in addition to peripheral neuromuscular changes, central neuromodulation is a major contributor to the loss of force generation capacity after disuse, and can be recovered after resistance exercise.

Effect of Static Stretching on Tendon Hysteresis and Efficiency During Repetitive Jumping.

Sasajima, S and Kubo, K. Effect of static stretching on tendon hysteresis and efficiency during repetitive jumping. J Strength Cond Res 38(6): 1041-1047, 2024-To date, no studies have experimentally shown a relationship between tendon hysteresis and exercise efficiency. However, previous studies showed that tendon hysteresis decreased immediately after static stretching. The purposes of this study were to (a) investigate the change in tendon hysteresis during the recovery period after static stretching and (b) determine whether exercise efficiency is enhanced because of the decline of tendon hysteresis after static stretching. For stretching (1 minute × 4 sets) and control conditions, tendon hysteresis was measured during ramp (i.e., lower strain rate of tendon) and ballistic (i.e., higher strain rate of tendon) contractions before, immediately, 15, 30, 45, and 60 minutes after interventions. In addition, electromyograms of the plantar flexor muscles (medial gastrocnemius [MG], lateral gastrocnemius [LG], and soleus muscles [SOL]) and oxygen consumption (V̇O 2 ) were measured during 10 minutes of submaximal repetitive jumping after both interventions. Tendon hysteresis (during ramp and ballistic contractions) reduced by static stretching persisted for up to 60 minutes (effect of time p < 0.001). During repetitive jumping, no differences in electromyograms of the plantar flexor muscles (effect of condition p = 0.786 for MG, p = 0.124 for LG, p = 0.682 for SOL) or V̇O 2 (effect of condition p = 0.534) were found between stretching and control conditions. These results suggest that the reduction in tendon hysteresis because of static stretching continues until 60 minutes after the end of stretching, and static stretching does not change the efficiency (evaluated by electromyograms of the plantar flexor muscles and V̇O 2 ) during submaximal repetitive jumping.

Control of motor output during steady submaximal contractions is modulated by contraction history.

The purpose of the study was to investigate the influence of contraction history on force steadiness and the associated EMG activity during submaximal isometric contractions performed with the dorsiflexor muscles. The key feature of the protocol was a triangular ramp contraction performed in the middle of a steady contraction at a lower target force. The target force during the ramp contraction was 20% MVC greater than that during the steady contraction. Thirty-seven healthy individuals (21 men and 16 women) performed the submaximal tasks with the ankle dorsiflexors. Electromyography (EMG) signals were recorded from tibialis anterior with a pair of surface electrodes. The coefficient of variation for force was significantly greater during the second steady contraction compared with the first one at each of the seven target forces (p<0.015; d=0.38-0.92). Although the average applied force during the steady contractions before and after the triangular contraction was the same (p=0.563), the mean EMG amplitude for the steady contractions performed after the triangular contraction was significantly greater at each of the seven target forces (p<0.0001; d=0.44-0.68). Also, there were significant differences in mean EMG frequency between the steady contractions performed before and after the triangular contraction (p<0.01; d=0.13-0.82), except at 10 and 20% MVC force. The greater force fluctuations during a steady submaximal contraction after an intervening triangular contraction indicate a change in the discharge characteristics of the involved motor units.

Common synaptic inputs and persistent inward currents of vastus lateralis motor units are reduced in older male adults

Although muscle atrophy may partially account for age-related strength decline, it is further influenced by alterations of neural input to muscle. Persistent inward currents (PIC) and the level of common synaptic inputs to motoneurons influence neuromuscular function. However, these have not yet been described in the aged human quadriceps. High-density surface electromyography (HDsEMG) signals were collected from the vastus lateralis of 15 young (mean ± SD, 23 ± 5 y) and 15 older (67 ± 9 y) men during submaximal sustained and 20-s ramped contractions. HDsEMG signals were decomposed to identify individual motor unit discharges, from which PIC amplitude and intramuscular coherence were estimated. Older participants produced significantly lower knee extensor torque (p < 0.001) and poorer force tracking ability (p < 0.001) than young. Older participants also had lower PIC amplitude (p = 0.001) and coherence estimates in the alpha frequency band (p < 0.001) during ramp contractions when compared to young. Persistent inward currents and common synaptic inputs are lower in the vastus lateralis of older males when compared to young. These data highlight altered neural input to the clinically and functionally important quadriceps, further underpinning age-related loss of function which may occur independently of the loss of muscle mass.

Reduced oxygen desaturation in the vastus lateralis of chronic stroke survivors during graded muscle contractions

ABSTRACT Background Few studies have examined changes in skeletal muscle physiology post-stroke. This study examined changes in tissue oxygen saturation (StO2) of the vastus lateralis (VL) muscle of stroke survivors and age-matched control participants during maximal and submaximal isometric contractions of the knee extensor muscles. Objectives We hypothesized that tissue oxygen desaturation (ΔStO2) during knee extensor muscle contractions would be less in the VL in the paretic vs. the non-paretic and control legs. Methods Ten chronic stroke survivors (>6 months post-stroke) with lower extremity muscle weakness and 10 age-matched controls completed this prospective cohort study. Maximum voluntary contractions (MVCs) of the knee extensor muscles were assessed with a Biodex dynamometer and StO2 of the VL was measured using near-infrared spectroscopy. Results In the paretic leg of the stroke survivors little change in StO2 of the VL was observed during an MVC (ΔStO2 = -1.7 ± 1.8%) compared to the non-paretic (ΔStO2 = -5.1 ± 6.1%; p < 0.05) and control legs (ΔStO2 = -14.4 ± 8.8%; p < 0.05 vs. paretic and non-paretic leg). These differences remained when normalizing for strength differences between the legs. Compared to controls, both the paretic and non-paretic VL showed pronounced reductions in ΔStO2 during ramp and hold contractions equal to 20%, 40%, or 60% of the MVC (p < 0.05 vs. controls at all load levels). Conclusions These results indicate that oxygen desaturation in response to isometric muscle contractions is impaired in both the paretic and non-paretic leg muscle of stroke survivors compared to age-matched controls, and these differences are independent of differences in muscle strength.

Effects of muscular endurance and tendon extensibility on endurance of joint stiffness

ABSTRACT In some sporting events (e.g., long-distance running), the ability to maintain joint stiffness is considered an essential physical ability. However, the determinants of joint stiffness endurance remain unclear. This study aimed to examine the effects of muscular endurance and tendon extensibility on joint stiffness endurance. Thirteen males performed the fatigue task (5 sets of 50 hopping). Ankle joint stiffness during drop jump was measured before and after fatigue task. The maximum number of repetitions at 30% of one repetition maximum for plantar flexion was measured as muscular endurance. Maximal elongation of the Achilles tendon was measured during ramp (with a low strain rate of tendon) and ballistic (with a high strain rate of tendon) contractions as tendon extensibility. Joint stiffness significantly decreased by 7.5% after the fatigue task (p = 0.033). The maximum number of repetitions at 30% of 1RM (79.6 ± 48.7 repetitions) was not significantly correlated with the relative change in joint stiffness (r = 0.283, p = 0.348). The maximal elongation of the Achilles tendon measured during ramp and ballistic contractions were not significantly associated with the relative change in joint stiffness (r = 0.326, p = 0.277 for ramp contraction; r = 0.438, p = 0.135 for ballistic contraction). These results suggest that muscular endurance and tendon extensibility were unrelated to joint stiffness endurance.

A Wireless 2-Channel Layered EMG/NIRS Sensor System for Local Muscular Activity Evaluation.

A wireless 2-channel layered sensor system that enables electromyography (EMG) and near-infrared spectroscopy (NIRS) measurements at two local positions was developed. The layered sensor consists of a thin silver electrode and a photosensor consisting of a photoemitting diode (LED) or photodiode (PD). The EMG and NIRS signals were simultaneously measured using a pair of electrodes and photosensors for the LED and PD, respectively. Two local muscular activities are presented in detail using layered sensors. In the experiments, EMG and NIRS signals were measured for isometric constant and ramp contractions at each forearm using layered sensors. The results showed that local muscle activity analysis is possible using simultaneous EMG and NIRS signals at each local position.

Relationship between Chronic Obstructive Pulmonary Disease Severity and Surface Electromyography Parameters during Fatigue Caused by Knee Extensor Contractions

Objective: Skeletal muscle dysfunction, which is characterized by decreased muscle strength and endurance, contributes to dyspnea during exercise in patients with chronic obstructive pulmonary disease (COPD), regardless of pulmonary function. This study analyzed skeletal muscle function in patients with COPD through surface electromyography (sEMG) evaluations and signal processing and attempted to determine whether sEMG parameters for muscle fatigue reflect the course of the disease. Methods: In 24 patients with COPD, maximal voluntary isometric contraction and ramp contraction were performed during isometric knee extension, and the sEMG activity of the rectus femoris muscle was measured. The patients were divided into three groups according to their modified Medical Research Council (mMRC) grade and Global Initiative for Chronic Obstructive Lung Disease (GOLD) stage. Patients with mMRC grades 0 and 1 were classified into group A, grade 2 into group B, and grades 3 and 4 into group C. Patients with GOLD stage 1 were classified into group I, stage 2 into group II, and stages 3 and 4 into group III. sEMG parameters were compared between groups using one-way analysis of variance. Results: There were significant differences in the minimum median frequency and fatigue index between the mMRC groups. However, the sEMG parameters did not differ significantly among the GOLD groups. Conclusion: Muscle fatigue differs according to the severity of subjective dyspnea in COPD. sEMG evaluations and signal processing can play supplementary roles in evaluating muscle endurance and predicting functional capacity in COPD.

Determinants of joint stiffness and jumping height during drop jump.

The present study aimed to examine the effects of muscle-tendon mechanical properties and electromyographic activity on joint stiffness and jumping height and to explore the determinants of joint stiffness and jumping height. Twenty-nine males performed unilateral drop jumps at three drop heights (10, 20, and 30 cm) using only the ankle joint on the sledge apparatus. Ankle joint stiffness, jumping height, and electromyographic activity of the plantar flexor muscles were measured during drop jumps. Active muscle stiffness of the medial gastrocnemius muscle was calculated according to changes in the estimated muscle force and fascicle length during fast stretching at five different angular velocities (100, 200, 300, 500, and 600 deg s-1 ) after submaximal isometric contractions. Tendon stiffness and elastic energy were measured during ramp and ballistic contractions. Active muscle stiffness was significantly correlated with joint stiffness, except for a few conditions. Tendon stiffness measured during ramp and ballistic contractions was not significantly correlated with joint stiffness. The ratios of electromyographic activity before landing and during the eccentric phase to that during the concentric phase were significantly correlated with joint stiffness. In addition, jumping heights at 10 and 20 cm (except for 30 cm) drop heights were strongly associated with the tendon elastic energy, whereas no other measured variables showed significant correlations with jumping heights. These results suggested that (1) joint stiffness is determined by active muscle stiffness and electromyographic activity patterns during jumping, and (2) jumping height is determined by tendon elastic energy.

Can local vibration alter the contribution of persistent inward currents to human motoneuron firing?

The response of spinal motoneurons to synaptic input greatly depends on the activation of persistent inward currents (PICs), which in turn are enhanced by the neuromodulators serotonin and noradrenaline. Local vibration (LV) induces excitatory Ia input onto motoneurons and may alter neuromodulatory inputs. Therefore, we investigated whether LV influences the contribution of PICs to motoneuron firing. This was assessed in voluntary contractions with concurrent, ongoing LV, as well as after a bout of prolonged LV. High-density surface electromyograms (HD-EMG) of the tibialis anterior were recorded with a 64-electrode matrix. Twenty males performed isometric, triangular, dorsiflexion contractions to 20% and 50% of maximal torque at baseline, during LV of the tibialis anterior muscle, and after 30-min of LV. HD-EMG signals were decomposed, and motor units tracked across time points to estimate PICs through a paired motor unit analysis, which quantifies motor unit recruitment-derecruitment hysteresis (ΔF). During ongoing LV, ΔF was lower for both 20% and 50% ramps. Although significant changes in ΔF were not observed after prolonged LV, a differential effect across the motoneuron pool was observed. This study demonstrates that PICs can be non-pharmacologically modulated by LV. Given that LV leads to reflexive motor unit activation, it is postulated that lower PIC contribution to motoneuron firing during ongoing LV results from decreased neuromodulatory inputs associated with lower descending corticospinal drive. A differential effect in motoneurons of different recruitment thresholds after prolonged LV is provocative, challenging the interpretation of previous observations and motivating future investigations. KEY POINTS: Neuromodulatory inputs from the brainstem influence motoneuron intrinsic excitability through activation of persistent inward currents (PICs). PICs make motoneurons more responsive to excitatory input. We demonstrate that vibration applied on the muscle modulates the contribution of PICs to motoneuron firing, as observed through analysis of the firing of single motor units. The effects of PICs on motoneuron firing were lower when vibration was concurrently applied during voluntary ramp contractions, likely due to lower levels of neuromodulation. Additionally, prolonged exposure to vibration led to differential effects of lower- vs. higher-threshold motor units on PICs, with lower-threshold motor units tending to present an increased and higher-threshold motor units a decreased contribution of PICs to motoneuron firing. These results demonstrate that muscle vibration has the potential to influence the effects of neuromodulation on motoneuron firing. The potential of using vibration as a non-pharmacological neuromodulatory intervention should be further investigated.

Longitudinal development of muscle strength and relationship with motor unit activity and muscle morphological characteristics in youth athletes.

Neural and morphological adaptations determine gains of muscle strength. For youth athletes, the importance of morphological adaptation is typically highlighted based on the change in maturity status. However, the long-term development of neural components in youth athletes remains unclear. The present study investigated the longitudinal development of muscle strength, muscle thickness (MT), and motor unit firing activity of the knee extensor and their relationships in youth athletes. Seventy male youth soccer players (mean ± SD age = 16.3 ± 0.6years) performed neuromuscular, maximal voluntary isometric contraction (MVC), and submaximal ramp contraction (at 30 and 50% MVC) tests with knee extensors, two times with a 10-month measurement interval. High-density surface electromyography was recorded from the vastus lateralis and decomposed to identify each individual motor unit activity. MT was evaluated by the sum of the vastus lateralis and vastus intermedius thicknesses. Finally, sixty-four participants were employed to compare MVC and MT, and 26 participants were employed to analyze motor unit activity. MVC and MT were increased from pre to post (p < 0.05, 6.9 and 1.7% for MVC and MT, respectively). Y-intercept of the regression line between median firing rate vs. recruitment threshold was also increased (p < 0.05, 13.3%). Multiple regression analysis demonstrated that the gains of both MT and Y-intercept were explanatory variables for the gain of strength. These findings suggest that the neural adaptation could also make the important contribution to the strength gain for the youth athletes over a 10-month training period.

Investigating post-mild traumatic brain injury neuromuscular function and musculoskeletal injury risk: A protocol for a prospective, observational, case–controlled study in service members and active individuals

IntroductionMusculoskeletal injury (MSKI) risk is increased following mild traumatic brain injury (mTBI). Increased MSKI risk is present up to 2 years following post-mTBI return-to-duty/activity relative to both non-mTBI peers and...

Blockade of 5-HT2 receptors suppresses motor unit firing and estimates of persistent inward currents during voluntary muscle contraction in humans.

Serotonergic neuromodulation contributes to enhanced voluntary muscle activation. However, it is not known how the likely motoneurone receptor candidate (5-HT2 ) influences the firing rate and activation threshold of motor units (MUs) in humans. The purpose of this study was to determine whether 5-HT2 receptor activity contributes to human MU behaviour during voluntary ramped contractions of differing intensity. High-density surface EMG (HDsEMG) of the tibialis anterior was assessed during ramped isometric dorsiflexions at 10, 30, 50 and 70% of maximal voluntary contraction (MVC). MU characteristics were successfullyextracted from HDsEMG of 11 young adults (four female) pre- and post-ingestion of 8mg cyproheptadine or a placebo. Antagonism of 5-HT2 receptorscaused a reduction in MU discharge rate during steady-state muscle activation that was independent of the level of contraction intensity [P<0.001; estimated mean difference (∆)=1.06pulses/s], in addition to an increase in MU derecruitment threshold (P<0.013, ∆=1.23% MVC), without a change in force during MVC (P=0.652). A reduction in estimates of persistent inward current amplitude was observed at 10% MVC (P<0.001, ∆=0.99 Hz) and 30% MVC (P=0.003, ∆=0.75 Hz) that aligned with 5-HT changes in MU firing behaviour attributable to 5-HT2 antagonism. Overall, these findings indicate that 5-HT2 receptor activity has a role in regulating the discharge rate in populations of spinal motoneurones when performing voluntary contractions. This study provides evidence of a direct link between MU discharge properties, persistent inward current activity and 5-HT2 receptor activity in humans. KEY POINTS: Activation of 5-HT receptors on the soma and dendrites of motoneurones regulates their excitability. Previous work using chlorpromazine and cyproheptadine has demonstrated that the 5-HT2 receptor regulates motoneurone activity in humans with chronic spinal cord injury and non-injured control subjects. It is not known how the 5-HT2 receptor directly influences motor unit (MU) discharge and MU recruitment in larger populations of human motoneurones during voluntary contractions of differing intensity. Despite the absence of change in force during maximal voluntary dorsiflexions, 5-HT2 receptor antagonism caused a reduction in MU discharge rate during submaximal steady-state muscle contraction, in addition to an increase in MU derecruitment threshold, irrespective of the submaximal contraction intensity. Reductions in estimates of persistent inward currents after 5-HT2 receptor antagonism support the viewpoint that the 5-HT2 receptor plays a crucial role in regulating motor activity, whereby a persistent inward current-based mechanism is involved in regulating the excitability of human motoneurones.

A Wireless Multi-Layered EMG/MMG/NIRS Sensor for Muscular Activity Evaluation.

A wireless multi-layered sensor that allows electromyography (EMG), mechanomyography (MMG) and near-infrared spectroscopy (NIRS) measurements to be carried out simultaneously is presented. The multi-layered sensor comprises a thin silver electrode, transparent piezo-film and photosensor. EMG and MMG measurements are performed using the electrode and piezo-film, respectively. NIRS measurements are performed using the photosensor. Muscular activity is then analyzed in detail using the three types of data obtained. In experiments, the EMG, MMG and NIRS signals were measured for isometric ramp contraction at the forearm and cycling exercise of the lateral vastus muscle with stepped increments of the load using the layered sensor. The results showed that it was possible to perform simultaneous EMG, MMG and NIRS measurements at a local position using the proposed sensor. It is suggested that the proposed sensor has the potential to evaluate muscular activity during exercise, although the detection of the anaerobic threshold has not been clearly addressed.

A method for measuring muscle strength in restraining valgus joint angulation: Elbow varus muscle strength against valgus loading

Skeletal muscle works as a dynamic joint stabilizer, assisting the underlying ligaments in restricting joint angulation by actively resisting external loads. Despite its clinical importance, little is known about the muscle strength required to produce torque to help ligaments restrict joint angulation within the physiological range permitted by the joint structure. In this study, we introduce a method for measuring the strength of the elbow musculature in restraining valgus angulation and present the values obtained in 20 healthy young men. Each participant was fastened to a Biodex dynamometer, with the elbow joint flexed to 90° and the varus-valgus axis aligned to the dynamometer’s rotation axis. Maximal voluntary isometric ramp contraction of shoulder internal rotators was performed while the humeroulnar joint gap was monitored with an ultrasound apparatus. The largest torque recorded while the humeroulnar joint gap did not exceed a predetermined individualized threshold was considered to be the elbow varus strength of the participant. The elbow varus strength of the dynamic stabilizer was found to be 41 ± 12 Nm, which agreed with the value estimated by our musculoskeletal model. The inter-operator reliability test indicated excellent reliability (ICC (2,1) = 0.91). These findings suggest that the present method is valid for measuring the strength of the elbow musculature in restraining the valgus angulation. Measurements of this aspect of strength are expected to provide insights for understanding and preventing elbow injuries.

The effects of α-lactalbumin supplementation and handgrip contraction on soleus motoneuron excitability

We tested two strategies that hypothetically increase serotonin availability (α-lactalbumin consumption and a remote submaximal handgrip contraction) on estimates of persistent inward currents (PICs) amplitude of soleus muscle in healthy participants. With a randomised, double-blind, and cross-over design, 13 healthy participants performed triangular-shaped ramp contractions with their plantar flexors (20% of maximal torque), followed by a 30-s handgrip sustained contraction (40% of maximal force) and consecutive repeated triangular-shaped contractions. This was performed before and after the consumption of either 40g of α-lactalbumin, an isonitrogenous beverage (Zein) or an isocaloric beverage (Corn-starch). Soleus motor units discharge rates were analysed from high-density surface electromyography signals. PICs were estimated by calculating the delta frequency (ΔF) of motor unit train spikes using the paired motor unit technique. ΔF (0.19 pps; p = 0.001; d = 0.30) and peak discharge rate (0.20 pps; p < 0.001; d = 0.37) increased after the handgrip contraction, irrespective of the consumed supplement. No effects of α-lactalbumin were observed. Our results indicate that 40g of α-lactalbumin was unable to modify intrinsic motoneuron excitability. However, performing a submaximal handgrip contraction before the plantar flexion triangular contraction was capable of increasing ΔF and discharge rates on soleus motor units. These findings highlight the diffused effects of serotonergic input, its effects on motoneuron discharge behaviour, and suggest a cross-effector effect within human motoneurons.

Shear wave elastography characterizes passive and active mechanical properties of biceps brachii muscle in vivo

Mechanical characterization of individual muscles in their in vivo environment is not well studied. Shear wave elastography (SWE) as a non-invasive technique was shown to be promising in quantifying the local mechanical properties of skeletal muscles. This study aimed to investigate the mechanics of the biceps brachii muscle (BB) derived from SWE in relation to elbow joint position and contraction intensity during isometric contraction.14 healthy, young subjects participated in the study and five different joint positions (60°–180° elbow angle) were investigated. Shear elastic modulus and surface electromyography (sEMG) of the BB and elbow torque were measured simultaneously, both in passive (i.e., resting) and active states during slow, sub-maximal isometric ramp contractions up to 25%, 50%, and 75% of the maximum voluntary contraction.At passive state, the shear elastic modulus of the BB increased with increasing elbow angle (p < 0.001). Maximum elbow flexion torque was produced at 60° and it decreased with increasing elbow angle (p = 0.001). During sub-maximal contractions, both elbow angle (p < 0.001) and contraction intensity (p < 0.001) had significant effects on the shear elastic modulus but only contraction intensity (p < 0.001) affected sEMG amplitude of the BB. Although torque was decreased at extended elbow positions (150°, 180°), higher active shear elastic modulus of BB muscle was found compared to flexed positions (60°, 90°). Linear regression of the BB sEMG amplitude over elbow torque showed good agreement for all joint positions (R2 between 0.69 and 0.89) while the linear agreement between shear elastic modulus of BB and elbow torque differed between flexed (R2 = 0.70 at 60° and R2 = 0.79 at 90°) and extended positions (with the lowest R2 = 0.57 at 150°).We conclude that using SWE, we can detect length-dependent mechanical changes of BB both in passive and active states. More importantly, SWE can be used to characterize active muscle properties in vivo. The present findings have critical importance for developing muscle stiffness as a measure of individual muscle force to validate muscle models and using SWE in clinical diagnostics.

Motor unit firing patterns of lower leg muscles during isometric plantar flexion with flexed knee joint position

The ankle flexor and extensor muscles are essential for pedal movements associated with car driving. Neuromuscular activation of lower leg muscles is influenced by the posture during a given task, such as the flexed knee joint angle during car driving. This study aimed to investigate the influence of flexion of the knee joint on recruitment threshold-dependent motor unit activity in lower leg muscles during isometric contraction. Twenty healthy participants performed plantar flexor and dorsiflexor isometric ramp contractions at 30 % of the maximal voluntary contraction (MVC) with extended (0°) and flexed (130°) knee joint angles. High-density surface electromyograms were recorded from medial gastrocnemius (MG), soleus (SOL), and tibialis anterior (TA) muscles and decomposed to extract individual motor units. The torque-dependent change (Δpps /Δ%MVC) of the motor unit activity of MG (recruited at 15 %MVC) and SOL (recruited at 5 %MVC) muscles was higher with a flexed compared with an extended knee joint (p < 0.05). The torque-dependent change of TA MU did not different between the knee joint angles. The motor units within certain limited recruitment thresholds recruited to exert plantar flexion torque can be excited to compensate for the loss of MG muscle torque output with a flexed knee joint.