Radiofrequency Radiation Exposure Research Articles

Investigations of Cell Tower Antennas Parameters on Reduction of Radio Frequency Radiation Levels from Radio Base Stations

populated areas has raised public health concerns due to increased exposure to radio frequency (RF) radiation emissions. Exposure to high levels of RF radiation can have potential thermal and non-thermal biological effects. Optimizing the configuration of cell tower antenna parameters is crucial for mitigating these radiation levels. This study therefore aims at systematically investigating the influence of different cell tower antenna parameters on reducing the RF radiation levels from mobile base stations. Field measurements were conducted at two cell sites shared by multiple mobile operators. Electric field strengths were measured at multiple locations around the cell sites. The impacts of various antenna parameters were analyzed, including the deployment of GSM spectrum within LTE services, number of carrier frequencies, antenna down-tilt, and transmission power. The analysis revealed that deploying the GSM spectrum within LTE services can reduce the overall radiation levels. Additionally, decreasing the number of carrier frequencies, increasing antenna down-tilt, and lowering transmission power were found to contribute to significant reductions in measured radiation levels. However, optimizing these antenna parameters to mitigate radiation can also degrade key mobile network performance indicators, such as coverage and capacity. These findings provide valuable insights into effective strategies for balancing the need to minimize RF radiation exposure from mobile base stations while maintaining acceptable mobile network service quality. Careful optimization of cell tower antenna configurations is essential to address public health concerns without compromising network performance.

The histological and biochemical analysis of the effects of radiofrequency radiation on testis tissue of rats and the protective effect of melatonin.

Primarily due to wireless communication devices, especially mobile phones, there has been a steady rise in the intensity of nonionizing radiofrequency radiation (RFR). In recent years, increased human health problems raised concerns about whether there is a positive relationship between intense exposure to RFR and public health. The present study aims to investigate the effects of GSM-like RFR exposure on the male reproductive system and the impact of melatonin treatment (synergistic, antagonist, or additive). Thirty-six male Wistar Albino rats were used and separated into six groups: i. Control; ii. Sham; iii. RFR exposure; iv. Control-melatonin; v. Sham-melatonin; vi. Melatonin + RFR exposure. Animals were exposed to 2600 MHz RFR with electric (E) field levels of 21.74 V/m for 30 min per day, 5 days per week, for 4 weeks. All testicular tissue samples were evaluated under a light microscope for hematoxylin-eosin staining. Biochemical analyses were performed by measuring malondialdehyde, total nitric oxide, glutathione, and glutathione peroxidase levels. We evaluated the combined effects of prolonged RFR exposure and melatonin treatment on ROS-mediated structural changes in testicular tissues. Results showed that reactive intermediates (malondialdehyde and total nitric oxide) increased significantly with RFR exposure, while the protective effect of melatonin effectively reduced the radical levels of the tissues. Histological evaluation revealed a decrease in cell population and connective tissue elements under RFR exposure, accompanied by marked edema in the testicular tissues. The structural and functional effects of prolonged RFR exposure might be ROS-based. Moreover, these adverse effects might be compensated with externally treated supplements. There is a need for new extensive research.

Short and long-term 2100 MHz radiofrequency radiation causes endoplasmic reticulum stress in rat testis

Long-term radiofrequency radiation (RFR) exposure, which adversely affects organisms, deteriorates testicular functions. Misfolding or unfolding protein accumulation in the endoplasmic reticulum (ER) initiates an intracellular reaction known as ER stress (ERS), which activates the unfolded protein response (UPR) for proteostasis. Since both RFR exposure and ERS can cause male infertility, we hypothesized that RFR exposure causes ERS to adversely affect testicular functions in rats. To investigate role of ERS in mediating RFR effects on rat testis, we established five experimental groups in male rats: control, short-term 2100-megahertz (MHz) RFR (1-week), short-term sham (sham/1-week), long-term 2100-MHz RFR (10-week), and long-term sham (sham/10-week). ERS markers Grp78 and phosphorylated PERK (p-Perk) levels and ERS-related apoptosis markers Chop and caspase 12 were investigated by immunohistochemistry, immunoblotting, and quantitative real-time polymerase chain reaction (qPCR). Long-term RFR exposure increased Grp78, p-Perk, and Chop levels, while short-term RFR exposure elevated Chop and caspase 12 levels. Chop expression was not observed in spermatogonia and primary spermatocytes, which may protect spermatogonia and primary spermatocytes against RFR-induced ERS-mediated apoptosis, thereby allowing transmission of genetic material to next generations. While short and long-term RFR exposures trigger ERS and ERS-related apoptotic pathways, further functional analyses are needed to elucidate whether this RFR-induced apoptosis has long-term male infertility effects.

Rat brain and testicular tissue effects of radiofrequency radiation exposure: Histopathological, DNA damage of brain and qRT-PCR analysis

Rat brain and testicular tissue effects of radiofrequency radiation exposure: Histopathological, DNA damage of brain and qRT-PCR analysis

Radio frequency electromagnetic radiations interfere with the Leydig cell functions in-vitro.

A growing threat to male infertility has become a major concern for the human population due to the advent of modern technologies as a source of radiofrequency radiation (RFR). Since these technologies have become an integral part of our daily lives, thus, it becomes necessary to know the impression of such radiations on human health. In view of this, the current study aims to focus on the biological effects of radiofrequency electromagnetic radiations on mouse Leydig cell line (TM3) in a time-dependent manner. TM3 cells were exposed to RFR emitted from 4G cell phone and also exposed to a particular frequency of 1800 MHz and 2450 MHz from RFR exposure system. The cells were then evaluated for different parameters such as cell viability, cell proliferation, testosterone production, and ROS generation. A considerable reduction in the testosterone levels and proliferation rate of TM3 cells were observed at 120 min of exposure as compared to the control group in all exposure settings. Conversely, the intracellular ROS levels showed a significant rise at 60, 90 and 120 min of exposure in both mobile phone and 2450 MHz exposure groups. However, RFR treatment for different time durations (15, 30, 45, 60, 90, and 120 min) did not have significant effect on cell viability at any of the exposure condition (2450 MHz, 1800 MHz, and mobile phone radiation). Therefore, our findings concluded with the negative impact of radiofrequency electromagnetic radiations on Leydig cell's physiological functions, which could be a serious concern for male infertility. However, additional studies are required to determine the specific mechanism of RFR action as well as its long-term consequences.

Expression levels of tam receptors and ligands in the testes of rats exposed to short and middle-term 2100 MHz radiofrequency radiation.

With advances in technology, the emission of radiofrequency radiation (RFR) into the environment, particularly from mobile devices, has become a growing concern. Tyro 3, Axl, and Mer (TAM) receptors and their ligands are essential for spermatogenesis and testosterone production. RFR has been shown to induce testicular cell apoptosis by causing inflammation and disrupting homeostasis. This study aimed to investigate the role of TAM receptors and ligands in the maintenance of homeostasis and elimination of apoptotic cells in the testes (weeks), short-term sham exposure (sham/1 week), and middle-term sham exposure (sham/10 weeks). Testicular morphology was assessed using hematoxylin-eosin staining, while immunohistochemical staining was performed to assess expression levels of TAM receptors and ligands in the testes of all groups. The results showed that testicular morphology was normal in the control, sham/1 week, and sham/10 weeks groups. However, abnormal processes of spermatogenesis and seminiferous tubule morphology were observed in RFR exposure groups. Cleaved Caspase 3 immunoreactivity showed statistically significant difference in 1and 10 weeks exposure groups compared to control group. Moreover, there was no significant difference in the immunoreactivity of Tyro 3, Axl, Mer, Gas 6, and Pros 1 between groups. Moreover, Tyro 3 expression in Sertoli cells was statistically significantly increased in RFR exposure groups compared to the control. Taken together, the results suggest that RFR exposure negatively affects TAM signalling, preventing the clearance of apoptotic cells, and this process may lead to infection and inflammation. As a result, rat testicular morphology and function may be impaired.

Radiofrequency radiation reshapes tumor immune microenvironment into antitumor phenotype in pulmonary metastatic melanoma by inducing active transformation of tumor-infiltrating CD8+ T and NK cells

Immunosuppression by the tumor microenvironment is a pivotal factor contributing to tumor progression and immunotherapy resistance. Priming the tumor immune microenvironment (TIME) has emerged as a promising strategy for improving the efficacy of cancer immunotherapy. In this study we investigated the effects of noninvasive radiofrequency radiation (RFR) exposure on tumor progression and TIME phenotype, as well as the antitumor potential of PD-1 blockage in a model of pulmonary metastatic melanoma (PMM). Mouse model of PMM was established by tail vein injection of B16F10 cells. From day 3 after injection, the mice were exposed to RFR at an average specific absorption rate of 9.7 W/kg for 1 h per day for 14 days. After RFR exposure, lung tissues were harvested and RNAs were extracted for transcriptome sequencing; PMM-infiltrating immune cells were isolated for single-cell RNA-seq analysis. We showed that RFR exposure significantly impeded PMM progression accompanied by remodeled TIME of PMM via altering the proportion and transcription profile of tumor-infiltrating immune cells. RFR exposure increased the activation and cytotoxicity signatures of tumor-infiltrating CD8+ T cells, particularly in the early activation subset with upregulated genes associated with T cell cytotoxicity. The PD-1 checkpoint pathway was upregulated by RFR exposure in CD8+ T cells. RFR exposure also augmented NK cell subsets with increased cytotoxic characteristics in PMM. RFR exposure enhanced the effector function of tumor-infiltrating CD8+ T cells and NK cells, evidenced by increased expression of cytotoxic molecules. RFR-induced inhibition of PMM growth was mediated by RFR-activated CD8+ T cells and NK cells. We conclude that noninvasive RFR exposure induces antitumor remodeling of the TIME, leading to inhibition of tumor progression, which provides a promising novel strategy for TIME priming and potential combination with cancer immunotherapy.

Assessment of Radio-Frequency Radiation Exposure from Selected Mobile Base Stations in Yenagoa, Bayelsa State, Nigeria

The antennas from mobile base stations radiate non-ionizing radio frequency radiation into space. The human body can experience negative health effects from prolonged exposure to radiofrequency (RF) radiation. Because base station installations and telecommunication networks are growing, there is a growing need to address the health risks connected with exposure to telecommunication masts. Using the Electrosmog Meter Cornet Model, power density from many telecom masts owned by various network operators was measured in this investigation. At 10-meter intervals, the RF radiation in seven (7) settlements in Yenagoa town, located in the Yenagoa Local Government Area of Bayelsa State, was measured within radial distances of 100 meters. With a mean value of 4.0569mWm-2, the measured values vary from 0.650mWm-2 to 9.810mWm-2. Still, values evolved. Nevertheless, additional factors, such as adjacent electromagnetic sources' wave interference or reference base station interference, caused readings to fluctuate. It was discovered that the greatest power density level was 9.810 mWm-2, and it was measured 20 meters away from the base station at site MBS1. As per the International Commission on Non-ionizing Radiation Protection (ICNIRP) and other regulatory authorities, the standard limit of 4.5Wm-2 for the 900 MHz system is not exceeded by the radiation exposure level, as the findings show. This shows that the exposure levels in these areas are low enough to not seriously jeopardize the health of those living in the research area.

Stress-responsive MAPK signaling pathway with proliferation and apoptosis in the rat testis after 2100 MHz radiofrequency radiation exposure

Objective: Mobile phone technology has progressed quickly in recent years. Cell phones operate using radiofrequency radiation (RFR), and the complete biological impacts of RFR remain unidentified. Thus, we aimed to investigate the potential effects of 2100 MHz radiofrequency radiation exposure on the stress-responsive JNK/p38 MAPK pathway, apoptosis and proliferation in rat testis. Methods: RFR groups were created with 2100 MHz RFR exposure for acute (2 h/day for 1 week) and chronic (2 h/day for 10 weeks) periods. Sham groups were kept under identical conditions without RFR. The cell apoptosis and histopathological changes in testis were evaluated. Immunolocalization of PCNA, active caspase-3, Bcl-xL, p-JNK and p-p38 were analyzed by immunohistochemistry, the total protein expressions were identified by Western blot. Results: There were no differences between RFR and sham groups by means of histopathology and TUNEL analysis. Also, the expression levels and the immunolocalization patterns of PCNA, active caspase-3 and Bcl-xL proteins were not altered. p-JNK and p-p38 protein expressions were prominently elevated in acute and chronic RFR groups. Conclusion: In conclusion, 2100 MHz RFR exposure had no considerably deleterious consequences on cellular proliferation and apoptosis processes in rat testis. However, increased expression of stress-activated protein kinases, p-JNK and p-p38, suggests the involvement of the MAPK signaling pathway as a critical (may be detrimental) cellular response.

Genetic profiling of rat gliomas and cardiac schwannomas from life-time radiofrequency radiation exposure study using a targeted next-generation sequencing gene panel.

The cancer hazard associated with lifetime exposure to radiofrequency radiation (RFR) was examined in Sprague Dawley (SD) rats at the Ramazzini Institute (RI), Italy. There were increased incidences of gliomas and cardiac schwannomas. The translational relevance of these rare rat tumors for human disease is poorly understood. We examined the genetic alterations in RFR-derived rat tumors through molecular characterization of important cancer genes relevant for human gliomagenesis. A targeted next-generation sequencing (NGS) panel was designed for rats based on the top 23 orthologous human glioma-related genes. Single-nucleotide variants (SNVs) and small insertion and deletions (indels) were characterized in the rat gliomas and cardiac schwannomas. Translational relevance of these genetic alterations in rat tumors to human disease was determined through comparison with the Catalogue of Somatic Mutations in Cancer (COSMIC) database. These data suggest that rat gliomas resulting from life-time exposure to RFR histologically resemble low grade human gliomas but surprisingly no mutations were detected in rat gliomas that had homology to the human IDH1 p.R132 or IDH2 p.R172 suggesting that rat gliomas are primarily wild-type for IDH hotspot mutations implicated in human gliomas. The rat gliomas appear to share some genetic alterations with IDH1 wildtype human gliomas and rat cardiac schwannomas also harbor mutations in some of the queried cancer genes. These data demonstrate that targeted NGS panels based on tumor specific orthologous human cancer driver genes are an important tool to examine the translational relevance of rodent tumors resulting from chronic/life-time rodent bioassays.

Radio Frequency Radiation Exposure from Selected Mobile Transceiver Stations in Minna, Nigeria

Lately, there has been massive development in the use of mobile phones especially in developing countries. But electromagnetic radiation (EMR) emissions from various mobile transceiver stations (MTS) have raised debates on whether they are hazardous to human or not. In this study, we aim at presenting an assessment of human exposure to RF radiation from mobile transceiver stations in Minna. The power flux densities radiations (W/m2) on residential buildings around the MTS were measured using a handheld Extech RF EMF strength meter. The highest mean power flux density value recorded for Minna, was 69.17mW/m2 while the least value was 2.11mW/m2. The result shows that the measured mean power flux density from mobile transceiver stations in Minna, have been compared with standard limit set by International Commission on Non-Ionizing Radiation Protection (ICNIRP). The measured value is far below the threshold limit. Therefore, RF emission from MTS in this study area may pose no known health hazards to the general public within the chosen vicinity.

Protective effect of paricalcitol in rat testicular damage induced by subchronic 1800 MHz radiofrequency radiation

In the present study, the possible protective effects of paricalcitol (P) were investigated in testicular damage because of 1800 MHz radiofrequency radiation (RFR) exposure. Male Sprague Dawley rats 8–10 weeks old (n = 28) were randomly divided into four groups as control (C) (n = 7), RFR (n = 7, 1800 MHz RFR 1 h/day for 30 days), P (n = 7, 0.2 μg/kg paricalcitol, 3 times a week for 30 days), and RFR + P (n = 7, 1800 MHz RFR 1 h/day for 30 days +0.2 μg/kg paricalcitol, 3 times a week for 30 days). Testicular tissue was evaluated with histological and biochemical methods. No statistically significant differences were detected between the groups in seminiferous tubule diameters and germinal epithelial thicknesses. While ultrastructural changes were observed in the seminiferous tubule and Leydig cells in the RFR group, these changes were decreased in the RFR + P group. It was found that the Johnsen Score, Ki67, and p63 immunoreactivity scores (IRS), superoxide dismutase (SOD), and catalase (CAT) activities in the RFR + P group were statistically increased as compared to the RFR group and the malondialdehyde (MDA) levels were decreased statistically and significantly. These results show that paricalcitol administration may have an ameliorative effect on testicular damage occurring because of 1800 MHz RFR exposure.

A randomized comparison of retrograde left-sided versus anterograde right-sided ablation of the atrioventricular junction.

Catheter ablation of the atrioventricular node (AVN) is an effective treatment for patients with symptomatic atrial fibrillation. This study compares the success rate, procedure time, radiation time, and complication rates of retrograde left-sided (LSA) and anterograde right-sided (RSA) AVN ablation in a randomised controlled trial. Thirty-onepatients undergoing AVN ablation were randomized to either LSA (15 patients) or RSA (16 patients). Crossover occurred after six unsuccessful radiofrequency (RF) applications. The LSA cohort had a mean age of 77.00 ± 5.17 and the RSA cohort was 79.44 ± 6.08 (p = .0240). There were five crossovers from LSA to RSA and there was one crossover from RSA to LSA. There was no significant difference in ablation time between LSA and RSA (210.40 ± 179.77vs. 192.19 ± 130.29 seconds, p = .748). There was no significant difference in procedure time, fluoroscopy time, radiation dose, or number of RF applications between the two groups. There was 1 (6.67%) serious adverse event in the LSA group and 1 (6.25%) in the RSA group due to femoral hematomas requiring blood transfusion or intervention. There was no significant difference in patient-reported discomfort between LSA and RSA (16.43 ± 20.67 vs. 17.87 ± 28.08, p = .877). The study was stopped before full recruitment due to futility. Retrograde LSA of the AVN does not reduce RF applications, procedure time, or radiation exposure compared with conventional RSA and cannot be recommended as a first-line clinical approach.

3.5 GHz radiofrequency radiation may affect biomechanics of bone and muscle of diabetics

With the developments in wireless technologies, living beings are increasingly exposed to electromagnetic fields (EMFs). EMFs are known to affect bone metabolism and muscle tissue. However, their effects on bones and skeletal muscles are controversial, as some studies have reported positive effects while others have reported adverse effects. In this study, the effects of radiofrequency radiation (RFR) on bone biomechanics and skeletal muscle tissues were investigated in diabetic and healthy rats. Rats were exposed to 3.5 GHz RFR for 2 h per day for 30 days. Bone biomechanics measurements were taken to evaluate the effects of RFR on bone quality, flexibility and durability. The whole-body specific absorption rate (SAR) was found to be 37 mW/kg. The results showed that RFR exposure had adverse effects on bone biomechanics, including decreased elasticity coefficient and Young’s modulus, increased maximum displacement and decreased maximum force. However, oxidative stress parameters in diabetics were also altered by 3.5 GHz RFR to a greater extent than in healthy rats. In conclusion, 3.5 GHz RFR may have potential to alter bone quality and structural integrity including muscle oxidative stress parameters in rats. It should be emphasized that the observed changes were more obvious in diabetic rats. In addition, the changes observed in healthy and diabetic rats exposed to RFR showed a statistically significant difference according to the sham groups.

Cell Phone Radiation Exposure Limits and Engineering Solutions

In the 1990s, the Institute of Electrical and Electronics Engineers (IEEE) restricted its risk assessment for human exposure to radiofrequency radiation (RFR) in seven ways: (1) Inappropriate focus on heat, ignoring sub-thermal effects. (2) Reliance on exposure experiments performed over very short times. (3) Overlooking time/amplitude characteristics of RFR signals. (4) Ignoring carcinogenicity, hypersensitivity, and other health conditions connected with RFR. (5) Measuring cellphone Specific Absorption Rates (SAR) at arbitrary distances from the head. (6) Averaging SAR doses at volumetric/mass scales irrelevant to health. (7) Using unrealistic simulations for cell phone SAR estimations. Low-cost software and hardware modifications are proposed here for cellular phone RFR exposure mitigation: (1) inhibiting RFR emissions in contact with the body, (2) use of antenna patterns reducing the Percent of Power absorbed in the Head (PPHead) and body and increasing the Percent of Power Radiated for communications (PPR), and (3) automated protocol-based reductions of the number of RFR emissions, their duration, or integrated dose. These inexpensive measures do not fundamentally alter cell phone functions or communications quality. A health threat is scientifically documented at many levels and acknowledged by industries. Yet mitigation of RFR exposures to users does not appear as a priority with most cell phone manufacturers.

Genotoxic Risks to Male Reproductive Health from Radiofrequency Radiation.

During modern era, mobile phones, televisions, microwaves, radio, and wireless devices, etc., have become an integral part of our daily lifestyle. All these technologies employ radiofrequency (RF) waves and everyone is exposed to them, since they are widespread in the environment. The increasing risk of male infertility is a growing concern to the human population. Excessive and long-term exposure to non-ionizing radiation may cause genetic health effects on the male reproductive system which could be a primitive factor to induce cancer risk. With respect to the concerned aspect, many possible RFR induced genotoxic studies have been reported; however, reports are very contradictory and showed the possible effect on humans and animals. Thus, the present review is focusing on the genomic impact of the radiofrequency electromagnetic field (RF-EMF) underlying the male infertility issue. In this review, both in vitro and in vivo studies have been incorporated explaining the role of RFR on the male reproductive system. It includes RFR induced-DNA damage, micronuclei formation, chromosomal aberrations, SCE generation, etc. In addition, attention has also been paid to the ROS generation after radiofrequency radiation exposure showing a rise in oxidative stress, base adduct formation, sperm head DNA damage, or cross-linking problems between DNA & protein.

Effects of RF and ELF Radiation on Oxidative Stress of Brain Tissue and Plasma of Diabetic Rats

Objective: Exposure to Radio Frequency (RF) and Extremely Low Frequency (ELF) radiation is increasing steadily with the progress of technology and industrialization. The aim of this study was to investigate whether RF and ELF radiation are oxidative stress effects in the plasma and brain tissue of diabetic and non-diabetic rats. Methods: Experiment groups were designed as follows; C (control), S (sham), ELF (ELF radiation exposure), RF (RF radiation exposure), ELF+RF (ELF and RF radiation exposure), D-C (Diabetic Control), D-S (Diabetic Sham), D-ELF (Diabetic ELF), D-RF (Diabetic RF), D-ELF+RF (Diabetic ELF+RF). The experimental diabetes model was induced with a single dose of 65mg/kg streptozotocin (STZ). 2100 MHz RF and 50 Hz ELF radiation groups exposed for 1 month. Total nitric oxide (NOx), malondialdehyde (MDA) and total sulfhydryl groups (RSH) / glutathione (GSH) levels were measured in plasma and brain tissue. Results: RF + ELF radiation exposure caused an increase in NOx and MDA levels in plasma and brain tissue of diabetic and non-diabetic rats (p<0.05). Exposure to RF and RF + ELF radiation caused a decrease in plasma RSH / tissue GSH levels in non-diabetic rats (p<0.05). Conclusion: The most prominent effect was seen in the diabetic group with RF + ELF radiation exposure.

Can 900 MHz and 2100 MHz radiofrequency radiation exposure induce endoplasmic reticulum stress and apoptosis in rat thymus?

Objectives: Electrical appliances are source of radiofrequency radiation (RFR). The effects of RFR on the organism are not fully understood. Endoplasmic reticulum (ER) stress is appeared by the accumulation of misfolded proteins in ER lumen. The aim of this study was to investigate the effects of 900 and 2100 MHz RFR exposure on the ER stress pathway in rat thymus. Methods: Rats were divided into six groups: 1 week (w) and 10 w Sham rats were kept in plexiglass tubes for 2 hours/day without RFR, experiment groups were created as 1-w (acute) and 10 w (chronic) rats which exposed to 900 and 2100 MHz RFR for 2 h/day. There were 20 male Wistar rats in each group. Immunohistochemistry stainings were performed GRP78, CHOP, Cleaved (Clv.) Caspase 3 and Caspase 12. Results: Expressions of GRP78 and Clv. Caspase3 in RFR groups is significantly higher than sham groups (p < 0.001). In 900 MHZ-1 w rats, high levels of GRP78 expressions were at the cytoplasm of epithelial reticular cells. In other groups, GRP78 expressions were seen also at thymocytes. Expressions of CHOP in RFR rats were higher than sham rats (2100 MHz /Sham for 10 w; p < 0.001, 900 MHz /Sham for 10 w; p = 0.004, 900 MHz /Sham for 1 w; p = 0.003). Localization of CHOP expressions was at the nucleus membrane and cytoplasm. The expression of Caspase 12 in RFR rats was higher than sham rats (900 MHz /Sham for 1 w; p = 0.006, other groups; p < 0.001). Conclusions: This study demonstrates RFR exposure could increase levels of ER stress pathway proteins and could cause apoptosis.

Changes in the histopathology and in the proteins related to the MAPK pathway in the brains of rats exposed to pre and postnatal radiofrequency radiation over four generations

The development of new technologies and industry increases the number and variety of electromagnetic field (EMF) sources. Researcher are increasingly interested in the effects of EMF on brain health. The brain's function is largely dependent on electrical excitability, so it would be expected to be vulnerable to EMF. We therefore investigated the effects of brain development in the fetus, histopathological changes in female rats and the hippocampal level of MAPK proteins in male rats after exposed to pre and postnatal 2450 MHz continuous wave (CW) radiofrequency radiation (RFR) over four generations. Four groups; sham, irradiated female, irradiated male, irradiated male and female, with each consisting of four rats (one male and three females) were created. Rats in the exposure groups were whole-body exposed to 2450 MHz CW-RFR for 12 h/day during the experiment. Irradiation started one month before fertilization in the experimental group. On the 18th day of the gestational period, one pregnant rat from each group was decapitated under general anesthesia and the fetuses were taken. The remaining two pregnant rats completed the normal gestation period. When the offspring were two months old, four rats, one male and three female, were allocated for the second generation study. Next generation animals were also experienced the same processes as the first generation rats. This study were evaluated development of brain in fetuses and histopathological changes in brain of female rats using haematoxylin eosin staining, and the hippocampal level of MAPK proteins in brain of male rats by Western Bloting. We observed hemorrhagic areas, irregular cellular localization and vascular structures in the brain of fetal and adult female rat of exposed groups in the all generations. pERK, ptau, pJNK and pP38 were increased in the brain of adult male rat of exposed groups in the all generations (p < 0.005). Pre and postnatal 2450 MHz continuous wave radiofrequency radiation exposure may cause changes in the function of the MAPK pathway affecting cognitive processes such as learning and memory and may cause damage to both the fetus and adult brain tissue. Also, EMF may have potential to affect brain of future generations.

On radar and radio exposure and cancer in the military setting

IntroductionIn 2018, we reported a case series of 47 patients diagnosed with cancer following several years of exposure to high-intensity whole-body radiofrequency radiation (RFR) using the parameter of percentage frequency (PF). Consistent high and statistically significant PFs of hematolymphoid (HL) cancers were found in this group and in four previous reports on RFR-exposed groups in Belgium, Poland and Israel together with increased all-cancers rates. In this paper we report a new series of 46 young cancer patients who were exposed during military service to such radiation. Materials and methodsThe new group of patients comprises Israeli soldiers previously exposed to occupational RFR. The patients were self-selected to enroll in the research in cooperation with an NGO assisting patients with administrative counseling and legal and social services. The new group of patients was studied with respect to distribution (proportion) of cancer types using the method of PF. When possible, cancer risk ratios (RR) were estimated too. The results are compared to those of other occupational groups in three countries. ResultsMedian age at diagnosis was 23 years; duration of exposure was between 1 and 3 years and the latencies were short, median 4.6 years. The PF of HL cancers was 41.3%, 95% CI (27%–57%), versus 22.7% expected in non-exposed subjects matched for age and gender profiles, p = 0.003; 19 out of the 46 patients had HL cancers. The PF of Hodgkin lymphoma cancers was 21.7%, 95%CI (11%–36%), versus 11.6% expected, p = 0.033. For a subgroup of 6 patients, the number of soldiers in the units was known, and we were able estimate approximately the overall cancer risk ratio (RR) after 8 years as being 8.0 with 95% CI (2.9, 17), p < 0.002, with only 0.75 cases expected from the Cancer Registry data. In this subgroup, there were 3 HL cancer cases and 3 non-HL cases. Sarcoma PF was higher than expected, 7 out of the 46 patients were diagnosed with sarcoma, PF = 15.2%, 95%CI (6.3%–28.9%), p = 0.04 versus the expected PF of 7%. ConclusionThe HL PF was high and consistent with previous reports. Epidemiological studies on excess risk for HL and other cancers, brain tumors in cellphone users, and experimental studies on RFR and carcinogenicity strongly point to a cause-effect relationship. It is mandatory to reduce the RFR exposure of all personnel to that of the typical community levels, including the peak level of radar pulses. Radiation protection, safety instructions, cancer risk warnings and quantitative data on individual exposure together with regular medical monitoring must be instituted for all personnel exposed to such risks. The findings from our study add to the growing body of evidence underscoring the gross inadequacy of the International Commission on Non-Ionizing Radiation Protection (ICNIRP) thermal standards. Based on our findings and on the previous accumulated research, we endorse the recommendations to reclassify RFR exposure as a human carcinogen, International Agency for Research on Cancer (IARC) group 1.