Radioisotopes tend to play important roles in many fields, such as industry, healthcare, agriculture, aerospace, etc. Radioisotope production is mainly through accelerators or research reactors, and high flux research reactor is one of the most effective approaches for radioisotope production. The physical basis of preparing radioisotope relies on nuclear reactions occurring in the reactor core, which includes fission, (n,γ), (n,α), and (n,p) reaction, etc. Among them, fission and (n,γ) reaction are most important in the nuclear reactor. For example, the 99Mo could be generated by uranium fission and extracting from the fission products, or through the radiative capture reaction from enriched 98Mo. As for the fission method, the irradiation target is gradually transitioning from high enriched uranium (HEU) target to low enriched uranium (LEU) target due to the requirement of non-proliferation. In this paper, studies on the impacts of different fission targets on radioisotope productions are conducted. Moreover, an optimized study on the radiative capture method is performed to improve the production efficiency. It is concluded that it is advantageous to use radiative capture method to generate radioisotopes in high flux reactor, which helps to improve the specific activity with environmental friendliness.