ABSTRACT We present a statistical, photometric, and spectral energy distribution (SED) analysis to characterize the blue straggler stars (BSSs) populations in the Galactic old open cluster Berkeley 39. Berkeley 39 is a 6.16 Gyr old open cluster located at a distance of 3.99 Kpc.Gaia DR3 astrometry data have been used to estimate the membership probabilities using ensemble-based unsupervised machine learning techniques. We identified 21 BSS candidates on the colour–magnitude diagram, with 19 of them being detected in the Swift/UVOT UVW2 filter. We analysed the radial surface density profile and examined the cluster dynamical states and mass segregation effect. The SEDs of 19 BSSs are constructed using multiwavelength data covering UV to IR wavelengths. A single-component SED is fitted successfully for 14 BSS candidates. We discovered hot companions in five BSS candidates. These hot companions have temperatures of approximately 14 000 to 23 000 K, radii ranging from 0.04 to 0.13 R$_{\odot }$, and luminosities ranging from 0.16 to 2.91 L$_{\odot }$. Among these, three are most likely extremely low-mass white dwarfs (WDs) with masses around 0.17 to 0.18 M$_{\odot }$, and two are low-mass WDs with masses around 0.18 to 0.39 M$_{\odot }$. This confirms that they are post-mass transfer (Case A or Case B) systems. We also investigated the variable characteristics of BSSs by analysing their light curves using data from TESS. Our analysis confirms that two BSSs identified as eclipsing binaries in Gaia DR3 are indeed eclipsing binaries. Additionally, one of the two eclipsing binary BSSs shows evidence of having hot companions, as indicated by the multiwavelength SEDs.