Quarantine Surveillance Research Articles

Dose-responses of seven species of cerambycid beetles to synthesized pheromones in field experiments.

Adult male cerambycid beetles of the subfamilies Cerambycinae and Lamiinae emit aggregation-sex pheromones that attract both sexes, and these chemicals can be utilized for quarantine surveillance for related exotic species which produce the same or similar pheromones. Here, we assess how attraction of 7 cerambycid species to pheromone-baited traps was influenced by the release rates of synthesized pheromones from polyethylene sachet emitters. Compounds tested included racemic 3-hydroxyhexan-2-one, the (R)-enantiomer of which is the sole or major pheromone component of numerous cerambycine species, and 2 compounds that are pheromone components of many lamiine species: (E)-6,10-dimethyl-5,9-undecadien-2-yl acetate (fuscumol acetate) and 6-methylhept-5-en-2-ol (sulcatol). We confirmed release rates of these compounds could be manipulated by varying the doses loaded into emitters. Various doses and concentrations, ranging from very dilute to the neat compounds, were then tested in field trials. Several species were most strongly attracted to the highest dose of a tested compound, one species to an intermediate dose, while another species was equally attracted regardless of dose. Our results demonstrate the importance of characterizing dose-response relationships for cerambycid species targeted by pheromone-based trapping.

An artificial positive control for routine detection of rose rosette virus and Phyllocoptes fructiphilus that fit most primers for PCR, LAMP and RPA based assays

AbstractRose (Rosa spp.) is a very important ornamental shrub cultivated worldwide and of value for the pharmaceutical industry. The plant is broadly susceptible to pathogens, including viruses. Rose rosette virus (RRV; virus species Emaravirus rosae) causes multiple symptoms typically rosettes, ultimately leading to death. The virus transmission is by grafting and a wind‐dispersed eriophyoid mite, Phyllocoptes fructiphilus, which survives in winter‐dormant plants. Due to extensive globalization RRV is a threat for the European rose, landscape, nursery and tourism industries. The most common and reliable method used for RRV detection is RT‐PCR. Positive control is indispensable for PCR reliability and can be difficult to obtain for emerging or highly contagious pathogens and are subject to BSL‐2 quarantine. A synthetic artificial positive control (APC) using custom DNA inserts of sense and anti‐sense primers was designed de novo and inserted in a circular plasmid vector to create a positive control for use with most RRV reported primers and eriophyoid mites. This study describes a functional demonstration and development of a rapid, consistent, adaptable and cost‐effective alternative to infected true‐tissue positive control for detection of RRV. The inserted RRV primers are for end point and quantitative RT‐PCR, reverse transcription loop‐mediated isothermal amplification (RT‐LAMP), recombinase‐polymerase amplification (RPA), broad detection of emaravirus and the eriophyoid mite vector Phyllocoptes fructiphilus. The APC‐RRV and RRV infected rose (leaf tissue) were tested side to side. Results demonstrated APC‐RRV is a safe, cloneable and reliable approach subjected to quality control with application in quarantine surveillance and routine diagnostics of RRV.

Species‐specific primers for rapid detection of Monochamus saltuarius, an effective vector of Bursaphelenchus xylophilus in China

AbstractMonochamus saltuarius (Coleoptera: Cerambycidae) is a native pest common in pine forests of Northeast China and an effective dispersal vector for the invasive species Bursaphelenchus xylophilus, a cause of pine wilt disease. However, the lack of a rapid and accurate method for the identification of M. saltuarius limits surveillance and control efforts. In this study, we developed species‐specific primers, MsF/MsR, targeting cytochrome C oxidase subunit I (COI) for the rapid PCR‐based identification of M. saltuarius. Analyses of M. saltuarius sampled at different developmental stages and from five geographical origins supported the high specificity of the assay. Furthermore, M. saltuarius ‐specific COI primers amplified M. saltuarius DNA only with a lower limit of detection of 20 pg/μl, demonstrating the high sensitivity. The newly developed PCR‐based assay provides a rapid, simple, accurate and reproducible method for the determination of M. saltuarius regardless of condition, life stage and populations. The detection process could generally be completed within 2–3 hr and is therefore feasible for quarantine surveillance. The proposed approach may improve the early detection and continuous monitoring of M. saltuarius populations and thereby facilitate the control of B. xylophilus spread.

COVID-19 genome surveillance at international airport quarantine stations in Japan.

A coronavirus disease (COVID-19) genome surveillance has been conducted at four international airports in Japan, revealing a potential imported COVID-19 risk from multiple countries. The quarantine surveillance based on genome sequencing can enhance sequencing efforts worldwide, as returning travelers may serve as excellent sentinels for the global pandemic.

SURVEILLANCE INFRASTRUCTURES IN AND FOR CRISES: A COMPARATIVE ANALYSIS OF CHINA AND SOUTH KOREA’S DEVELOPMENT OF QUARANTINE SURVEILLANCE MOBILE APPLICATIONS DURING COVID-19

This article examines China and South Korea’s health surveillance infrastructures that are being developed and deployed during COVID19. To control the outbreak and maintain the state, the Chinese government implemented the "Alipay Health Code” in collaboration with technology giants like Alibaba, while South Korea launched a “self-quarantine safety protection app” to enforce home isolation to suspected carriers and monitor their health statuses. By comparatively analyzing these quarantine surveillance mobile applications that the Chinese and South Korean governments are utilizing in pandemic control, we investigate how these two different governmental regimes - one authoritarian and the other democratic - construct and propagate what their state-of-the-art surveillance technologies can offer to the public in moments of emergency. Through a mixture of walk-through method and situational analysis, this article aims to unpack the processes in which these technologies become developed and examine the politics around their deployment. More broadly, we argue that analyzing them offers new opportunities to investigate the relationship between state surveillance and personal privacy in the context of a national crisis. As surveillance tactics that were deemed oppressive and undemocratic in ordinary times get easily normalized in crisis situations, these moments allow us to reveal the precarious and flexible nature of surveillance and privacy while destabilizing the West-oriented, dichotomic understanding of these concepts. This article tackles this question by observing the relationships among relevant actors – the state officials, industry professionals, and general users – and various contestations/negotiations involved in the processes of designing and deploying these quarantine surveillance apps.

Molecular Diagnostic Assay for Rapid Detection of Flag Smut Fungus (Urocystis agropyri) in Wheat Plants and Field Soil.

Flag smut incited by Urocystis agropyri has the potential to cause substantial reduction in yield and quality of wheat production. An early and precise diagnosis is a key component in the successful management of flag smut of wheat. Therefore, a simple molecular assay for the rapid detection of U. agropyri was developed for the first time. To detect U. agropyri, species specific primers were developed by comparing the partial sequences of internal transcribed spacer (ITS) DNA region of U. agropyri with related and unrelated phytopathogenic fungi. The clear amplicons of 503 and 548 bp were obtained with the two sets of designed primers (UA-17F/UA-519R and UA-15F/UA-562R) from the genomic DNA of 50 geographic distinct isolates of U. agropyri. However, no amplicon was obtained from the DNA of other 21 related and unrelated phytopathogenic fungi which showed the specificity of the primers for the U. agropyri. PCR reaction was also set up to confirm the presence of U. agropyri spores in six different wheat varieties along with eleven distinct regional soil samples as template DNA. The presence of U. agropyri in all the soil samples collected from an infected field and plant tissue of diseased plants collected at two different stages (20 and 40 days post sowing) and the absence in the soils and plants of healthy plots indicated 100% reliability for detection of U. agropyri. This simple and rapid test can be employed for the detection of U. agropyri from enormous wheat and soil samples in very short time with less man power. Thus, the reported molecular assay is very specific for U. agropyri and requires less time and man power over conventional diagnosis which is often confused by coinciding morphological features of closely related fungal pathogens, and therefore, it can be used for quarantine surveillance of flag smut.

Identification of a male-produced sex-aggregation pheromone for a highly invasive cerambycid beetle, Aromia bungii

The longhorned beetle Aromia bungii (Coleoptera: Cerambycidae) is a major pest of stone fruit trees in the genus Prunus, including cherries, apricots, and peaches. Its native range includes China, Korea, Mongolia, and eastern Russia, but it has recently invaded and become established in several countries in Europe, and Japan, and it has been intercepted in shipments coming into North America and Australia. Here, we report the identification of its male-produced aggregation pheromone as the novel compound (E)-2-cis-6,7-epoxynonenal. In field trials in its native range in China, and in recently invaded areas of Japan, the pheromone attracted both sexes of the beetle. Thus, the pheromone should find immediate use in worldwide quarantine surveillance efforts to detect the beetle in incoming shipments. The pheromone will also be a crucial tool in ongoing efforts to eradicate the beetle from regions of the world that it has already invaded.

Influence of Trap Height and Bait Type on Abundance and Species Diversity of Cerambycid Beetles Captured in Forests of East-Central Illinois.

We assessed how height of panel traps above the forest floor, and the type of trap bait used, influenced the abundance and diversity of cerambycid beetles caught in forested areas of east-central Illinois. Panel traps were suspended from branches of hardwood trees at three heights above the ground: understory (∼1.5 m), lower canopy (∼6 m), and midcanopy (∼12 m). Traps were baited with either a multispecies blend of synthesized cerambycid pheromones or a fermenting bait mixture. Traps captured a total of 848 beetles of 50 species in the cerambycid subfamilies Cerambycinae, Lamiinae, Lepturinae, and Parandrinae, and one species in the closely related family Disteniidae. The species caught in highest numbers was the cerambycine Anelaphus pumilus (Newman), represented by 349 specimens. The 17 most abundant species (mean ± 1 SD: 45 ± 80 specimens per species) included 12 cerambycine and five lamiine species. Of these most abundant species, 13 (77%) were attracted to traps baited with the pheromone blend. Only the cerambycine Eburia quadrigeminata (Say) was attracted by the fermenting bait. Three species were captured primarily in understory traps, and another five species primarily in midcanopy traps. Variation among cerambycid species in their vertical distribution in forests accounted for similar overall abundances and species richness across trap height treatments. These findings suggest that trapping surveys of native communities of cerambycids, and quarantine surveillance for newly introduced exotic species, would be optimized by including a variety of trap baits and distributing traps across vertical strata of forests.

Pest behaviour insights from quarantine surveillance of male Queensland fruit fly, Bactrocera tryoni (Froggatt) (Diptera: Tephritidae)

Fruit flies (Diptera: Tephritidae) are a major problem for Australia's $6.9 billion horticultural industry. Bactrocera tryoni (Froggatt) is the most significant fruit fly pest in the eastern states of Australia. Catches of male B. tryoni from cuelure-baited Lynfield surveillance traps in towns bordering the Fruit Fly Exclusion Zone in New South Wales, Australia, from November to May in 2001/2002–2007/2008 were analysed with respect to year, town, property type (urban/rural), and tree species/crop varieties in which the trap was placed (tree type). Average catches of all traps were highest in 2007/2008 and lowest in 2003/2004, and showed an overall positive correlation with rainfall (P < 0.05). More B. tryoni males were caught in Wagga Wagga and Lockhart than in other towns. There were no significant differences in catches of B. tryoni between urban and rurally located traps. With respect to tree type, traps placed in pomefruit trees caught significantly more flies than those in citrus, stonefruit, or nonfruit trees, with no significant differences among the latter three tree groups. Within the pomefruit group, traps in apples (Malus domestica Borkh.) and pears (Pyrus spp.) caught significantly more flies than those in cotoneasters (Cotoneaster spp). Within the citrus group, traps in orange trees (Citrus × sinensis (L.) Osbeck) caught significantly more flies than those in mandarin trees (Citrus reticulata Blanco). No significant differences were detected in catches between any members of the stonefruit group. Within the nonfruit group, traps placed in kurrajongs (Brachychiton populneum (Schott & Endl.) R. Br.) caught significantly more flies than those placed on other tree species in the group. This study demonstrates that although surveillance programs conducted to establish and/or maintain area freedom from specific pests may not provide balanced datasets, careful analysis of the results of such programs can provide valuable insights into the behaviour of the species in question.

Gorgon Project: environmental acoustic recognition sensor (EARS)

The Asian house gecko (Hemidactylus frenatus) presents a significant risk to the unique environment of Barrow Island, the location of the Chevron-operated Gorgon Project. The island’s climate would suit the establishment of this non-indigenous species, which could displace the five known native gecko species. Originating in Asia, this pest has spread to almost 9 new locations by hitchhiking on international vessels and cargo. To help detect possible gecko activity on the Gorgon Project, a unique device has been developed to specifically detect the sound of the gecko call. EARS can detect the sound of a gecko’s call from up to 2 m away. Connected to a wireless network, it sends an alert when a call is registered detailing time, location, and a sound file to aid detection. Placed on cargo and vessels travelling to Barrow, it is also deployed in Australian supply bases, international fabrication yards, and marine offloading facilities on the island. EARS provide Gorgon Quarantine with a constant presence and are particularly useful for detecting nocturnal species. It complements Gorgon’s existing quarantine surveillance programs and is one of many measures to ensure Barrow Island remains free from non-indigenous species. The Gorgon Project is operated by an Australian subsidiary of Chevron and is a joint venture of the Australian subsidiaries of Chevron (47.3%), ExxonMobil (25%), Shell (25%), Osaka Gas (1.25%), Tokyo Gas (1%) and Chubu Electric Power (0.417%).

One-step reverse transcription loop-mediated isothermal amplification for the rapid detection of cucumber green mottle mosaic virus

Cucumber green mottle mosaic virus (CGMMV) has caused serious damage to Cucurbitaceae crops worldwide. The virus is considered one of the most serious Cucurbitaceae quarantine causes in many countries. In this study, a highly efficient and practical one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) was developed for the detection of CGMMV. The total RNA or crude RNA extracted from watermelon plants or seeds could be detected easily by this RT-LAMP assay. The RT-LAMP assay was conducted in isothermal (63°C) conditions within 1h. The amplified products of CGMMV could be detected as ladder-like bands using agarose gel electrophoresis or visualized in-tube under UV light with the addition of a fluorescent dye. The RT-LAMP amplification was specific to CGMMV, as no cross-reaction was observed with other viruses. The RT-LAMP assay was 100-fold more sensitive than that of reverse-transcription polymerase chain reaction (RT-PCR). This is the first report of the application of the RT-LAMP assay to detect CGMMV. The sensitive, specific and rapid RT-LAMP assay developed in this study can be applied widely in laboratories, the field and quarantine surveillance of CGMMV.

Design of the quarantine surveillance for non-indigenous species of invertebrates on Barrow Island

– The Ministerial conditions for regulatory approval for the Gorgon gas project on Barrow Island included a quarantine surveillance program having detection power of 0.8 for non-indigenous species of terrestrial invertebrates, vertebrates and plants.

Development of a Bio-PCR Protocol for the Detection of Xanthomonas arboricola pv. pruni.

A real-time SYBR Green I assay was developed and evaluated as a biological and enzymatic polymerase chain reaction (Bio-PCR) protocol for the detection of Xanthomonas arboricola pv. pruni. Suppression subtractive hybridization was used to generate a X. arboricola pv. pruni-specific subtracted DNA library, using X. arboricola pv. corylina as the driver strain. Primer pair 29F/R, designed from cloned sequence, showed no homology to GenBank sequences and amplified a 344-bp product in all X. arboricola pv. pruni isolates. Compared with other published X. arboricola pv. pruni primers, this primer pair was shown to be the only one capable of differentiating X. arboricola pv. pruni from all other X. arboricola pathovars. A real-time assay was developed and shown to be capable of detecting less than 10 CFU and 0.1 pg of DNA. Epiphytic bacteria isolated from plum tissue was used to further evaluate the specificity of the assay. A Bio-PCR protocol, developed for field evaluation, confirmed X. arboricola pv. pruni isolation from asymptomatic and symptomatic plum tissue over a 9-week period between host flowering and the first appearance of leaf and fruit symptoms in an orchard. Dilution plating enabled X. arboricola pv. pruni numbers to be quantified, providing supportive evidence for the usefulness of the Bio-PCR protocol in plant pathology and quarantine surveillance.

A molecular protocol using quenched FRET probes for the quarantine surveillance of Tilletia indica, the causal agent of Karnal bunt of wheat

The current surveillance protocol for Karnal bunt of wheat in most countries, including the USA, European Union (EU), and Australia, involves the tentative identification of the spores based on morphology followed by a molecular analysis. Germination of spores is required for confirmation which incurs a delay of about two weeks, which is highly unsatisfactory in a quarantine situation. A two-step PCR protocol using FRET probes for the direct detection and identification of Tilletia indica from a very few number of spores (≤10) is presented. The protocol involves amplification of the ITS1 DNA segment in the highly repeated rDNA unit from any Tilletia species, followed by FRET analysis to detect and unequivocably distinguish T. indica and the closely related T. walkeri. This rapid, highly sensitive, fluorescent molecular tool is species-specific, and could supersede the conventional microscopic diagnosis used in a quarantine surveillance protocol for Karnal bunt which is often confounded by overlapping morphological characters of closely related species.

Insect damage in Eucalyptus: A review of plantations in Chile

AbstractChile has more than 330 000 ha of eucalypt plantations, predominantly in the eighth to the tenth region (approximately 34 to 41°S). Eucalyptus globulus ssp. globulus is the principal eucalypt planted, but Eucalyptus nitens, Eucalyptus camaldulensis, Eucalyptus delegatensis and Eucalyptus viminalis are also grown. There are four main insect pests that have been detected attacking these eucalypts in Chile: the defoliator Gonipterus scutellatus, the bark borers Phoracantha semipunctata and Phoracantha recurva and the native wood borer Chilecomadia valdiviana. More recently, Thyrinteina arnobia and Ctenarytaina eucalypti have been detected. Gonipterus scutellatus and P. recurva have been discovered in Chile within the last 2 years and it is hoped they may still be eliminated using a combination of biological control and chemical control of local populations. Phoracantha recurva and P. semipunctata are not considered a problem because attack only occurs in areas of water deficit, away from current eucalypt plantations. Chilecomadia valdiviana can damage plantations of E. nitens but rarely attacks other eucalypts.◊Chilecomadia valdiviana may cause future problems through further host shifts. Thyrinteina arnobia has only been detected during quarantine surveillance in the port of Valparaiso. Ctenarytaina eucalypti, recently detected in August 1999, had an initial limited distribution in the first region. However, since then, this insect has expanded its distribution south up to the tenth region. None of the insects recorded on eucalypts in Chile to date currently presents a threat to the eucalypt industry. It is also essential that additional resources are made available for their continued monitoring and control, particularly given that the 44% of plantations are held by small to medium property owners that would otherwise be unable to control a serious outbreak because of economic restrictions.

Public health response to a suspected case of Lassa fever

The aims of our response to this suspected case of Lassa fever were to identify people with high risk exposures to the case, and to institute quarantine surveillance for contacts where necessary.

COMPARISON OF THE EFFECTIVENESS OF TWO TRAPS FOR BACTROCERA TRYONI (FROGGATT) (DIPTERA: TEPHRITIDAE) AND IMPLICATIONS FOR QUARANTINE SURVEILLANCE SYSTEMS

The effectiveness of 2 traps for Queensland fruit fly, Bactrocera tryoni was compared for 1 year amongst relatively low populations at Applethorpe‐Stanthorpe in southeast Queensland. The Lynfield trap which is an inexpensive, non‐sticky, pot‐type trap was significantly more effective than the Jackson trap and was at least as reliable in the detection of fruit fly populations. Use of Lynfield traps in quarantine surveillance programs should allow for a greater trap density and a more sensitive detection system to be established without vastly increased operational costs.