Encapsulation Of Quantum Dots Research Articles

Exosome Heterogeneity Affects the Distal "Barrier-Crossing" Trafficking of Exosome Encapsulated Quantum Dots.

The biological activities of nanoparticles (NPs), which include endocytosis by macrophages and subsequent intracellular degradation and/or release, transfer to other cells, or translocation across tissue barriers, highly depend on their fate in living organisms. Yet, translocation across barriers, especially the distal "barrier-crossing" trafficking of NPs, is still unclear. The exosome (Exo) plays a crucial role in intercellular communication and biological barrier trafficking. Here, we report that ZnCdSe@ZnS quantum dots (QDs), as a representation of NPs in biomedical applications, could cross the blood-brain barrier and approach the mouse brain via active Exo encapsulation. By employing multiple techniques, we demonstrated that QDs were internalized by macrophages (J774A.1) and tumor cells (HeLa) and then released to the extracellular environment along with Exo. Exo encapsulation facilitates the distal barrier-crossing trafficking of QDs in vivo, while Exo biogenesis inhibitor GW4869 suppressed the QDs enriched in the brains of mice with a 4T1-Luc breast cancer xenograft. Interestingly, Exo heterogeneity affects the distal trafficking of enveloped QDs. Exo derived from tumorous HeLa cells, not macrophages, that were enriched in functional proteins with cell adhesion, cell migration, axon guidance, and cell motility, showed a better capacity for the remote trafficking of QDs. This study proposes Exo as a vehicle to deliver exogenous NPs to translocate across the distal barrier and provides further information for biomedical application and the risk assessment of NPs.

Luminescent Dynamics of Perovskite Quantum Dots Encapsulated in Metal–Organic Frameworks

The encapsulation of perovskite quantum dots (PQDs) in metal–organic frameworks (MOF) has emerged as a promising strategy to improve their environmental stability and tune their optical property. Although energy or charge transfer processes between PQDs and the MOF matrix have already been reported, the triplet-energy transfer (TET) process from MOF to PQD, which is useful for applications such as photon upconversion and photoredox catalysis, still remains unexplored. Herein, we encapsulated CH3NH3Pb(Br/I)3 PQDs (MAPb(Br/I)3) into a zinc isophthalate MOF (denoted as Y346) to construct a solid hybrid composite (PQDs@Y346). Compared with traditional colloid PQDs, the photostability of PQDs in PQDs@Y346 is enhanced by about hundreds of times due to the protection of Y346 skeleton, accompanied with a prominent photoinduced self-healing behavior. Furthermore, a slow but efficient (∼57.8%) Y346-to-PQDs TET process is observed in PQDs@Y346 composites, leading to a long-lived (∼3 ms) delayed emission from PQDs. Our findings suggest PQDs@Y346 composites hold great promise for future long-term optoelectronic and photochemical applications.

Re-Examination of the Polymer Encapsulation of Quantum Dots for Biological Applications

The application of semiconductor core–shell quantum dots in biology requires the dispersion of these nanoparticles in water. A widely used phase transfer strategy is their encapsulation with amphiphilic polymers, a method that is believed to preserve the original ligand shell of quantum dots. In this contribution, we test this belief for the first time using a common encapsulation protocol based on poly(styrene-co-maleic anhydride) and ethanolamine and demonstrate that the original quantum dot ligand shell is in fact altered during the polymer encapsulation. In particular, our results show that primary amines, which are typically added to increase the hydrophilicity of the encapsulating polymer, displace the original ligands as a necessary step in the phase transfer process. We demonstrate that the surface of quantum dots synthesized using phosphinic acid as a ligand is readily modified by ethanolamine and hence shows high phase transfer efficiency, whereas quantum dots synthesized using oleic acid ligands did not show reactivity toward the primary amine and failed to transfer in the same conditions. Instead, a thiol, mercaptoethanol, was employed to displace the original oleic acid ligands and promote efficient poly(styrene-co-maleic anhydride) encapsulation. This work also provides practical guidelines for how the quantum dot community can better design and implement phase transfer protocols for a given composition, in order to maximize the efficiency and durability of the resulting water-dispersible nanoparticles for biological applications.

Encapsulating Semiconductor Quantum Dots in Supramolecular Cages Enables Ultrafast Guest-Host Electron and Vibrational Energy Transfer.

In the field of supramolecular chemistry, host-guest systems have been extensively explored to encapsulate a wide range of substrates, owing to emerging functionalities in nanoconfined space that cannot be achieved in dilute solutions. However, host-guest chemistry is still limited to encapsulation of small guests. Herein, we construct a water-soluble metallo-supramolecular hexagonal prism with a large hydrophobic cavity by anchoring multiple polyethylene glycol chains onto the building blocks. Then, assembled prisms are able to encapsulate quantum dots (QDs) with diameters of less than 5.0 nm. Furthermore, we find that the supramolecular cage around each QD strongly modifies the photophysics of the QD by universally increasing the rates of QD relaxation processes via ultrafast electron and vibrational energy transfer. Taken together, these efforts expand the scope of substrates in host-guest systems and provide a new approach to tune the optical properties of QDs.

Multiple Interpenetrating Metal-Organic Frameworks with Channel-Size-Dependent Behavior for Selective Gossypol Detection and Perovskite Quantum Dot Encapsulation.

An interpenetrating structure endows metal-organic frameworks (MOFs) with many exciting applications, such as fluorescence detection and host-guest chemistry. Herein, two unique structure-interpenetrating In-MOFs (In-pdda-1 and In-pdda-2; H2pdda = 4,4'-(pyridine-2,5-diyl)dibenzoic acid) are constructed by different coordination configurations. The four-connected In3+ center shows a triangular-pyramidal configuration or a 2D rectangle, forming an unc topology for In-pdda-1 and a sql network for In-pdda-2, respectively. Two different interpenetrating modes created by linear rigid ligands and metal clusters are observed in the two MOFs (In-pdda-1, 8-fold interpenetrating mode; In-pdda-2, [2D + 2D] interpenetrating mode), which determine the channel-size-dependent properties in fluorescence applications. During the quantitative detection process of gossypol, the small rhombic channels divided by interpenetrating molecular planes of In-pdda-2 greatly limit the distance between the analyte and the probe, promoting electron transfer and energy transfer processes and thus resulting in a low detection limit (28.6 nM). In addition, the pore size effect of In-pdda-1 encouraged us to explore an in situ perovskite quantum dot encapsulation strategy to obtain a MAPbBr3@MOF material with tunable and stable luminescence properties. Both of the above channel-size-dependent fluorescence properties may provide inspiration for the structural design and specialized applications of MOF materials.

Poly(maleic anhydride) Shell Modified with Negatively and Positively Charged Groups to Control Zeta Potential and Hydrodynamic Size of Encapsulated Quantum Dots at Variable pH

AbstractWe prepared water‐soluble quantum dots (QDs) with tunable ξ‐potential varied from −38 to +20 mV and controlled sensitivity to pH level. Poly(maleic anhydride‐alt‐1‐tetradecene) (PMAT) was modified with bifunctional molecules containing sulfate, sulfonate, phosphate, phosphonate, and quaternary ammonium in different proportions. The sulfonate shell of QDs leads to a stable negative ξ‐potential in the biological pH range from 4.5 to 9.5, while modification with quaternary ammonium groups gives QDs with positive and pH‐independent charge. If nearly half of carboxyl groups of PMAT were modified with quaternary ammonium groups, ξ‐potential begins pH‐dependent with the isoelectric point at pH≈5.5. To obtain pH‐independent, neutrally charged QDs, the rest of carboxyl groups can be converted into sulfonate groups at a 1 : 1 ratio; sulfonate‐quaternary ammonium QDs show high colloidal stability in solutions with high ionic strength. In addition, encapsulated QDs show an unusual correlation between their hydrodynamic size and ζ‐potential: when ζ‐potential approaches zero, the hydrodynamic size markedly increases due to a decrease in the electrostatic repulsion component of the QDs diffusion coefficient.

Ultrasound-Triggered Liposomes Encapsulating Quantum Dots as Safe Fluorescent Markers for Colorectal Cancer.

Quantum dots (QDs) are a promising tool to detect and monitor tumors. However, their small size allows them to accumulate in large quantities inside the healthy cells (in addition to the tumor cells), which increases their toxicity. In this study, we synthesized stealth liposomes encapsulating hydrophilic graphene quantum dots and triggered their release with ultrasound with the goal of developing a safer and well-controlled modality to deliver fluorescent markers to tumors. Our results confirmed the successful encapsulation of the QDs inside the core of the liposomes and showed no effect on the size or stability of the prepared liposomes. Our results also showed that low-frequency ultrasound is an effective method to release QDs encapsulated inside the liposomes in a spatially and temporally controlled manner to ensure the effective delivery of QDs to tumors while reducing their systemic toxicity.

Encapsulation of Recrystallized Inorganic Perovskite Quantum Dots in Nonwoven Fluoropolymer Fibers

Since emerging Metal Halide Perovskites were attracting elevated attention from scientific and industrial communities. Although, we still yet to see commercial products based on perovskite materials a significant body of work has been already done. Here we present our own method of Metal Halide Perovskites integration into possible industrial applications. Our way of Perovskite Quantum Dots encapsulation in polymer fibers provides exceptional water and ambient stability as well as optimal photoluminescence.

Highly stable fluorescent probe based on mesoporous silica coated quantum dots for sensitive and selective detection of Cd2+

Cadmium ions have been of crucial concern due to the high biological toxicity and serious environmental risks. Various fluorescent Cd-sensitive probes have been reported with improved sensing properties, but still severely suffer from poor stability and insufficient selectivity. In this work, a stable fluorescent probe based on silica encapsulated quantum dots (QDs) have been developed for rapid, sensitive and selective detection of cadmium ion. To improve fluorescence stability, the strategy of mesoporous silica encapsulation was adopted, in which the mesoporous silica shell offers numerous channels for Cd2+. Further, the Forster Resonance Energy Transfer (FRET) system, where QDs@mSiO2 and rhodamine B (RB) are used as donors and acceptors respectively, has been constructed, in which the mesoporous silica shell also serves as spacers with tunable thickness for controlling the QD-RB distance. Under optimal conditions, the probes possess a sensitive fluorescence response with a detection limit of 1.25 μM. Visual detection can be realized by the obvious fluorescence changes of the FRET system. In addition, the FRET system shows promising sensing performances both in tap water samples and rice-washed water samples, confirming a great potential for practical application.

Drug Release Profiles of Mitomycin C Encapsulated Quantum Dots-Chitosan Nanocarrier System for the Possible Treatment of Non-Muscle Invasive Bladder Cancer.

Nanotechnology-based drug delivery systems are an emerging technology for the targeted delivery of chemotherapeutic agents in cancer therapy with low/no toxicity to the non-cancer cells. With that view, the present work reports the synthesis, characterization, and testing of Mn:ZnS quantum dots (QDs) conjugated chitosan (CS)-based nanocarrier system encapsulated with Mitomycin C (MMC) drug. This fabricated nanocarrier, MMC@CS-Mn:ZnS, has been tested thoroughly for the drug loading capacity, drug encapsulation efficiency, and release properties at a fixed wavelength (358 nm) using a UV–Vis spectrophotometer. Followed by the physicochemical characterization, the cumulative drug release profiling data of MMC@CS-Mn:ZnS nanocarrier (at pH of 6.5, 6.8, 7.2, and 7.5) were investigated to have the highest release of 56.48% at pH 6.8, followed by 50.22%, 30.88%, and 10.75% at pH 7.2, 6.5, and 7.5, respectively. Additionally, the drug release studies were fitted to five different pharmacokinetic models including pesudo-first-order, pseudo-second-order, Higuchi, Hixson–Crowell, and Korsmeyers–Peppas models. From the analysis, the cumulative MMC release suits the Higuchi model well, revealing the diffusion-controlled mechanism involving the correlation of cumulative drug release proportional to the function square root of time at equilibrium, with the correlation coefficient values (R2) of 0.9849, 0.9604, 0.9783, and 0.7989 for drug release at pH 6.5, 6.8, 7.2, and 7.5, respectively. Based on the overall results analysis, the formulated nanocarrier system of MMC synergistically envisages the efficient delivery of chemotherapeutic agents to the target cancerous sites, able to sustain it for a longer time, etc. Consequently, the developed nanocarrier system has the capacity to improve the drug loading efficacy in combating the reoccurrence and progression of cancer in non-muscle invasive bladder diseases.

Erasable Cs4PbBr6 quantum dots glass with switchable photoluminescence.

Encapsulating quantum dots (QDs) into a transparent glass matrix can not only increase their stability but also explore new luminescence properties. Herein, erasable Cs4PbBr6 QDs with switchable photoluminescence are successfully obtained by a heat-treatment method in a borosilicate glass matrix. First, QDs can self-crystalize in borosilicate glass without the heat treatment. Second, it is found that when the QDs glass is heat treated below Tg, QDs can be destroyed. Further, when the QDs glass is reheat treated above Tg, atom rearrangement can occur and QDs can reprecipitate in the glass matrix. Consequently, the switchable photoluminescence of QDs can be achieved. The special erasable QDs glass and switchable photoluminescence in this work can resolve the technological challenge of recovering the luminescence performance of QDs after they have been destroyed.

Fluorescent Nanohybrids from ZnS/CdSe Quantum Dots Functionalized with Triantennary, N-Hydroxy-p-(4-arylbutanamido)benzamide/Gallamide Dendrons That Act as Inhibitors of Histone Deacetylase for Lung Cancer.

N-Hydroxy-p-(4-arylbutanamido)benzamides (HABAB) belong to one class of histone deacetylase inhibitors (HDACi), which regulate deacetylation of lysine residue's amino group in histone, which results in chromatin constriction. In addition, transcriptional knockdown of the genetic loci possessing the suppressor genes of tumor occurs. A tripodal, HABAB-capped gallamide dendron possessing thiol anchoring unit was prepared by the click method. The resultant hydrophilic dendritic unit was easily attached on the outer layer of CdSe/ZnS (i.e., core/shell type) quantum dots by thiolate-Zn interaction, as supported via 1H NMR spectroscopic analysis of the conjugate with its original property of fluorescence. The resulting, water-miscible nanohybrid (nano-HTPB) which bore trivalent, peripheral HABABs as the HDACi was efficiently taken up by cells of lung cancer and transported into the nuclei of cells in 3 h, as confirmed by confocal microscopy analysis. The concentration levels of 50% inhibition (IC50) after 48 h incubation of the nano-HTPB for A549 and H1299 lung cancer cell lines were 14 and 18 nM, respectively, which were about 150-fold lower than those of the parent HTPB analogues. Nano-HTPB at 20 nM induced the knockdown of cell cycle at second growth/mitosis (i.e., G2/M) transition, which eventually led to apoptosis of lung cancer cells, demonstrating that the nano-HTPB was much more potent in inhibiting lung cancer cell growth in a synergistic manner than the parent HTPB analogues. In addition, the dendritic HABAB-capped nanohybrid, nano-HTPB, is more effective than the parent HTPB analogues both in vitro and in vivo. Furthermore, the nano-HTPB is more effective than the parent HTPB to increase the acetylation level of proteins related to histone and nonhistone like p53 and tubulin. Our results confirmed that covalent encapsulation of quantum dots with peripheral, triantennary HDACis represented a feasible strategy for synergistic drug delivery with enhanced biological effects.

Synthesis of luminescent biotinylated multivalent dendrimer encapsulated quantum dots and investigation on its physico-chemical interactions with biological receptor avidin

It is challenging to design and characterize molecular recognition pair consisting of both targeting and imaging property and it is also of particular interest to inquire into the limits of the affinity that can be achieved with designed bio-conjugate. This paper presents functionalization of luminescent quantum dots (QDs) employing multivalent dendrimer molecules conjugated with biotin as targeting moiety and photo-physical and thermodynamic behavior of this bio-conjugate in terms of highly affinic binding with avidin. It is demonstrated that fluorescence intensity of QDs remained almost unaffected when synthesized conjugate interacts with avidin and this is considered to be an important requisite for applications in imaging and sensing. The observed changes in average emission lifetime as well as values of lifetime components of avidin indicate the binding of biotin with tryptophan moiety of avidin. Calorimetric investigation reveals that binding of avidin with biotin-dendrimer conjugate is exothermic process accompanied with favorable enthalpy (ΔH = −21.6 kcal/mol) and unfavorable entropy (ΔS = −43.7 cal/mol/deg). These findings may help design of multiplexed assays where dendrimer conjugated QDs based probe molecules are immobilized via the avidin-biotin interactions which can have implications in development of immuno-histochemical techniques.

Hollow Microcapsules Through Layer-by-Layer Self-Assembly of Chitosan/Alginate on E. coli

Hollow microcapsules prepared via layer-by-layer (LbL) self-assembled polyelectrolytes are prevalent biomaterials in the synthesis of biocompatible delivery systems for drugs, imaging probes, and other macromolecules to control biodistribution and lower toxicity in vivo. The use of LbL self-assembly for the synthesis of these capsules provides several benefits including ease of fabrication, abundance in choice of substrates and coating material, as well as application-specific tunability. This study explores the development of hollow microcapsules by LbL assembly of chitosan and alginate onto live E. coli cells, and also provides a proof-of-concept of this capsule as a delivery platform through the encapsulation of quantum dots as a cargo. The study found that robust bilayers of chitosan/alginate can be formed onto the core substrate (E. coli) containing quantum dots as demonstrated with zeta potential analysis. Confocal microscopy was used to verify cell viability and the internalization of quantum dots into the cells as well as confirmation of the coating using fluorescein-labelled chitosan. Furthermore, transmission electron microscopy (TEM) was used to analyse cells coated with four-bilayers and showed a uniform coating morphology with a capsule thickness of 10-20 nm, which increased to 20- 50 nm for hollow capsules after cell lysis. Quantum dot retention in the capsules was demonstrated using fluorescence measurements. Overall, the study shows promising results of a novel fabrication method for hollow microcapsules that uses biocompatible polymers and mild core dissolution conditions using cell templates with applications in sustained release of therapeutics and imaging probes.

Fluorescence enhancement and encapsulation of quantum dots via a novel crosslinked vinyl-ether liquid crystals/polymer composite film

Fluorescence efficiency and stability of quantum dots (QDs) film, which are both key for optoelectronic device, are relatively difficult to be improved simultaneously. Herein, the facile synthesis of vinyl-ether terminated liquid crystals was reported. Based on the host vinyl-ether liquid crystals, the polymer dispersed vinyl-ether liquid crystals (PDVLC), polymer dispersed crosslinked vinyl-ether liquid crystals (PDCVLC) and fluorine-containing polymer dispersed crosslinked vinyl-ether liquid crystals (F-PDCVLC) QDs films were fabricated in succession by dual-step polymerization. As a result, the PDVLC film can realize fluorescence enhancement and tuning, whereas the PDCVLC film not only maintain fluorescence enhancement of PDVLC film but also improve the water-resistant performance. More importantly, the formation of crosslinked film makes it convenient to fabricate QDs film. Subsequently, the F-PDCVLC film can further improve the water-resistant properties by introducing fluorine-containing monomers to enhance the hydrophobic performance. This free-standing (F-) PDCVLC film may offer a new avenue to realize the optimization of QDs film performance.

On the mechanism of graphene quantum dot encapsulation by chitosan: A molecular dynamics study

The safe and effective encapsulation and scavenging of carbon nanomaterials is of great importance in bionanotechnology. Chitosan has been extensively studied as a scavenger due to its superior biocompatibility, biodegradability, and strong adsorption capacity. In this work, molecular dynamics simulation was employed to systematically investigate the interactions between chitosan and GQDs at the molecular level. Our simulation demonstrates that chitosan chains in a highly basic solution can rapidly aggregate due to intermolecular hydrophobic interaction and encapsulate GQDs more efficiently, while chitosan chains in a highly acidic solution are relatively rigid and aggregate with difficulty due to repulsive electrostatic interaction, leading to a lower GQD capture efficiency. Moreover, with an increase in pH from 5.9 to 7.1, the hydrophobic segment of a chitosan chain increases and encourages the transition of the chitosan chain from a dispersed state to a self-aggregated state; thus, GQDs can be better encapsulated in the network of aggregated chitosan clusters. These results suggest that the behavior and mechanism of GQD encapsulation is mainly affected by the GQD-chitosan interaction and chitosan self-aggregation.

Dendritic cells reprogrammed by CEA messenger RNA loaded multi-functional silica nanospheres for imaging-guided cancer immunotherapy

The application and understanding of dendritic cell (DC) based immune cancer therapy are largely hindered by insufficient or improper presentation of antigens and the inability to track the homing of reprogrammed DCs to draining lymph nodes in real-time. To tackle these challenges, multi-functional and hierarchically structured silica nanospheres are rationally designed and fabricated, which encapsulate quantum dots to permit near infrared deep tissue imaging and are loaded with carcinoembryonic antigen messenger RNA (CEAmRNA) to enable stable and abundant antigen expression in DCs. After being injected into animals and inducing an antigen-specific immune response, the homing process of reprogrammed labelled DCs from peripheral tissues to draining lymph nodes can be simultaneously and precisely tracked. Significant inhibition of tumor growth is achieved via strong antigen-specific immune responses including induced DC maturation, enhanced T cell proliferation and cytotoxic T lymphocyte (CTL)-mediated responses. Both in vitro and in vivo experiments demonstrate the high effectiveness of this new strategy of imaging-guided cancer immunotherapy by using reprogrammed DCs as immunotherapeutic and tracking agents.

C(RGDyk)-modified nanoparticles encapsulating quantum dots as a stable fluorescence probe for imaging-guided surgical resection of glioma under the auxiliary UTMD

Surgical resection remains the preferred approach for some patients with glioblastoma (GBM), and eradication of the residual tumour niche after surgical resection is very helpful for prolonging patient survival. However, complete surgical resection of invasive GBM is difficult because of its ambiguous boundary. Herein, a novel targeting material, c(RGDyk)-poloxamer-188, was synthesized by modifying carboxyl-terminated poloxamer-188 with a glioma-targeting cyclopeptide, c(RGDyk). Quantum dots (QDs) as fluorescent probe were encapsulated into the self-assembled c(RGDyk)-poloxamer-188 polymer nanoparticles (NPs) to construct glioma-targeted QDs-c(RGDyk)NP for imaging-guided surgical resection of GBM. QDs-c(RGDyk)NP exhibited a moderate hydrodynamic diameter of 212.4 nm, a negative zeta potential of –10.1 mV and good stability. QDs-c(RGDyk)NP exhibited significantly lower toxicity against PC12 and C6 cells and HUVECs than free QDs. Moreover, in vitro cellular uptake experiments demonstrated that QDs-c(RGDyk)NP specifically targeted C6 cells, making them display strong fluorescence. Combined with ultrasound-targeted microbubble destruction (UTMD), QDs-c(RGDyk)NP specifically accumulated in glioma tissue in orthotropic tumour rats after intravenous administration, evidenced by ex vivo NIR fluorescence imaging of bulk brain and glioma tissue sections. Furthermore, fluorescence imaging with QDs-c(RGDyk)NP guided accurate surgical resection of glioma. Finally, the safety of QDs-c(RGDyk)NP was verified using pathological HE staining. In conclusion, QDs-c(RGDyk)NP may be a potential imaging probe for imaging-guided surgery.

High stability of silica-wrapped CsPbBr3 perovskite quantum dots for light emitting application

Encapsulating quantum dots (QDs) into other medium has been considered as an efficacious method to avoid the fluorescence degradation of QDs. Here, we proposed a facile method to embed CsPbBr3 perovskite QDs in silica matrix derived from (3-aminopropyl)triethoxysilane (APTES) at room temperature in open air. The QDs/SiO2 composite was extracted from the sol-gel solution by using a precipitation-encapsulation method assisted with APTES. As-prepared composite powder possess a high photoluminescence quantum yield (PLQY) of 68% and a full width at half maximum (FWHM) of ~23 nm. The QDs/SiO2 compounds show excellent stability after the five heating cycles (105 °C) and a continuous xenon irradiation (500 W, 70 °C). Besides, the products remain 94.3% and 98.6% photoluminescence (PL) intensity after 30 days of storage (25 °C) and 96 h of ultraviolet (UV) irradiation (λ = 365 nm) respectively. The white light-emitting diode (LED) was fabricated by coating green-emitting CsPbBr3@SiO2 composite powder and commercial red phosphors on blue chip. The fabricated white LED performed excellent light characteristics with a luminous efficacy (ηL) of 58.9 lmW-1 and a correlated color temperature (CCT, Tc) of 5829 K (current = 20 mA), it also display a wide color gamut with 126.8% of National Television System Committee (NTSC) color triangle area.

Supercritical Fluid Media in Challenges of Substance and Material Dispersion

The paper deals with the problem of material dispersion using supercritical fluid media. At the same time, emphasis is made on modifications (SAS, GAS, SEDS and ASES) of the supercritical fluid anti-solvent method of dispersion. The results of SAS method implementation for dispersion of pure polycarbonate and polycarbonate doped with “CdSe/CdS - core/shell” quantum dots (carried out in the pressure range of 8.0-25.0 MPa at temperatures of 313.15 K and 358.15 K) are submitted. The range of the operating parameters has been established through the example of pure polycarbonate dispersion, which provides the production of nanoparticles with the size range of 10 100 nm. Encapsulation of CdSe/CdS quantum dots into polycarbonate using the SAS method has no effect on optical properties of the encapsulated quantum dots. The results of paracetamol dispersion using the SEDS method are presented. The effect of operating conditions of the paracetamol dispersion process on morphology of the obtained product is described. Co-dispersion of ethylene–vinyl acetate copolymers and low-density polyethylene mixtures by SEDS method has been carried out under pressures of 8.0-25.0 MPa at temperatures of 313 K, 323 K, and 333 K. The comparison of melting and crystallization between the resulting copolymer mixtures and mixtures with the same composition obtained by mixing in the liquid melt, has shown that implementation of SEDS results in an increase of crystallinity degree of the polymer mixtures.