Amount Of Oil Research Articles

Application of periodic mode during the operation of oil wells at the late stage of development

Currently, the number of wells operating with a high water cut is growing every year. This leads to a decrease in the profitability of operating oil fields, since the time and energy costs for processing well products increase, and the amount of oil at the output decreases. Thus, optimizing the operating mode of wells in order to reduce water cut by transferring them to periodic mode is one of the key tasks to increase the productivity of oil field development. Oil fields at a late stage of development are characterized by a decrease in oil production volumes and an increase in water cut in well production, as well as a deterioration in the structure of reserves. Residual oil production under difficult conditions plays an important role in maintaining overall field productivity. Low-yield wells can be used to additionally recover oil that was previously unable to be produced during the initial development of wells. In addition, the operation of low-yield wells at a late stage of development makes it possible to reduce the cost of drilling new wells. This article is devoted to the study and updating of this issue. Work continued on the study of converting wells from constant to periodic mode in order to increase technological development indicators, such as reducing the volume of produced water, reducing the specific energy consumption per ton of oil produced and increasing the turnaround time for installing an electric centrifugal pump. An analysis of well operation in constant and periodic modes was carried out for the conditions of the Surgut field. It is shown that transferring the well to periodic mode will reduce the cost of electricity consumption.

[Determination of six halogenated solvent residues in olive oil by headspace gas chromatography].

The residual amount of halogenated solvents in olive oil is an important indicator of its quality. The National Olive Oil Quality Standard GB/T 23347-2021 states that the residual amount of individual halogenated solvents in olive oil should be ≤0.1 mg/kg and that the total residual amount of halogenated solvents should be ≤0.2 mg/kg. COI/T.20/Doc. No. 8-1990, which was published by the International Olive Council, describes the standard method used for the determination of halogenated solvents in olive oil. Unfortunately, this method is cumbersome, has poor repeatability and low automation, and is unsuitable for the detection and analysis of residual halogenated solvents in large quantities of olive oil. At present, no national standard method for determining residual halogenated solvents in olive oil is available in China. Thus, developing simple, efficient, accurate, and stable methods for the determination of residual halogenated solvents in olive oil is imperative. In this paper, a method based on automatic headspace gas chromatography was established for the determination of residual halogenated solvents, namely, chloroform, carbon tetrachloride, 1,1,1-trichloroethane, dibromochloromethane, tetrachloroethylene, and bromoform, in olive oil. The samples were processed as follows. After mixing, 2.00 g (accurate to 0.01 g) of the olive oil sample was added into a 20 mL headspace injection bottle and immediately sealed for headspace gas chromatography analysis. Blank virgin olive oil was used to prepare a standard working solution and the external standard method for quantification. The solvents used in the preparation of halogenated solvent standard intermediates were investigated and methanol was selected as a replacement for N,N-dimethylacetamide to prepare a halogenated solvent standard intermediate owing to its safety. The effects of different injection times (1, 2, 3, 4, 5, 6 s), equilibration temperatures (60, 70, 80, 90, 100, 110, 120 ℃), and equilibration times (4, 5, 8, 10, 20, 30, 40 min) of the headspace sampler on the detection of the residual amounts of the six halogenated solvents were investigated. The optimal injection time and equilibration temperature were 3 s and 90 ℃, respectively. The method demonstrated good analytical performance for the six halogenated solvents when the equilibration time was 30 min. A methodological study was conducted on the optimized method, and the results showed that the six halogenated solvents exhibited good linear relationships in the range of 0.002-0.200 mg/kg, with correlation coefficients of ≥0.9991. The limits of detection (LODs) and quantification (LOQs) of 1,1,1-trichloroethane and bromoform were 0.0006 and 0.002 mg/kg, respectively. The LODs and LOQs of chloroform, carbon tetrachloride, dibromochloromethane, and tetrachloroethylene were 0.0003 and 0.001 mg/kg, respectively. The average recoveries under different spiked levels were 85.53%-115.93%, and the relative standard deviations (n=6) were 1.11%-8.48%. The established method was used to analyze 13 olive oil samples available in the market. Although no halogenated solvents were detected in these samples, a limited number of samples does not represent all olive oils. Hence, monitoring residual halogenated solvents in olive oil remains necessary for its safe consumption. The LOQs of the method for the six halogenated solvents were significantly lower than that of the COI/T.20/Doc. No. 8-1990 standard method (0.02 mg/kg). In addition, the developed method can be conducted under short operation times with high precision and degree of automation as well as good accuracy. Thus, the proposed method is suitable for the determination and analysis of the residues of the six halogenated solvents in large batches of olive oil samples.

Suppression of essential oil biosynthesis in sweet basil cotyledons under hypergravity conditions

The mechanism through which gravity influences the biosynthesis of essential oils in herbs is an important issue for plant and space biology. Sweet basil (Ocimum basilicum L.) seedlings were cultivated under centrifugal hypergravity conditions at 100 g in the light, and the growth of cotyledons, development of glandular hairs, and biosynthesis of essential oils were analyzed. The area and fresh weight of the cotyledons increased by similar amounts irrespective of the gravitational conditions. On the abaxial surface of the cotyledons, glandular hairs, where essential oils are synthesized and stored, developed from those with single-cell heads to those with four-cell heads; however, hypergravity did not affect this development. The main components, methyl eugenol and 1,8-cineole, in the essential oils of cotyledons were lower in cotyledons grown under hypergravity conditions. The gene expression of enzymes in the phenylpropanoid pathway involved in the synthesis of methyl eugenol, such as phenylalanine ammonia lyase (PAL) and eugenol O-methyltransferase (EOMT), was downregulated by hypergravity. Hypergravity also decreased the gene expression of enzymes in the 2C-methyl-d-erythritol 4-phosphate (MEP) pathway involved in the synthesis of 1,8-cineole, such as 1-deoxy-d-xylulose-5-phosphate synthase (DXS) and 1,8-cineole synthase (CINS). These results indicate that hypergravity without affecting the development of glandular hairs, decreases the expression of genes related to the biosynthesis of methyl eugenol and 1,8-cineole, which may cause a decrease in the amounts of both essential oils in sweet basil cotyledons.

Fabrication, characterization and antioxidant activities of pectin and gelatin based edible film loaded with Citrus reticulata L. essential oil

AbstractIn the present work, pectin and gelatin‐based edible films (EFs) loaded with Citrus reticulata L. (tangerine) essential oil were fabricated and evaluated for their potential application in food packaging. GC–MS analysis and physiochemical, mechanical, and antioxidant analysis of the synthesized edible films and oil extract were carried out. GC–MS analysis of the tangerine essential oil revealed the presence of around 40 different chemical constituents, and among them, limonene (43.85%), linalyl acetate (19.16%), linalool (18.38%), and beta‐Myrcene (3.41%) were found as the major constituent. Fourier transform infrared spectroscopy showed the interaction between the functional groups of the film components. Mechanical parameter assessment showed that the tensile strength of the edible film increases and elongation at break values decreases with oil addition. The thickness of the EFs increased with oil addition, while water solubility, water vapor permeability, and transparency decreased. In antioxidant potential assessment assays, maximum activity (DPPH• and ABTS•+ reducing potential) was reported for edible film samples containing a maximum amount (60 μL) of tangerine oil. We found that pectin and gelatin‐based edible films loaded with tangerine essential oil exhibit better characteristics and could be used as a food packaging material.Practical ApplicationsThis study presents a potentially effective method for producing edible films using pectin and gelatin composite, that have been incorporated with tangerine essential oil. These films demonstrate superior properties and may serve as an effective choice for food packaging material.

From Wild Palm to Plantation System: A Trajectory of Colonial Policies on Palm Oil Production in Annang, Nigeria, 1900-1960

This paper examines colonial economic policies and the oil palm production in Annang, of Calabar Province of Nigeria from 1901-1960. Before the establishment of colonial rule, the people of Annang were engaged in the production of wild oil palm for domestic use. The colonial period witnessed the introduction of plantation system for the cultivation oil palm trees and production of palm oil for export. Annang produced large quantities of palm oil and thus contributed greatly to colonial economy. In spite this, the contribution of Annang to colonial economy has over the years been treated as as an appendage to the Ibibio colonial historical experiences. The neglect is what this article attempts to correct. Oil palm tree, has a long history in Annang, especially as most its byproducts served both domestic and commercial needs of the people. This article examines Annang unique experiences during colonial period in the light of the fast-developing interest in micro studies in Nigerian historiography. It therefore focuses on the colonial policies on palm oil production, especially the various measures formulated and implemented in Annang from the inception of British colonial rule in Annang in 1901 to 1960 when Nigeria attained independence. It adopts the historical method, relying extensively on primary sources obtained from oral interviews and archival sources to interrogate palm oil production in Annang during the period under consideration. Keywords: Oil palm Trees, Palm Oil, Plantation System, and Colonial Policies.

Влияние заменителей рыбьего жира в кормах сиговых рыб (Coregonidae) на рост, физиологические показатели и жирнокислотный состав липидов

The results of a study on the replacement of fish oil in the feed for two-year-old whitefish with other sources of lipids have been carried out. When replacing a fifth of the fish oil (4 out of 20%) with flaxseed oil, there was no significant difference in growth, fish survival, physiological parameters, and fatty acid composition of lipids. In the following experiments, fish oil was changed to linseed, soybean, rapeseed and camelina oils. When replacing 5, 7, 10 and 15 of 20% of fish oil with flaxseed oil, the content of omega-3 acids in fish muscles increased from 9 to 27%, including the sum of long-chain acids – docosahexaenoic (DHA) and eicosapentaenoic (EPA) – from 2.4 to 23.5%, which demonstrates the ability of whitefish to transform maternal alpha-linolenic acid, which is rich in linseed oil, into long-chain polyunsaturated acids. At the same time, the amount of monounsaturated oleic acid decreased from 41.4 to 21.7%. An improvement in the fatty acid composition of feed when vegetable oils were introduced instead of fish oil indicates its low quality, as indicated by high mortality, a decrease in vitamin C, and a deterioration in the fatty acid composition of lipids in fish that received food with the highest amount of fish oil. Based on the consideration of all indicators, the best results were obtained when replacing half of the fish oil with linseed oil (10% out of 20%). At the same time, the quality of fish products was preserved. The amount of DHA and EPA in 100 g of farmed fish muscle was 0.89 g, which satisfies the daily human need for these important omega-3 acids for health. When replacing fish oil with camelina, soybean and rapeseed oils, the results were slightly worse.

Chitosan-Oxidized Pullulan Hydrogels Loaded with Essential Clove Oil: Synthesis, Characterization, Antioxidant and Antimicrobial Properties.

Emulsion hydrogels are promising materials for encapsulating and stabilizing high amounts of hydrophobic essential oils in hydrophilic matrices. In this work, clove oil-loaded hydrogels (CS/OP-C) are synthesized by combining covalent and physical cross-linking approaches. First, clove oil (CO) was emulsified and stabilized in a chitosan (CS) solution, which was further hardened by Schiff base covalent cross-linking with oxidized pullulan (OP). Second, the hydrogels were subjected to freeze-thaw cycles and, as a result, the clove oil was stabilized in physically cross-linked polymeric walls. Moreover, due to cryogelation, the obtained hydrogels exhibited sponge-like porous interconnected morphology (160-250 µm). By varying the clove oil content in the starting emulsion and the degree of cross-linking, the hydrogels displayed a high water retention capacity (swelling ratios between 1300 and 2000%), excellent elastic properties with fast shape recovery (20 s) after 70% compression, and controlled in vitro clove oil release in simulated skin conditions for 360 h. Furthermore, the prepared clove oil-loaded hydrogels had a strong scavenging activity of 83% and antibacterial and antifungal properties, showing a bacteriostatic effect after 48 and 72 h against S. aureus and E. coli. Our results recommend the new clove oil-embedded emulsion hydrogels as promising future materials for application as wound dressings.

Comparison of elastic anisotropy in the Middle and Upper Wolfcamp Shale, Midland Basin

AbstractOrganic‐rich shales contain large amounts of oil and gas and are anisotropic because of fine‐scale layering and the partial alignment of organic matter and anisotropic clay minerals with the bedding. An example is the Wolfcamp Shale in the Permian Basin. Elastic anisotropy needs to be accounted for in the characterization of such formations using seismic data and plays a role in hydraulic fracturing and in the evaluation of stress changes and geomechanical effects resulting from production. Using extensive well log data acquired in the Midland Basin, the eastern sub‐basin of the Permian Basin, we estimate and compare the elastic anisotropy in the Middle and Upper Wolfcamp Shale by combining data from a vertical pilot well with two lateral wells, one (6SM) drilled in the Middle Wolfcamp and one (6SU) drilled in the Upper Wolfcamp. The data used were acquired at the Hydraulic Fracture Test Site 1, located in the eastern part of the Midland Basin. Thomsen's anisotropy parameter calculated from the fast and slow shear sonic is higher on average for the 6SM lateral than for 6SU, consistent with there being less carbonate content in 6SM than in 6SU. However, the anisotropy parameter in some regions with higher carbonate content in well 6SU is higher than in well 6SM. This may indicate the influence of natural fractures. The primary set of steeply dipping fractures observed in the lateral wells at Hydraulic Fracture Test Site 1 acts to increase if the ratio of the normal‐to‐shear fracture compliance is less than about 0.5. Sub‐horizontal fractures may also increase and could affect the vertical extent of hydraulic fractures. Relations between elastic moduli C33 and C55 in the Upper and Lower Wolfcamp in a vertical pilot well allow C33 to be predicted in a lateral well using measurements of C55 in that well. Comparison of Thomsen's anisotropy parameters and , with calculated from the measured values of C55 and C66 and calculated from the measured values of C11 and predicted values of C33, show that  is mostly greater than .

Co-enzymatic hydrolysis enhanced thermal stability of mayonnaise

With the accelerated pace of life, frozen bakery products and ready-to-eat hot food are gradually emerging. Mayonnaise, a commonly used flavor sauce, is also applied to these products. However, with the large amount of oil and fat precipitation of mayonnaise in the reheating process of the product, which affects its application in the food industry, the thermal stability of mayonnaise has been gradually paid attention to. In this study, the thermal stability of mayonnaise was effectively improved by the combined enzymatic hydrolysis of liquid egg yolk (LEY) with phospholipase A2 (PLA2) and flavor protease. It was found that the moderate addition of flavor protease could effectively reduce the surface hydrophobicity and interfacial tension of egg yolk protein and improve its emulsification stability. Moreover, the hydrolysis reduced the molecular weight of yolk protein, thus reducing the thermal aggregation effect of yolk protein due to heating, which in turn reduced the loss of protein at the O/W interface and improved the thermal stability of mayonnaise.

Construction of a soybean protein isolate/polysaccharide-based whole muscle meat analog: Physical properties and freeze-thawing stability study

A growing interest has arisen in recreating real meat by mimicking its texture characteristics and muscle fiber structure. Our previous work successfully created meat analog fiber based on soybean protein isolate (SPI) and sodium alginate (SA) with the wet-spinning method. In this work, we analyzed the microstructure, texture profile, and water retainability of the assembled plant-based whole muscle meat analog (PMA) made of SPI/SA-based meat analog fiber and systematically studied the effect of different combinations and contents of transglutaminase (TG), salt, and soybean oil on the rheological behavior of the formulated adhesive. The estimated optimal condition that has the most similar texture characteristic with real chicken breast meat is: for every 1:1 mass ratio of simulated plant meat fibers to the adhesive, add 0.1 % TG enzyme addition in the adhesive and 100 mM NaCl addition. The physical behavior of PMA during cryopreservation was investigated through freeze-thaw cycles and freezing times. The addition of a small amount of oil and salt can efficiently prevent the PMA through freezing conditions which is comparable with the addition of D-Trehalose (TD). Overall, this study not only created a plant-based whole muscle meat analog product that is similar in texture to real chicken breast meat but also provided a new direction for constructing fiber-rich structure protein-based muscle meat analogs and their further commercialization.

Aqueous dispersions of oleic acid nanodroplets for thymol encapsulation

This study focuses on the use of oleic acid to disperse thymol as nanodroplets, stabilized by a mixture of alkylpolyglucoside and lecithin, in an aqueous environment. This approach aims to develop innovative platforms for the encapsulation and release of poorly water-soluble molecules such as thymol, useful for drug delivery and insecticide systems. The results highlight the critical role of controlling the content and concentration of the oil phase (thymol-oleic acid mixture) in achieving optimal thymol dispersion and nanodispersion stability. The interplay between the ability of oleic acid to inhibit thymol crystallization and the maximum dispersible oil amount is crucial. It affects the dispersion of thymol within the nanodroplets and influences coalescence and Ostwald ripening phenomena. The balance between oleic acid and thymol content is key: while oleic acid stabilizes dispersions, higher thymol content increases droplet size, potentially triggering destabilization. The uneven distribution of thymol within the droplets, revealed by fluorescence spectroscopy, suggests that up to three different chemical environments exist. This investigation may pave the way for the development of efficient platforms to improve access to biologically relevant, poorly soluble molecules.

Oil spill risk analysis for the NEOM shoreline

A risk analysis is conducted considering an array of release sources located around the NEOM shoreline. The sources are selected close to the coast and in neighboring regions of high marine traffic. The evolution of oil spills released by these sources is simulated using the MOHID model, driven by validated, high-resolution met-ocean fields of the Red Sea. For each source, simulations are conducted over a 4-week period, starting from first, tenth and twentieth days of each month, covering five consecutive years. A total of 180 simulations are thus conducted for each source location, adequately reflecting the variability of met-ocean conditions in the region. The risk associated with each source is described in terms of amount of oil beached, and by the time required for the spilled oil to reach the NEOM coast, extending from the Gulf of Aqaba in the North to Duba in the South. To further characterize the impact of individual sources, a finer analysis is performed by segmenting the NEOM shoreline, based on important coastal development and installation sites. For each subregion, source and release event considered, a histogram of the amount of volume beached is generated, also classifying individual events in terms of the corresponding arrival times. In addition, for each subregion considered, an inverse analysis is conducted to identify regions of dependence of the cumulative risk, estimated using the collection of all sources and events considered. The transport of oil around the NEOM shorelines is promoted by chaotic circulations and northwest winds in summer, and a dominant cyclonic eddy in winter. Hence, spills originating from release sources located close to the NEOM shorelines are characterized by large monthly variations in arrival times, ranging from less than a week to more than 2 weeks. Similarly, large variations in the volume fraction of beached oil, ranging from less then 50% to more than 80% are reported. The results of this study provide key information regarding the location of dominant oil spill risk sources, the severity of the potential release events, as well as the time frames within which mitigation actions may need to deployed.

Research on loss rules of oil and gas in preserved shale cores after open air exposure

There is a large amount of oil and gas loss in traditional conventional core samples. Revealing the rules of oil and gas loss is of great significance for restore the pristine oil content and oil component in the shale. In this study, four preserved shale cores with different thermal maturity (Ro = 1.01–1.53%) and different total organic carbon content (TOC = 1.69–5.48 wt.%) were selected. The samples are obtained from the first member of the Qingshankou Formation in the Gulong Sag. Nuclear magnetic resonance (NMR) T1–T2 mapping and thermal desorption gas chromatography (TD–GC, at a constant temperature of 300°C for 3 min) were performed on the preserved cores and their replicas that were exposed in open air for different times, to study dynamic loss process and the molecular composition changes of shale oil. The results show that during exposure, shale experiences a large amount of oil loss, with a loss ratio of about 42%–78%, and the higher the maturity, the greater the loss ratio. The oil loss is mainly contributed by free oil, with a loss ratio as high as 88%. The adsorbed oil content, however, remains basically unchanged and has a good positive correlation with the TOC of shale. Once the cores were crushed, the gaseous hydrocarbon in oil was basically evaporated in just 5 min. After long-term storage, 90% of the C14- light hydrocarbon is lost, while the C14+ heavy hydrocarbon experiences basically no loss. Therefore, effective and timely analysis of preserved shales is extremely important. The oil content of uncrushed shale cores characterized by NMR T1–T2 mapping is much greater than that of the crushed sample measured by TD-GC, which means that NMR T1–T2 mapping can be important method to evaluate the original fluid saturation of shale.

A Laboratory Experimental Study on Enhancing the Oil Recovery Mechanisms of Polymeric Surfactants.

In order to evaluate the physical and chemical properties of polymer surfactants and analyze their oil displacement mechanisms, three types of poly-surfactant used in the Daqing oil field were chosen to be researched, and the oil displacement effects were studied using poly-surfactants of different viscosity, dehydrating rate, and core permeability. The main purpose is to determine the reasonable range of different characteristic indexes of polymeric surfactant flooding. The oil displacement effect of 15 cores was analyzed, and the effects of viscosity, the dehydrating rate of emulsion, and permeability on EOR (Enhanced Oil Recovery) were analyzed. The oil displacement mechanisms of polymeric surfactants were researched using a photolithographic glass core. This paper explores the mechanism underlying production enhancement as an EOR target, while simultaneously conducting laboratory tests to assess the physical and chemical properties of polymeric surfactants. The poly-surfactant agents exhibit a notable increase in viscosity, with the optimal displacement effect observed at a core effective permeability exceeding 400 mD, resulting in a potential EOR of 15% or higher. Moreover, at a viscosity ranging between 40 and 70 mPa·s, the total EOR can reach 73%, with the peak efficiency occurring at a viscosity of 60 mPa·s. The water loss rate of the emulsion, ranging between 30% and 70%, achieves optimal performance at 50%. The poly-surfactants' higher viscosity extends the oil sweep area, enhancing recovery efficiency, and noticeably reducing residual oil compared to water flooding. During poly-surfactant flooding, a substantial amount of residual oil is extracted and transformed into droplets. The rapid emulsification of the polymeric surfactant solution with crude oil forms a stable emulsion, contributing to its significant oil recovery effect. This research provides valuable technical support for EOR in thin and low-quality reservoirs of onshore multi-layered sandstone reservoirs.

White mustard (Sinapis alba L.) seeds: the role of oven and microwave roasting on their bioactive components, antioxidant potential, fatty acids and mineral contents

Abstract The oil amounts of raw and roasted mustard seeds were reported between 12.05 (control) and 16.30% (oven). Total phenolic and flavonoid amounts of untreated (control) and roasted mustard seeds were recorded to be between 473.90 (control) and 569.96 mgGAE/100 g (oven) to 345.48 (control) and 479.76 mg/100 g (microwave), respectively. Antioxidant capacity results of untreated and roasted mustard seeds were determined to be between 6.71 (oven) and 6.97 mmol/kg (control). Total phenolic and flavonoid amounts of oven-roasted seeds were higher than those of control and microwave-roasted seeds. L* values of mustard seeds varied between 35.04 and 65.52. Heat treatment caused a decrease in L* values. The lowest L* value was observed in the sample dried in conventional oven. The quantitative values of gallic and 3,4-dihydroxybenzoic acids of mustard seeds were recorded to be between 8.47 (control) and 117.31 mg/100 g (microwave) to 2.16 (control) and 11.79 mg/100 g (microwave), respectively. Erucic acid values of the oils extracted from raw and roasted mustard seeds were reported between 41.38 (control) and 42.81% (microwave). Oleic and linoleic acid amounts of mustard oils differed between 26.06 (microwave) and 26.90% (control) and between 13.08 (oven) and 13.98% (control), respectively. The oven roasting system had the most effect on the element and protein contents of the seeds, followed by the microwave in decreasing order. As with the bioactive components of the seeds, the amounts of phenolic constituents of roasted mustard seeds increased significantly compared to the control. Graphical abstract

Effect of Different Light Qualities on Essential Oil and Asarinin in Asarum heterotropoides Fr. Schmidt var. mandshuricum (Maxim.) Kitag

Asarum heterotropoides Fr. var. mandshuricum (Maxim.) is a perennial herb native to China. Its essential oil components and asarinin are health-promoting compounds. However, cultivation under natural light can affect the growth and secondary metabolite yield of this sciophyte. Adjustment of light irradiance may be beneficial in this respect. Here, we applied six types of filtered solar irradiance by using filter films of red (I), yellow (II), blue (III), green (IV), purple (V) and 50% sunlight (VI). We measured net photosynthetic rate (APn), activity of phenylpropanoid biosynthetic enzymes, asarinin content, and essential oil content and composition. Light quality treatments V and VI resulted in increased APn, enzyme activities, asarinin content and essential oil content. Samples harvested in September 2020 had relatively higher contents and enzyme activities compared to those harvested in July 2020. Elemicin, 2,5-dimethoxytoluene and methyleugenol accumulated at higher levels in response to light quality V. Light qualities III and IV showed promising results for several compounds. Overall, we conclude that controlling the light conditions in growing environments promotes the amount of asarinin and essential oil by regulating photosynthesis and the activities of phenylpropanoid biosynthetic enzymes.

COMPONENT COMPOSITIONS OF THE ESSENTIAL OIL AND PHENOLIC COMPOUNDS OF THE AERIAL PART OF LOPHANTHUS SCHTSCHUROVSKIANUS

The component composition of the essential oil (EO) obtained by hydrodistillation from the air dried aerial part of the Lophanthus schtschurowskianus (Regel) Lipsky, growing in the Surkhandarya region of the Republic of Uzbekistan, was studied. By the GC-MS method were identified 57 components in the composition of EO, which was 93.8% of the total amount of oil, of which 52 volatile compounds were identified. The main components of the essential oil were 1,8-cineol (13.4%), viridiflorol (8.5%), α-terpineol (4.3%), terpinen-4-ol (4.2%), τ-cadinol (4.1%), β-spatulenol (3.9%), α-p-dimethylstyrene (2.1%). Oxygenated monoterpenes (33.6%) and oxygenated sesquiterpenes (24.7%) predominate in the composition of the essential oil; compounds related to sesquiterpene hydrocarbons (8.6%) were also found. The composition of the EO components of L. schtschurowskianus significantly differs from the EOs of other studied plant species of the genus Lophanthus.
 The antibacterial and antifungal activity of the essential oil, as well as various extracts from the aerial parts of L. schtschurowskianus, was studied using a modified agar diffusion method. Gram-positive bacteria were found to be susceptible to all the studied samples. Among the studied samples, the essential oil showed the highest antibacterial activity against Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli.
 Three individual phenolic compounds were isolated from various fractions of a 75% alcoholic extract of the aerial part of L. schtschurowskianus, which, based on the study of 1H, 13C NMR, HSQC and HMBC spectra, were identified as caffeic, rosmarinic acids and luteolin.

The Sustainability of Palm Oil

The most significant environmental issue associated with palm oil that is accused by the European Union is deforestation, particularly in Indonesia and Malaysia. The tension arose after the EU established Renewable Energy Directive II. EU considered palm oil-producing countries have ignored environmental issues by expanding palm oil plantations which has resulted in deforestation. This made palm oil plantations included in the high-risk Indirect Land Use Change (ILUC) category. Therefore, this article presents a comparison of the sustainability between palm oil and other vegetable oils such as sunflower oil, rapeseed oil, etc. The findings of this article contextualize the comparative advantage of palm oil over other similar resources. The reasons why palm oil is more sustainable are it needs less land to produce the same amount of oil, its sustainability certification at the international level such as RSPO which is also connected to SDGs, and at the domestic level such as ISPO and MSPO. One strategy proposed in this article is instead of boycotting palm oil, the government and stakeholders should prioritize the implementation of sustainable practices, enhance the monitoring and enforcement of RSPO scheme standards, and tackle social concerns and deforestation that are always linked to palm oil.

Triacylglycerol stability limits futile cycles and inhibition of carbon capture in oil-accumulating leaves.

Engineering plant vegetative tissue to accumulate triacylglycerols (TAG, e.g., oil) can increase the amount of oil harvested per acre to levels that exceed current oilseed crops. Engineered tobacco (Nicotiana tabacum) lines that accumulate 15% to 30% oil of leaf dry weight resulted in starkly different metabolic phenotypes. In-depth analysis of the leaf lipid accumulation and 14CO2 tracking describe metabolic adaptations to the leaf oil engineering. An oil-for-membrane lipid tradeoff in the 15% oil line (referred to as HO) was surprisingly not further exacerbated when lipid production was enhanced to 30% (LEC2 line). The HO line exhibited a futile cycle that limited TAG yield through exchange with starch, altered carbon flux into various metabolite pools and end products, and suggested interference of the glyoxylate cycle with photorespiration that limited CO2 assimilation by 50%. In contrast, inclusion of the LEAFY COTYLEDON 2 (LEC2) transcription factor in tobacco improved TAG stability, alleviated the TAG-to-starch futile cycle, and recovered CO2 assimilation and plant growth comparable to wild type but with much higher lipid levels in leaves. Thus, the unstable production of storage reserves and futile cycling limit vegetative oil engineering approaches. The capacity to overcome futile cycles and maintain enhanced stable TAG levels in LEC2 demonstrated the importance of considering unanticipated metabolic adaptations while engineering vegetative oil crops.

EXPERIMENTAL STUDY OF FLOW PATTERNS AND PRESSURE LOSSES IN VERTICAL FLOWS OF OIL-WATER MIXTURES

In this work the experimental results of the dynamics of the two-phase oil-water flow patterns and the pressure gradients due to friction that they produce when conducting them in a vertical ascending pipe are shown. For the study, an experimental facility and two types of injection nozzles were built, constructed and characterized. The selected fluids were oil (viscosity 90 cP, density 885 kg/m3 with 28.3 API) and water. The measurements were made in a range of superficial speeds from 0 to 0.257 m/s for oil and from 0 to 0.684 for water. The experimentation was carried out using the two nozzles and in accordance with a test matrix that has as parameters the superficial speed of the oil (USO) and the superficial speed of the water (USW) for which a fixed value of the USW was taken and it varied the USO, although it was also worked in reverse. From the experimental results, the effect of the nozzles to induce the group of flow patterns and their respective pressure gradient is shown, which reaches its lowest value when conducting the oil in the annular flow scheme. The numerical results showed that there is a notable energy saving, since the pressure loss due to friction is up to 5 times lower when conducting the same amount of oil in the annular flow pattern. Key Words: Experimental installation, oil-water mixture, injection nozzle, flow patterns, annular flow, pressure gradient.