Quantitative Reconstruction Research Articles

Group 2i Isochrysidales thrive in marine and lacustrine systems with ice cover

Global warming is causing rapid changes to the cryosphere. Predicting the future trajectory of the cryosphere requires quantitative reconstruction of its past variations. A recently identified sea-ice-associated haptophyte, known as Group 2i Isochrysidales, has given rise to a new sea-ice proxy with its characteristic alkenone distributions. However, apart from the occurrence of Group 2i Isochrysidales in regions with sea ice, and the empirical relationship between C37:4 alkenone abundance and sea-ice concentration, little is known about the ecology of these haptophyte species. Here, we systematically mapped the spatial and temporal occurrence of known Group 2i Isochrysidales based on environmental DNA in both marine and lacustrine environments. Our results indicate Group 2i is widely distributed in icy marine and lacustrine environments in both Northern and Southern Hemisphere, but is absent in warm environments. Temporally, Group 2i is part of the sea-ice algae bloom during the cold seasons, in contrast to other Isochrysidales that bloom in open waters during warm seasons. Our results indicate that ice is a prerequisite for the occurrence of the psychrophilic Group 2i haptophytes in marine and lacustrine ecosystems and further affirms its value for past ice reconstructions.

Changes in monsoon precipitation in East Asia under a 2°C interglacial warming.

Past intervals of warming provide the unique opportunity to observe how the East Asia monsoon precipitation response happened in a warming world. However, the available evaluations are primarily limited to the last glacial-to-interglacial warming, which has fundamental differences from the current interglacial warming, particularly in changes in ice volume. Comparative paleoclimate studies of earlier warm interglacial periods can provide more realistic analogs. Here, we present high-resolution quantitative reconstructions of temperature and precipitation from north-central China over the past 800 thousand years. We found that the average precipitation increase, estimated by the interglacial data, was only around one-half of that estimated for the glacial-to-interglacial data, which is attributed to the amplification of climate change by ice volume variations. Analysis of the interglacial data suggests an increase in monsoon precipitation of ~100 mm for a warming level of 2°C on the Chinese Loess Plateau.

Nuclear patterns of phosphatidylinositol 4,5- and 3,4-bisphosphate revealed by super-resolution microscopy differ between the consecutive stages of RNA polymerase II transcription.

Phosphatidylinositol phosphates are powerful signaling molecules that orchestrate signaling and direct membrane trafficking in the cytosol. Interestingly, phosphatidylinositol phosphates also localize within the membrane-less compartments of the cell nucleus, where they participate in the regulation of gene expression. Nevertheless, current models of gene expression, which include condensates of proteins and nucleic acids, do not include nuclear phosphatidylinositol phosphates. This gap is partly a result of the missing detailed analysis of the subnuclear distribution of phosphatidylinositol phosphates and their relationships with gene expression. Here, we used quantitative dual-color direct stochastic optical reconstruction microscopy to analyze the nanoscale co-patterning between RNA polymerase II transcription initiation and elongation markers with respect to phosphatidylinositol 4,5- or 3,4-bisphosphate in the nucleoplasm and nuclear speckles and compared it with randomized data and cells with inhibited transcription. We found specific co-patterning of the transcription initiation marker P-S5 with phosphatidylinositol 4,5-bisphosphate in the nucleoplasm and with phosphatidylinositol 3,4-bisphosphate at the periphery of nuclear speckles. We showed the specific accumulation of the transcription elongation marker PS-2 and of nascent RNA in the proximity of phosphatidylinositol 3,4-bisphosphate associated with nuclear speckles. Taken together, this shows that the distinct spatial associations between the consecutive stages of RNA polymerase II transcription and nuclear phosphatidylinositol phosphates exhibit specificity within the gene expression compartments. Thus, in analogy to the cellular membranes, where phospholipid composition orchestrates signaling pathways and directs membrane trafficking, we propose a model in which the phospholipid identity of gene expression compartments orchestrates RNA polymerase II transcription.

Imaging the Magnetic Anisotropy in Ultrathin Fe4GeTe2 with a Nitrogen-Vacancy Magnetometer.

Two-dimensional (2D) FenGeTe2, with n = 3, 4, and 5, has been realized in experiments, showing strong magnetic anisotropy with enhanced critical temperature (Tc). The understanding of its magnetic anisotropy is crucial for the exploration of more stable 2D magnets and its spintronic applications. Here, we report a quantitative reconstruction of the magnetization magnitude and its direction in ultrathin Fe4GeTe2 using nitrogen vacancy centers. Through imaging stray magnetic fields, we identified the spin-flop transition at approximately 80 K, resulting in a change of the easy axis from the out-of-plane direction to the in-plane direction. Moreover, by analyzing the thermally activated escape behavior of the magnetization near Tc in terms of the Ginzburg-Landau model, we observed the in-plane magnetic anisotropy effect and the formation capability of magnetic domains at ∼0.4 μm2 μT-1. Our findings contribute to the quantitative understanding of the magnetic anisotropy effect in a vast range of 2D van der Waals magnets.

Quantification of Holocene temperatures in the eastern Hunshandake Sandy Land using δ13C of loess organic matter

The soil organic carbon isotope indicator (δ13Corg) is widely used in paleoecology and paleoclimate reconstruction due to its ability to record the history of paleovegetation change. Although it has been used as a proxy for precipitation reconstruction, increasing evidence suggests that temperature has played a crucial role in influencing the relative abundance of C3/C4 plants in the mid-latitudes during the interglacial period. This opens up the possibility of reconstructing Holocene temperature using δ13Corg. In this study, the variation of δ13Corg values in a Holocene loess profile (GLH profile), located in the eastern Hunshandake Sandy Land, China, was investigated. And we analyzed the correlation between δ13Corg values and modern temperatures for a total of 792 surface soil samples from mid-latitudes of the Northern Hemisphere, which had thermal conditions comparable to the GLH profile. Strong positive correlations were found between δ13Corg values of surface soil samples and both summer temperatures (r = 0.672, p < 0.001) and growing season temperatures (r = 0.669, p < 0.001). Additionally, δ13Corg values showed significant positive correlations with accumulated temperatures above 10 °C (r = 0.641, p < 0.001) and above 0 °C (r = 0.527, p < 0.001). However, the correlation with mean annual temperature was weak (r = 0.280, p < 0.001). The δ13Corg variation in the GLH profile recorded a mixed C3/C4 vegetation composition, with C4 plants comprising between 23.40% and 1.70% of the total abundance during the Holocene. The combined evidence from modern processes and profile variations confirms that warm-season temperature was the main factor driving variations in the δ13Corg values in the GLH profile. Therefore, we used δ13Corg–temperature conversion equations to quantify Holocene seasonal and accumulated temperatures based on the GLH profile. The reconstructed summer temperatures peaked at 9.3 ka, followed by a fluctuating downward trend during the mid- and late-Holocene, which is consistent with variations in summer solar insolation. Mean summer temperatures were relatively high at 23.3 °C and 22.5 °C in the early and mid-Holocene, respectively, while the lowest mean summer temperature of 20.5 °C occurred in the late Holocene. Accumulated temperatures above 10 °C and above 0 °C in the early Holocene were 3722 °C·d and 4641 °C·d, respectively, similar to modern observations in the Beijing area, indicating a southward shift of the temperature zone by nearly three degrees of latitude since the early Holocene. These reconstruction results are supported by modern observations, TraCE21 simulations, and other studies. This study is significant for extending the application of the δ13Corg indicator for quantitative paleoclimate reconstructions. It verifies its effectiveness in quantifying Holocene temperatures in the marginal zone of the East Asian summer monsoon.

3D genomic mapping reveals multifocality of human pancreatic precancers.

Pancreatic intraepithelial neoplasias (PanINs) are the most common precursors of pancreatic cancer, but their small size and inaccessibility in humans make them challenging to study1. Critically, the number, dimensions and connectivity of human PanINs remain largely unknown, precluding important insights into early cancer development. Here, we provide a microanatomical survey of human PanINs by analysing 46 large samples of grossly normal human pancreas with a machine-learning pipeline for quantitative 3D histological reconstruction at single-cell resolution. To elucidate genetic relationships between and within PanINs, we developed a workflow in which 3D modelling guides multi-region microdissection and targeted and whole-exome sequencing. From these samples, we calculated a mean burden of 13 PanINs per cm3 and extrapolated that the normal intact adult pancreas harbours hundreds of PanINs, almost all with oncogenic KRAS hotspot mutations. We found that most PanINs originate as independent clones with distinct somatic mutation profiles. Some spatially continuous PanINs were found to contain multiple KRAS mutations; computational and in situ analyses demonstrated that different KRAS mutations localize to distinct cell subpopulations within these neoplasms, indicating their polyclonal origins. The extensive multifocality and genetic heterogeneity of PanINs raises important questions about mechanisms that drive precancer initiation and confer differential progression risk in the human pancreas. This detailed 3D genomic mapping of molecular alterations in human PanINs provides an empirical foundation for early detection and rational interception of pancreatic cancer.

Climatic variability in the Armenian Highlands as the backdrop to hominin population dynamics 50–25 ka

The impact of Late Pleistocene climatic oscillations, volcanism and the diverse terrain of the Armenian Highlands affected hominin population dynamics and movements through the region. To test different scenarios for the period 50–25 ka regarding expansion, adaptive response, intra-population interactions and extinction, we need local on-site paleoclimatic data found in association with hominin occupations. However, this approach has been hampered by the dearth and highly uneven spatiotemporal recovery of paleoclimatic data from prehistoric sites across the region. In this study, we analyze multiple assemblages of fossil micromammals from the Late Middle Paleolithic (LMP) Lusakert-1 rockshelter (two fossil-yielding strata; <55 ka) and Early Upper Paleolithic (EUP) Aghitu-3 Cave (four fossil-yielding strata; ∼39–24 ka) using geometric morphometric-aided taxonomy, to quantify the representation of different species among the abundant and diverse arvicoline genera (Microtus and Chionomys; mean = 58% of assemblages). We combined this approach with quantitative species-level paleoclimatic reconstructions (Bioclimatic Model; 14 rodent taxa) to determine changes in habitat composition as well as temperature and precipitation shifts during the Late Middle–Early Upper Paleolithic. Taphonomic analysis was conducted to establish isotaphonomy among the assemblages. The analysis shows higher than present-day absolute temperatures for the series of occupations at both Lusakert-1 and Aghitu-3, making it unlikely that hominin activity at the sites coincided with extreme cold-climate phases. A multivariate comparison of the taxonomic abundances (genus level) across a wider sample of regional Middle Paleolithic (MP) and Upper Paleolithic (UP) cave sites (Hovk-1, Kudaro-1, Mezmaiskaya, Satsurblia and Dzudzuana) indicates the possibility of cold-period occupation at only two of the sites, Mezmaiskaya Layers 3 and 2 and Kudaro-1 Layer 3. We surmise that the manufacturers of LMP lithic industries in the Caucasus were well adapted to these challenging environments, thus undermining arguments that rapid climate change through the LMP/EUP period could alone have determined the course of population dynamics in this region.

Asian monsoon variations over the past 21 ka: An introduction

The Asian monsoon influences the lives of more than a third of the world's population, and understanding its spatial and temporal variability is fundamental to sustainable development. The complex dynamics of this climatic system are therefore of broad scientific and societal interest. In this virtual special issue (VSI), we present 19 papers dealing with the Asian monsoon since the Last Glacial Maximum (LGM, ∼21 kyr BP), with a focus on understanding variability and forcing mechanisms. Findings use recently developed quantitative reconstructions combined with numerical simulations, and present novel insights as follows: (1) analyses of comprehensive datasets of pollen, diatom, brGDGTs, oxygen and hydrogen isotopes and frequency magnetic susceptibility, as well as an innovative algorithm model for the lake energy-water balance, improve the reliability of quantitative reconstructions of Asian monsoon climate variations; (2) studies of quantitative climate parameters in central East Asia show that mean annual temperature (MAT) was ∼5 °C (or ∼ 7.9 °C using brGDGTs) lower during the LGM, ∼1.5 °C (±41% using brGDGTs) higher around the Holocene thermal optimum (HO) and mean annual precipitation (MAP) varied between 30 and 150% with marked seasonality during 8–5 ka; (3) dynamical downscaling palaeoclimatic simulations improve the accuracy of past monsoon variations, revealing strong variability in monsoon precipitation across the LGM and HO. The holistic study of Asian monsoon variations at orbital to centennial timescales in these VSI papers presents reliable observations of monsoon variability and its driving mechanisms, providing high-resolution quantitative paleoclimate reconstructions that fill existing gaps in spatial coverage, as well as offering an improved understanding, thorough numerical simulation, of coupled sea-level change and vegetation feedbacks.

Relative Pollen Productivity Estimates for Mediterranean Plant Taxa: A New Study Region in Turkey

This study estimates relative pollen productivity (RPP) for plant taxa from Southern Anatolia, an important region in the Mediterranean with a long history of human settlements. RPP estimates are required for quantitative pollen-based reconstruction of past land cover modelling. The application of the reconstruction by the REVEALS model in the Mediterranean basin is constrained due to the scarcity of the RPP values specific to the region. To better understand the relationship between vegetation cover and land use in the Mediterranean area, the present study aims to provide a set of RPPs for Turkey and the Mediterranean region. The study area centres around Gölhisar Lake in southwestern Turkey. Modern pollen data are collected from moss pollsters from 21 sites together with vegetation surveys. RPP estimates for the main taxa characteristic of the Mediterranean region are obtained (referenced to evergreen Quercus t.) using the extended R-value (ERV) model through the analysis of modern pollen assemblages. The most reliable results are acquired with the ERV sub-model 2 and Prentice’s taxon-specific method (using a Gaussian plume dispersal model) to distance-weighted vegetation data, corresponding to a Relative Source Area of Pollen (RSAP) value of 102 m. RPPs of dominant taxa in the study area are obtained for Quercus coccifera/Fagaceae (1 ± 0), Juniperus/Cupressaceae (0.279 ± 0.001), Fabaceae (0.008 ± 0.000), Pinus/Pinaceae (5.782 ± 0.011), and Poaceae (0.112 ± 0.001) and are comparable with other RPPs obtained in the Mediterranean region.

The environmental and cultural background for the reoccupation of the Armenian Highlands after the Last Glacial Maximum: The contribution of Kalavan 6

This paper introduces the results from our excavations of the open-air late Upper Paleolithic site of Kalavan 6, Armenia. The site is embedded in a sedimentological sequence spanning from the Last Glacial Maximum (LGM; 26.5–19/20 ka) to the Holocene (i.e., from MIS 2 to 1). Our findings are presented together with chronological, environmental, and climatic data. Luminescence dating provides a temporal framework for reoccupation of the Armenian Highlands after the LGM, while two vegetation proxies (pollen assemblages and leaf waxes) characterize the environment. Based on these pollen data, a quantitative climate reconstruction (temperatures and precipitation) is offered. Techno-typological characterization of the lithic assemblages is presented together with the sourcing of the entire obsidian assemblage by portable X-ray fluorescence, providing insights into the occupants’ exploited territories. Such a framework, which incorporates both environmental reconstruction and hunter-gatherer behaviors, enables us to contextualize possible links between population dynamics during the height of the LGM and post-LGM environmental oscillations. We suggest that, during the LGM between ca. 24 and 19 ka, the combination of declining temperatures and the extension of the winter season limited the occupation feasibility of the region. The regional occupation resumed when environmental circumstances ameliorated. These results support our interpretation that temperatures and the duration of the seasons conditioned the past hunter-gatherer's occupation of the region.

Quantitative reconstruction of a single super rainstorm using daily resolved δ18O of land snail shells

A “once-in-a-millennium” super rainstorm battered Zhengzhou, central China, from 07/17/2021 to 07/22/2021 (named “7.20” Zhengzhou rainstorm). It killed 398 people and caused billions of dollars in damage. A pressing question is whether rainstorms of this intensity can be effectively documented by geological archives to understand better their historical variabilities beyond the range of meteorological data. Here, four land snail shells were collected from Zhengzhou, and weekly to daily resolved snail shell δ18O records from June to September of 2021 were obtained by gas-source mass spectrometry and secondary ion mass spectrometry. The daily resolved records show a dramatic negative shift between 06/18/2021 and 09/18/2021, which has been attributed to the “7.20” Zhengzhou rainstorm. Moreover, the measured amplitude of this shift is consistent with the theoretical value estimated from the flux balance model and instrumental data for the “7.20” Zhengzhou rainstorm. Our results suggest that the ultra-high resolution δ18O of land snail shells have the potential to reconstruct local synoptic scale rainstorms quantitatively, and thus fossil snail shells in sedimentary strata can be valuable material for investigating the historical variability of local rainstorms under different climate backgrounds.

Distribution of branched glycerol dialkyl glycerol tetraether (brGDGT) lipids from soils and sediments from the same watershed are distinct regionally (central Chile) but not globally

Quantitative reconstructions of past continental climates are vital for understanding contemporary and past climate change. Branched glycerol dialkyl glycerol tetraethers (brGDGTs) are unique bacterial lipids that have been proposed as universal paleothermometers due to their correlation with temperature in modern settings. Thus, brGDGTs may serve as a crucial paleotemperature proxy for understanding past climate variations and improving regional climate projections, especially in critical but under constrained regions. That said, complications can arise in their application due to varying source contributions (e.g., soils vs. peats vs. lacustrine). As such, this study investigates brGDGT distributions in Chilean lake surface sediments and corresponding watershed soils to determine the source of brGDGTs to lake sediments. Global datasets of brGDGTs in lake sediments and soils were additionally compiled for comparison. Distinct brGDGT distributions in Chilean lakes and soils indicate minimal bias from soil inputs to the lacustrine sediments as well as in situ lacustrine production of brGDGTs, which supports the use of brGDGTs in lake sediments as reliable paleotemperature proxies in the region. The ΣIIIa/ΣIIa ratio, initially promising as a brGDGT source indicator in marine settings, shows global complexities in lacustrine settings, challenging the establishment of universal thresholds for source apportionment. That said, we show that the ratio can be successfully applied in Chilean lake surface sediments. Direct comparisons with watershed soils and further research are crucial for discerning brGDGT sources in lake sediments and improving paleotemperature reconstructions on regional and global scales moving forward. Overall, this study contributes valuable insights into brGDGT variability, essential for accurate paleoreconstructions.

The Quantitative Reconstruction of Paleoclimate in the Huangling Region of the Chinese Loess Plateau during the Middle and Late Holocene

The Huangling region is located in the central part of the Chinese Loess Plateau, which is sensitive to climate change due to the transitional characteristics of the natural environmental zone in which it is located. In this study, we utilized a spore–pollen analysis of the Tianjiahe (TJH) profile in Huangling to apply the pollen–climate factor conversion function method. This approach allowed us to quantitatively reconstruct the paleotemperature and paleoprecipitation of the Huangling area during the Middle and Late Holocene. The results show that the Huangling area experienced four climatic stages during the Middle and Late Holocene, including mild and slightly humid → warm and humid → warm and slightly humid → warm and humid. Except for the period of 5.3–4.72 kaBP, during which the climate was relatively cool and dry compared to the present, the climate in the remaining period (4.72–0.03 kaBP) was warmer and more humid than that of the present. The above results provide an important insight for further exploring the mechanism of paleoclimate change and predicting future climate change.

Influences of burial process on diagenesis and high-quality reservoir development of deep–ultra-deep clastic rocks: A case study of Lower Cretaceous Qingshuihe Formation in southern margin of Junggar Basin, NW China

Taking the Lower Cretaceous Qingshuihe Formation in the southern margin of Junggar Basin as an example, the influences of the burial process in a foreland basin on the diagenesis and the development of high-quality reservoirs of deep and ultra-deep clastic rocks were investigated using thin section, scanning electron microscope, electron probe, stable isotopic composition and fluid inclusion data. The Qingshuihe Formation went through four burial stages of slow shallow burial, tectonic uplift, progressive deep burial and rapid deep burial successively. The stages of slow shallow burial and tectonic uplift not only can alleviate the mechanical compaction of grains, but also can maintain an open diagenetic system in the reservoirs for a long time, which promotes the dissolution of soluble components by meteoric freshwater and inhibits the precipitation of dissolution products in the reservoirs. The late rapid deep burial process contributed to the development of fluid overpressure, which effectively inhibits the destruction of primary pores by compaction and cementation. The fluid overpressure promotes the development of microfractures in the reservoir, which enhances the dissolution effect of organic acids. Based on the quantitative reconstruction of porosity evolution history, it is found that the long-term slow shallow burial and tectonic uplift processes make the greatest contribution to the development of deep–ultra-deep high-quality clastic rock reservoirs, followed by the late rapid deep burial process, and the progressive deep burial process has little contribution.

Effects of varied modern pollen-climate calibration-set establishment approaches on quantitative climate reconstructions

A representative modern calibration dataset is an essential prerequisite for a reliable quantitative reconstruction of paleoclimate. However, there has been a notable lack of systematic research on the optimal filtering of calibration sets. Four representative high-resolution pollen records were selected to repeatedly reconstruct paleoclimate using multiple approaches such as geographical distance, analogues and climate gradient. Comparisons of pollen-based reconstructions indicate that these varied approaches exhibit significant discrepancy in model performance and reconstruction accuracy under different geographical circumstances and time scales. Generally, in terms of calibration-set selection, the geographical distance method demonstrates greater efficiency for the plain region, but exhibit limitations in areas characterized by significant topographic changes. Analogue-calibration has been found to be more suitable for the edge of the database and also has been proven superior to other two methods for reconstructions exceed Holocene. Selecting calibration sets based only on climate gradients is inappropriate for independent applications, but may act as a limitation for other methods. This preliminary research can contribute to a more precise projection of potential future climate changes by providing essential data input or validating hindcasts for Earth system models.

A novel approach for quantitatively distinguishing between anthropogenic and natural effects on paleovegetation.

How to distinguish and quantify past human impacts on vegetation is a significant challenge in paleoecology. Here, we propose a novel method, the error inflection point-discriminant technique. It finds out the inflection points (IPs) of the regression errors of pollen-climate transfer functions using modern pollen spectra from vegetation with different values of the Human Influence Index (HII), which represent the HII threshold values of native/secondary and secondary/artificial vegetation systems. Our results show that the HII value at the native/secondary vegetation IPs is approximately 22 and globally uniform, whereas it varies regionally for the secondary/artificial vegetation IPs. In a case study of the Liangzhu archaeological site in the lower Yangtze River, discriminant functions for pollen spectra from three vegetation types and pollen-climate transfer functions of the native vegetation were established to reconstruct paleovegetation and paleoclimate over the past 6,600 years. Our study demonstrates this method's feasibility for quantitatively distinguishing human impacts on paleovegetation and assessing quantitative paleoclimate reconstructions using pollen data.

Late Holocene climate dynamics in the Azores archipelago

The location of the Azores Archipelago makes this group of islands an excellent setting for investigating past long-term temperature and precipitation changes in the central North Atlantic region. Here, we present a chironomid-based quantitative temperature reconstruction and a record of oxygen isotope composition of chironomid head capsules for the last ca. 1200 years, based on the Lake Prata core (São Miguel Island, Azores). Chironomids from Lake Prata show that the temperature trend is aligned with the prevailing past global temperature trends, indicating warming during the Medieval Climate Anomaly (MCA; ca. 950–1300 CE) and subsequent cooling during the Little Ice Age (LIA; ca. 1300–1800 CE). Furthermore, changes in lake water oxygen isotope composition from chironomid head capsules were recorded with more positive δ18O values (18.2 ± 0.2‰) during the MCA and more negative values (varying between 14.8‰ and 17.6‰) during the LIA, indicating a positive shift in the precipitation-evaporation balance and/or heavier precipitation in winter relative to summer. Our study revealed that, in subtropical regions with a temperate oceanic climate and low thermal variation, there is a limited correlation between the isotopic composition of precipitation and temperature. Additionally, the temporal sequence of oxygen isotope values obtained from chironomid head capsules closely corresponds to variations in precipitation seasonality inferred from distinct climatic proxies. This consistency underscores the robustness of quantitative reconstructions and indicates that δ18Ochironomid records are promising for future investigations, especially in the context of lakes located on oceanic islands characterised by limited thermal variability.

Two chironomid-inferred mean July air temperature reconstructions in the South Carpathian Mountains over the last 2000 years

We present chironomid-based reconstructions of mean July air temperature changes over the last 2000 years from Lake Latoriței (1530 m a.s.l.) in the Southern Carpathians. A multi-proxy analysis was performed along a 58 cm long sediment core and two training sets were used for quantitative July air temperature reconstructions: the Eastern-European (EE, 212 lakes) and the Finnish-Polish-Carpathian (FPC, 273 lakes). The transfer functions had a coefficient of determination ( r2) 0.88 and 0.91 with a root mean squared error of prediction (RMSEP) 0.88°C and 1.02°C. Despite possible biases resulting from methodological problems and the ecological complexity of the chironomid response to both climatic and environmental changes, the agreement of the temperature reconstruction of Lake Latoriței with other alpine records suggests that the transfer function successfully reconstructed past summer temperatures between 750 and 1830 CE. Biases in the temperature reconstruction in the period before 750 and after 1830 CE were likely caused by increased abundance of rheophilic and semi-terrestrial chironomid species related to increased inflow activity before 750 CE and local land use changes after 1830 CE, which was also indicated by increasing deforestation and increasing lake productivity in the pollen and diatom records. Our results suggest that the region experienced a warm period between 750 and 1360 CE, and a cold period between 1360 and 1600 CE followed by fluctuating summer temperatures until 1830 CE. These events were associated with the so-called ‘Mediaeval Warm Period’ (MWP) and the ‘Little Ice Age’ (LIA), respectively. The inference models reconstructed a decrease in July air temperatures by 0.7°C–1.1°C during the LIA relative to the warmer MWP. We also demonstrated that the FPC training set gives better results, supporting that local/continental training sets are efficient to detect weak amplitude summer temperature changes in the Late-Holocene.

Hydroclimate Changes Based on Testate Amoebae in the Greater Khingan Mountains’ Peatland (NE China) during the Last Millennium

The driving force of climate change in the monsoon margin is complex, making it a key area for regional and global climate change research. Palaeohydrological studies in the monsoon margin have increased the resolution of research in the long term, transitioning from qualitative to quantitative studies to comprehend climate change processes, patterns, and mechanisms. Testate amoebae (TA) in peat sediments are used as a proxy indicator organism for quantitative reconstruction of palaeohydrology. Thus, their community changes are directly related to precipitation, and widely used to reconstruct the patterns of summer precipitation globally. We investigated TA species and reconstructed palaeohydrological changes in the Greater Khingan Mountains’ Hongtu (HT) peatland, located in the East Asian Summer Monsoon (EASM) margin. The result showed that the most abundant TA species were Assulina muscorum (12.4 ± 5.0%) and Nebela tincta (8.9 ± 4.9%) in the HT peat core. The increase in dry indicator species (e.g., A. muscorum and Alabasta militaris) indicated a drying pattern in the HT peatland since 150 cal yr BP. Principal component analysis (PCA) explained 47.6% of the variation in the selected TA assemblages. During 400 to 250 cal yr BP, PCA axis 1 scores ranged from 0.2 to −1.3 (reflecting a drier climate), associating with the Little Ice Age. The paleohydrology of the northern part of the Greater Khingan Mountains was mainly controlled by the EASM, which was associated with changes in North Atlantic Sea surface temperature and solar radiative forcing. The apparent drying pattern may be the result of the gradual intensification of anthropogenic activities and the increase in EASM intensity.

Quantitative passive imaging by iterative holography: the example of helioseismic holography

In passive imaging, one attempts to reconstruct some coefficients in a wave equation from correlations of observed randomly excited solutions to this wave equation. Many methods proposed for this class of inverse problem so far are only qualitative, e.g. trying to identify the support of a perturbation. Major challenges are the increase in dimensionality when computing correlations from primary data in a preprocessing step, and often very poor pointwise signal-to-noise ratios. In this paper, we propose an approach that addresses both of these challenges: it works only on the primary data while implicitly using the full information contained in the correlation data, and it provides quantitative estimates and convergence by iteration. Our work is motivated by helioseismic holography, a well-established imaging method to map heterogenities and flows in the solar interior. We show that the back-propagation used in classical helioseismic holography can be interpreted as the adjoint of the Fréchet derivative of the operator which maps the properties of the solar interior to the correlation data on the solar surface. The theoretical and numerical framework for passive imaging problems developed in this paper extends helioseismic holography to nonlinear problems and allows for quantitative reconstructions. We present a proof of concept in uniform media.